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Protein

Fanconi anemia group M protein homolog

Gene

Fancm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates. In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 106ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6783310 Fanconi Anemia Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Fanconi anemia group M protein homolog (EC:3.6.4.13By similarity)
Short name:
Protein FACM
Alternative name(s):
ATP-dependent RNA helicase FANCM
Gene namesi
Name:Fancm
Synonyms:Kiaa1596
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442306 Fancm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176806

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551771 – 2021Fanconi anemia group M protein homologAdd BLAST2021

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30PhosphoserineBy similarity1
Modified residuei1637PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated; hyperphosphorylated in response to genotoxic stress.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BGE5
PRIDEiQ8BGE5

PTM databases

iPTMnetiQ8BGE5
PhosphoSitePlusiQ8BGE5

Expressioni

Gene expression databases

BgeeiENSMUSG00000055884 Expressed in 162 organ(s), highest expression level in embryo
CleanExiMM_FANCM
ExpressionAtlasiQ8BGE5 baseline and differential
GenevisibleiQ8BGE5 MM

Interactioni

Subunit structurei

Component of the Fanconi anemia (FA) core complex, which consists of CENPS, CENPX, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, FANCM, FAAP24 and FAAP100. The FA core complex associates with Bloom syndrome (BLM) complex, which consists of at least BLM, DNA topoisomerase 3-alpha/TOP3A, RMI1/BLAP75, RPA1/RPA70 and RPA2/RPA32. This supercomplex between FA and BLM complexes has been called BRAFT. Forms a discrete complex with CENPS and CENPX, called FANCM-MHF; this interaction stimulates DNA binding and replication fork remodeling by FANCM and stabilizes the binding partners. Forms a heterodimer with FAAP24; this interaction increases FANCM single-stranded DNA-binding activity.By similarity

Protein-protein interaction databases

BioGridi222708, 1 interactor
STRINGi10090.ENSMUSP00000054797

Structurei

3D structure databases

ProteinModelPortaliQ8BGE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 254Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini437 – 612Helicase C-terminalPROSITE-ProRule annotationAdd BLAST176

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1689 – 2009Interaction with FAAP24By similarityAdd BLAST321

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 205DEAH boxPROSITE-ProRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0354 Eukaryota
KOG0442 Eukaryota
COG1111 LUCA
GeneTreeiENSGT00510000046789
HOGENOMiHOG000112498
HOVERGENiHBG081520
InParanoidiQ8BGE5
KOiK10896
OMAiGIMDGTK
OrthoDBiEOG091G042T
PhylomeDBiQ8BGE5
TreeFamiTF324610

