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Protein

Volume-regulated anion channel subunit LRRC8D

Gene

Lrrc8d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5223345 Miscellaneous transport and binding events

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8D
Alternative name(s):
Leucine-rich repeat-containing protein 5
Leucine-rich repeat-containing protein 8D
Gene namesi
Name:Lrrc8d
Synonyms:Lrrc5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1922368 Lrrc8d

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 164ExtracellularSequence analysisAdd BLAST121
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 319CytoplasmicSequence analysisAdd BLAST134
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 365ExtracellularSequence analysisAdd BLAST25
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 859CytoplasmicSequence analysisAdd BLAST473

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000844941 – 859Volume-regulated anion channel subunit LRRC8DAdd BLAST859

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 355By similarity
Modified residuei242PhosphoserineBy similarity1
Modified residuei243PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ8BGR2
MaxQBiQ8BGR2
PaxDbiQ8BGR2
PRIDEiQ8BGR2

PTM databases

iPTMnetiQ8BGR2
PhosphoSitePlusiQ8BGR2

Expressioni

Gene expression databases

BgeeiENSMUSG00000046079 Expressed in 250 organ(s), highest expression level in utricle of membranous labyrinth
CleanExiMM_LRRC8D
ExpressionAtlasiQ8BGR2 baseline and differential
GenevisibleiQ8BGR2 MM

Interactioni

Subunit structurei

Heterohexamer (Probable). Oligomerizes with other LRRC8 proteins (LRRC8A, LRRC8B, LRRC8C and/or LRRC8E) to form a heterohexamer. In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (PubMed:24782309).Curated1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057293

Structurei

3D structure databases

ProteinModelPortaliQ8BGR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati515 – 535LRR 1Add BLAST21
Repeati539 – 560LRR 2Add BLAST22
Repeati562 – 583LRR 3Add BLAST22
Repeati590 – 610LRR 4Add BLAST21
Repeati613 – 633LRR 5Add BLAST21
Repeati637 – 658LRR 6Add BLAST22
Repeati660 – 681LRR 7Add BLAST22
Repeati685 – 706LRR 8Add BLAST22
Repeati708 – 729LRR 9Add BLAST22
Repeati731 – 752LRR 10Add BLAST22
Repeati754 – 775LRR 11Add BLAST22
Repeati777 – 798LRR 12Add BLAST22
Repeati800 – 821LRR 13Add BLAST22

Sequence similaritiesi

Belongs to the LRRC8 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00930000150868
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ8BGR2
KOiK22038
OMAiQLFKCVK
OrthoDBiEOG091G01ER
PhylomeDBiQ8BGR2
TreeFamiTF331443

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 2 hits
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 9 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8BGR2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFTLAEVASL NDIQPTYRIL KPWWDVFMDY LAVVMLMVAI FAGTMQLTKD
60 70 80 90 100
QVVCLPVLPS PANSKAHTPP GNADITTEVP RMETATHQDQ NGQTTTNDVA
110 120 130 140 150
FGTSAVTPDI PLQATHPHAE STLPNQEAKK EKRDPTGRKT NLDFQQYVFI
160 170 180 190 200
NQMCYHLALP WYSKYFPYLA LIHTIILMVS SNFWFKYPKT CSKVEHFVSI
210 220 230 240 250
LGKCFESPWT TKALSETACE DSEENKQRIT GAQTLPKHVS TSSDEGSPSA
260 270 280 290 300
STPMINKTGF KFSAEKPVIE VPSMTILDKK DGEQAKALFE KVRKFRAHVE
310 320 330 340 350
DSDLIYKLYV VQTLIKTAKF IFILCYTANF VNAISFEHVC KPKVEHLTGY
360 370 380 390 400
EVFECTHNMA YMLKKLLISY ISIICVYGFI CLYTLFWLFR IPLKEYSFEK
410 420 430 440 450
VREESSFSDI PDVKNDFAFL LHMVDQYDQL YSKRFGVFLS EVSENKLREI
460 470 480 490 500
SLNHEWTFEK LRQHVSRNAQ DKQELHLFML SGVPDAVFDL TDLDVLKLEL
510 520 530 540 550
IPEAKIPAKI SQMTNLQELH LCHCPAKVEQ TAFSFLRDHL RCLHVKFTDV
560 570 580 590 600
AEIPAWVYLL KNLRELYLIG NLNSENNKMI GLESLRELRH LKILHVKSNL
610 620 630 640 650
TKVPSNITDV APHLTKLVIH NDGTKLLVLN SLKKMMNVAE LELQNCELER
660 670 680 690 700
IPHAIFSLSN LQELDLKSNN IRTIEEIISF QHLKRLTCLK LWHNKIVAIP
710 720 730 740 750
PSITHVKNLE SLYFSNNKLE SLPTAVFSLQ KLRCLDVSYN NISTIPIEIG
760 770 780 790 800
LLQNLQHLHI TGNKVDILPK QLFKCVKLRT LNLGQNCIAS LPEKISQLTQ
810 820 830 840 850
LTQLELKGNC LDRLPAQLGQ CRMLKKSGLV VEDQLFDTLP LEVKEALNQD

