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Protein

R-spondin-2

Gene

Rspo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors (PubMed:21693646). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (By similarity).By similarity1 Publication

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • heparin binding Source: MGI
  • signaling receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Heparin-binding
Biological processSensory transduction, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-4641263 Regulation of FZD by ubiquitination

Names & Taxonomyi

Protein namesi
Recommended name:
R-spondin-2
Alternative name(s):
Cysteine-rich and single thrombospondin domain-containing protein 2
Short name:
Cristin-2
Short name:
mCristin-2
Roof plate-specific spondin-2
Gene namesi
Name:Rspo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1922667 Rspo2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000023444024 – 243R-spondin-2Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 46PROSITE-ProRule annotation
Disulfide bondi43 ↔ 52PROSITE-ProRule annotation
Disulfide bondi55 ↔ 74PROSITE-ProRule annotation
Disulfide bondi78 ↔ 93PROSITE-ProRule annotation
Disulfide bondi96 ↔ 104PROSITE-ProRule annotation
Disulfide bondi101 ↔ 110PROSITE-ProRule annotation
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi128 ↔ 141PROSITE-ProRule annotation
Disulfide bondi145 ↔ 187PROSITE-ProRule annotation
Disulfide bondi156 ↔ 163PROSITE-ProRule annotation
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi196 ↔ 203PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BFU0
PeptideAtlasiQ8BFU0
PRIDEiQ8BFU0

PTM databases

iPTMnetiQ8BFU0
PhosphoSitePlusiQ8BFU0

Expressioni

Developmental stagei

Detected from day 9.5 in various neural and mesodermal derivatives, mainly along diencephalon. Strongly expressed in limb buds, particularly in the morphogenetically active region such as the apical ectodermal ridge (AER).1 Publication

Gene expression databases

BgeeiENSMUSG00000051920 Expressed in 178 organ(s), highest expression level in primary oocyte
CleanExiMM_RSPO2
GenevisibleiQ8BFU0 MM

Interactioni

Subunit structurei

Interacts with WNT1 (PubMed:16543246). Binds heparin (PubMed:16543246). Interacts with LGR4, LGR5 and LGR6 (PubMed:21693646).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232079, 3 interactors
CORUMiQ8BFU0
STRINGi10090.ENSMUSP00000067325

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8BFU0
SMRiQ8BFU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati90 – 134FUAdd BLAST45
Domaini144 – 204TSP type-1PROSITE-ProRule annotationAdd BLAST61

Domaini

The FU repeat is required for activation and stabilization of beta-catenin.By similarity

Sequence similaritiesi

Belongs to the R-spondin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410KCR4 Eukaryota
ENOG410ZI6H LUCA
GeneTreeiENSGT00390000011447
HOGENOMiHOG000290668
HOVERGENiHBG082751
InParanoidiQ8BFU0
OMAiDNGCIRC
OrthoDBiEOG091G0FYC
PhylomeDBiQ8BFU0
TreeFamiTF331799

Family and domain databases

CDDicd00064 FU, 1 hit
Gene3Di2.20.100.10, 1 hit
InterProiView protein in InterPro
IPR006212 Furin_repeat
IPR009030 Growth_fac_rcpt_cys_sf
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF15913 Furin-like_2, 1 hit
SMARTiView protein in SMART
SM00261 FU, 2 hits
SM00209 TSP1, 1 hit
SUPFAMiSSF57184 SSF57184, 1 hit
SSF82895 SSF82895, 1 hit
PROSITEiView protein in PROSITE
PS50092 TSP1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BFU0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFCLFSFAL IILNCMDYSQ CQGNRWRRNK RASYVSNPIC KGCLSCSKDN
60 70 80 90 100
GCSRCQQKLF FFLRREGMRQ YGECLHSCPS GYYGHRAPDM NRCARCRIEN
110 120 130 140 150
CDSCFSKDFC TKCKVGFYLH RGRCFDECPD GFAPLDETME CVEGCEVGHW
160 170 180 190 200
SEWGTCSRNN RTCGFKWGLE TRTRQIVKKP AKDTIPCPTI AESRRCKMAM
210 220 230 240
RHCPGGKRTP KAKEKRNKKK RRKLIERAQE QHSVFLATDR VNQ
Length:243
Mass (Da):28,276
Last modified:March 1, 2003 - v1
Checksum:iED76A08D61012ED7
GO

