UniProtKB - Q8BFR5 (EFTU_MOUSE)
Protein
Elongation factor Tu, mitochondrial
Gene
Tufm
Organism
Mus musculus (Mouse)
Status
Functioni
Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 64 – 71 | GTPBy similarity | 8 | |
Nucleotide bindingi | 126 – 130 | GTPBy similarity | 5 | |
Nucleotide bindingi | 181 – 184 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- translation elongation factor activity Source: UniProtKB
GO - Biological processi
- mitochondrial translational elongation Source: GO_Central
- response to ethanol Source: Ensembl
- translational elongation Source: UniProtKB
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor Tu, mitochondrial |
Gene namesi | Name:Tufm |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923686, Tufm |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrial nucleoid Source: MGI
- mitochondrion Source: MGI
Other locations
- myelin sheath Source: UniProtKB
- synapse Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 43 | MitochondrionBy similarityAdd BLAST | 43 | |
ChainiPRO_0000007463 | 44 – 452 | Elongation factor Tu, mitochondrialAdd BLAST | 409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 79 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 88 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 88 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 234 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 256 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 278 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 286 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 | |
Modified residuei | 361 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 418 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8BFR5 |
jPOSTi | Q8BFR5 |
PaxDbi | Q8BFR5 |
PeptideAtlasi | Q8BFR5 |
PRIDEi | Q8BFR5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q6P919 Q8BFR5 |
PTM databases
iPTMneti | Q8BFR5 |
PhosphoSitePlusi | Q8BFR5 |
SwissPalmi | Q8BFR5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000073838, Expressed in brown adipose tissue and 91 other tissues |
Genevisiblei | Q8BFR5, MM |
Interactioni
Subunit structurei
Interacts with NLRX1.
Interacts with ATG16L1.
By similarityProtein-protein interaction databases
BioGRIDi | 231459, 24 interactors |
IntActi | Q8BFR5, 13 interactors |
MINTi | Q8BFR5 |
STRINGi | 10090.ENSMUSP00000095656 |
Miscellaneous databases
RNActi | Q8BFR5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 251 | tr-type GPROSITE-ProRule annotationAdd BLAST | 197 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 71 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 105 – 109 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 126 – 129 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 181 – 184 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 219 – 221 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0460, Eukaryota |
GeneTreei | ENSGT00940000156748 |
HOGENOMi | CLU_007265_1_0_1 |
InParanoidi | Q8BFR5 |
OMAi | EGDKEWG |
OrthoDBi | 491836at2759 |
PhylomeDBi | Q8BFR5 |
TreeFami | TF300432 |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BFR5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAATLLRAT PRFSGLCASP TPFLQGRLRP LKAPASPFLC RGLAVEAKKT
60 70 80 90 100
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE
110 120 130 140 150
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV
160 170 180 190 200
VAANDGPMPQ TREHLLLAKQ IGVEHVVVYV NKADAVQDSE MVELVELEIR
210 220 230 240 250
ELLTEFGYKG EETPVIVGSA LCALEQRDPE LGVKSVQKLL DAVDTYIPVP
260 270 280 290 300
TRDLDKPFLL PVESVYSIPG RGTVVTGTLE RGILKKGDEC ELLGHNKNIR
310 320 330 340 350
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIQPHQ
360 370 380 390 400
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRVI LPPGKELAMP
410 420 430 440 450
GEDLKLSLIL RQPMILEKGQ RFTLRDGNKT IGTGLVTDVP AMTEEDKNIK
WS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RPC4 | A0A0U1RPC4_MOUSE | Elongation factor Tu, mitochondrial | Tufm | 116 | Annotation score: | ||
A0A0U1RNQ6 | A0A0U1RNQ6_MOUSE | Elongation factor Tu, mitochondrial | Tufm | 118 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013942 | 357 – 452 | YILSK…NIKWS → RAPVLSGFPCLEAGLVAKPF HPYCFSPLGLYPQQGGRWPP QTLCISFHARHVLPDLGHGL SSHLASREGTCHAWRGLEA in isoform 2. 1 PublicationAdd BLAST | 96 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075857 mRNA Translation: BAC36008.1 AK084724 mRNA Translation: BAC39263.1 AK153135 mRNA Translation: BAE31747.1 AK152858 mRNA Translation: BAE31551.1 BC060959 mRNA Translation: AAH60959.1 BC100596 mRNA Translation: AAI00597.1 |
CCDSi | CCDS21830.1 [Q8BFR5-1] CCDS52398.1 [Q8BFR5-2] |
RefSeqi | NP_001157185.1, NM_001163713.1 [Q8BFR5-2] NP_766333.1, NM_172745.3 [Q8BFR5-1] |
Genome annotation databases
Ensembli | ENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838 [Q8BFR5-1] ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838 [Q8BFR5-2] |
GeneIDi | 233870 |
KEGGi | mmu:233870 |
UCSCi | uc009jro.2, mouse [Q8BFR5-1] uc009jrq.2, mouse [Q8BFR5-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075857 mRNA Translation: BAC36008.1 AK084724 mRNA Translation: BAC39263.1 AK153135 mRNA Translation: BAE31747.1 AK152858 mRNA Translation: BAE31551.1 BC060959 mRNA Translation: AAH60959.1 BC100596 mRNA Translation: AAI00597.1 |
CCDSi | CCDS21830.1 [Q8BFR5-1] CCDS52398.1 [Q8BFR5-2] |
RefSeqi | NP_001157185.1, NM_001163713.1 [Q8BFR5-2] NP_766333.1, NM_172745.3 [Q8BFR5-1] |
3D structure databases
SMRi | Q8BFR5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 231459, 24 interactors |
IntActi | Q8BFR5, 13 interactors |
MINTi | Q8BFR5 |
STRINGi | 10090.ENSMUSP00000095656 |
PTM databases
iPTMneti | Q8BFR5 |
PhosphoSitePlusi | Q8BFR5 |
SwissPalmi | Q8BFR5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q6P919 Q8BFR5 |
Proteomic databases
EPDi | Q8BFR5 |
jPOSTi | Q8BFR5 |
PaxDbi | Q8BFR5 |
PeptideAtlasi | Q8BFR5 |
PRIDEi | Q8BFR5 |
Protocols and materials databases
Antibodypediai | 13151, 298 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838 [Q8BFR5-1] ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838 [Q8BFR5-2] |
GeneIDi | 233870 |
KEGGi | mmu:233870 |
UCSCi | uc009jro.2, mouse [Q8BFR5-1] uc009jrq.2, mouse [Q8BFR5-2] |
Organism-specific databases
CTDi | 7284 |
MGIi | MGI:1923686, Tufm |
Phylogenomic databases
eggNOGi | KOG0460, Eukaryota |
GeneTreei | ENSGT00940000156748 |
HOGENOMi | CLU_007265_1_0_1 |
InParanoidi | Q8BFR5 |
OMAi | EGDKEWG |
OrthoDBi | 491836at2759 |
PhylomeDBi | Q8BFR5 |
TreeFami | TF300432 |
Miscellaneous databases
BioGRID-ORCSi | 233870, 9 hits in 18 CRISPR screens |
ChiTaRSi | Tufm, mouse |
PROi | PR:Q8BFR5 |
RNActi | Q8BFR5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000073838, Expressed in brown adipose tissue and 91 other tissues |
Genevisiblei | Q8BFR5, MM |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EFTU_MOUSE | |
Accessioni | Q8BFR5Primary (citable) accession number: Q8BFR5 Secondary accession number(s): Q497E7, Q6P919 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families