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Protein

DNA polymerase IV

Gene

dinB

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Sitei15Substrate discriminationUniRule annotation1
Metal bindingi105MagnesiumUniRule annotation1
Active sitei106UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1288-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Synonyms:dinP
Ordered Locus Names:PP_1203
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739331 – 354DNA polymerase IVAdd BLAST354

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_1203

Structurei

3D structure databases

ProteinModelPortaliQ88NK4
SMRiQ88NK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 187UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiDMQSFYA

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q88NK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRKIIHVD CDCFYAAIEM RDDPRLAGRP MAVGGSPDHR GVIATCNYEA
60 70 80 90 100
RAYGVRSAMS SRHALKLCPD LLIVKPRFEA YREASREIHT IFRDYTELIE
110 120 130 140 150
PLSLDEAYLD VSDSQWYSGS ATRIAEDIRR RVARTLHITV SAGVAPNKFL
160 170 180 190 200
AKIASDWRKP NGLFVITPNE VETFVAALPV ARLHGVGKVT ADKLTRLGIE
210 220 230 240 250
TCLHLREWSR LALVREFGSF GERLWGLARG IDERAVHNDS RRQSVSVENT
260 270 280 290 300
YDTDLPDLAS CLARLPELLD SLNERIARMD SSYRPDKPFV KVKFHDFSQT
310 320 330 340 350
TMEQAGAGRD LESYRQLLGQ AFARGGKPVR LLGVGVRLRD LRGAHEQLEL

FPPK
Length:354
Mass (Da):39,860
Last modified:June 1, 2003 - v1
Checksum:i2C69D16280990018
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66827.1
RefSeqiNP_743363.1, NC_002947.4
WP_010952356.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66827; AAN66827; PP_1203
GeneIDi1045968
KEGGippu:PP_1203
PATRICifig|160488.4.peg.1278

Similar proteinsi

Entry informationi

Entry nameiDPO4_PSEPK
AccessioniPrimary (citable) accession number: Q88NK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health