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Protein

Glutamate 5-kinase

Gene

proB

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18ATPUniRule annotation1
Binding sitei58SubstrateUniRule annotation1
Binding sitei145SubstrateUniRule annotation1
Binding sitei157Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178ATPUniRule annotation2
Nucleotide bindingi219 – 225ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTET212717:G1G04-1049-MONOMER
UniPathwayi
UPA00098;UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:CTC_01044
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096621 – 267Glutamate 5-kinaseAdd BLAST267

Interactioni

Protein-protein interaction databases

STRINGi212717.CTC01044

Structurei

3D structure databases

ProteinModelPortaliQ896G3
SMRiQ896G3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
KOiK00931
OMAiPLHTRFI
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q896G3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQFNRKEYLK DVKNIVVKVG SSTLTYENGL LNLFHIEHLV RQLVDLHNRG
60 70 80 90 100
YNVILVSSGA IGAGVGKLGL SEKPKSIPEK QAAAAVGQGI LLHTYEKIFA
110 120 130 140 150
EYGKTIGQIL LTKEDMIDEV RSCNATNTFN ALLDKRVIPI INENDAVVVD
160 170 180 190 200
EIKVGDNDTL SAFVSKLVKA DLLILMSDIE GLYSCDPRIN NDAELINFVE
210 220 230 240 250
KITKDIISCA GGAGTDLGTG GMATKIKAAK IATSAGIPML IVNGATNEVL
260
QDVVEGKSVG TWFNAIK
Length:267
Mass (Da):28,745
Last modified:October 17, 2006 - v2
Checksum:iF6B2A67DEEC55699
GO

Sequence cautioni

The sequence AAO35627 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA Translation: AAO35627.1 Different initiation.
RefSeqiWP_035110487.1, NC_004557.1

Genome annotation databases

EnsemblBacteriaiAAO35627; AAO35627; CTC_01044
GeneIDi24255150
KEGGictc:CTC_01044

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA Translation: AAO35627.1 Different initiation.
RefSeqiWP_035110487.1, NC_004557.1

3D structure databases

ProteinModelPortaliQ896G3
SMRiQ896G3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi212717.CTC01044

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO35627; AAO35627; CTC_01044
GeneIDi24255150
KEGGictc:CTC_01044

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
KOiK00931
OMAiPLHTRFI
OrthoDBiPOG091H00OF

Enzyme and pathway databases

UniPathwayi
UPA00098;UER00359

BioCyciCTET212717:G1G04-1049-MONOMER

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_CLOTE
AccessioniPrimary (citable) accession number: Q896G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 17, 2006
Last modified: October 10, 2018
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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