UniProtKB - Q8BG60 (TXNIP_MOUSE)
Thioredoxin-interacting protein
Txnip
Functioni
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity).
Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.
By similarity2 PublicationsGO - Molecular functioni
- enzyme inhibitor activity Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cellular response to tumor cell Source: UniProtKB
- keratinocyte differentiation Source: MGI
- negative regulation of cell division Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- platelet-derived growth factor receptor signaling pathway Source: MGI
- positive regulation of apoptotic process Source: MGI
- protein import into nucleus Source: MGI
- protein transport Source: GO_Central
- regulation of cell population proliferation Source: MGI
- response to calcium ion Source: Ensembl
- response to estradiol Source: Ensembl
- response to glucose Source: Ensembl
- response to hydrogen peroxide Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- response to oxidative stress Source: MGI
- response to progesterone Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
Keywordsi
Biological process | Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-844456, The NLRP3 inflammasome |
Names & Taxonomyi
Protein namesi | Recommended name: Thioredoxin-interacting proteinAlternative name(s): Vitamin D3 up-regulated protein 1 |
Gene namesi | Name:Txnip Synonyms:Vdup1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1889549, Txnip |
VEuPathDBi | HostDB:ENSMUSG00000038393 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial intermembrane space Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Keywords - Diseasei
Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000250490 | 1 – 397 | Thioredoxin-interacting proteinAdd BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 63 | InterchainBy similarity | ||
Cross-linki | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 362 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8BG60 |
MaxQBi | Q8BG60 |
PaxDbi | Q8BG60 |
PeptideAtlasi | Q8BG60 |
PRIDEi | Q8BG60 |
ProteomicsDBi | 298044 [Q8BG60-1] 298045 [Q8BG60-2] |
PTM databases
iPTMneti | Q8BG60 |
PhosphoSitePlusi | Q8BG60 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000038393, Expressed in islet of Langerhans and 333 other tissues |
Genevisiblei | Q8BG60, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
Interacts with TXN/thioredoxin through its redox-active site.
Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1.
Interacts (via C-terminus) with ITCH (via WW domains).
Interacts with DDIT4 (By similarity).
By similarityGO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207912, 1 interactor |
CORUMi | Q8BG60 |
IntActi | Q8BG60, 2 interactors |
MINTi | Q8BG60 |
STRINGi | 10090.ENSMUSP00000102710 |
Miscellaneous databases
RNActi | Q8BG60, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3780, Eukaryota |
GeneTreei | ENSGT00940000158522 |
HOGENOMi | CLU_039221_1_1_1 |
InParanoidi | Q8BG60 |
OMAi | MVMFKKV |
OrthoDBi | 817924at2759 |
PhylomeDBi | Q8BG60 |
TreeFami | TF313650 |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin-like_C IPR011021, Arrestin-like_N IPR011022, Arrestin_C-like IPR014756, Ig_E-set |
Pfami | View protein in Pfam PF02752, Arrestin_C, 1 hit PF00339, Arrestin_N, 1 hit |
SMARTi | View protein in SMART SM01017, Arrestin_C, 1 hit |
SUPFAMi | SSF81296, SSF81296, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVMFKKIKSF EVVFNDPEKV YGSGEKVAGR VIVEVCEVTR VKAVRILACG
60 70 80 90 100
VAKVLWMQGS QQCKQTLDYL RYEDTLLLEE QPTAGENEMV IMRPGNKYEY
110 120 130 140 150
KFGFELPQGP LGTSFKGKYG CVDYWVKAFL DRPSQPTQEA KKNFEVMDLV
160 170 180 190 200
DVNTPDLMAP VSAKKEKKVS CMFIPDGRVS VSARIDRKGF CEGDDISIHA
210 220 230 240 250
DFENTCSRIV VPKAAIVARH TYLANGQTKV FTQKLSSVRG NHIISGTCAS
260 270 280 290 300
WRGKSLRVQK IRPSILGCNI LKVEYSLLIY VSVPGSKKVI LDLPLVIGSR
310 320 330 340 350
SGLSSRTSSM ASRTSSEMSW IDLNIPDTPE APPCYMDIIP EDHRLESPTT
360 370 380 390
PLLDDVDDSQ DSPIFMYAPE FQFMPPPTYT EVDPCVLNNN NNNNNVQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | E → G in BAB23444 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 165 | K → E in AAD48499 (PubMed:10843682).Curated | 1 | |
Sequence conflicti | 175 | P → R in AAD48499 (PubMed:10843682).Curated | 1 | |
Sequence conflicti | 308 | S → F in AAG32665 (PubMed:11376942).Curated | 1 | |
