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Protein

Phosphoinositide-interacting protein

Gene

Pirt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI).1 Publication

GO - Molecular functioni

  • ion channel binding Source: MGI
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: MGI
  • phosphatidylinositol bisphosphate binding Source: MGI

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide-interacting protein
Gene namesi
Name:Pirt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2443635 Pirt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show impaired responsiveness to noxious heat and capsaicin. Noxious heat- and capsaicin-sensitive currents in DRG neurons are significantly attenuated.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113 – 121KKKQKQRQK → AAAQAQAQA: Abolishes phosphoinositide-binding but does not affect the interaction with TRPV1. 1 Publication9
Mutagenesisi113 – 115KKK → QQQ: Abolishes phosphoinositide-binding and ability to activate TRPV1 without affecting the interaction with TRPV1. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003470611 – 135Phosphoinositide-interacting proteinAdd BLAST135

Proteomic databases

PaxDbiQ8BFY0
PRIDEiQ8BFY0

Expressioni

Tissue specificityi

Strongly expressed in most dorsal root ganglia (DRG) and trigeminal neurons. Expressed by most peptidergic (CGRP+) and non-peptidergic (IB4+) DRG neurons. Weakly expressed in other parts of the peripheral nervous system (PNS) including sympathetic and enteric neurons. Not expressed in the spinal cord.1 Publication

Developmental stagei

First expressed in DRG neurons around embryonic day 11.5, and expression is maintained throughout adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000048070 Expressed in 62 organ(s), highest expression level in dorsal root ganglion
GenevisibleiQ8BFY0 MM

Interactioni

Subunit structurei

Interacts with TRPV1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000128117

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWQ6 Eukaryota
ENOG410YYIW LUCA
GeneTreeiENSGT00390000016891
HOGENOMiHOG000082413
HOVERGENiHBG106144
InParanoidiQ8BFY0
OMAiVWIPIIR
OrthoDBiEOG091G0ZPU
PhylomeDBiQ8BFY0
TreeFamiTF328608

Family and domain databases

InterProiView protein in InterPro
IPR028068 PIRT
PfamiView protein in Pfam
PF15099 PIRT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BFY0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVLPKALEV DERSPESKDL LPSQTASSLC ISSRSESVWT TTPKSNWEIY
60 70 80 90 100
HKPIIIMSVG AAILLFGVAI TCVAYILEEK HKVVQVLRMI GPAFLSLGLM
110 120 130
MLVCGLVWVP IIKKKQKQRQ KSNFFQSLKF FLLNR
Length:135
Mass (Da):15,264
Last modified:March 1, 2003 - v1
Checksum:iE171F42CFEFC86CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU447173 mRNA Translation: ACC60974.1
AK041182 mRNA Translation: BAC30853.1
AK051285 mRNA Translation: BAC34593.1
AK051645 mRNA Translation: BAC34704.1
AK084056 mRNA Translation: BAC39108.1
CH466601 Genomic DNA Translation: EDL10416.1
BC147296 mRNA Translation: AAI47297.1
BC147297 mRNA Translation: AAI47298.1
CCDSiCCDS24849.1
RefSeqiNP_848771.1, NM_178656.3
XP_006532642.1, XM_006532579.3
UniGeneiMm.440256

Genome annotation databases

EnsembliENSMUST00000123434; ENSMUSP00000128117; ENSMUSG00000048070
GeneIDi193003
KEGGimmu:193003
UCSCiuc007jln.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU447173 mRNA Translation: ACC60974.1
AK041182 mRNA Translation: BAC30853.1
AK051285 mRNA Translation: BAC34593.1
AK051645 mRNA Translation: BAC34704.1
AK084056 mRNA Translation: BAC39108.1
CH466601 Genomic DNA Translation: EDL10416.1
BC147296 mRNA Translation: AAI47297.1
BC147297 mRNA Translation: AAI47298.1
CCDSiCCDS24849.1
RefSeqiNP_848771.1, NM_178656.3
XP_006532642.1, XM_006532579.3
UniGeneiMm.440256

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000128117

Proteomic databases

PaxDbiQ8BFY0
PRIDEiQ8BFY0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000123434; ENSMUSP00000128117; ENSMUSG00000048070
GeneIDi193003
KEGGimmu:193003
UCSCiuc007jln.1 mouse

Organism-specific databases

CTDi644139
MGIiMGI:2443635 Pirt

Phylogenomic databases

eggNOGiENOG410IWQ6 Eukaryota
ENOG410YYIW LUCA
GeneTreeiENSGT00390000016891
HOGENOMiHOG000082413
HOVERGENiHBG106144
InParanoidiQ8BFY0
OMAiVWIPIIR
OrthoDBiEOG091G0ZPU
PhylomeDBiQ8BFY0
TreeFamiTF328608

Miscellaneous databases

PROiPR:Q8BFY0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048070 Expressed in 62 organ(s), highest expression level in dorsal root ganglion
GenevisibleiQ8BFY0 MM

Family and domain databases

InterProiView protein in InterPro
IPR028068 PIRT
PfamiView protein in Pfam
PF15099 PIRT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPIRT_MOUSE
AccessioniPrimary (citable) accession number: Q8BFY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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