UniProtKB - Q8BCR0 (POLN_RUBVN)
Non-structural polyprotein p200
Functioni
Probable principal replicase for the negative-strand DNA, which replicates the 40S (+) genomic RNA into (-) antigenomic RNA. It cannot replicate the (-) into (+) until cleaved into p150 and p90 mature proteins.
By similarityProtease that cleaves the precursor polyprotein into two mature products. Together with RNA-directed RNA polymerase p90, replicates the 40S genomic and antigenomic RNA by recognizing replications specific signals. The heterodimer P150/p90 is probably the principal replicase for positive-strand genomic RNA and the 24S subgenomic RNA, which codes for structural proteins. Responsible for the mRNA-capping of the viral mRNAs. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Forms fibers late in the infection that may be involved in cell-to-cell spread of the virus RNA in the absence of virus particle formation.
By similarityTogether with protease/methyltransferase p150, replicates the 40S genomic and antigenomic RNA by recognizing replications specific signals. The heterodimer P150/p90 is probably the principal replicase for positive-strand genomic RNA and the 24S subgenomic RNA, which codes for structural proteins. A helicase activity is probably also present.
By similarityMiscellaneous
Catalytic activityi
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)PROSITE-ProRule annotationBy similarityEC:2.7.7.48PROSITE-ProRule annotationBy similarity
- a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H+ + phosphateBy similarityEC:3.6.1.15By similarity
- EC:3.6.4.13By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1152 | For cysteine protease activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 1175 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 1178 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 1227 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 1273 | For cysteine protease activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 1273 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1352 – 1359 | NTPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- cysteine-type endopeptidase activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- mRNA methyltransferase activity Source: InterPro
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- RNA processing Source: InterPro
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase |
Biological process | Viral RNA replication |
Ligand | ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc |
Protein family/group databases
MEROPSi | C27.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural polyprotein p200Short name: p200 Cleaved into the following 2 chains: RNA-directed RNA polymerase p90 (EC:2.7.7.48PROSITE-ProRule annotation, EC:3.6.1.15, EC:3.6.4.13) Short name: p90 |
Organismi | Rubella virus (strain RN-UK86) (RUBV) |
Taxonomic identifieri | 376267 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Alsuviricetes › Hepelivirales › Matonaviridae › Rubivirus › Rubivirus rubellae › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host membrane By similarity
- host perinuclear region By similarity
- Host cytoplasm By similarity Note: Localizes to cytoplasmic foci at 24 hpi.By similarity
- Host membrane By similarity
- host perinuclear region By similarity
- Host cytoplasm By similarity Note: At 36 hpi, localizes to the host cytoplasm, probably in vesicles inside host vacuoles of endosomal and lysosomal origin (By similarity). At 72 hpi, localizes to filamentous structures in the host cytoplasm (By similarity).By similarity
- Host membrane By similarity
- Host cytoplasm By similarity Note: Localizes to the cytoplasm and to the cytoplasmic fibers formed by protease/methyltransferase p150.By similarity
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000240170 | 1 – 2116 | Non-structural polyprotein p200Add BLAST | 2116 | |
ChainiPRO_0000240171 | 1 – 1301 | Protease/methyltransferase p150Add BLAST | 1301 | |
ChainiPRO_0000240172 | 1302 – 2116 | RNA-directed RNA polymerase p90Add BLAST | 815 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 1301 – 1302 | Cleavage; autocatalyticPROSITE-ProRule annotation | 2 |
Interactioni
Subunit structurei
Interacts with RNA-directed RNA polymerase p90.
Interacts with host CALM1; this interaction is necessary for the protease activity and viral infectivity.
Interacts with host C1QBP.
Interacts with the capsid protein.
By similarityInteracts with human RB1/retinoblastoma protein.
Interacts with protease/methyltransferase p150.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 247 | Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST | 191 | |
Domaini | 806 – 985 | MacroPROSITE-ProRule annotationAdd BLAST | 180 | |
Domaini | 1000 – 1301 | Peptidase C27PROSITE-ProRule annotationAdd BLAST | 302 | |
Domaini | 1320 – 1468 | (+)RNA virus helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 149 | |
Domaini | 1469 – 1609 | (+)RNA virus helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 141 | |
Domaini | 1870 – 1981 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 112 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 49 | Required for efficient proteolysis and P150-P90 interactionBy similarityAdd BLAST | 14 | |
Regioni | 715 – 781 | DisorderedSequence analysisAdd BLAST | 67 | |
Regioni | 991 – 1030 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 1152 – 1183 | Interaction with host CALM1PROSITE-ProRule annotationAdd BLAST | 32 | |
Regioni | 1193 – 1228 | EF-hand-likePROSITE-ProRule annotationAdd BLAST | 36 | |
Regioni | 1700 – 1900 | Involved in P150-P90 interactionBy similarityAdd BLAST | 201 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 727 – 732 | PxxPxR; class II SH3-bindingBy similarity | 6 | |
Motifi | 747 – 752 | PxxPxR; class II SH3-bindingBy similarity | 6 | |
Motifi | 761 – 766 | PxxPxR; class II SH3-bindingBy similarity | 6 | |
Motifi | 1902 – 1906 | Human RB1 bindingBy similarity | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 747 – 771 | Pro residuesSequence analysisAdd BLAST | 25 |
Domaini
Family and domain databases
CDDi | cd21557, Macro_X_Nsp3-like, 1 hit |
Gene3Di | 3.40.220.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR002588, Alphavirus-like_MT_dom IPR043502, DNA/RNA_pol_sf IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR027417, P-loop_NTPase IPR008738, Peptidase_C27 IPR007094, RNA-dir_pol_PSvirus IPR022245, Rubi_NSP_C IPR044070, RUBV_NS_PRO IPR001788, Tymovirus_RNA-dep_RNA_pol |
Pfami | View protein in Pfam PF01661, Macro, 1 hit PF05407, Peptidase_C27, 1 hit PF00978, RdRP_2, 1 hit PF12601, Rubi_NSP_C, 1 hit PF01443, Viral_helicase1, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51743, ALPHAVIRUS_MT, 1 hit PS51154, MACRO, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS51889, RUBV_NS_PRO, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MEKLLDEVLA PGGPYNLTVG SWVRDHVRSI VEGAWEVRDV VSAAQKRAIV
60 70 80 90 100
AVIPRPVFTQ MQVSDHPALH AISRYTRRHW IEWGPKEALH VLIDPSPGLL
110 120 130 140 150
REVARVERRW VALCLHRTAR KLATALAETA SEAWHADYVC ALRGAPSGPF
160 170 180 190 200
YVHPEDVPHG GRAVADRCLL YYTPMQMCEL MRTIDATLLV AVDLWPVALA
210 220 230 240 250
AHVGDDWDDL GIAWHLDHDG GCPADCRGAG AGPTPGYTRP CTTRIYQVLP
260 270 280 290 300
DTAHPGRLYR CGPRLWTRDC AVAELSWEVA QHCGHQARVR AVRCTLPIRH
310 320 330 340 350
VRSLQPSARV RLPDLVHLAE VGRWRWFSLP RPVFQRMLSY CKTLSPDAYY
360 370 380 390 400
SERVFKFKNA LSHSITLAGN VLQEGWKGTC AEEDALCAYV AFRAWQSNAR
410 420 430 440 450
LAGIMKSAKR CAADSLSVAG WLDTIWDAIK RFFGSVPLAE RMEEWEQDAA
460 470 480 490 500
VAAFDRGPLE DGGRHLDTVQ PPKSPPRPEI AATWIVHAAS ADRHCACAPR
510 520 530 540 550
CDVPRERPSA PACPPDDEAL IPPWLFAERR ALRCREWDFE ALRARADTAA
560 570 580 590 600
APAPLAPRPA RYPTVLYRHP AHHGPWLTLD EPGEADAALV LCDPLGQPLR
610 620 630 640 650
GPERHFAAGA HMCAQARGLQ AFVRVVPPPE RPWADGGARA WAKFFRGCAW
660 670 680 690 700
AQRLLGEPAV MHLPYTDGDV PKLIALALRT LAQQGAALAL SVRDLPGGAA
710 720 730 740 750
FDANAVTAAV RAGPGQFAAT SPPPGDPPPP RRARRSQRHS DARGTPPPAP
760 770 780 790 800
ARDPPPPAPS PPAPPRAGDP DSPTSAEPAD RARHAELEVV YEPSGPPTST
810 820 830 840 850
KADPDSDIVE SYARAAGPVH LRVRDIMDPP PGCKVVVNAA NEGLLAGSGV
860 870 880 890 900
CGAIFANATA ALAADCRRLA PCPTGEAVAT PGHGCGYTHI IHAVAPRRPR
910 920 930 940 950
DPAALEEGEA LLERAYRSIV ALAAARRWAC VACPLLGAGV YGWSAAESLR
960 970 980 990 1000
AALAATRTEP AERVSLHICH PDRATLTHAS VLVGAGLAAR RVSPPPTEPL
1010 1020 1030 1040 1050
ASCPAGDLGR PAQRSASPPA TPLGDATAPE PRGCQGCELC RCTRVTNDRA
1060 1070 1080 1090 1100
YVNLWLERDR GATSWAMRIP EVVVYGPEHL ATHFPLNHYS VLKPAEVRPP
1110 1120 1130 1140 1150
RGMCGSDMWR CRGWQGMPQV RCTPSNAHAA LCRTGVPPRV STRGGELDPN
1160 1170 1180 1190 1200
TCWLRAAANV AQAARACGAY TSAGCPKCAY GRALSEARTH EDFAALSQRW
1210 1220 1230 1240 1250