VNVPFANGI
Length:859
Mass (Da):98,113
Last modified:March 1, 2003 - v1
Checksum:i7A8DD3CF52AB982D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YV62D3YV62_MOUSE
Volume-regulated anion channel subu...
Lrrc8d
93Annotation score:
D3Z5Q6D3Z5Q6_MOUSE
Volume-regulated anion channel subu...
Lrrc8d
37Annotation score:
D3Z6C1D3Z6C1_MOUSE
Volume-regulated anion channel subu...
Lrrc8d
41Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75I → V in AAH37717 (PubMed:15489334).Curated1
Sequence conflicti128A → V in AAH37717 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046249 mRNA Translation: BAC32656.1
AK050400 mRNA Translation: BAC34237.1
AK080722 mRNA Translation: BAC37994.1
AK137459 mRNA Translation: BAE23361.1
BC037717 mRNA Translation: AAH37717.1
CCDSiCCDS19494.1
RefSeqiNP_001116240.1, NM_001122768.1
NP_848816.3, NM_178701.3
XP_006534956.1, XM_006534893.2
XP_006534957.1, XM_006534894.3
XP_006534958.1, XM_006534895.3
XP_006534959.1, XM_006534896.3
XP_011247738.1, XM_011249436.1
UniGeneiMm.23837

Genome annotation databases

EnsembliENSMUST00000060531; ENSMUSP00000057293; ENSMUSG00000046079
ENSMUST00000120847; ENSMUSP00000113603; ENSMUSG00000046079
GeneIDi231549
KEGGimmu:231549
UCSCiuc008ylg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046249 mRNA Translation: BAC32656.1
AK050400 mRNA Translation: BAC34237.1
AK080722 mRNA Translation: BAC37994.1
AK137459 mRNA Translation: BAE23361.1
BC037717 mRNA Translation: AAH37717.1
CCDSiCCDS19494.1
RefSeqiNP_001116240.1, NM_001122768.1
NP_848816.3, NM_178701.3
XP_006534956.1, XM_006534893.2
XP_006534957.1, XM_006534894.3
XP_006534958.1, XM_006534895.3
XP_006534959.1, XM_006534896.3
XP_011247738.1, XM_011249436.1
UniGeneiMm.23837

3D structure databases

ProteinModelPortaliQ8BGR2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057293

PTM databases

iPTMnetiQ8BGR2
PhosphoSitePlusiQ8BGR2

Proteomic databases

EPDiQ8BGR2
MaxQBiQ8BGR2
PaxDbiQ8BGR2
PRIDEiQ8BGR2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060531; ENSMUSP00000057293; ENSMUSG00000046079
ENSMUST00000120847; ENSMUSP00000113603; ENSMUSG00000046079
GeneIDi231549
KEGGimmu:231549
UCSCiuc008ylg.2 mouse

Organism-specific databases

CTDi55144
MGIiMGI:1922368 Lrrc8d

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00930000150868
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ8BGR2
KOiK22038
OMAiQLFKCVK
OrthoDBiEOG091G01ER
PhylomeDBiQ8BGR2
TreeFamiTF331443

Enzyme and pathway databases

ReactomeiR-MMU-5223345 Miscellaneous transport and binding events

Miscellaneous databases

ChiTaRSiLrrc8d mouse
PROiPR:Q8BGR2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046079 Expressed in 250 organ(s), highest expression level in utricle of membranous labyrinth
CleanExiMM_LRRC8D
ExpressionAtlasiQ8BGR2 baseline and differential
GenevisibleiQ8BGR2 MM

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 2 hits
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 9 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLRC8D_MOUSE
AccessioniPrimary (citable) accession number: Q8BGR2
Secondary accession number(s): Q3UVA9, Q8CI13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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