UniProtKB - Q8BGD9 (IF4B_MOUSE)
Protein
Eukaryotic translation initiation factor 4B
Gene
Eif4b
Organism
Mus musculus (Mouse)
Status
Functioni
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F (By similarity).By similarity
GO - Molecular functioni
- ribosomal small subunit binding Source: GO_Central
- RNA strand annealing activity Source: GO_Central
- RNA strand-exchange activity Source: GO_Central
- translation initiation factor activity Source: UniProtKB-KW
GO - Biological processi
- eukaryotic translation initiation factor 4F complex assembly Source: GO_Central
- formation of translation preinitiation complex Source: GO_Central
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-166208, mTORC1-mediated signalling R-MMU-429947, Deadenylation of mRNA R-MMU-72649, Translation initiation complex formation R-MMU-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-MMU-72702, Ribosomal scanning and start codon recognition R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 4BShort name: eIF-4B |
Gene namesi | Name:Eif4b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95304, Eif4b |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Other locations
- dendrite Source: MGI
- neuronal cell body Source: MGI
- postsynapse Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081617 | 1 – 611 | Eukaryotic translation initiation factor 4BAdd BLAST | 611 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 93 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineBy similarity | 1 | |
Modified residuei | 207 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 283 | PhosphoserineBy similarity | 1 | |
Cross-linki | 343 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 359 | PhosphoserineBy similarity | 1 | |
Modified residuei | 365 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 406 | PhosphoserineCombined sources | 1 | |
Modified residuei | 409 | PhosphoserineBy similarity | 1 | |
Modified residuei | 412 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 418 | PhosphoserineBy similarity | 1 | |
Modified residuei | 422 | PhosphoserineCombined sources | 1 | |
Modified residuei | 425 | PhosphoserineCombined sources | 1 | |
Modified residuei | 445 | PhosphoserineBy similarity | 1 | |
Modified residuei | 459 | PhosphoserineBy similarity | 1 | |
Modified residuei | 462 | PhosphoserineBy similarity | 1 | |
Modified residuei | 497 | PhosphoserineCombined sources | 1 | |
Modified residuei | 498 | PhosphoserineCombined sources | 1 | |
Modified residuei | 500 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 504 | PhosphoserineCombined sources | 1 | |
Modified residuei | 506 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 586 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 597 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3827 |
EPDi | Q8BGD9 |
jPOSTi | Q8BGD9 |
MaxQBi | Q8BGD9 |
PaxDbi | Q8BGD9 |
PRIDEi | Q8BGD9 |
ProteomicsDBi | 267262 |
PTM databases
iPTMneti | Q8BGD9 |
PhosphoSitePlusi | Q8BGD9 |
SwissPalmi | Q8BGD9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058655, Expressed in undifferentiated genital tubercle and 323 other tissues |
ExpressionAtlasi | Q8BGD9, baseline and differential |
Genevisiblei | Q8BGD9, MM |
Interactioni
Subunit structurei
Self-associates and interacts with EIF3 p170 subunit.
By similarityProtein-protein interaction databases
BioGRIDi | 217684, 24 interactors |
IntActi | Q8BGD9, 4 interactors |
MINTi | Q8BGD9 |
STRINGi | 10090.ENSMUSP00000127774 |
Miscellaneous databases
RNActi | Q8BGD9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 96 – 173 | RRMPROSITE-ProRule annotationAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 164 – 440 | Arg-richAdd BLAST | 277 | |
Compositional biasi | 169 – 325 | Asp-richAdd BLAST | 157 |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00940000153336 |
HOGENOMi | CLU_031798_0_0_1 |
InParanoidi | Q8BGD9 |
OMAi | KPPYTAH |
OrthoDBi | 1530583at2759 |
PhylomeDBi | Q8BGD9 |
TreeFami | TF101525 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR033107, EIF-4B IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23236:SF42, PTHR23236:SF42, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BGD9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAASAKKKNK KGKTISLTDF LAEDGGTGGG STYVPKPVSW ADETDDLEGD
60 70 80 90 100
VSTTWHSNDD DVYRAPPIDR SILPTAPRAA REPNIDRSRL PKSPPYTAFL
110 120 130 140 150
GNLPYDVTED SIKDFFRGLN ISAVRLPREP SNPDRLKGFG YAEFEDLDSL
160 170 180 190 200
LSALSLNEES LGNRRIRVDV ADQAQDKDRD DRSFGRDRNR DSDKTDTDWR
210 220 230 240 250
ARPTTDSFDD YPPRRGDDSF GDKYRDRYDS DRYRDGYRDG YRDGPRRDMD
260 270 280 290 300
RYGGRDRYDD RGSRDYDRGY DSRIGSGRRA FGSGYRRDDD YRGGGDRYED
310 320 330 340 350
RYDRRDDRSW SSRDDYSRDD YRRDDRGPPQ RPRLNLKPRS APKEDDASAS
360 370 380 390 400
TSQSSRAASI FGGAKPVDTA AREREVEERL QKEQEKLQRQ LDEPKLDRRP
410 420 430 440 450
RERHPSWRSE ETQERERSRT GSESSQTGAS ATSGRNTRRR ESEKSLENET
460 470 480 490 500
LNKEEDCHSP TSKPPKPDQP LKVMPAPPPK ENAWVKRSSN PPARSQSSDT
510 520 530 540 550
EQPSPTSGGG KVAAVQPPEE GPSRKDGNKV DVVGATQGQA GSCSRGPGDG
560 570 580 590 600
GSRDHWKDLD RKDGKKDQDS RSAPEPKKPE ENPASKFSSA SKYAALSVDG
610
EDEDEGDDCT E
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB2RWE8 | B2RWE8_MOUSE | Eif4b protein | Eif4b | 403 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK045250 mRNA Translation: BAC32280.1 AK077423 mRNA Translation: BAC36793.1 AK170354 mRNA Translation: BAE41740.1 |
CCDSi | CCDS49737.1 |
RefSeqi | NP_663600.2, NM_145625.3 |
Genome annotation databases
Ensembli | ENSMUST00000169681; ENSMUSP00000127774; ENSMUSG00000058655 |
GeneIDi | 75705 |
KEGGi | mmu:75705 |
UCSCi | uc007xuk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK045250 mRNA Translation: BAC32280.1 AK077423 mRNA Translation: BAC36793.1 AK170354 mRNA Translation: BAE41740.1 |
CCDSi | CCDS49737.1 |
RefSeqi | NP_663600.2, NM_145625.3 |
3D structure databases
SMRi | Q8BGD9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 217684, 24 interactors |
IntActi | Q8BGD9, 4 interactors |
MINTi | Q8BGD9 |
STRINGi | 10090.ENSMUSP00000127774 |
PTM databases
iPTMneti | Q8BGD9 |
PhosphoSitePlusi | Q8BGD9 |
SwissPalmi | Q8BGD9 |
Proteomic databases
CPTACi | non-CPTAC-3827 |
EPDi | Q8BGD9 |
jPOSTi | Q8BGD9 |
MaxQBi | Q8BGD9 |
PaxDbi | Q8BGD9 |
PRIDEi | Q8BGD9 |
ProteomicsDBi | 267262 |
Genome annotation databases
Ensembli | ENSMUST00000169681; ENSMUSP00000127774; ENSMUSG00000058655 |
GeneIDi | 75705 |
KEGGi | mmu:75705 |
UCSCi | uc007xuk.2, mouse |
Organism-specific databases
CTDi | 1975 |
MGIi | MGI:95304, Eif4b |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00940000153336 |
HOGENOMi | CLU_031798_0_0_1 |
InParanoidi | Q8BGD9 |
OMAi | KPPYTAH |
OrthoDBi | 1530583at2759 |
PhylomeDBi | Q8BGD9 |
TreeFami | TF101525 |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-166208, mTORC1-mediated signalling R-MMU-429947, Deadenylation of mRNA R-MMU-72649, Translation initiation complex formation R-MMU-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-MMU-72702, Ribosomal scanning and start codon recognition R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Miscellaneous databases
BioGRID-ORCSi | 75705, 10 hits in 52 CRISPR screens |
ChiTaRSi | Eif4b, mouse |
PROi | PR:Q8BGD9 |
RNActi | Q8BGD9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058655, Expressed in undifferentiated genital tubercle and 323 other tissues |
ExpressionAtlasi | Q8BGD9, baseline and differential |
Genevisiblei | Q8BGD9, MM |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR033107, EIF-4B IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23236:SF42, PTHR23236:SF42, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF4B_MOUSE | |
Accessioni | Q8BGD9Primary (citable) accession number: Q8BGD9 Secondary accession number(s): Q3TD64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot