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Entry version 95 (26 Feb 2020)
Sequence version 1 (01 Jun 2003)
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Protein

1,4-beta-mannosyl-N-acetylglucosamine phosphorylase

Gene

BT_1033

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the N-glycan catabolism by catalyzing the reversible phosphorolysis of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine (ManGlcNAc) in a sequential mechanism to yield N-acetyl-D-glucosamine (GlcNAc) and alpha-D-mannose 1-phosphate. It can also use beta-1,4-D-mannosyl-N-acetyl-D-glucosamine-N,N-diacetylchitobiose (ManGlcNAc2) and N,N-diacetylchitobiose (GlcNAc2) as substrates.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat is 37 sec(-1) for phosphorylase activity with GlcNAc (at pH 5.5 and 30 degrees Celsius). Kcat is 22 sec(-1) for phosphorylase activity with GlcNAc2 (at pH 5.5 and 30 degrees Celsius). Kcat is 210 sec(-1) for phosphorylase activity with ManGlcNAc (at pH 5.5 and 30 degrees Celsius).1 Publication
  1. KM=2.1 mM for ManGlcNAc (at pH 5.5 and 30 degrees Celsius)1 Publication
  2. KM=3.8 mM for GlcNAc (at pH 5.5 and 30 degrees Celsius)1 Publication
  3. KM=27 mM for GlcNAc2 (at pH 5.5 and 30 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 5.5.1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    BTHE:G13PU-5953-MONOMER
    MetaCyc:MONOMER-19019

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    2.4.1.320 709

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q8A8Y4

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GH130 Glycoside Hydrolase Family 130

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    1,4-beta-mannosyl-N-acetylglucosamine phosphorylase1 Publication (EC:2.4.1.3201 Publication)
    Alternative name(s):
    4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Ordered Locus Names:BT_1033
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri226186 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001414 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004307581 – 3221,4-beta-mannosyl-N-acetylglucosamine phosphorylaseAdd BLAST322

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q8A8Y4

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q8A8Y4

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homotetramer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    226186.BT_1033

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q8A8Y4

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyl hydrolase 130 family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105CKB Bacteria
    COG2152 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_046648_0_0_10

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q8A8Y4

    KEGG Orthology (KO)

    More...
    KOi
    K18785

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    KAVQMNI

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    2.115.10.20, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR023296 Glyco_hydro_beta-prop_sf
    IPR007184 Mannoside_phosphorylase

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR34106 PTHR34106, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF04041 Glyco_hydro_130, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF016202 PH1107, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF75005 SSF75005, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q8A8Y4-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MNKIQIPWEE RPVGCTDVMW RYSQNPVIGR YHIPSSNSIF NSAVVPFKDG
    60 70 80 90 100
    FAGVFRCDNK AVQMNIFTGF SKDGIHWDIS HEPIQFKAGN TEMIESEYKY
    110 120 130 140 150
    DPRVTWIEDR YWVTWCNGYH GPTIGIAYTF DFVDFFQCEN AFLPFNRNGV
    160 170 180 190 200
    LFPQKIDGKY AMLSRPSDNG HTPFGDIYIS YSPDMKYWGE HRCVMKVTPF
    210 220 230 240 250
    PESAWQCTKI GAGSVPFLTD EGWLLFYHGV ITTCNGFRYA MGSAILDKDH
    260 270 280 290 300
    PEKVLYRTRE YLIGPAAPYE LQGDVPNVVF PCAALQDGER VAVYYGAADT
    310 320
    VVGMAFGYIQ EIIDFTKRTS II
    Length:322
    Mass (Da):36,759
    Last modified:June 1, 2003 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i266119BBF6380D1E
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE015928 Genomic DNA Translation: AAO76140.1

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_809946.1, NC_004663.1
    WP_011107586.1, NZ_UYXG01000013.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAO76140; AAO76140; BT_1033

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    1074186

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    bth:BT_1033

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|226186.12.peg.1050

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE015928 Genomic DNA Translation: AAO76140.1
    RefSeqiNP_809946.1, NC_004663.1
    WP_011107586.1, NZ_UYXG01000013.1

    3D structure databases

    SMRiQ8A8Y4
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi226186.BT_1033

    Protein family/group databases

    CAZyiGH130 Glycoside Hydrolase Family 130

    Proteomic databases

    PaxDbiQ8A8Y4
    PRIDEiQ8A8Y4

    Genome annotation databases

    EnsemblBacteriaiAAO76140; AAO76140; BT_1033
    GeneIDi1074186
    KEGGibth:BT_1033
    PATRICifig|226186.12.peg.1050

    Phylogenomic databases

    eggNOGiENOG4105CKB Bacteria
    COG2152 LUCA
    HOGENOMiCLU_046648_0_0_10
    InParanoidiQ8A8Y4
    KOiK18785
    OMAiKAVQMNI

    Enzyme and pathway databases

    BioCyciBTHE:G13PU-5953-MONOMER
    MetaCyc:MONOMER-19019
    BRENDAi2.4.1.320 709
    SABIO-RKiQ8A8Y4

    Family and domain databases

    Gene3Di2.115.10.20, 1 hit
    InterProiView protein in InterPro
    IPR023296 Glyco_hydro_beta-prop_sf
    IPR007184 Mannoside_phosphorylase
    PANTHERiPTHR34106 PTHR34106, 1 hit
    PfamiView protein in Pfam
    PF04041 Glyco_hydro_130, 1 hit
    PIRSFiPIRSF016202 PH1107, 1 hit
    SUPFAMiSSF75005 SSF75005, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBMAGP_BACTN
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8A8Y4
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
    Last sequence update: June 1, 2003
    Last modified: February 26, 2020
    This is version 95 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
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