UniProtKB - Q8BGG7 (UBS3B_MOUSE)
Ubiquitin-associated and SH3 domain-containing protein B
Ubash3b
Functioni
Catalytic activityi
- EC:3.1.3.481 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 379 | 1 | ||
Active sitei | 380 | Tele-phosphohistidine intermediate | 1 | |
Active sitei | 565 | 1 |
GO - Molecular functioni
- identical protein binding Source: MGI
- phosphoprotein binding Source: MGI
- protein tyrosine phosphatase activity Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- collagen-activated signaling pathway Source: MGI
- collagen-activated tyrosine kinase receptor signaling pathway Source: MGI
- negative regulation of bone resorption Source: MGI
- negative regulation of osteoclast differentiation Source: MGI
- negative regulation of platelet aggregation Source: MGI
- negative regulation of protein kinase activity Source: MGI
- negative regulation of signal transduction Source: MGI
- peptidyl-tyrosine dephosphorylation Source: MGI
- platelet activation Source: MGI
- platelet aggregation Source: MGI
- regulation of osteoclast differentiation Source: CACAO
- regulation of protein binding Source: MGI
- regulation of release of sequestered calcium ion into cytosol Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)Alternative name(s): Cbl-interacting protein p70 Suppressor of T-cell receptor signaling 1 Short name: STS-1 T-cell ubiquitin ligand 2 Short name: TULA-2 Tyrosine-protein phosphatase STS1/TULA2 |
Gene namesi | Name:Ubash3b Synonyms:Sts1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920078, Ubash3b |
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245509 | 1 – 638 | Ubiquitin-associated and SH3 domain-containing protein BAdd BLAST | 638 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Modified residuei | 12 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8BGG7 |
PaxDbi | Q8BGG7 |
PeptideAtlasi | Q8BGG7 |
PRIDEi | Q8BGG7 |
PTM databases
iPTMneti | Q8BGG7 |
PhosphoSitePlusi | Q8BGG7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000032020, Expressed in dorsal root ganglion and 237 other tissues |
ExpressionAtlasi | Q8BGG7, baseline and differential |
Genevisiblei | Q8BGG7, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:20516590, PubMed:17679096).
Interacts with JAK2 (in vitro) (PubMed:12370296).
Interacts with CBL.
Part of a complex containing CBL and activated EGFR.
Interacts with ubiquitin and with mono-ubiquitinated proteins (By similarity).
Interacts with ZAP70 (ubiquitinated form) (PubMed:26903241).
By similarity4 PublicationsBinary interactionsi
Q8BGG7
With | #Exp. | IntAct |
---|---|---|
ZAP70 [P43403] from Homo sapiens. | 10 | EBI-8846415,EBI-1211276 |
GO - Molecular functioni
- identical protein binding Source: MGI
- phosphoprotein binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 215592, 23 interactors |
IntActi | Q8BGG7, 4 interactors |
MINTi | Q8BGG7 |
STRINGi | 10090.ENSMUSP00000043865 |
Miscellaneous databases
RNActi | Q8BGG7, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q8BGG7 |
SMRi | Q8BGG7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8BGG7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 65 | UBAPROSITE-ProRule annotationAdd BLAST | 43 | |
Domaini | 243 – 308 | SH3PROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 369 – 638 | Protein tyrosine phosphataseAdd BLAST | 270 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 325 – 328 | Poly-Ser | 4 |
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3734, Eukaryota |
GeneTreei | ENSGT00940000156097 |
HOGENOMi | CLU_016516_1_0_1 |
InParanoidi | Q8BGG7 |
OMAi | DECGTWV |
OrthoDBi | 243749at2759 |
PhylomeDBi | Q8BGG7 |
TreeFami | TF313334 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit cd11936, SH3_UBASH3B, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015940, UBA IPR009060, UBA-like_sf IPR035632, UBASH3B_SH3 |
Pfami | View protein in Pfam PF00300, His_Phos_1, 1 hit PF14604, SH3_9, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF50044, SSF50044, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50030, UBA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAREELYSK VTPRRDRLQR PGTVKHGSAL DVLLSMGFPR ARAQKALAST
60 70 80 90 100
GGRSVQAACD WLFSHVGDPF LDDPLPREYV LYLRPTGPLA QKLSDFWQQS
110 120 130 140 150
KQICGKNKAH NIFPHITLCQ FFMCEDSKVD ALGEALQTTV SRWKCKFSAP
160 170 180 190 200
LPLELYTSSN FIGLFVKEDS AEVLKKFAAD FAAEAASKTE VHVEPHKKQL
210 220 230 240 250
HVTLAYHFQA SHLPTLEKLA QNIDVKLGCD WVATIFSRDI RFANHETLQV
260 270 280 290 300
IYPYSPQNDD ELELVPGDFI FMSPMEQTST SEGWIYGTSL TTGCSGLLPE
310 320 330 340 350