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR006166 ERCC4_domain
IPR031879 FANCM-MHF-bd
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR011335 Restrct_endonuc-II-like
IPR010994 RuvA_2-like
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
PF16783 FANCM-MHF_bd, 1 hit
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00891 ERCC4, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF52540 SSF52540, 2 hits
SSF52980 SSF52980, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BGE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGRQRTLFQ TWGPSLVRGS GDSGCGQPRS PAMAEALPEE DDEVLLVAAY
60 70 80 90 100
EAERQLDPGD GGFCAAAGAL WIYPTNCPVR DYQLDISRSA LFCNTLVCLP
110 120 130 140 150
TGLGKTFIAA VVMYNFYRWF PSGKVVFMAP TKPLVTQQME ACFHVMGIPQ
160 170 180 190 200
SHMAEMTGST QAVNRKEIWS SRRVLFLTPQ VMVNDLTRGA VPATHVKCLV
210 220 230 240 250
VDEAHKALGN YAYCQVVREL VKYTTHFRIL ALSATPGSDI KAVQQVITNL
260 270 280 290 300
LIGKIELRSE ESPDILPYSH ERRVEKLVVP LGEELGAIQK TYIQILETFA
310 320 330 340 350
SSLIHRNVLM KRDIPNLTKY QIILARDQFR KNPSPNIVGI QQGIIEGEFA
360 370 380 390 400
LCISLYHGYE LLQQMGMRSL YFFLSGIMDG TKGMTRARNE LSRNEDFMKL
410 420 430 440 450
YTHLQSAFAP ASTSDASAFQ RGNKEKKFVY SHPKLKKLEE VILEHFKSWN
460 470 480 490 500
AKATTEKKCH ESRVMIFSSF RDSVEEIAEM LLQHRPVIRV MTFVGHASGK
510 520 530 540 550
NTKGFTQKEQ LQVVRQFRDG GYNTLVSTCV GEEGLDIGEV DLIICFDAQK
560 570 580 590 600
SPIRLIQRMG RTGRKRQGRI VVILAEGREE RTYNQSQSNK KNIYKAISGN
610 620 630 640 650
RQVLRLYQGS PRMVPDKINP ELHKMYITHG VYEPEKARSV QRRPFSSRGG
660 670 680 690 700
IKASKSNKDG LLSEEEFNLW SRLYRLGDSD QVKGVALPQS HFPSLQEDRV
710 720 730 740 750
IQDPTTRIHQ LSLSEWSLWQ DRPLPTHQVD HSDRCHHFIS IMKMIEGMRH
760 770 780 790 800
EEGECSYELK IRPFLQMEDV CSKYHAPRNG YNNVASVASS AHQKSSFRPS
810 820 830 840 850
VDAGGSLTVI ESEEEHADTV KQRDSKWTKI TSLREKPCRA GRKGQTCEHS
860 870 880 890 900
EGEGEDGDAG SSDADGQSPA EADSQVDPPS GERMADVGGI SILGAVTEED
910 920 930 940 950
NHPGTLQMEC QVTNKSCARY SLDSGYSSFS DEKSVSSNLF LPLEEELFTD
960 970 980 990 1000
RAAEQFYNCR PMTEDVLANV ERFLSRSPPS LSGLSDLVYD VTQGCEFDNA
1010 1020 1030 1040 1050
SCSPYPEHEH SPRPVSPASH SAGNSQQNLE SNSAKRISHP TEKYCLPGTT
1060 1070 1080 1090 1100
HNKVSDRPSF CESDSEGHNI KYQNSGSNSC AQIQADLENN FVDKNSHDDS
1110 1120 1130 1140 1150
EPPVLFTDED ESLLLFEDDF KNIEDGPEEL NGASLPPFNS ISQPLRVSGK
1160 1170 1180 1190 1200
TLTSEMPPVS HFLISDELLL DDDSEPEDQI VCGAKSWKCQ EGVEDGQEEL
1210 1220 1230 1240 1250
RTDGQTFDCS VDLFSVTFDL GFRCSSGSDD EMLAGASDRT RTLGAADVSG
1260 1270 1280 1290 1300
RHSDKEIKDA GGASGPLGRA ISPIPTETAQ WSPWAQNKEY ASFHVASSSP
1310 1320 1330 1340 1350
VKQRVRSTPL SKSHASSKTG AHMLKTLDST KEKAGGQGFK MALNPRLGHL
1360 1370 1380 1390 1400
GFSVEETKSS DQVFVHQSPR RTEVEHLTSE SEDDVFLRKT KKPKRNVLKS
1410 1420 1430 1440 1450
PEDQKNNEVD SPIHAVKKPR VLRSELASSD DESENFGRTC PRLEHFKGRN
1460 1470 1480 1490 1500
RNIRKGSAAQ KNRSQVKTMA RRFLDDEAEV SGEDVDCVSA DEEDESENEQ
1510 1520 1530 1540 1550
DSSLLDFVND RTQLSQAIND SEMRAIYMKS VRSPLMSTKY RMVREKRPNM
1560 1570 1580 1590 1600
NIFSQIPEQD ENYLEDSFCV DEEESCKSQS SEEEISVDFN LTKDSFTDED
1610 1620 1630 1640 1650
IRYKTRYAVK IKQMNKKQNY TRPRKKLSRI ILPDDDSSEE ENIPKDREHS
1660 1670 1680 1690 1700
VAGGHAAAEH TQQGQLWASG PSGSSVPPQV LSDPSWNQSS RQRLQVQPSI
1710 1720 1730 1740 1750
TDAVPRTLNV KAQSHNKIKS ASPPCTGVES RKEYGNHPVQ LKADSQEHSD
1760 1770 1780 1790 1800
TSAAPCSTSL LHVAEGHTAP RHLQEGNRAC ILVDSREITT GLEVISSLRT
1810 1820 1830 1840 1850
VHGLQVEICP LNGCDYIVSS RMVVVRRSQS EMLSNTSKNK FIEQMQRLQS
1860 1870 1880 1890 1900
MFQRICVIVE KDREKAGDTS KKFRRTKCYD SLLTALVGAG IRILFSSGQE
1910 1920 1930 1940 1950
ETADLLKELS LVEQRKNAGI HIPAVLNTSK LEALPFYLSI PGISYITALN
1960 1970 1980 1990 2000
MCHQFSSVKK MANSSPEEIS TCAQVNHQKA EEIYKYIHYI FDMQMLPNDL
2010 2020
NQERQKPDTC LTLGVAMKEL S
Length:2,021
Mass (Da):226,018
Last modified:October 25, 2005 - v3
Checksum:i8646A4535437A8AF
GO
Isoform 2 (identifier: Q8BGE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     651-651: I → E
     652-2021: Missing.

Show »
Length:651
Mass (Da):73,449
Checksum:i8D80409F6155805A
GO
Isoform 3 (identifier: Q8BGE5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-433: NKEKKFVYSHP → LVFQGKLWSFT
     434-2021: Missing.

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):48,324
Checksum:iD8BE842C109AD622
GO
Isoform 4 (identifier: Q8BGE5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-741: Missing.

