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Protein

Nucleoprotein

Gene

N

Organism
Tamiami mammarenavirus (isolate Rat/United States/W 10777/1964) (TAMV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and therby participates in the suppression of interferon induction.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi381ManganeseUniRule annotation1
Metal bindingi383ManganeseUniRule annotation1
Metal bindingi391ZincUniRule annotation1
Sitei458Important for exonuclease activityUniRule annotation1
Metal bindingi498ZincUniRule annotation1
Metal bindingi501Zinc; via tele nitrogenUniRule annotation1
Metal bindingi522ZincUniRule annotation1
Metal bindingi526ManganeseUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ribonucleoprotein, RNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NucleoproteinUniRule annotation (EC:3.1.13.-UniRule annotation)
Alternative name(s):
Nucleocapsid proteinUniRule annotation
Protein NUniRule annotation
Gene namesi
Name:NUniRule annotation
OrganismiTamiami mammarenavirus (isolate Rat/United States/W 10777/1964) (TAMV)
Taxonomic identifieri45223 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesArenaviridaeMammarenavirus
Virus hostiSigmodon hispidus (Hispid cotton rat) [TaxID: 42415]
Proteomesi
  • UP000172871 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003610151 – 562NucleoproteinAdd BLAST562

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.UniRule annotation

Structurei

3D structure databases

SMRiQ8BD31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 238Binding site for the cap structure m7GTPUniRule annotationAdd BLAST186

Domaini

The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.UniRule annotation

Sequence similaritiesi

Belongs to the arenaviridae nucleocapsid protein family.UniRule annotation

Family and domain databases

Gene3Di3.30.420.410, 1 hit
HAMAPiMF_04085 ARENA_NCAP, 1 hit
InterProiView protein in InterPro
IPR000229 Nucleocapsid_arenaviridae
IPR035084 Nucleocapsid_C_arenaviridae
IPR038115 Nucleocapsid_C_sf
IPR035083 Nucleocapsid_N_arenaviridae
IPR012337 RNaseH-like_sf
PfamiView protein in Pfam
PF17290 Arena_ncap_C, 1 hit
PF00843 Arena_nucleocap, 1 hit
PIRSFiPIRSF004029 N_ArenaV, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BD31-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDQSVPSFR WVQSLKRGLS VWTTPVKADV LNDTRALLSG LDFSKVASVQ
60 70 80 90 100
RMMRRERRDD NDLTNLRDNL KEVDSLMTMR SSQKNMFLKV GSLSKDELME
110 120 130 140 150
LSSDLNKLKE KVQRSERIIG GSGVYQGNFT TTHLTRRSEI LQLVGIQRPG
160 170 180 190 200
LNRRGGVVKI WDIKEPALLI NQFGSTPAVT ISCMAEQGGE TLNDVVQGLT
210 220 230 240 250
DLGLLYTAKF PNLGDLEALS NKHSCLKVIT QEESQINISG FNLSLSAAVK
260 270 280 290 300
AGACLVDGGN MLETIKVEES TFTTIIKTLL EIKSKERMFV DITPGQRNPY
310 320 330 340 350
ENLLYKLCLS GEGWPYIASR SQIKGRAWDN TVIEFDVSPR KPPVPIRNGG
360 370 380 390 400
SPVLTTLKPE VEEQIKRSIE SLSVHDTTWI DIEGPPFDPV EMAIYQPDSL
410 420 430 440 450
KYIHCYRKPN DVKSFKDQSK YCHGILLKDI EFARPGIISA IIKHLPKSMV
460 470 480 490 500
FTAQGSEDIR RLFDMHGRQD LKIVDVKFTA EQSRVFEELT WKRFEHLCDK
510 520 530 540 550
HKGIVIKSKK KGTTPASTNA HCALMDCIMF SGVLLGAIPN DKPRRLLPLD
560
ILFREPDTTV VL
Length:562
Mass (Da):63,166
Last modified:March 1, 2003 - v1
Checksum:i1FCC129C2447A3AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512828 Genomic RNA Translation: AAN32956.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512828 Genomic RNA Translation: AAN32956.1

3D structure databases

SMRiQ8BD31
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.420.410, 1 hit
HAMAPiMF_04085 ARENA_NCAP, 1 hit
InterProiView protein in InterPro
IPR000229 Nucleocapsid_arenaviridae
IPR035084 Nucleocapsid_C_arenaviridae
IPR038115 Nucleocapsid_C_sf
IPR035083 Nucleocapsid_N_arenaviridae
IPR012337 RNaseH-like_sf
PfamiView protein in Pfam
PF17290 Arena_ncap_C, 1 hit
PF00843 Arena_nucleocap, 1 hit
PIRSFiPIRSF004029 N_ArenaV, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_TAMVU
AccessioniPrimary (citable) accession number: Q8BD31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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