NYITKADECS TWIFHGSYSI LNTVSSSSLA FGDGALERRQ YEDQGLGETT
360 370 380 390 400
PLTIICQPMQ PLRVNSQPGP QKRCLFVCRH GERMDVVFGK YWLSQCFDAK
410 420 430 440 450
GRYIRTNLNM PHSLPQRSGG FRDYEKDAPI TVFGCMQARL VGEALLESNT
460 470 480 490 500
VIDHVYCSPS LRCVQTAHNI LKGLQQDNHL KIRVEPGLFE WTKWVAGSTL
510 520 530 540 550
PAWIPPSELA AANLSVDTTY RPHIPVSKLA ISESYDTYIN RSFQVTKEII
560 570 580 590 600
SECKSKGNNI LIVAHASSLE ACTCQLQGLS PQNSKDFVQM VRKIPYLGFC
610 620 630
SCEELGETGI WQLTDPPILP LTHGPTGGFN WRETLLQE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YVT5 | D3YVT5_MOUSE | Ubiquitin-associated and SH3 domain... | Ubash3b | 116 | Annotation score: | ||
H3BJB9 | H3BJB9_MOUSE | Ubiquitin-associated and SH3 domain... | Ubash3b | 93 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 300 | E → G in BAE30779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 300 | E → G in BAE30875 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019716 | 1 – 122 | Missing in isoform 2. 1 PublicationAdd BLAST | 122 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075602 mRNA Translation: BAD06450.1 AK013361 mRNA Translation: BAC25403.1 AK034450 mRNA Translation: BAC28714.1 AK035764 mRNA Translation: BAC29178.1 AK133948 mRNA Translation: BAE21945.1 AK151895 mRNA Translation: BAE30779.1 AK152013 mRNA Translation: BAE30875.1 AK154576 mRNA Translation: BAE32688.1 BC053436 mRNA Translation: AAH53436.1 |
CCDSi | CCDS23085.1 [Q8BGG7-1] CCDS90541.1 [Q8BGG7-2] |
RefSeqi | NP_789830.1, NM_176860.5 [Q8BGG7-1] XP_006510700.1, XM_006510637.3 XP_017169101.1, XM_017313612.1 [Q8BGG7-2] |
Genome annotation databases
Ensembli | ENSMUST00000044155; ENSMUSP00000043865; ENSMUSG00000032020 [Q8BGG7-1] ENSMUST00000151485; ENSMUSP00000116038; ENSMUSG00000032020 [Q8BGG7-2] |
GeneIDi | 72828 |
KEGGi | mmu:72828 |
UCSCi | uc009pag.1, mouse [Q8BGG7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075602 mRNA Translation: BAD06450.1 AK013361 mRNA Translation: BAC25403.1 AK034450 mRNA Translation: BAC28714.1 AK035764 mRNA Translation: BAC29178.1 AK133948 mRNA Translation: BAE21945.1 AK151895 mRNA Translation: BAE30779.1 AK152013 mRNA Translation: BAE30875.1 AK154576 mRNA Translation: BAE32688.1 BC053436 mRNA Translation: AAH53436.1 |
CCDSi | CCDS23085.1 [Q8BGG7-1] CCDS90541.1 [Q8BGG7-2] |
RefSeqi | NP_789830.1, NM_176860.5 [Q8BGG7-1] XP_006510700.1, XM_006510637.3 XP_017169101.1, XM_017313612.1 [Q8BGG7-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2H0Q | X-ray | 1.82 | A/B/C | 373-633 | [»] | |
2IKQ | X-ray | 2.61 | A/B/M | 369-638 | [»] | |
3MBK | X-ray | 1.35 | A/B | 373-636 | [»] | |
BMRBi | Q8BGG7 | |||||
SMRi | Q8BGG7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 215592, 23 interactors |
IntActi | Q8BGG7, 4 interactors |
MINTi | Q8BGG7 |
STRINGi | 10090.ENSMUSP00000043865 |
PTM databases
iPTMneti | Q8BGG7 |
PhosphoSitePlusi | Q8BGG7 |
Proteomic databases
EPDi | Q8BGG7 |
PaxDbi | Q8BGG7 |
PeptideAtlasi | Q8BGG7 |
PRIDEi | Q8BGG7 |
Protocols and materials databases
Antibodypediai | 32801, 136 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044155; ENSMUSP00000043865; ENSMUSG00000032020 [Q8BGG7-1] ENSMUST00000151485; ENSMUSP00000116038; ENSMUSG00000032020 [Q8BGG7-2] |
GeneIDi | 72828 |
KEGGi | mmu:72828 |
UCSCi | uc009pag.1, mouse [Q8BGG7-1] |
Organism-specific databases
CTDi | 84959 |
MGIi | MGI:1920078, Ubash3b |
Phylogenomic databases
eggNOGi | KOG3734, Eukaryota |
GeneTreei | ENSGT00940000156097 |
HOGENOMi | CLU_016516_1_0_1 |
InParanoidi | Q8BGG7 |
OMAi | DECGTWV |
OrthoDBi | 243749at2759 |
PhylomeDBi | Q8BGG7 |
TreeFami | TF313334 |
Miscellaneous databases
BioGRID-ORCSi | 72828, 0 hits in 19 CRISPR screens |
ChiTaRSi | Ubash3b, mouse |
EvolutionaryTracei | Q8BGG7 |
PROi | PR:Q8BGG7 |
RNActi | Q8BGG7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032020, Expressed in dorsal root ganglion and 237 other tissues |
ExpressionAtlasi | Q8BGG7, baseline and differential |
Genevisiblei | Q8BGG7, MM |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit cd11936, SH3_UBASH3B, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015940, UBA IPR009060, UBA-like_sf IPR035632, UBASH3B_SH3 |
Pfami | View protein in Pfam PF00300, His_Phos_1, 1 hit PF14604, SH3_9, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF50044, SSF50044, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50030, UBA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBS3B_MOUSE | |
Accessioni | Q8BGG7Primary (citable) accession number: Q8BGG7 Secondary accession number(s): Q3U8Z2, Q8BMW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references