SASHADASPD GTGDPLDPLM ETVGCACSRV WVGTEHEAPP DHLLVSLHRA
1260 1270 1280 1290 1300
PNGPWGVVLE VRARPEGGNP TGHFVCAVGG GPRRVSDRPH LWLAVPLSRG
1310 1320 1330 1340 1350
GGTCAATDEG LAQAYYDDLE VRRLGDDAMA RAALASVQRP RKGPYNIRVW
1360 1370 1380 1390 1400
NMAAGAGKTT RILAAFTRED LYVCPTNALL HEIQAKLRAR DIDIKNAATY
1410 1420 1430 1440 1450
ERALTKPLAA YRRIYIDEAF TLGGEYCAFV ASQTTAEVIC VGDRDQCGPH
1460 1470 1480 1490 1500
YANNCRTPVP DRWPTERSRH TWRFPDCWAA RLRAGLDYDI EGERTGTFAC
1510 1520 1530 1540 1550
NLWDGRQVDL HLAFSRETVR RLHEAGIRAY TVREAQGMSV GTACIHVGRD
1560 1570 1580 1590 1600
GTDVALALTR DLAIVSLTRA SDALYLHELE DGSLRAAGLS AFLDAGALAE
1610 1620 1630 1640 1650
LKEVPAGIDR VVAVEQAPPP LPPADGIPEA QDVPPFCPRT LEELVFGRAG
1660 1670 1680 1690 1700
HPHYADLNRV TEGEREVRYM RISRHLLNKN HTEMPGTERV LSAVCAVRRY
1710 1720 1730 1740 1750
RAGEDGSTLR TAVARQHPRP FRQIPPPRVT AGVAQEWRMT YLRERIDLTD
1760 1770 1780 1790 1800
VYTQMGVAAR ELTDRYARRY PEIFAGMCTA QSLSVPAFLK ATLKCVDAAL
1810 1820 1830 1840 1850
GPRDTEDCHA AQGKAGLEIR AWAKEWVQVM SPHFRAIQKI IMRALRPQFL
1860 1870 1880 1890 1900
VAAGHTEPEV DAWWQAHYTT NAIEVDFTEF DMNQTLATRD VELEISAALL
1910 1920 1930 1940 1950
GLPCAEDYRA LRAGSYCTLR ELGSTETGCE RTSGEPATLL HNTTVAMCMA
1960 1970 1980 1990 2000
MRMVPKGVRW AGIFQGDDMV IFLPEGARSA ALKWTPAEVG LFGFHIPVKH
2010 2020 2030 2040 2050
VSTPTPSFCG HVGTAAGLFH DVMHQAIKVL CRRFDPDVLE EQQVALLDRL
2060 2070 2080 2090 2100
RGVYAALPDT VAANAAYYDY SAERVLAIVR ELTAYARGRG LDHPATIGAL
2110
EEIQTPYARA NLHDAD
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 427 | D → G in strain: Isolate SUR-SK74 and ULR-GE84. | 1 | |
Natural varianti | 672 | K → Q in strain: Isolate SUR-SK74 and ULR-GE84. | 1 | |
Natural varianti | 697 | G → R in strain: Isolate ULR-GE84. | 1 | |
Natural varianti | 717 | F → L in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 723 | P → T in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 739 | H → P in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 751 | A → V in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 767 | A → V in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 771 – 772 | DS → VP in strain: Isolate SUR-SK74. | 2 | |
Natural varianti | 775 | S → T in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 799 – 800 | ST → PS in strain: Isolate SUR-SK74. | 2 | |
Natural varianti | 825 | D → N in strain: Isolate SUR-SK74 and ULR-GE84. | 1 | |
Natural varianti | 902 | P → H in strain: Isolate ULR-GE84. | 1 | |
Natural varianti | 930 | C → R in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 941 | Y → H in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 958 | T → A in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 1008 | L → P in strain: Isolate SUR-SK74. | 1 | |
Natural varianti | 1042 | C → Y in strain: Isolate SUR-SK74. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF533117 Genomic RNA Translation: AAN07181.1 AF435866 Genomic RNA Translation: AAL31567.1 AF435865 Genomic RNA Translation: AAL31565.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF533117 Genomic RNA Translation: AAN07181.1 AF435866 Genomic RNA Translation: AAL31567.1 AF435865 Genomic RNA Translation: AAL31565.1 |
3D structure databases
SMRi | Q8BCR0 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | C27.001 |
Family and domain databases
CDDi | cd21557, Macro_X_Nsp3-like, 1 hit |
Gene3Di | 3.40.220.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR002588, Alphavirus-like_MT_dom IPR043502, DNA/RNA_pol_sf IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR027417, P-loop_NTPase IPR008738, Peptidase_C27 IPR007094, RNA-dir_pol_PSvirus IPR022245, Rubi_NSP_C IPR044070, RUBV_NS_PRO IPR001788, Tymovirus_RNA-dep_RNA_pol |
Pfami | View protein in Pfam PF01661, Macro, 1 hit PF05407, Peptidase_C27, 1 hit PF00978, RdRP_2, 1 hit PF12601, Rubi_NSP_C, 1 hit PF01443, Viral_helicase1, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51743, ALPHAVIRUS_MT, 1 hit PS51154, MACRO, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS51889, RUBV_NS_PRO, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POLN_RUBVN | |
Accessioni | Q8BCR0Primary (citable) accession number: Q8BCR0 Secondary accession number(s): Q8VA11, Q8VA13 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |