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Protein

Acetyl-coenzyme A synthetase 1

Gene

acsA1

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei311Coenzyme AUniRule annotation1
Binding sitei335Coenzyme AUniRule annotation1
Binding sitei500ATPUniRule annotation1
Binding sitei515ATPUniRule annotation1
Binding sitei523Coenzyme A; via carbonyl oxygenUniRule annotation1
Binding sitei526ATPUniRule annotation1
Metal bindingi537Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi539Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi542Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei584Coenzyme AUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi387 – 389ATPUniRule annotation3
Nucleotide bindingi411 – 416ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase 1UniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetase 1UniRule annotation
Short name:
Acs 1UniRule annotation
Alternative name(s):
Acetate--CoA ligase 1UniRule annotation
Acyl-activating enzyme 1UniRule annotation
Gene namesi
Name:acsA1UniRule annotation
Ordered Locus Names:PP_4487
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083761 – 653Acetyl-coenzyme A synthetase 1Add BLAST653

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei609N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ88EH6

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_4487

Structurei

3D structure databases

ProteinModelPortaliQ88EH6
SMRiQ88EH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 194Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQF Bacteria
COG0365 LUCA
HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q88EH6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAAPLYPVR PEVAATTLTD EATYKAMYQQ SVINPDGFWR EQAQRIDWIK
60 70 80 90 100
PFTKVKQTSF DDHHVDIKWF ADGTLNVSSN CLDRHLEERG DQLAIIWEGD
110 120 130 140 150
DPSEHRNITY RELHEQVCKF ANALRGQDVH RGDVVTIYMP MIPEAVVAML
160 170 180 190 200
ACARIGAIHS VVFGGFSPEA LAGRIIDCKS KVVITADEGV RGGRRTPLKA
210 220 230 240 250
NVDLALTNPE TSSVQKIIVC KRTGGDIAWH QHRDIWYEDL MKVASSHCAP
260 270 280 290 300
KEMGAEEALF ILYTSGSTGK PKGVLHTTGG YLVYAALTHE RVFDYRPGEV
310 320 330 340 350
YWCTADVGWV TGHSYIVYGP LANGATTLLF EGVPNYPDIT RVSKIVDKHK
360 370 380 390 400
VNILYTAPTA IRAMMAEGQA AVEGADGSSL RLLGSVGEPI NPEAWNWYYK
410 420 430 440 450
TVGKERCPIV DTWWQTETGG ILISPLPGAT GLKPGSATRP FFGVVPALVD
460 470 480 490 500
NLGNLIDGAA EGNLVILDSW PGQSRSLYGD HDRFVDTYFK TFRGMYFTGD
510 520 530 540 550
GARRDEDGYY WITGRVDDVL NVSGHRMGTA EIESAMVAHS KVAEAAVVGV
560 570 580 590 600
PHDIKGQGIY VYVTLNAGIE ASEQLRLELK NWVRKEIGPI ASPDVIQWAP
610 620 630 640 650
GLPKTRSGKI MRRILRKIAT GEYDALGDIS TLADPGVVQH LIDTHKAMNL

ASA
Length:653
Mass (Da):71,797
Last modified:June 1, 2003 - v1
Checksum:iCE626C86C922E8F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN70062.1
RefSeqiNP_746598.2, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN70062; AAN70062; PP_4487
GeneIDi1042436
KEGGippu:PP_4487
PATRICifig|160488.4.peg.4775

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN70062.1
RefSeqiNP_746598.2, NC_002947.4

3D structure databases

ProteinModelPortaliQ88EH6
SMRiQ88EH6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_4487

Proteomic databases

PRIDEiQ88EH6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN70062; AAN70062; PP_4487
GeneIDi1042436
KEGGippu:PP_4487
PATRICifig|160488.4.peg.4775

Phylogenomic databases

eggNOGiENOG4108IQF Bacteria
COG0365 LUCA
HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACSA1_PSEPK
AccessioniPrimary (citable) accession number: Q88EH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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