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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei76Charge relay systemUniRule annotation1
Active sitei151Charge relay systemUniRule annotation1
Active sitei175Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1005-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:PP_0931
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001051901 – 483Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST483

Proteomic databases

PRIDEiQ88PB9

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_0931

Structurei

3D structure databases

ProteinModelPortaliQ88PB9
SMRiQ88PB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiMYLSDIF

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q88PB9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHQLTLAEIA RGLADKSFSS EELTGALLAR IKQLDPQINS FISVTEDLAL
60 70 80 90 100
GQARAADARR AAGETSALLG APIAHKDLFC TNGVRTSCGS KMLDNFKAPY
110 120 130 140 150
DATVVAKLAE AGMVTLGKTN MDEFAMGSAN ESSHYGAVKN PWNLEHVPGG
160 170 180 190 200
SSGGSAAAVA ARLLPATTGT DTGGSIRQPA ALTNLTGLKP TYGRVSRWGM
210 220 230 240 250
IAYASSLDQG GPLARTAEDC ALLLQGMAGF DAKDSTSIEE PVPDYSASLN
260 270 280 290 300
ASLQGLRIGL PKEYFGAGLD PRIADLVQAS VKELEKLGAV VKEISLPNMQ
310 320 330 340 350
HAIPAYYVIA PAEASSNLSR FDGVRFGYRC EEPKDLTDLY KRSRGEGFGV
360 370 380 390 400
EVQRRIMVGT YALSAGYYDA YYVKAQQIRR LIKNDFMAAF NDVDLILGPT
410 420 430 440 450
TPNPAWKLGA KSSDPVAAYL EDVYTITANL AGLPGLSMPA GFVDGLPVGV
460 470 480
QLLAPYFQEG RLLNVAHRYQ QVTDWHTRAP NGF
Length:483
Mass (Da):51,518
Last modified:June 1, 2003 - v1
Checksum:iA629E842896C0529
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66556.1
RefSeqiNP_743092.1, NC_002947.4
WP_010952131.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66556; AAN66556; PP_0931
GeneIDi1044835
KEGGippu:PP_0931
PATRICifig|160488.4.peg.991

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66556.1
RefSeqiNP_743092.1, NC_002947.4
WP_010952131.1, NC_002947.4

3D structure databases

ProteinModelPortaliQ88PB9
SMRiQ88PB9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_0931

Proteomic databases

PRIDEiQ88PB9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN66556; AAN66556; PP_0931
GeneIDi1044835
KEGGippu:PP_0931
PATRICifig|160488.4.peg.991

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiMYLSDIF

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1005-MONOMER

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_PSEPK
AccessioniPrimary (citable) accession number: Q88PB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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