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Protein

Elongation factor Tu, mitochondrial

Gene

Tufm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 71GTPBy similarity8
Nucleotide bindingi126 – 130GTPBy similarity5
Nucleotide bindingi181 – 184GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • mitochondrial translational elongation Source: GO_Central
  • response to ethanol Source: Ensembl
  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Gene namesi
Name:Tufm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1923686 Tufm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionBy similarityAdd BLAST43
ChainiPRO_000000746344 – 452Elongation factor Tu, mitochondrialAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysineBy similarity1
Modified residuei88N6-acetyllysine; alternateBy similarity1
Modified residuei88N6-succinyllysine; alternateCombined sources1
Modified residuei234N6-succinyllysineCombined sources1
Modified residuei256N6-acetyllysineCombined sources1
Modified residuei278PhosphothreonineBy similarity1
Modified residuei286N6-succinyllysineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei361N6-acetyllysineCombined sources1
Modified residuei418N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BFR5
PaxDbiQ8BFR5
PeptideAtlasiQ8BFR5
PRIDEiQ8BFR5

2D gel databases

REPRODUCTION-2DPAGEiQ6P919
Q8BFR5

PTM databases

iPTMnetiQ8BFR5
PhosphoSitePlusiQ8BFR5
SwissPalmiQ8BFR5

Expressioni

Gene expression databases

BgeeiENSMUSG00000073838 Expressed in 58 organ(s), highest expression level in brown adipose tissue
CleanExiMM_TUFM
ExpressionAtlasiQ8BFR5 baseline and differential
GenevisibleiQ8BFR5 MM

Interactioni

Subunit structurei

Interacts with NLRX1. Interacts with ATG16L1.By similarity

Protein-protein interaction databases

BioGridi231459, 7 interactors
IntActiQ8BFR5, 13 interactors
MINTiQ8BFR5
STRINGi10090.ENSMUSP00000095656

Structurei

3D structure databases

ProteinModelPortaliQ8BFR5
SMRiQ8BFR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 251tr-type GPROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 71G1PROSITE-ProRule annotation8
Regioni105 – 109G2PROSITE-ProRule annotation5
Regioni126 – 129G3PROSITE-ProRule annotation4
Regioni181 – 184G4PROSITE-ProRule annotation4
Regioni219 – 221G5PROSITE-ProRule annotation3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
GeneTreeiENSGT00550000074682
HOGENOMiHOG000229290
HOVERGENiHBG001535
InParanoidiQ8BFR5
KOiK02358
OMAiYGHIDCP
OrthoDBiEOG091G0BFD
PhylomeDBiQ8BFR5
TreeFamiTF300432

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BFR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAATLLRAT PRFSGLCASP TPFLQGRLRP LKAPASPFLC RGLAVEAKKT
60 70 80 90 100
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE
110 120 130 140 150
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV
160 170 180 190 200
VAANDGPMPQ TREHLLLAKQ IGVEHVVVYV NKADAVQDSE MVELVELEIR
210 220 230 240 250
ELLTEFGYKG EETPVIVGSA LCALEQRDPE LGVKSVQKLL DAVDTYIPVP
260 270 280 290 300
TRDLDKPFLL PVESVYSIPG RGTVVTGTLE RGILKKGDEC ELLGHNKNIR
310 320 330 340 350
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIQPHQ
360 370 380 390 400
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRVI LPPGKELAMP
410 420 430 440 450
GEDLKLSLIL RQPMILEKGQ RFTLRDGNKT IGTGLVTDVP AMTEEDKNIK

WS
Length:452
Mass (Da):49,508
Last modified:March 1, 2003 - v1
Checksum:iF8687A05FFCB337D
GO
Isoform 2 (identifier: Q8BFR5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-452: YILSKEEGGR...TEEDKNIKWS → RAPVLSGFPC...TCHAWRGLEA

