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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16NAD; via amide nitrogenUniRule annotation1
Binding sitei37NADUniRule annotation1
Binding sitei42NADUniRule annotation1
Binding sitei67NADUniRule annotation1
Binding sitei90SubstrateUniRule annotation1
Binding sitei145NADUniRule annotation1
Binding sitei155Allosteric activatorUniRule annotation1
Binding sitei170Allosteric activatorUniRule annotation1
Active sitei177Proton acceptorUniRule annotation1
Binding sitei232SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 82NADUniRule annotation2
Nucleotide bindingi120 – 122NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciCTET212717:G1G04-2069-MONOMER
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:CTC_01998
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683361 – 316L-lactate dehydrogenaseAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ892U0

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC01998

Structurei

3D structure databases

ProteinModelPortaliQ892U0
SMRiQ892U0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 125Substrate bindingUniRule annotation4
Regioni150 – 153Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
KOiK00016
OMAiMDLMQTA
OrthoDBiPOG091H02EQ

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q892U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKGIKISI IGSGFVGSTT AYALMMEGLA SEIVIVDINK EKAKGEAMDL
60 70 80 90 100
SHGVSFVKPV DIIAGDYEDT KDSDIVIITA GAGPKPGETR LDLINKNYEI
110 120 130 140 150
FKGIVPEVVK YSPKSILLVV SNPVDILTYV TYKLSGFPQE RVIGSGTVLD
160 170 180 190 200
TSRFRYLLGE HFKIDVRNVH TYILGEHGDS EIAAWSLTNI AGISVEDYCK
210 220 230 240 250
DICKGCEGNF KNRIPEEVKN AAYEVLERKG YTSYAIALAV RRIVEAIIRD
260 270 280 290 300
EDSILTVSTL LRGEYGINDI YMGIPSVIGE TGIKRVLEVK LSKDEEKQLK
310
ESAEVLKENL NKIPIK
Length:316
Mass (Da):34,824
Last modified:June 21, 2004 - v2
Checksum:iD95C9DB0EDFF13BC
GO

Sequence cautioni

The sequence AAO36504 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA Translation: AAO36504.1 Different initiation.
RefSeqiWP_035109151.1, NC_004557.1

Genome annotation databases

EnsemblBacteriaiAAO36504; AAO36504; CTC_01998
GeneIDi24254133
KEGGictc:CTC_01998

Similar proteinsi

Entry informationi

Entry nameiLDH_CLOTE
AccessioniPrimary (citable) accession number: Q892U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: May 23, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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