UniProtKB - Q8AXY1 (PA2A_BOTJR)
Protein
Acidic phospholipase A2 BthA-1
Gene
N/A
Organism
Bothrops jararacussu (Jararacussu)
Status
Functioni
Snake venom phospholipase A2 (PLA2) that induces edema (activity that is inhibited by EDTA and dexamethasone), inhibits phospholipid-dependent collagen/ADP-induced platelet aggregation, possess hypotensive as well as anticoagulant activities. In addition, this enzyme shows bactericidal activity against E.coli and S.aureus. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.2 Publications
Miscellaneous
This enzyme has been found to be not myotoxic, not cytotoxic, not hemorrhagic and not lethal.1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotation1 PublicationEC:3.1.1.4PROSITE-ProRule annotation1 Publication
Cofactori
Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.2 Publications
Activity regulationi
Inhibited by EDTA and BPB.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | CalciumCombined sources2 Publications | 1 | |
Metal bindingi | 47 | Calcium; via amide nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 48 | Calcium; via amide nitrogenCombined sources2 Publications | 1 | |
Active sitei | 63 | 1 Publication | 1 | |
Metal bindingi | 64 | CalciumCombined sources2 Publications | 1 | |
Active sitei | 105 | 1 Publication | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- defense response to bacterium Source: UniProtKB-KW
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
- regulation of blood pressure Source: UniProtKB-KW
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Hypotensive agent, Platelet aggregation inhibiting toxin, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic phospholipase A2 BthA-1 (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): BOJU-III BthA-I-PLA2 Hypotensive phospholipase A2 Phosphatidylcholine 2-acylhydrolase |
Organismi | Bothrops jararacussu (Jararacussu) |
Taxonomic identifieri | 8726 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
LD50 show no toxicity when injected into mice: 25 mg/kg by intraperitoneal injection, 7.5 mg/kg by intravenous injection, and 0.5 mg/kg by intracerebroventricular injection.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000413804 | 17 – 138 | Acidic phospholipase A2 BthA-1Add BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 131 | Combined sources3 Publications | ||
Disulfide bondi | 44 ↔ 60 | Combined sources3 Publications | ||
Disulfide bondi | 59 ↔ 111 | Combined sources3 Publications | ||
Disulfide bondi | 65 ↔ 138 | Combined sources3 Publications | ||
Disulfide bondi | 66 ↔ 104 | Combined sources3 Publications | ||
Disulfide bondi | 73 ↔ 97 | Combined sources3 Publications | ||
Disulfide bondi | 91 ↔ 102 | Combined sources3 Publications |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.2 Publications
Interactioni
Subunit structurei
Homodimer; non-covalently linked.3 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U73 | X-ray | 1.90 | A/B | 17-138 | [»] | |
1UMV | X-ray | 1.79 | X | 17-138 | [»] | |
1Z76 | X-ray | 1.85 | A/B | 17-138 | [»] | |
1ZL7 | X-ray | 1.60 | A | 17-138 | [»] | |
1ZLB | X-ray | 0.97 | A | 17-138 | [»] | |
ProteinModelPortali | Q8AXY1 | |||||
SMRi | Q8AXY1 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8AXY1 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
HOVERGENi | HBG008137 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8AXY1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWIMAVL LVGVEGSLWQ FGKMINYVMG ESGVLQYLSY GCYCGLGGQG
60 70 80 90 100
QPTDATDRCC FVHDCCYGKV TGCDPKIDSY TYSKKNGDVV CGGDDPCKKQ
110 120 130
ICECDRVATT CFRDNKDTYD IKYWFYGAKN CQEKSEPC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY145836 mRNA Translation: AAN37410.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY145836 mRNA Translation: AAN37410.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U73 | X-ray | 1.90 | A/B | 17-138 | [»] | |
1UMV | X-ray | 1.79 | X | 17-138 | [»] | |
1Z76 | X-ray | 1.85 | A/B | 17-138 | [»] | |
1ZL7 | X-ray | 1.60 | A | 17-138 | [»] | |
1ZLB | X-ray | 0.97 | A | 17-138 | [»] | |
ProteinModelPortali | Q8AXY1 | |||||
SMRi | Q8AXY1 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Phylogenomic databases
HOVERGENi | HBG008137 |
Miscellaneous databases
EvolutionaryTracei | Q8AXY1 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | PA2A_BOTJR | |
Accessioni | Q8AXY1Primary (citable) accession number: Q8AXY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2011 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 5, 2018 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references