Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA wybutosine-synthesizing protein 2 homolog

Gene

Trmt12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei215S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei255S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayi
UPA00375

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 2 homolog (EC:2.5.1.114)
Short name:
tRNA-yW-synthesizing protein 2
Alternative name(s):
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
Gene namesi
Name:Trmt12
Synonyms:Tyw2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1915510 Trmt12

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818381 – 446tRNA wybutosine-synthesizing protein 2 homologAdd BLAST446

Proteomic databases

MaxQBiQ8BG71
PaxDbiQ8BG71
PeptideAtlasiQ8BG71
PRIDEiQ8BG71

PTM databases

iPTMnetiQ8BG71
PhosphoSitePlusiQ8BG71

Expressioni

Gene expression databases

BgeeiENSMUSG00000037085 Expressed in 54 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_TRMT12
GenevisibleiQ8BG71 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047831

Structurei

3D structure databases

ProteinModelPortaliQ8BG71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni283 – 284S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1227 Eukaryota
COG2520 LUCA
GeneTreeiENSGT00390000006078
HOGENOMiHOG000294185
HOVERGENiHBG108624
InParanoidiQ8BG71
KOiK07055
OMAiWQRHGNL
OrthoDBiEOG091G06O6
PhylomeDBiQ8BG71
TreeFamiTF314137

Family and domain databases

InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BG71-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERECEESVV VAVVTEPRFT QRYRDYLEEQ KLLDRLHRVA KLRDGAVALP
60 70 80 90 100
VLAESLSEQH LQELRDRVAP GSTCVLTRLP DPLPSKKARV RSPAQILCLE
110 120 130 140 150
VRRWVEDRGV TWSAELEADL PRSWQRHGDL MLLSEDCFQA TLWKGLEPEL
160 170 180 190 200
WETVASALGV QRLAKRGRVL PDGTRTPSVT LLLGDHGWVE HMDNGIRYKF
210 220 230 240 250
DVTQCMFSFG NITEKLRVAS LSCAGEVLVD LYAGIGYFTL PFLVHAGAAF
260 270 280 290 300
VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS DIADRVNLGL
310 320 330 340 350
IPSSKEGWPV ACQVLRKDVG GILHIHQNVE SFSGKTPQPP GSNNVEKEHW
360 370 380 390 400
PRPQKITTDT QGNGTTENFR GEISSANKPE WWRWAESAET QIASLLHQVH
410 420 430 440
GKPWRTRILH VHPVKSYAPH VDHIVLDLEC RPLTSSWPEG VDLLTQ
Length:446
Mass (Da):50,299
Last modified:March 1, 2003 - v1
Checksum:iBB416D36D212E44B
GO

Sequence cautioni

The sequence AAH52533 differs from that shown. Reason: Frameshift at position 211.Curated
The sequence BAB31750 differs from that shown. Reason: Frameshift at position 420.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti396L → F in AAH52533 (PubMed:15489334).Curated1
Sequence conflicti425V → I in BAB31750 (PubMed:16141072).Curated1
Sequence conflicti431R → Q in AAH52533 (PubMed:15489334).Curated1
Sequence conflicti431R → Q in AAH55850 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019481 mRNA Translation: BAB31750.1 Frameshift.
AK031429 mRNA Translation: BAC27399.1
AK076857 mRNA Translation: BAC36508.1
AK079291 mRNA Translation: BAC37597.1
BC052533 mRNA Translation: AAH52533.1 Frameshift.
BC055850 mRNA Translation: AAH55850.1
CCDSiCCDS37081.1
RefSeqiNP_080918.2, NM_026642.2
UniGeneiMm.278805

Genome annotation databases

EnsembliENSMUST00000036937; ENSMUSP00000047831; ENSMUSG00000037085
GeneIDi68260
KEGGimmu:68260
UCSCiuc007vtp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019481 mRNA Translation: BAB31750.1 Frameshift.
AK031429 mRNA Translation: BAC27399.1
AK076857 mRNA Translation: BAC36508.1
AK079291 mRNA Translation: BAC37597.1
BC052533 mRNA Translation: AAH52533.1 Frameshift.
BC055850 mRNA Translation: AAH55850.1
CCDSiCCDS37081.1
RefSeqiNP_080918.2, NM_026642.2
UniGeneiMm.278805

3D structure databases

ProteinModelPortaliQ8BG71
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047831

PTM databases

iPTMnetiQ8BG71
PhosphoSitePlusiQ8BG71

Proteomic databases

MaxQBiQ8BG71
PaxDbiQ8BG71
PeptideAtlasiQ8BG71
PRIDEiQ8BG71

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036937; ENSMUSP00000047831; ENSMUSG00000037085
GeneIDi68260
KEGGimmu:68260
UCSCiuc007vtp.1 mouse

Organism-specific databases

CTDi55039
MGIiMGI:1915510 Trmt12

Phylogenomic databases

eggNOGiKOG1227 Eukaryota
COG2520 LUCA
GeneTreeiENSGT00390000006078
HOGENOMiHOG000294185
HOVERGENiHBG108624
InParanoidiQ8BG71
KOiK07055
OMAiWQRHGNL
OrthoDBiEOG091G06O6
PhylomeDBiQ8BG71
TreeFamiTF314137

Enzyme and pathway databases

UniPathwayi
UPA00375

Miscellaneous databases

PROiPR:Q8BG71
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037085 Expressed in 54 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_TRMT12
GenevisibleiQ8BG71 MM

Family and domain databases

InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYW2_MOUSE
AccessioniPrimary (citable) accession number: Q8BG71
Secondary accession number(s): Q0P5W5, Q7TNQ8, Q9D2N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again