Note: No experimental confirmation available.
Show »
Length:435
Mass (Da):47,222
Checksum:i6821088F0D8A5145
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPC4A0A0U1RPC4_MOUSE
Elongation factor Tu, mitochondrial
Tufm
116Annotation score:
A0A0U1RNQ6A0A0U1RNQ6_MOUSE
Elongation factor Tu, mitochondrial
Tufm
118Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013942357 – 452YILSK…NIKWS → RAPVLSGFPCLEAGLVAKPF HPYCFSPLGLYPQQGGRWPP QTLCISFHARHVLPDLGHGL SSHLASREGTCHAWRGLEA in isoform 2. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075857 mRNA Translation: BAC36008.1
AK084724 mRNA Translation: BAC39263.1
AK153135 mRNA Translation: BAE31747.1
AK152858 mRNA Translation: BAE31551.1
BC060959 mRNA Translation: AAH60959.1
BC100596 mRNA Translation: AAI00597.1
CCDSiCCDS21830.1 [Q8BFR5-1]
CCDS52398.1 [Q8BFR5-2]
RefSeqiNP_001157185.1, NM_001163713.1 [Q8BFR5-2]
NP_766333.1, NM_172745.3 [Q8BFR5-1]
UniGeneiMm.197829

Genome annotation databases

EnsembliENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838 [Q8BFR5-1]
ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838 [Q8BFR5-2]
GeneIDi233870
KEGGimmu:233870
UCSCiuc009jro.2 mouse [Q8BFR5-1]
uc009jrq.2 mouse [Q8BFR5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075857 mRNA Translation: BAC36008.1
AK084724 mRNA Translation: BAC39263.1
AK153135 mRNA Translation: BAE31747.1
AK152858 mRNA Translation: BAE31551.1
BC060959 mRNA Translation: AAH60959.1
BC100596 mRNA Translation: AAI00597.1
CCDSiCCDS21830.1 [Q8BFR5-1]
CCDS52398.1 [Q8BFR5-2]
RefSeqiNP_001157185.1, NM_001163713.1 [Q8BFR5-2]
NP_766333.1, NM_172745.3 [Q8BFR5-1]
UniGeneiMm.197829

3D structure databases

ProteinModelPortaliQ8BFR5
SMRiQ8BFR5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231459, 7 interactors
IntActiQ8BFR5, 13 interactors
MINTiQ8BFR5
STRINGi10090.ENSMUSP00000095656

PTM databases

iPTMnetiQ8BFR5
PhosphoSitePlusiQ8BFR5
SwissPalmiQ8BFR5

2D gel databases

REPRODUCTION-2DPAGEiQ6P919
Q8BFR5

Proteomic databases

EPDiQ8BFR5
PaxDbiQ8BFR5
PeptideAtlasiQ8BFR5
PRIDEiQ8BFR5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838 [Q8BFR5-1]
ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838 [Q8BFR5-2]
GeneIDi233870
KEGGimmu:233870
UCSCiuc009jro.2 mouse [Q8BFR5-1]
uc009jrq.2 mouse [Q8BFR5-2]

Organism-specific databases

CTDi7284
MGIiMGI:1923686 Tufm

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
GeneTreeiENSGT00550000074682
HOGENOMiHOG000229290
HOVERGENiHBG001535
InParanoidiQ8BFR5
KOiK02358
OMAiYGHIDCP
OrthoDBiEOG091G0BFD
PhylomeDBiQ8BFR5
TreeFamiTF300432

Miscellaneous databases

PROiPR:Q8BFR5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073838 Expressed in 58 organ(s), highest expression level in brown adipose tissue
CleanExiMM_TUFM
ExpressionAtlasiQ8BFR5 baseline and differential
GenevisibleiQ8BFR5 MM

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU_MOUSE
AccessioniPrimary (citable) accession number: Q8BFR5
Secondary accession number(s): Q497E7, Q6P919
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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