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Protein

Sodium-dependent neutral amino acid transporter B(0)AT2

Gene

Slc6a15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent (By similarity).By similarity1 Publication

Kineticsi

  1. KM=195 µM for proline (in the presence of 100 mM NaCL)1 Publication
  2. KM=510 µM for proline (in the presence of 30 mM NaCL)1 Publication
  3. KM=790 µM for proline (in the presence of 3 mM NaCL)1 Publication
  4. KM=81 µM for leucine1 Publication
  5. KM=58 µM for isoleucine1 Publication
  6. KM=40 µM for methionine1 Publication
  7. KM=670 µM for alanine1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Biological processAmino-acid transport, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport
    LigandSodium

    Enzyme and pathway databases

    ReactomeiR-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent neutral amino acid transporter B(0)AT2
    Alternative name(s):
    Sodium- and chloride-dependent neurotransmitter transporter NTT73
    Solute carrier family 6 member 15
    Transporter v7-3
    Gene namesi
    Name:Slc6a15
    Synonyms:B0at2, Ntt73
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 10

    Organism-specific databases

    MGIiMGI:2143484 Slc6a15

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 69CytoplasmicSequence analysisAdd BLAST69
    Transmembranei70 – 90Helical; Name=1Sequence analysisAdd BLAST21
    Transmembranei98 – 117Helical; Name=2Sequence analysisAdd BLAST20
    Transmembranei142 – 162Helical; Name=3Sequence analysisAdd BLAST21
    Topological domaini163 – 225ExtracellularSequence analysisAdd BLAST63
    Transmembranei226 – 244Helical; Name=4Sequence analysisAdd BLAST19
    Transmembranei253 – 270Helical; Name=5Sequence analysisAdd BLAST18
    Transmembranei306 – 323Helical; Name=6Sequence analysisAdd BLAST18
    Transmembranei335 – 356Helical; Name=7Sequence analysisAdd BLAST22
    Topological domaini357 – 452ExtracellularSequence analysisAdd BLAST96
    Transmembranei453 – 472Helical; Name=8Sequence analysisAdd BLAST20
    Transmembranei496 – 514Helical; Name=9Sequence analysisAdd BLAST19
    Transmembranei530 – 550Helical; Name=10Sequence analysisAdd BLAST21
    Transmembranei571 – 592Helical; Name=11Sequence analysisAdd BLAST22
    Transmembranei620 – 642Helical; Name=12Sequence analysisAdd BLAST23
    Topological domaini643 – 729CytoplasmicSequence analysisAdd BLAST87

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and fertile and fail to demonstrate any striking abnormality in motor and sensory functions. Other transporters could help to compensate for lost of SLC6A15.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002147991 – 729Sodium-dependent neutral amino acid transporter B(0)AT2Add BLAST729

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei25PhosphoserineBy similarity1
    Modified residuei55PhosphoserineBy similarity1
    Glycosylationi187N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi394N-linked (GlcNAc...) asparagineSequence analysis1
    Modified residuei687PhosphoserineBy similarity1
    Modified residuei699PhosphoserineBy similarity1
    Modified residuei701PhosphoserineBy similarity1

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BG16
    PaxDbiQ8BG16
    PeptideAtlasiQ8BG16
    PRIDEiQ8BG16

    PTM databases

    iPTMnetiQ8BG16
    PhosphoSitePlusiQ8BG16
    SwissPalmiQ8BG16

    Expressioni

    Tissue specificityi

    Significant expressed in brain, lung and kidney. regions, the cortex, the cerebellum and the brain stem.1 Publication

    Developmental stagei

    Detected throughout development, starting with the pre-implantation embryo.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000019894 Expressed in 209 organ(s), highest expression level in saccule of membranous labyrinth
    ExpressionAtlasiQ8BG16 baseline and differential
    GenevisibleiQ8BG16 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000073829

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BG16
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3659 Eukaryota
    COG0733 LUCA
    GeneTreeiENSGT00760000119044
    HOGENOMiHOG000116406
    HOVERGENiHBG071421
    InParanoidiQ8BG16
    KOiK05048
    OMAiIPHHINF
    OrthoDBiEOG091G08PX
    PhylomeDBiQ8BG16
    TreeFamiTF352709

    Family and domain databases

    InterProiView protein in InterPro
    IPR000175 Na/ntran_symport
    IPR002438 Na/ntran_symport_orphan
    IPR037272 SNS_sf
    PANTHERiPTHR11616 PTHR11616, 1 hit
    PfamiView protein in Pfam
    PF00209 SNF, 1 hit
    PRINTSiPR00176 NANEUSMPORT
    PR01206 ORPHTRNSPORT
    SUPFAMiSSF161070 SSF161070, 1 hit
    PROSITEiView protein in PROSITE
    PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
    PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
    PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

