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Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA1

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase 3 (pfkA3), ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 1 (pfkA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi105Magnesium; catalyticUniRule annotation1
Active sitei130Proton acceptorUniRule annotation1
Binding sitei157Allosteric activator ADPUniRule annotation1
Binding sitei165Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei214Allosteric activator ADPUniRule annotation1
Binding sitei225SubstrateUniRule annotation1
Binding sitei251Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75ATPUniRule annotation2
Nucleotide bindingi104 – 107ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE:G13PU-6023-MONOMER
UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:pfkA1UniRule annotation
Ordered Locus Names:BT_1102
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119371 – 327ATP-dependent 6-phosphofructokinase 1Add BLAST327

Proteomic databases

PaxDbiQ8A8R5
PRIDEiQ8A8R5

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi226186.BT_1102

Structurei

3D structure databases

ProteinModelPortaliQ8A8R5
SMRiQ8A8R5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 27Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni128 – 130Substrate bindingUniRule annotation3
Regioni172 – 174Substrate bindingUniRule annotation3
Regioni188 – 190Allosteric activator ADP bindingUniRule annotation3
Regioni216 – 218Allosteric activator ADP bindingUniRule annotation3
Regioni257 – 260Substrate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
InParanoidiQ8A8R5
KOiK00850
OMAiALYDLTF
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8A8R5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDNKYIGILT SGGDASGMNA AIRAVTRAAI FNGFKVKGIY RGYEGLIAGE
60 70 80 90 100
VKELTTEDVS SIIQRGGTIL KTARSETFTT PEGRKKAYKV IQKENINALI
110 120 130 140 150
IIGGDGSLTG ARIFAEEYDV TCIGLPGTID NDLYGTDFTI GYDTALNTIV
160 170 180 190 200
ECVDKIRDTA TSHDRIFFVE VMGRDAGFLA QNSAIASGAE AAIIPEDRTD
210 220 230 240 250
VDQLETFIGR GFRKTKNSSI VIVTESPENK NGGAIYYADR VKKEYPGYDV
260 270 280 290 300
RVSILGHLQR GGAPSANDRI LASRLGEAAI QALMEGQRNV MIGIRNNEIV
310 320
YVPFVQAIKK DKPIDKSLIR VLNELSI
Length:327
Mass (Da):35,511
Last modified:June 16, 2003 - v1
Checksum:iAD8900CDDF1B5479
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO76209.1
RefSeqiNP_810015.1, NC_004663.1
WP_004295279.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO76209; AAO76209; BT_1102
GeneIDi1073299
32503420
KEGGibth:BT_1102
PATRICifig|226186.12.peg.1126

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO76209.1
RefSeqiNP_810015.1, NC_004663.1
WP_004295279.1, NC_004663.1

3D structure databases

ProteinModelPortaliQ8A8R5
SMRiQ8A8R5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_1102

Proteomic databases

PaxDbiQ8A8R5
PRIDEiQ8A8R5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO76209; AAO76209; BT_1102
GeneIDi1073299
32503420
KEGGibth:BT_1102
PATRICifig|226186.12.peg.1126

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
InParanoidiQ8A8R5
KOiK00850
OMAiALYDLTF
OrthoDBiPOG091H01AC

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

BioCyciBTHE:G13PU-6023-MONOMER

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA1_BACTN
AccessioniPrimary (citable) accession number: Q8A8R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: November 7, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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