UniProtKB - Q88FH2 (NUOG_PSEPK)
Protein
NADH-quinone oxidoreductase subunit G
Gene
nuoG
Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 67 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 99 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 106 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 112 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 154 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 157 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 201 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 228 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 231 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 235 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 263 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Translocase |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | PPUT160488:G1G01-4391-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit G (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit G NDH-1 subunit G |
Gene namesi | Name:nuoG Ordered Locus Names:PP_4124 |
Organismi | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) |
Taxonomic identifieri | 160488 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
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PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118551 | 1 – 904 | NADH-quinone oxidoreductase subunit GAdd BLAST | 904 |
Keywords - PTMi
QuinoneInteractioni
Subunit structurei
Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Protein-protein interaction databases
STRINGi | 160488.PP_4124 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 83 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 83 – 122 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 221 – 277 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Phylogenomic databases
eggNOGi | COG1034, Bacteria |
HOGENOMi | CLU_000422_11_4_6 |
OMAi | PSICHGC |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q88FH2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATIHVDGKA LEVNGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYTDENDTR GRIVMSCMTP ASDGTWISID DEESKAFRAS VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG HCHLQDMTVM TGHNERRYRF TKRTHQNQDL GPFIAHEMNR
160 170 180 190 200
CIACYRCVRY YKDYAGGTDL GVYGAHDNVY FGRVEDGVLE SEFSGNLTEV
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG ERYGELRRIE
260 270 280 290 300
NRFNGSVNQY FLCDRGRFGY GYVNRKDRPR QPQLADGTKL GLDAALDKAA
310 320 330 340 350
DLLRGRTIVG IGSPRASLES NYGLRELVGA EYFYSGMEAG ELARVRLALN
360 370 380 390 400
VLNNSPLPVP TLRDIEDHDA VFVLGEDLTQ TAARVALAVR QATKGKAEAM
410 420 430 440 450
AEAMKVQPWL DAAVKNIGQH ALYPLFIASL AETKLDDVAE ECVHAAPADL
460 470 480 490 500
ARIGFAVAHA IDPSAPAVAG LDDEAQALAQ RIADALVAAK RPLVVAGTSL
510 520 530 540 550
ADPALIEAAA NIAKALKLRE KNGSLSLVVP EANSLGLAML GGESVDAALD
560 570 580 590 600
AVISGKADAI VVLENDLYAR VPAAKVDAAL AAAKVVIVAD HSKTATVDRA
610 620 630 640 650
HLVLPAASFA EGDGTLVSQE GRAQRFFQVF DPQYLDSSIQ IHEGWRWMHA
660 670 680 690 700
LRATLLNKPV DWTQLDHVTS ACAEAAPQLA GIVNAAPSAA FRIKGMKLAR
710 720 730 740 750
EPLRYSGRTA MRANISVHEP RTPQDKDTAF AFSMEGYSGS AEPRQQVPFA
760 770 780 790 800
WSPGWNSPQA WNKFQDEVGG HLRAGDPGVR LIESQGDRLN WFNAIPGAFN
810 820 830 840 850
PARGIWTAVP FFHLFGSEES SSRAAPVQER IPAAYVALAK SEADRLGVND
860 870 880 890 900
GALLSLNVAG VALRLPLRIN EELGAGLVAL PKGLAGIPPA IFGASVEGLQ
EAAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE015451 Genomic DNA Translation: AAN69707.1 |
RefSeqi | NP_746243.1, NC_002947.4 WP_010954902.1, NC_002947.4 |
Genome annotation databases
EnsemblBacteriai | AAN69707; AAN69707; PP_4124 |
KEGGi | ppu:PP_4124 |
PATRICi | fig|160488.4.peg.4383 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE015451 Genomic DNA Translation: AAN69707.1 |
RefSeqi | NP_746243.1, NC_002947.4 WP_010954902.1, NC_002947.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 160488.PP_4124 |
Genome annotation databases
EnsemblBacteriai | AAN69707; AAN69707; PP_4124 |
KEGGi | ppu:PP_4124 |
PATRICi | fig|160488.4.peg.4383 |
Phylogenomic databases
eggNOGi | COG1034, Bacteria |
HOGENOMi | CLU_000422_11_4_6 |
OMAi | PSICHGC |
Enzyme and pathway databases
BioCyci | PPUT160488:G1G01-4391-MONOMER |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOG_PSEPK | |
Accessioni | Q88FH2Primary (citable) accession number: Q88FH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families