UniProtKB - Q8B9Q8 (PHOSP_HMPVC)
Phosphoprotein
P
Functioni
Plays critical roles in regulating RNA replication and transcription through its interactions with multiple proteins (By similarity).
Tethers the RNA-directed RNA polymerase L to the nucleoprotein-RNA complex (PubMed:31698413).
Recruits the M2-1 protein, a processivity factor that is required for efficient transcription of viral RNA (By similarity).
Acts as a chaperone for neo-synthesized nucleoprotein by forming an N-P complex that preserves N in a monomeric and RNA-free state and prevents the association of nascent N with host cell RNAs (By similarity).
Recruits the host phosphatase PP1 to inclusion bodies to regulate viral transcription (By similarity).
By similarity1 PublicationGO - Molecular functioni
- RNA-directed 5'-3' RNA polymerase activity Source: InterPro
Keywordsi
Biological process | Viral RNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: PhosphoproteinShort name: Protein P |
Gene namesi | Name:P |
Organismi | Human metapneumovirus (strain CAN97-83) (HMPV) |
Taxonomic identifieri | 694067 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Haploviricotina › Monjiviricetes › Mononegavirales › Pneumoviridae › Metapneumovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion By similarity
- Host cytoplasm By similarity Note: Localizes in cytoplasmic inclusion bodies.By similarity
Keywords - Cellular componenti
Host cytoplasm, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000394809 | 1 – 294 | PhosphoproteinAdd BLAST | 294 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 106 | PhosphoserineBy similarity | 1 | |
Modified residuei | 148 | PhosphoserineBy similarity | 1 | |
Modified residuei | 157 | PhosphoserineBy similarity | 1 | |
Modified residuei | 158 | PhosphoserineBy similarity | 1 | |
Modified residuei | 168 | PhosphoserineBy similarity | 1 | |
Modified residuei | 171 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q8B9Q8 |
Interactioni
Subunit structurei
Homotetramer (PubMed:31698413).
Interacts with protein M2-1; the interaction between the two tetramers is required for the anti-termination and elongation transcriptional activities of protein M2-1 (By similarity).
Interacts with host phosphatase PP1; this interaction recruits PP1 to the inclusion bodies (By similarity). Formation of a complex PP1/M2-1/P allows P to target host PP1 phosphatase to the M2-1 substrate (By similarity).
Interacts with the nucleoprotein N; the phosphorylated phosphoprotein P binds to N-RNA complex.
Interacts with the monomeric RNA-free nucleoprotein N (By similarity).
Interacts with RNA-directed RNA polymerase L (via N-terminus); the association of P and L forms the polymerase complex (PubMed:31698413).
By similarity1 PublicationProtein-protein interaction databases
IntActi | Q8B9Q8, 1 interactor |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 28 | Binding to monomeric RNA-free nucleoprotein1 PublicationAdd BLAST | 17 | |
Regioni | 97 – 136 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 123 – 128 | Binding to host phosphatase PP1By similarity | 6 | |
Regioni | 135 – 157 | Binding to protein M2-1By similarityAdd BLAST | 23 | |
Regioni | 169 – 194 | Oligomerization and binding to RNA-directed RNA polymerase L1 PublicationAdd BLAST | 26 | |
Regioni | 251 – 279 | Binding to RNA-directed RNA polymerase LBy similarityAdd BLAST | 29 | |
Regioni | 260 – 294 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 281 – 294 | Binding to the N-RNA complexBy similarityAdd BLAST | 14 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 97 – 120 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 267 – 282 | Acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR003487, Pprotein_pneumovir |
Pfami | View protein in Pfam PF02478, Pneumo_phosprot, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSFPEGKDIL FMGNEAAKLA EAFQKSLRKP SHKRSQSIIG EKVNTVSETL
60 70 80 90 100
ELPTISRPTK PTILSEPKLA WTDKGGAIKT EAKQTIKVMD PIEEEEFTEK
110 120 130 140 150
RVLPSSDGKT PAEKKLKPST NTKKKVSFTP NEPGKYTKLE KDALDLLSDN
160 170 180 190 200
EEEDAESSIL TFEERDTSSL SIEARLESIE EKLSMILGLL RTLNIATAGP
210 220 230 240 250
TAARDGIRDA MIGIREELIA DIIKEAKGKA AEMMEEEMNQ RTKIGNGSVK
260 270 280 290
LTEKAKELNK IVEDESTSGE SEEEEELKDT QENNQEDDIY QLIM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY145251 Genomic RNA Translation: AAN52865.1 AY297749 Genomic RNA Translation: AAQ67693.1 |
RefSeqi | YP_012606.1, NC_004148.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY145251 Genomic RNA Translation: AAN52865.1 AY297749 Genomic RNA Translation: AAQ67693.1 |
RefSeqi | YP_012606.1, NC_004148.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6U5O | electron microscopy | 3.70 | P/Q/R/S | 1-294 | [»] | |
SMRi | Q8B9Q8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q8B9Q8, 1 interactor |
Proteomic databases
PRIDEi | Q8B9Q8 |
Family and domain databases
InterProi | View protein in InterPro IPR003487, Pprotein_pneumovir |
Pfami | View protein in Pfam PF02478, Pneumo_phosprot, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOSP_HMPVC | |
Accessioni | Q8B9Q8Primary (citable) accession number: Q8B9Q8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | March 1, 2003 | |
Last modified: | September 29, 2021 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families