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Protein

Synaptic vesicle glycoprotein 2B

Gene

Sv2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably plays a role in the control of regulated secretion in neural and endocrine cells.1 Publication
(Microbial infection) Receptor for C.botulinum neurotoxin type A (BoNT/A, botA); the toxin probably binds via extracellular loop 4 (PubMed:16543415).1 Publication
(Microbial infection) Possible receptor for C.botulinum neurotoxin type D (BoNT/D, botD) (PubMed:21483489). Not a receptor for C.botulinum neurotoxin type D (BoNT/D, botD) (PubMed:21632541).1 Publication
(Microbial infection) Receptor for C.botulinum neurotoxin type E (BoNT/E); the toxin probably binds via extracellular loop 4 (PubMed:18815274). It probably requires glycosylation of Asn-516 (PubMed:18815274).1 Publication1 Publication

Caution

The use of this protein as a coreceptor for C.botulinum type D (BoNT/D, botD) is controversial. In double SV2A/SV2B knockout mice BoNT/D does not degrade its synaptobrevin target; introducing SV2A, SV2B or SV2C restores target cleavage (PubMed:21483489). However another group does not find a convincing interaction with SV2 (PubMed:21632541).2 Publications

GO - Molecular functioni

GO - Biological processi

  • chemical synaptic transmission Source: MGI
  • neurotransmitter transport Source: UniProtKB-KW

Keywordsi

Molecular functionReceptor
Biological processNeurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicle glycoprotein 2B
Short name:
Synaptic vesicle protein 2B
Gene namesi
Name:Sv2b
Synonyms:Kiaa0735
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1927338 Sv2b

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 110CytoplasmicSequence analysisAdd BLAST110
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 148ExtracellularSequence analysisAdd BLAST17
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 182CytoplasmicSequence analysisAdd BLAST13
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 205ExtracellularSequence analysis2
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Topological domaini227 – 237CytoplasmicSequence analysisAdd BLAST11
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 277ExtracellularSequence analysisAdd BLAST19
Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
Topological domaini299 – 390CytoplasmicSequence analysisAdd BLAST92
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Topological domaini412 – 535ExtracellularSequence analysisAdd BLAST124
Transmembranei536 – 556HelicalSequence analysisAdd BLAST21
Topological domaini557 – 565CytoplasmicSequence analysis9
Transmembranei566 – 586HelicalSequence analysisAdd BLAST21
Topological domaini587 – 592ExtracellularSequence analysis6
Transmembranei593 – 613HelicalSequence analysisAdd BLAST21
Topological domaini614 – 626CytoplasmicSequence analysisAdd BLAST13
Transmembranei627 – 649HelicalSequence analysisAdd BLAST23
Topological domaini650 – 653ExtracellularSequence analysis4
Transmembranei654 – 672HelicalSequence analysisAdd BLAST19
Topological domaini673 – 683CytoplasmicSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice display no particular phenotype (PubMed:10624962). Single knockout mice survive significantly longer than wild-type mice upon exposure to C.botulinum neurotoxin type A (BoNT/A, botA) (PubMed:16543415). Mice lacking both Sv2a and Sv2b experience severe epileptic seizures and die immediately or shortly after birth similarly to mice lacking only Sv2a (PubMed:10624962). Single knockout mice bind reduced amounts of BoNT/A than wild-type mice (PubMed:16543415). Single knockout mice are significantly more resistant to C.botulinum neurotoxin type E (BoNT/E) than wild-type mice (PubMed:18815274). In single knockout mice, synaptobrevin (VAMP, the target of C.botulinum neurotoxin type D, BoNT/D) is degraded by BoNT/D, and hippocampal neurons bind BoNT/D (PubMed:21483489). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind BoNT/A, nor do they take it up (PubMed:16543415, PubMed:18815274). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind C.botulinum neurotoxin type E (BoNT/E), nor do they take it up (PubMed:18815274). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind C.botulinum neurotoxin type D (BoNT/D, botD), nor do they take it up (PubMed:21483489). Hippocampal neurons from young mice lacking both Sv2a and Sv2b take up C.botulinum neurotoxin type C (BoNT/C) and C.botulinum neurotoxin type F (BonT/F, botF) normally (PubMed:21483489).5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397691 – 683Synaptic vesicle glycoprotein 2BAdd BLAST683

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineCombined sources1
Modified residuei36PhosphothreonineCombined sources1
Modified residuei423PhosphotyrosineCombined sources1
Glycosylationi441N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi491N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi516N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication
The N-terminal cytoplasmic domain is phosphorylated by CK1.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BG39
PaxDbiQ8BG39
PeptideAtlasiQ8BG39
PRIDEiQ8BG39

PTM databases

iPTMnetiQ8BG39
PhosphoSitePlusiQ8BG39
SwissPalmiQ8BG39

Expressioni

Tissue specificityi

Expressed in ribbon synapses of the retina (at protein level) (PubMed:12687700). Expressed in diaphragm motor nerve terminals (at protein level) (PubMed:16543415). Expressed in hippocampus neurons (at protein level) (PubMed:18815274).3 Publications

Developmental stagei

Expressed during synaptogenesis in the retina (at protein level).1 Publication

Inductioni

Up-regulated upon Sv2a depletion.1 Publication

Gene expression databases

BgeeiENSMUSG00000053025 Expressed in 189 organ(s), highest expression level in brain
CleanExiMM_SV2B
ExpressionAtlasiQ8BG39 baseline and differential
GenevisibleiQ8BG39 MM

Interactioni

Subunit structurei

Interacts with SYT1 in a calcium-independent manner. Forms a complex with SYT1, syntaxin-1 and SNAP25.1 Publication
(Microbial infection) Interacts with C.botulinum neurotoxin type A (BoNT/A, botA).1 Publication1 Publication
(Microbial infection) Interacts with C.botulinum neurotoxin type D (BoNT/D, botD) (PubMed:21483489). No evidence for its interaction with BoNT/D has also been published (PubMed:21632541).2 Publications

Protein-protein interaction databases

BioGridi211036, 1 interactor
STRINGi10090.ENSMUSP00000082254

Structurei

3D structure databases

ProteinModelPortaliQ8BG39
SMRiQ8BG39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRID Eukaryota
ENOG410YQME LUCA
GeneTreeiENSGT00550000074384
HOGENOMiHOG000065727
HOVERGENiHBG053967
InParanoidiQ8BG39
KOiK06258
OMAiIDECGHG
OrthoDBiEOG091G02NC
PhylomeDBiQ8BG39
TreeFamiTF324824

Family and domain databases

CDDicd06174 MFS, 2 hits
InterProiView protein in InterPro
IPR001646 5peptide_repeat
IPR011701 MFS
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
IPR022308 SV2
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
PF13599 Pentapeptide_4, 1 hit
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR01299 synapt_SV2, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8BG39-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDYRYRDNY EGYAPSDGYY RSNEQNQEED AQSDVTEGHD EEDEIYEGEY
60 70 80 90 100
QGIPHPDDVK SKQTKMAPSR ADGLGGQADL MAERMEDEEE LAHQYETIID
110 120 130 140 150
ECGHGRFQWT LFFVLGLALM ADGVEIFVVS FALPSAEKDM CLSSSKKGML
160 170 180 190 200
GLIVYLGMMA GAFILGGLAD KLGRKKVLSM SLAINASFAS LSSFVQGYGA
210 220 230 240 250
FLFCRLISGI GIGGSLPIVF AYFSEFLSRE KRGEHLSWLG IFWMTGGIYA
260 270 280 290 300
SAMAWSIIPH YGWGFSMGTN YHFHSWRVFV IVCALPATVS MVALKFMPES
310 320 330 340 350
PRFLLEMGKH DEAWMILKQV HDTNMRAKGT PEKVFTVSHI KTPKQMDEFI
360 370 380 390 400
EIQSSTGTWY QRWLVRFMTI FKQVWDNALY CVMGPYRMNT LILAVVWFTM
410 420 430 440 450
ALSYYGLTVW FPDMIRYFQD EEYKSKMKVF FGEHVHGATI NFTMENQIHQ
460 470 480 490 500
HGKLVNDKFI KMYFKHVLFE DTFFDKCYFE DVTSTDTYFK NCTIESTTFY
510 520 530 540 550
NTDLYKHKFI NCRFINSTFL EQKEGCHMDF EEDNDFLIYL VSFLGSLSVL
560 570 580 590 600
PGNIISALLM DRIGRLKMIG GSMLISAVCC FFLFFGNSES AMIGWQCLFC
610 620 630 640 650
GTSIAAWNAL DVITVELYPT NQRATAFGIL NGLCKFGAIL GNTIFASFVG
660 670 680
ITKVVPILLA AASLVGGGLI ALRLPETREQ VLM
Length:683
Mass (Da):77,457
Last modified:March 1, 2003 - v1
Checksum:iCBDC19B281B9E4F3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RQ76A0A0U1RQ76_MOUSE
Synaptic vesicle glycoprotein 2B
Sv2b
9Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18G → D in AAG28491 (PubMed:10624962).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029736 mRNA Translation: BAC26590.1
AK043830 mRNA Translation: BAC31669.1
AK139478 mRNA Translation: BAE24029.1
AK140104 mRNA Translation: BAE24238.1
AK147261 mRNA Translation: BAE27804.1
BC060224 mRNA Translation: AAH60224.1
AF196782 Genomic DNA Translation: AAG28491.1
AK122359 mRNA Translation: BAC65641.1
CCDSiCCDS21368.1
RefSeqiNP_001103223.1, NM_001109753.1
NP_705807.2, NM_153579.4
XP_006541122.1, XM_006541059.3
XP_006541123.1, XM_006541060.3
XP_006541124.1, XM_006541061.2
XP_017167699.1, XM_017312210.1
XP_017167701.1, XM_017312212.1
UniGeneiMm.273082

Genome annotation databases

EnsembliENSMUST00000085164; ENSMUSP00000082254; ENSMUSG00000053025
ENSMUST00000165175; ENSMUSP00000127245; ENSMUSG00000053025
ENSMUST00000206344; ENSMUSP00000146049; ENSMUSG00000053025
GeneIDi64176
KEGGimmu:64176
UCSCiuc009hwk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029736 mRNA Translation: BAC26590.1
AK043830 mRNA Translation: BAC31669.1
AK139478 mRNA Translation: BAE24029.1
AK140104 mRNA Translation: BAE24238.1
AK147261 mRNA Translation: BAE27804.1
BC060224 mRNA Translation: AAH60224.1
AF196782 Genomic DNA Translation: AAG28491.1
AK122359 mRNA Translation: BAC65641.1
CCDSiCCDS21368.1
RefSeqiNP_001103223.1, NM_001109753.1
NP_705807.2, NM_153579.4
XP_006541122.1, XM_006541059.3
XP_006541123.1, XM_006541060.3
XP_006541124.1, XM_006541061.2
XP_017167699.1, XM_017312210.1
XP_017167701.1, XM_017312212.1
UniGeneiMm.273082

3D structure databases

ProteinModelPortaliQ8BG39
SMRiQ8BG39
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211036, 1 interactor
STRINGi10090.ENSMUSP00000082254

PTM databases

iPTMnetiQ8BG39
PhosphoSitePlusiQ8BG39
SwissPalmiQ8BG39

Proteomic databases

MaxQBiQ8BG39
PaxDbiQ8BG39
PeptideAtlasiQ8BG39
PRIDEiQ8BG39

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085164; ENSMUSP00000082254; ENSMUSG00000053025
ENSMUST00000165175; ENSMUSP00000127245; ENSMUSG00000053025
ENSMUST00000206344; ENSMUSP00000146049; ENSMUSG00000053025
GeneIDi64176
KEGGimmu:64176
UCSCiuc009hwk.2 mouse

Organism-specific databases

CTDi9899
MGIiMGI:1927338 Sv2b
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IRID Eukaryota
ENOG410YQME LUCA
GeneTreeiENSGT00550000074384
HOGENOMiHOG000065727
HOVERGENiHBG053967
InParanoidiQ8BG39
KOiK06258
OMAiIDECGHG
OrthoDBiEOG091G02NC
PhylomeDBiQ8BG39
TreeFamiTF324824

Miscellaneous databases

PROiPR:Q8BG39
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053025 Expressed in 189 organ(s), highest expression level in brain
CleanExiMM_SV2B
ExpressionAtlasiQ8BG39 baseline and differential
GenevisibleiQ8BG39 MM

Family and domain databases

CDDicd06174 MFS, 2 hits
InterProiView protein in InterPro
IPR001646 5peptide_repeat
IPR011701 MFS
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
IPR022308 SV2
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
PF13599 Pentapeptide_4, 1 hit
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR01299 synapt_SV2, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSV2B_MOUSE
AccessioniPrimary (citable) accession number: Q8BG39
Secondary accession number(s): Q80TT1, Q9ES95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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