UniProtKB - Q89AS1 (AHPC_BUCBP)
Protein
Alkyl hydroperoxide reductase C
Gene
bbp_171
Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.26By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 50 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- alkylhydroperoxide reductase activity Source: UniProtKB-EC
- peroxidase activity Source: UniProtKB-KW
- peroxiredoxin activity Source: InterPro
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | BAPH224915:G1G09-174-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Alkyl hydroperoxide reductase C (EC:1.11.1.26By similarity)Alternative name(s): Peroxiredoxin Thioredoxin peroxidase |
Gene namesi | Ordered Locus Names:bbp_171 |
Organismi | Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) |
Taxonomic identifieri | 224915 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Erwiniaceae › Buchnera › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135143 | 1 – 199 | Alkyl hydroperoxide reductase CAdd BLAST | 199 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 50 | Interchain (with C-171); in linked formBy similarity | ||
Disulfide bondi | 171 | Interchain (with C-50); in linked formBy similarity |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.
By similarityProtein-protein interaction databases
STRINGi | 224915.bbp_171 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 163 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 162 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_1_6 |
OMAi | WWPKDFT |
OrthoDBi | 892697at2 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q89AS1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLVTYPAPD FTASAISCNG DIINNFNFKE FTNNQTSILF FWPMDFTFVC
60 70 80 90 100
PSEIIAFNQE LSKFKKRNVK LIGVSIDSVY VHHAWRNTLS HNGQIDKINF
110 120 130 140 150
TMVSDLKREI QRSYGIEHPQ LGVALRATFL IDKNRIIRHQ TINDLPFGRN
160 170 180 190
ISETLRMIDA LHFYEKYGEV CPANWKKGDT GIKTTQEGIH KYLEKISKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016826 Genomic DNA Translation: AAO26904.1 |
RefSeqi | WP_011091305.1, NC_004545.1 |
Genome annotation databases
EnsemblBacteriai | AAO26904; AAO26904; bbp_171 |
KEGGi | bab:bbp_171 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016826 Genomic DNA Translation: AAO26904.1 |
RefSeqi | WP_011091305.1, NC_004545.1 |
3D structure databases
SMRi | Q89AS1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224915.bbp_171 |
Genome annotation databases
EnsemblBacteriai | AAO26904; AAO26904; bbp_171 |
KEGGi | bab:bbp_171 |
Phylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_1_6 |
OMAi | WWPKDFT |
OrthoDBi | 892697at2 |
Enzyme and pathway databases
BioCyci | BAPH224915:G1G09-174-MONOMER |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AHPC_BUCBP | |
Accessioni | Q89AS1Primary (citable) accession number: Q89AS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families