Sequence conflicti | 308 | S → F in AAG32666 (PubMed:11376942).Curated | 1 | |
Sequence conflicti | 395 | N → NN in AAH31850 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 395 | Missing in AAD48499 (PubMed:10843682).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020653 | 84 | Missing in isoform 2. 4 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173681 mRNA Translation: AAD48499.1 AF282825 Genomic DNA Translation: AAG32665.1 AF282826 mRNA Translation: AAG32666.1 AK004653 mRNA Translation: BAB23444.1 AK082476 mRNA Translation: BAC38504.1 AK088870 mRNA Translation: BAC40625.1 AK089304 mRNA Translation: BAC40834.1 AK089351 mRNA Translation: BAC40850.1 AK089403 mRNA Translation: BAC40868.1 AK155517 mRNA Translation: BAE33304.1 AK171205 mRNA Translation: BAE42311.1 AK171312 mRNA Translation: BAE42386.1 AK171425 mRNA Translation: BAE42444.1 AK171456 mRNA Translation: BAE42465.1 AK171464 mRNA Translation: BAE42470.1 AK171527 mRNA Translation: BAE42508.1 BC031850 mRNA Translation: AAH31850.1 |
CCDSi | CCDS17640.1 [Q8BG60-2] CCDS38555.1 [Q8BG60-1] |
RefSeqi | NP_001009935.1, NM_001009935.2 [Q8BG60-1] NP_076208.2, NM_023719.2 [Q8BG60-2] |
Genome annotation databases
Ensembli | ENSMUST00000049093; ENSMUSP00000041467; ENSMUSG00000038393 [Q8BG60-2] ENSMUST00000074519; ENSMUSP00000102710; ENSMUSG00000038393 [Q8BG60-1] |
GeneIDi | 56338 |
KEGGi | mmu:56338 |
UCSCi | uc008qnc.2, mouse [Q8BG60-1] uc008qnd.2, mouse [Q8BG60-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173681 mRNA Translation: AAD48499.1 AF282825 Genomic DNA Translation: AAG32665.1 AF282826 mRNA Translation: AAG32666.1 AK004653 mRNA Translation: BAB23444.1 AK082476 mRNA Translation: BAC38504.1 AK088870 mRNA Translation: BAC40625.1 AK089304 mRNA Translation: BAC40834.1 AK089351 mRNA Translation: BAC40850.1 AK089403 mRNA Translation: BAC40868.1 AK155517 mRNA Translation: BAE33304.1 AK171205 mRNA Translation: BAE42311.1 AK171312 mRNA Translation: BAE42386.1 AK171425 mRNA Translation: BAE42444.1 AK171456 mRNA Translation: BAE42465.1 AK171464 mRNA Translation: BAE42470.1 AK171527 mRNA Translation: BAE42508.1 BC031850 mRNA Translation: AAH31850.1 |
CCDSi | CCDS17640.1 [Q8BG60-2] CCDS38555.1 [Q8BG60-1] |
RefSeqi | NP_001009935.1, NM_001009935.2 [Q8BG60-1] NP_076208.2, NM_023719.2 [Q8BG60-2] |
3D structure databases
AlphaFoldDBi | Q8BG60 |
SMRi | Q8BG60 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207912, 1 interactor |
CORUMi | Q8BG60 |
IntActi | Q8BG60, 2 interactors |
MINTi | Q8BG60 |
STRINGi | 10090.ENSMUSP00000102710 |
PTM databases
iPTMneti | Q8BG60 |
PhosphoSitePlusi | Q8BG60 |
Proteomic databases
EPDi | Q8BG60 |
MaxQBi | Q8BG60 |
PaxDbi | Q8BG60 |
PeptideAtlasi | Q8BG60 |
PRIDEi | Q8BG60 |
ProteomicsDBi | 298044 [Q8BG60-1] 298045 [Q8BG60-2] |
Protocols and materials databases
Antibodypediai | 73170, 370 antibodies from 35 providers |
DNASUi | 56338 |
Genome annotation databases
Ensembli | ENSMUST00000049093; ENSMUSP00000041467; ENSMUSG00000038393 [Q8BG60-2] ENSMUST00000074519; ENSMUSP00000102710; ENSMUSG00000038393 [Q8BG60-1] |
GeneIDi | 56338 |
KEGGi | mmu:56338 |
UCSCi | uc008qnc.2, mouse [Q8BG60-1] uc008qnd.2, mouse [Q8BG60-2] |
Organism-specific databases
CTDi | 10628 |
MGIi | MGI:1889549, Txnip |
VEuPathDBi | HostDB:ENSMUSG00000038393 |
Phylogenomic databases
eggNOGi | KOG3780, Eukaryota |
GeneTreei | ENSGT00940000158522 |
HOGENOMi | CLU_039221_1_1_1 |
InParanoidi | Q8BG60 |
OMAi | MVMFKKV |
OrthoDBi | 817924at2759 |
PhylomeDBi | Q8BG60 |
TreeFami | TF313650 |
Enzyme and pathway databases
Reactomei | R-MMU-844456, The NLRP3 inflammasome |
Miscellaneous databases
BioGRID-ORCSi | 56338, 0 hits in 72 CRISPR screens |
ChiTaRSi | Txnip, mouse |
PROi | PR:Q8BG60 |
RNActi | Q8BG60, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038393, Expressed in islet of Langerhans and 333 other tissues |
Genevisiblei | Q8BG60, MM |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin-like_C IPR011021, Arrestin-like_N IPR011022, Arrestin_C-like IPR014756, Ig_E-set |
Pfami | View protein in Pfam PF02752, Arrestin_C, 1 hit PF00339, Arrestin_N, 1 hit |
SMARTi | View protein in SMART SM01017, Arrestin_C, 1 hit |
SUPFAMi | SSF81296, SSF81296, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | TXNIP_MOUSE | |
Accessioni | Q8BG60Primary (citable) accession number: Q8BG60 Secondary accession number(s): Q8BGQ0 Q9R115 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families