    Q8BG16-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EEVSKKSELI VDVQEEKDTD
    60 70 80 90 100
    AEDGSEADDE RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL
    110 120 130 140 150
    LPYLILLLVI GIPLFFLELS VGQRIRRGSI GVWNYISPKL GGIGFASCVV
    160 170 180 190 200
    CYFVALYYNV IIGWTLFYFS QSFQQPLPWD QCPLVKNASH TYVEPECEQS
    210 220 230 240 250
    SATTYYWYRE ALDITSSISD SGGLNWKMTV CLLVAWVMVC LAMIKGIQSS
    260 270 280 290 300
    GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR
    310 320 330 340 350
    EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL
    360 370 380 390 400
    VVFAVLGFKA NIVNEKCISQ NSEMILKLLK MGNISWDVIP HHINLSAVTV
    410 420 430 440 450
    EDYRLVYDII QKVKEEEFAV LHLNACQIED ELNKAVQGTG LAFIAFTEAM
    460 470 480 490 500
    THFPASPFWS VMFFLMLINL GLGSMFGTIE GIITPIVDTF KVRKEILTVI
    510 520 530 540 550
    CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE NIAVSFVYGI
    560 570 580 590 600
    DKFIEDLTDM LGFAPSKYYY YMWKYISPLM LLTLLIASIV NMGLSPPGYN
    610 620 630 640 650
    AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFIIR RCNLIDDSSG
    660 670 680 690 700
    NLASVTYKRG RVLKEPVNLE GDDASLIHGK IPSEMSSPNF GKNIYRKQSG
    710 720
    SPTLDTAPNG RYGIGYLMAD MPDMPESDL
    Length:729
    Mass (Da):81,792
    Last modified:March 1, 2003 - v1
    Checksum:iD336735E9D1E9B97
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A1W2P744A0A1W2P744_MOUSE
    Transporter
    Slc6a15
    127Annotation score:

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY149280 mRNA Translation: AAN75437.1
    AY149281 mRNA Translation: AAN75438.1
    AY149282 Genomic DNA Translation: AAN75439.1
    AM085111 mRNA Translation: CAJ29896.1
    AK036136 mRNA Translation: BAC29315.1
    CH466539 Genomic DNA Translation: EDL21676.1
    CH466539 Genomic DNA Translation: EDL21677.1
    BC076593 mRNA Translation: AAH76593.1
    CCDSiCCDS24156.1
    RefSeqiNP_001239259.1, NM_001252330.1
    NP_780537.1, NM_175328.3
    XP_006513045.1, XM_006512982.3
    XP_006513046.1, XM_006512983.3
    XP_006513047.1, XM_006512984.3
    UniGeneiMm.458408
    Mm.489818

    Genome annotation databases

    EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894
    ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894
    GeneIDi103098
    KEGGimmu:103098
    UCSCiuc007gyj.2 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY149280 mRNA Translation: AAN75437.1
    AY149281 mRNA Translation: AAN75438.1
    AY149282 Genomic DNA Translation: AAN75439.1
    AM085111 mRNA Translation: CAJ29896.1
    AK036136 mRNA Translation: BAC29315.1
    CH466539 Genomic DNA Translation: EDL21676.1
    CH466539 Genomic DNA Translation: EDL21677.1
    BC076593 mRNA Translation: AAH76593.1
    CCDSiCCDS24156.1
    RefSeqiNP_001239259.1, NM_001252330.1
    NP_780537.1, NM_175328.3
    XP_006513045.1, XM_006512982.3
    XP_006513046.1, XM_006512983.3
    XP_006513047.1, XM_006512984.3
    UniGeneiMm.458408
    Mm.489818

    3D structure databases

    ProteinModelPortaliQ8BG16
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000073829

    PTM databases

    iPTMnetiQ8BG16
    PhosphoSitePlusiQ8BG16
    SwissPalmiQ8BG16

    Proteomic databases

    MaxQBiQ8BG16
    PaxDbiQ8BG16
    PeptideAtlasiQ8BG16
    PRIDEiQ8BG16

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894
    ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894
    GeneIDi103098
    KEGGimmu:103098
    UCSCiuc007gyj.2 mouse

    Organism-specific databases

    CTDi55117
    MGIiMGI:2143484 Slc6a15

    Phylogenomic databases

    eggNOGiKOG3659 Eukaryota
    COG0733 LUCA
    GeneTreeiENSGT00760000119044
    HOGENOMiHOG000116406
    HOVERGENiHBG071421
    InParanoidiQ8BG16
    KOiK05048
    OMAiIPHHINF
    OrthoDBiEOG091G08PX
    PhylomeDBiQ8BG16
    TreeFamiTF352709

    Enzyme and pathway databases

    ReactomeiR-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

    Miscellaneous databases

    ChiTaRSiSlc6a15 mouse
    PROiPR:Q8BG16
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000019894 Expressed in 209 organ(s), highest expression level in saccule of membranous labyrinth
    ExpressionAtlasiQ8BG16 baseline and differential
    GenevisibleiQ8BG16 MM

    Family and domain databases

    InterProiView protein in InterPro
    IPR000175 Na/ntran_symport
    IPR002438 Na/ntran_symport_orphan
    IPR037272 SNS_sf
    PANTHERiPTHR11616 PTHR11616, 1 hit
    PfamiView protein in Pfam
    PF00209 SNF, 1 hit
    PRINTSiPR00176 NANEUSMPORT
    PR01206 ORPHTRNSPORT
    SUPFAMiSSF161070 SSF161070, 1 hit
    PROSITEiView protein in PROSITE
    PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
    PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
    PS50267 NA_NEUROTRAN_SYMP_3, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS6A15_MOUSE
    AccessioniPrimary (citable) accession number: Q8BG16
    Secondary accession number(s): Q333Y0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: March 1, 2003
    Last modified: November 7, 2018
    This is version 115 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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