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Protein

Peflin

Gene

Pef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (By similarity). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi118 – 1291PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi185 – 1962PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PeflinBy similarity
Alternative name(s):
PEF protein with a long N-terminal hydrophobic domainBy similarity
Penta-EF hand domain-containing protein 1Imported
Gene namesi
Name:Pef1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915148 Pef1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470461 – 275PeflinAdd BLAST275

Post-translational modificationi

Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ8BFY6
MaxQBiQ8BFY6
PaxDbiQ8BFY6
PeptideAtlasiQ8BFY6
PRIDEiQ8BFY6

PTM databases

PhosphoSitePlusiQ8BFY6
SwissPalmiQ8BFY6

Expressioni

Gene expression databases

BgeeiENSMUSG00000028779 Expressed in 280 organ(s), highest expression level in colon
CleanExiMM_PEF1
GenevisibleiQ8BFY6 MM

Interactioni

Subunit structurei

Heterodimer; heterodimerizes (via the EF-hand 5) with PDCD6. Dissociates from PDCD6 in presence of calcium.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030563

Structurei

3D structure databases

ProteinModelPortaliQ8BFY6
SMRiQ8BFY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 2919
Repeati31 – 3929
Repeati41 – 4939
Repeati50 – 59410
Repeati60 – 6859
Repeati76 – 8469
Repeati85 – 9177
Repeati92 – 10089
Domaini105 – 140EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini146 – 174EF-hand 2PROSITE-ProRule annotationAdd BLAST29
Domaini172 – 207EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini208 – 244EF-hand 4PROSITE-ProRule annotationAdd BLAST37
Domaini245 – 274EF-hand 5PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 1008 X 9 AA approximate tandem repeat of [AP]-P-G-G-P-Y-G-G-P-PAdd BLAST80
Regioni195 – 275Required for interaction with PDCD6By similarityAdd BLAST81

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037 Eukaryota
ENOG410YKQK LUCA
GeneTreeiENSGT00620000087734
HOGENOMiHOG000231983
HOVERGENiHBG004492
InParanoidiQ8BFY6
OMAiPPQNYGF
OrthoDBiEOG091G0ISY
PhylomeDBiQ8BFY6
TreeFamiTF314682

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q8BFY6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASYPNGQSC PGAAGQVPGV PPGGYYPGPP HGGGQYGSGL PPGGGYGAPA
60 70 80 90 100
PGGPYGYPSA GGVPSGTPSG PYGGIPPGGP YGQLPPGGPY GTQPGHYGQG
110 120 130 140 150
GVPPNVDPEA YSWFQSVDAD HSGYISLKEL KQALVNSNWS SFNDETCLMM
160 170 180 190 200
INMFDKTKSG RIDVAGFSAL WKFLQQWRNL FQQYDRDRSG SISSTELQQA
210 220 230 240 250
LSQMGYNLSP QFTQLLVSRY CARSAIPAMQ LDCFIKVCTQ LQVLTEAFRE
260 270
KDTAVQGNIR LSFEDFVTMT ASRML
Length:275
Mass (Da):29,228
Last modified:March 1, 2003 - v1
Checksum:iDE4782B47ABEE9E2
GO

Sequence cautioni

The sequence BAB28735 differs from that shown. Reason: Frameshift at position 248.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148L → H in AAH19191 (PubMed:15489334).Curated1
Sequence conflicti218S → F in BAB28735 (PubMed:16141072).Curated1
Sequence conflicti257G → R in BAB25010 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007394 mRNA Translation: BAB25010.1
AK013238 mRNA Translation: BAB28735.1 Frameshift.
AK046247 mRNA Translation: BAC32654.1
AK075978 mRNA Translation: BAC36091.1
BC019191 mRNA Translation: AAH19191.1
CCDSiCCDS18706.1
RefSeqiNP_080717.2, NM_026441.4
UniGeneiMm.20818

Genome annotation databases

EnsembliENSMUST00000030563; ENSMUSP00000030563; ENSMUSG00000028779
ENSMUST00000118199; ENSMUSP00000112638; ENSMUSG00000028779
GeneIDi67898
KEGGimmu:67898
UCSCiuc008uyw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007394 mRNA Translation: BAB25010.1
AK013238 mRNA Translation: BAB28735.1 Frameshift.
AK046247 mRNA Translation: BAC32654.1
AK075978 mRNA Translation: BAC36091.1
BC019191 mRNA Translation: AAH19191.1
CCDSiCCDS18706.1
RefSeqiNP_080717.2, NM_026441.4
UniGeneiMm.20818

3D structure databases

ProteinModelPortaliQ8BFY6
SMRiQ8BFY6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030563

PTM databases

PhosphoSitePlusiQ8BFY6
SwissPalmiQ8BFY6

Proteomic databases

EPDiQ8BFY6
MaxQBiQ8BFY6
PaxDbiQ8BFY6
PeptideAtlasiQ8BFY6
PRIDEiQ8BFY6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030563; ENSMUSP00000030563; ENSMUSG00000028779
ENSMUST00000118199; ENSMUSP00000112638; ENSMUSG00000028779
GeneIDi67898
KEGGimmu:67898
UCSCiuc008uyw.1 mouse

Organism-specific databases

CTDi553115
MGIiMGI:1915148 Pef1

Phylogenomic databases

eggNOGiKOG0037 Eukaryota
ENOG410YKQK LUCA
GeneTreeiENSGT00620000087734
HOGENOMiHOG000231983
HOVERGENiHBG004492
InParanoidiQ8BFY6
OMAiPPQNYGF
OrthoDBiEOG091G0ISY
PhylomeDBiQ8BFY6
TreeFamiTF314682

Miscellaneous databases

PROiPR:Q8BFY6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028779 Expressed in 280 organ(s), highest expression level in colon
CleanExiMM_PEF1
GenevisibleiQ8BFY6 MM

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPEF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BFY6
Secondary accession number(s): Q8VCT5, Q9CYW8, Q9D934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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