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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83SubstrateUniRule annotation1
Binding sitei97Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

  • dUTP diphosphatase activity Source: JCVI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • 2'-deoxyribonucleotide metabolic process Source: JCVI
  • dUMP biosynthetic process Source: UniProtKB-UniPathway
  • dUTP metabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-84-MONOMER
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:PSPTO_0084
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828971 – 151Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST151

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_0084

Structurei

3D structure databases

ProteinModelPortaliQ88BD3
SMRiQ88BD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Substrate bindingUniRule annotation3
Regioni87 – 89Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z1K Bacteria
COG0756 LUCA
HOGENOMiHOG000028968
KOiK01520
OMAiGVILINH
OrthoDBiPOG091H01I6
PhylomeDBiQ88BD3

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00116 dUTPase_bact, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

Q88BD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALQAKILD PRIGNEFPLP AYATVGSAGL DLRAMLKEDT LLEPGQTLLI
60 70 80 90 100
PTGLSIYIGD PGLAALILPR SGLGHKHGIV LGNLVGLIDS DYQGELMVSC
110 120 130 140 150
WNRGQTAFTI AVGERIAQLV LVPVVQARFE LVEEFDESQR GTGGFGHSGS

H
Length:151
Mass (Da):16,084
Last modified:June 20, 2003 - v1
Checksum:i6A4E65D73B35826C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO53638.1
RefSeqiNP_789943.1, NC_004578.1
WP_011102984.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO53638; AAO53638; PSPTO_0084
GeneIDi1181692
KEGGipst:PSPTO_0084
PATRICifig|223283.9.peg.88

Similar proteinsi

Entry informationi

Entry nameiDUT_PSESM
AccessioniPrimary (citable) accession number: Q88BD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: February 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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