Note: No experimental confirmation available.
Show »
Length:1,280
Mass (Da):142,131
Checksum:iE57A47289A31A783
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VKF2A0A1Y7VKF2_MOUSE
Fanconi anemia group M protein homo...
Fancm
664Annotation score:
A0A1Y7VKY9A0A1Y7VKY9_MOUSE
Fanconi anemia group M protein homo...
Fancm
121Annotation score:
A0A1Y7VMA3A0A1Y7VMA3_MOUSE
Fanconi anemia group M protein homo...
Fancm
109Annotation score:
A0A1Y7VLB8A0A1Y7VLB8_MOUSE
Fanconi anemia group M protein homo...
Fancm
167Annotation score:

Sequence cautioni

The sequence BAD32489 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0159911 – 741Missing in isoform 4. 1 PublicationAdd BLAST741
Alternative sequenceiVSP_015992423 – 433NKEKKFVYSHP → LVFQGKLWSFT in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_015993434 – 2021Missing in isoform 3. 1 PublicationAdd BLAST1588
Alternative sequenceiVSP_015994651I → E in isoform 2. 1 Publication1
Alternative sequenceiVSP_015995652 – 2021Missing in isoform 2. 1 PublicationAdd BLAST1370

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050306 mRNA Translation: BAC34178.2
AK053715 mRNA Translation: BAC35487.1
AK084697 mRNA Translation: BAC39257.2
AK086395 mRNA Translation: BAC39660.1
AK173211 mRNA Translation: BAD32489.1 Different initiation.
CCDSiCCDS25941.1 [Q8BGE5-1]
RefSeqiNP_849243.2, NM_178912.3 [Q8BGE5-1]
XP_006515407.1, XM_006515344.3 [Q8BGE5-4]
UniGeneiMm.374847

Genome annotation databases

EnsembliENSMUST00000058889; ENSMUSP00000054797; ENSMUSG00000055884 [Q8BGE5-1]
GeneIDi104806
KEGGimmu:104806
UCSCiuc007nrc.1 mouse [Q8BGE5-3]
uc007nrd.1 mouse [Q8BGE5-2]
uc007nrf.1 mouse [Q8BGE5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050306 mRNA Translation: BAC34178.2
AK053715 mRNA Translation: BAC35487.1
AK084697 mRNA Translation: BAC39257.2
AK086395 mRNA Translation: BAC39660.1
AK173211 mRNA Translation: BAD32489.1 Different initiation.
CCDSiCCDS25941.1 [Q8BGE5-1]
RefSeqiNP_849243.2, NM_178912.3 [Q8BGE5-1]
XP_006515407.1, XM_006515344.3 [Q8BGE5-4]
UniGeneiMm.374847

3D structure databases

ProteinModelPortaliQ8BGE5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222708, 1 interactor
STRINGi10090.ENSMUSP00000054797

Chemistry databases

ChEMBLiCHEMBL2176806

PTM databases

iPTMnetiQ8BGE5
PhosphoSitePlusiQ8BGE5

Proteomic databases

PaxDbiQ8BGE5
PRIDEiQ8BGE5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058889; ENSMUSP00000054797; ENSMUSG00000055884 [Q8BGE5-1]
GeneIDi104806
KEGGimmu:104806
UCSCiuc007nrc.1 mouse [Q8BGE5-3]
uc007nrd.1 mouse [Q8BGE5-2]
uc007nrf.1 mouse [Q8BGE5-1]

Organism-specific databases

CTDi57697
MGIiMGI:2442306 Fancm
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0354 Eukaryota
KOG0442 Eukaryota
COG1111 LUCA
GeneTreeiENSGT00510000046789
HOGENOMiHOG000112498
HOVERGENiHBG081520
InParanoidiQ8BGE5
KOiK10896
OMAiGIMDGTK
OrthoDBiEOG091G042T
PhylomeDBiQ8BGE5
TreeFamiTF324610

Enzyme and pathway databases

ReactomeiR-MMU-6783310 Fanconi Anemia Pathway

Miscellaneous databases

PROiPR:Q8BGE5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055884 Expressed in 162 organ(s), highest expression level in embryo
CleanExiMM_FANCM
ExpressionAtlasiQ8BGE5 baseline and differential
GenevisibleiQ8BGE5 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR006166 ERCC4_domain
IPR031879 FANCM-MHF-bd
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR011335 Restrct_endonuc-II-like
IPR010994 RuvA_2-like
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
PF16783 FANCM-MHF_bd, 1 hit
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00891 ERCC4, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF52540 SSF52540, 2 hits
SSF52980 SSF52980, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFANCM_MOUSE
AccessioniPrimary (citable) accession number: Q8BGE5
Secondary accession number(s): Q69ZF5, Q8BKB7, Q8BUA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: September 12, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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