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Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi67Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi99Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi103Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi106Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi112Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi154Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi201Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi228Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi231Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi235Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi263Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-4391-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
Gene namesi
Name:nuoG
Ordered Locus Names:PP_4124
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185511 – 904NADH-quinone oxidoreductase subunit GAdd BLAST904

Keywords - PTMi

Quinone

Proteomic databases

PRIDEiQ88FH2

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

STRINGi160488.PP_4124

Structurei

3D structure databases

ProteinModelPortaliQ88FH2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 832Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST83
Domaini83 – 1224Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini221 – 2774Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4107R0R Bacteria
COG1034 LUCA
HOGENOMiHOG000276177
KOiK00336
OMAiGYGYVNR

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q88FH2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATIHVDGKA LEVNGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYTDENDTR GRIVMSCMTP ASDGTWISID DEESKAFRAS VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG HCHLQDMTVM TGHNERRYRF TKRTHQNQDL GPFIAHEMNR
160 170 180 190 200
CIACYRCVRY YKDYAGGTDL GVYGAHDNVY FGRVEDGVLE SEFSGNLTEV
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG ERYGELRRIE
260 270 280 290 300
NRFNGSVNQY FLCDRGRFGY GYVNRKDRPR QPQLADGTKL GLDAALDKAA
310 320 330 340 350
DLLRGRTIVG IGSPRASLES NYGLRELVGA EYFYSGMEAG ELARVRLALN
360 370 380 390 400
VLNNSPLPVP TLRDIEDHDA VFVLGEDLTQ TAARVALAVR QATKGKAEAM
410 420 430 440 450
AEAMKVQPWL DAAVKNIGQH ALYPLFIASL AETKLDDVAE ECVHAAPADL
460 470 480 490 500
ARIGFAVAHA IDPSAPAVAG LDDEAQALAQ RIADALVAAK RPLVVAGTSL
510 520 530 540 550
ADPALIEAAA NIAKALKLRE KNGSLSLVVP EANSLGLAML GGESVDAALD
560 570 580 590 600
AVISGKADAI VVLENDLYAR VPAAKVDAAL AAAKVVIVAD HSKTATVDRA
610 620 630 640 650
HLVLPAASFA EGDGTLVSQE GRAQRFFQVF DPQYLDSSIQ IHEGWRWMHA
660 670 680 690 700
LRATLLNKPV DWTQLDHVTS ACAEAAPQLA GIVNAAPSAA FRIKGMKLAR
710 720 730 740 750
EPLRYSGRTA MRANISVHEP RTPQDKDTAF AFSMEGYSGS AEPRQQVPFA
760 770 780 790 800
WSPGWNSPQA WNKFQDEVGG HLRAGDPGVR LIESQGDRLN WFNAIPGAFN
810 820 830 840 850
PARGIWTAVP FFHLFGSEES SSRAAPVQER IPAAYVALAK SEADRLGVND
860 870 880 890 900
GALLSLNVAG VALRLPLRIN EELGAGLVAL PKGLAGIPPA IFGASVEGLQ

EAAQ
Length:904
Mass (Da):97,582
Last modified:June 1, 2003 - v1
Checksum:i6A33D03B3292B3F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN69707.1
RefSeqiNP_746243.1, NC_002947.4
WP_010954902.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN69707; AAN69707; PP_4124
GeneIDi1042269
KEGGippu:PP_4124
PATRICifig|160488.4.peg.4383

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN69707.1
RefSeqiNP_746243.1, NC_002947.4
WP_010954902.1, NC_002947.4

3D structure databases

ProteinModelPortaliQ88FH2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_4124

Proteomic databases

PRIDEiQ88FH2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN69707; AAN69707; PP_4124
GeneIDi1042269
KEGGippu:PP_4124
PATRICifig|160488.4.peg.4383

Phylogenomic databases

eggNOGiENOG4107R0R Bacteria
COG1034 LUCA
HOGENOMiHOG000276177
KOiK00336
OMAiGYGYVNR

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-4391-MONOMER

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUOG_PSEPK
AccessioniPrimary (citable) accession number: Q88FH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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