Sequence cautioni

The sequence BC052844 differs from that shown. Reason: Erroneous termination at position 153. Translated as Trp.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049891 mRNA Translation: BAC33974.1
AK087485 mRNA Translation: BAC39893.1
BC052844 mRNA No translation available.
CCDSiCCDS27451.1
RefSeqiNP_766403.1, NM_172815.3
XP_006520968.1, XM_006520905.1
UniGeneiMm.193274

Genome annotation databases

EnsembliENSMUST00000063492; ENSMUSP00000067325; ENSMUSG00000051920
ENSMUST00000226810; ENSMUSP00000154600; ENSMUSG00000051920
GeneIDi239405
KEGGimmu:239405
UCSCiuc007vpg.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049891 mRNA Translation: BAC33974.1
AK087485 mRNA Translation: BAC39893.1
BC052844 mRNA No translation available.
CCDSiCCDS27451.1
RefSeqiNP_766403.1, NM_172815.3
XP_006520968.1, XM_006520905.1
UniGeneiMm.193274

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C99X-ray2.80B/D37-144[»]
4UFRX-ray2.20B/D39-144[»]
4UFSX-ray4.80B39-144[»]
ProteinModelPortaliQ8BFU0
SMRiQ8BFU0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232079, 3 interactors
CORUMiQ8BFU0
STRINGi10090.ENSMUSP00000067325

PTM databases

iPTMnetiQ8BFU0
PhosphoSitePlusiQ8BFU0

Proteomic databases

PaxDbiQ8BFU0
PeptideAtlasiQ8BFU0
PRIDEiQ8BFU0

Protocols and materials databases

DNASUi239405
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063492; ENSMUSP00000067325; ENSMUSG00000051920
ENSMUST00000226810; ENSMUSP00000154600; ENSMUSG00000051920
GeneIDi239405
KEGGimmu:239405
UCSCiuc007vpg.1 mouse

Organism-specific databases

CTDi340419
MGIiMGI:1922667 Rspo2

Phylogenomic databases

eggNOGiENOG410KCR4 Eukaryota
ENOG410ZI6H LUCA
GeneTreeiENSGT00390000011447
HOGENOMiHOG000290668
HOVERGENiHBG082751
InParanoidiQ8BFU0
OMAiDNGCIRC
OrthoDBiEOG091G0FYC
PhylomeDBiQ8BFU0
TreeFamiTF331799

Enzyme and pathway databases

ReactomeiR-MMU-4641263 Regulation of FZD by ubiquitination

Miscellaneous databases

PROiPR:Q8BFU0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051920 Expressed in 178 organ(s), highest expression level in primary oocyte
CleanExiMM_RSPO2
GenevisibleiQ8BFU0 MM

Family and domain databases

CDDicd00064 FU, 1 hit
Gene3Di2.20.100.10, 1 hit
InterProiView protein in InterPro
IPR006212 Furin_repeat
IPR009030 Growth_fac_rcpt_cys_sf
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF15913 Furin-like_2, 1 hit
SMARTiView protein in SMART
SM00261 FU, 2 hits
SM00209 TSP1, 1 hit
SUPFAMiSSF57184 SSF57184, 1 hit
SSF82895 SSF82895, 1 hit
PROSITEiView protein in PROSITE
PS50092 TSP1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSPO2_MOUSE
AccessioniPrimary (citable) accession number: Q8BFU0
Secondary accession number(s): Q7TPX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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