UniProtKB - Q8BGD7 (NPAS4_MOUSE)
Neuronal PAS domain-containing protein 4
Npas4
Functioni
Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus (PubMed:18815592, PubMed:22194569, PubMed:23029555, PubMed:24201284, PubMed:24855953).
Plays a key role in the structural and functional plasticity of neurons (PubMed:23172225).
Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (PubMed:24201284, PubMed:24855953).
In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (PubMed:18815592, PubMed:22194569, PubMed:24201284).
In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (PubMed:24855953).
The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (PubMed:18815592, PubMed:22194569, PubMed:23029555, PubMed:24201284, PubMed:27238022).
Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (PubMed:25088421).
Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (PubMed:14701734, PubMed:15363889, PubMed:19284974).
Can activate the CME (CNS midline enhancer) element (PubMed:14701734, PubMed:15363889).
10 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- protein-containing complex binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- cellular response to corticosterone stimulus Source: MGI
- excitatory postsynaptic potential Source: UniProtKB
- inhibitory postsynaptic potential Source: UniProtKB
- inhibitory synapse assembly Source: UniProtKB
- learning Source: UniProtKB
- long-term memory Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of synaptic plasticity Source: UniProtKB
- regulation of synaptic transmission, GABAergic Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- short-term memory Source: UniProtKB
- social behavior Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal PAS domain-containing protein 4CuratedShort name: Neuronal PAS4Curated Alternative name(s): HLH-PAS transcription factor NXF1 Publication Limbic-enhanced PAS protein1 Publication Short name: LE-PAS1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2664186, Npas4 |
VEuPathDBi | HostDB:ENSMUSG00000045903 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation2 Publications
Nucleus
- nucleus Source: MGI
Other locations
- postsynapse Source: GOC
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248223 | 1 – 802 | Neuronal PAS domain-containing protein 4Add BLAST | 802 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q8BGD7 |
PRIDEi | Q8BGD7 |
ProteomicsDBi | 293945 |
PTM databases
PhosphoSitePlusi | Q8BGD7 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000045903, Expressed in islet of Langerhans and 81 other tissues |
ExpressionAtlasi | Q8BGD7, baseline and differential |
Genevisiblei | Q8BGD7, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 230436, 2 interactors |
STRINGi | 10090.ENSMUSP00000062992 |
Miscellaneous databases
RNActi | Q8BGD7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 53 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 70 – 144 | PAS 1PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 203 – 275 | PAS 2PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 280 – 319 | PACAdd BLAST | 40 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 13 | Basic motif; degeneratePROSITE-ProRule annotationAdd BLAST | 13 | |
Regioni | 14 – 53 | Helix-loop-helix motifPROSITE-ProRule annotationAdd BLAST | 40 | |
Regioni | 470 – 554 | DisorderedSequence analysisAdd BLAST | 85 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 5 – 38 | Sequence analysisAdd BLAST | 34 | |
Coiled coili | 624 – 648 | Sequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 470 – 514 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 529 – 554 | Polar residuesSequence analysisAdd BLAST | 26 |
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502QRXX, Eukaryota |
GeneTreei | ENSGT00530000064165 |
HOGENOMi | CLU_013890_0_0_1 |
InParanoidi | Q8BGD7 |
OMAi | KTYFTQE |
OrthoDBi | 219290at2759 |
PhylomeDBi | Q8BGD7 |
TreeFami | TF319684 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00091, PAS, 2 hits |
SUPFAMi | SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MYRSTKGASK ARRDQINAEI RNLKELLPLA EADKVRLSYL HIMSLACIYT
60 70 80 90 100
RKGVFFAGGT PLAGPTGLLS AQELEDIVAA LPGFLLVFTA EGKLLYLSES
110 120 130 140 150
VSEHLGHSMV DLVAQGDSIY DIIDPADHLT VRQQLTMPSA LDADRLFRCR
160 170 180 190 200
FNTSKSLRRQ SSGNKLVLIR GRFHAHPPGA YWAGNPVFTA FCAPLEPRPR
210 220 230 240 250
PGPGPGPGPG PASLFLAMFQ SRHAKDLALL DVSESVLIYL GFERSELLCK
260 270 280 290 300
SWYGLLHPED LAQASSQHYR LLAESGDIQA EMVVRLQAKH GGWTWIYCML
310 320 330 340 350
YSEGPEGPFT ANNYPISDTE AWSLRQQLNS EDTQAAYVLG TPAVLPSFSE
360 370 380 390 400
NVFSQEQCSN PLFTPSLGTP RSASFPRAPE LGVISTPEEL PQPSKELDFS
410 420 430 440 450
YLPFPARPEP SLQADLSKDL VCTPPYTPHQ PGGCAFLFSL HEPFQTHLPP
460 470 480 490 500
PSSSLQEQLT PSTVTFSEQL TPSSATFPDP LTSSLQGQLT ESSARSFEDQ
510 520 530 540 550
LTPCTSSFPD QLLPSTATFP EPLGSPAHEQ LTPPSTAFQA HLNSPSQTFP
560 570 580 590 600
EQLSPNPTKT YFAQEGCSFL YEKLPPSPSS PGNGDCTLLA LAQLRGPLSV
610 620 630 640 650
DVPLVPEGLL TPEASPVKQS FFHYTEKEQN EIDRLIQQIS QLAQGVDRPF
660 670 680 690 700
SAEAGTGGLE PLGGLEPLNP NLSLSGAGPP VLSLDLKPWK CQELDFLVDP
710 720 730 740 750
DNLFLEETPV EDIFMDLSTP DPNGEWGSGD PEAEVPGGTL SPCNNLSPED
760 770 780 790 800
HSFLEDLATY ETAFETGVST FPYEGFADEL HQLQSQVQDS FHEDGSGGEP
TF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494BA76 | A0A494BA76_MOUSE | Neuronal PAS domain-containing prot... | Npas4 | 236 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB049835 mRNA Translation: BAC19831.1 AB054577 Genomic DNA Translation: BAC53755.1 AY730724 mRNA Translation: AAV28629.1 AK032607 mRNA Translation: BAE20480.1 Frameshift. |
CCDSi | CCDS29445.1 |
RefSeqi | NP_705781.1, NM_153553.5 XP_006531783.1, XM_006531720.3 XP_006531784.1, XM_006531721.3 XP_011246921.1, XM_011248619.2 |
Genome annotation databases
Ensembli | ENSMUST00000056129; ENSMUSP00000062992; ENSMUSG00000045903 |
GeneIDi | 225872 |
KEGGi | mmu:225872 |
UCSCi | uc008gbu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB049835 mRNA Translation: BAC19831.1 AB054577 Genomic DNA Translation: BAC53755.1 AY730724 mRNA Translation: AAV28629.1 AK032607 mRNA Translation: BAE20480.1 Frameshift. |
CCDSi | CCDS29445.1 |
RefSeqi | NP_705781.1, NM_153553.5 XP_006531783.1, XM_006531720.3 XP_006531784.1, XM_006531721.3 XP_011246921.1, XM_011248619.2 |
3D structure databases
AlphaFoldDBi | Q8BGD7 |
SMRi | Q8BGD7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230436, 2 interactors |
STRINGi | 10090.ENSMUSP00000062992 |
PTM databases
PhosphoSitePlusi | Q8BGD7 |
Proteomic databases
PaxDbi | Q8BGD7 |
PRIDEi | Q8BGD7 |
ProteomicsDBi | 293945 |
Protocols and materials databases
ABCDi | Q8BGD7, 3 sequenced antibodies |
Antibodypediai | 30154, 205 antibodies from 30 providers |
DNASUi | 225872 |
Genome annotation databases
Ensembli | ENSMUST00000056129; ENSMUSP00000062992; ENSMUSG00000045903 |
GeneIDi | 225872 |
KEGGi | mmu:225872 |
UCSCi | uc008gbu.2, mouse |
Organism-specific databases
CTDi | 266743 |
MGIi | MGI:2664186, Npas4 |
VEuPathDBi | HostDB:ENSMUSG00000045903 |
Phylogenomic databases
eggNOGi | ENOG502QRXX, Eukaryota |
GeneTreei | ENSGT00530000064165 |
HOGENOMi | CLU_013890_0_0_1 |
InParanoidi | Q8BGD7 |
OMAi | KTYFTQE |
OrthoDBi | 219290at2759 |
PhylomeDBi | Q8BGD7 |
TreeFami | TF319684 |
Miscellaneous databases
BioGRID-ORCSi | 225872, 1 hit in 75 CRISPR screens |
ChiTaRSi | Npas4, mouse |
PROi | PR:Q8BGD7 |
RNActi | Q8BGD7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000045903, Expressed in islet of Langerhans and 81 other tissues |
ExpressionAtlasi | Q8BGD7, baseline and differential |
Genevisiblei | Q8BGD7, MM |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00091, PAS, 2 hits |
SUPFAMi | SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NPAS4_MOUSE | |
Accessioni | Q8BGD7Primary (citable) accession number: Q8BGD7 Secondary accession number(s): Q3V3U3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot