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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi266Manganese 2UniRule annotation1
Metal bindingi271Manganese 1UniRule annotation1
Metal bindingi271Manganese 2UniRule annotation1
Active sitei278UniRule annotation1
Metal bindingi289Manganese 2UniRule annotation1
Metal bindingi348Manganese 1UniRule annotation1
Metal bindingi350Manganese 1UniRule annotation1
Metal bindingi350Manganese 2UniRule annotation1
Active sitei352UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: JCVI

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-1296-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:PSPTO_1271
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001657851 – 496Probable cytosol aminopeptidaseAdd BLAST496

Proteomic databases

PRIDEiQ887M0

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_1271

Structurei

3D structure databases

ProteinModelPortaliQ887M0
SMRiQ887M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZ6 Bacteria
COG0260 LUCA
HOGENOMiHOG000243132
KOiK01255
OMAiMKNTGPR
OrthoDBiPOG091H01BT
PhylomeDBiQ887M0

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequencei

Sequence statusi: Complete.

Q887M0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELVVKSVSP ETLKTATLVV TINESRVLIG AAQLVDIKSG GAISAVLERG
60 70 80 90 100
DLAGKSGQTL LLTNLQNIKA ERVLLVGIGK DGELSDRQLK KIAGSVLSSL
110 120 130 140 150
KGLGGSDAVI ALDDLSVKNR DTYGKARLFV EALADGEYVF DRFKTQKAEV
160 170 180 190 200
RPLKKITLLT DKVSVADVER AANHAQAIAT GMALTRDLGN LPPNICHPTY
210 220 230 240 250
LGEEAKALGK AHKNLKVEIH DEKKLAELGM GSFLAVAQGS AQPPRLIVMN
260 270 280 290 300
YQGGKKGDKP FVLVGKGITF DTGGISIKPA AGMDEMKFDM CGAASVFGTL
310 320 330 340 350
RAVLELKLPI NVVCILACAE NMPSGTATRP GDIVTTMSGQ TVEILNTDAE
360 370 380 390 400
GRLVLCDALT YAERFKPQAV IDIATLTGAC VVALGGHTSG LLGNNDALIN
410 420 430 440 450
QLLDAGKLAD DRAWQLPLFD EYQEQLDSPF ADIANIGGPK GGTITAACFL
460 470 480 490
SRFTKAYEWA HLDIAGTAWL SGGKDKGATG RPVPLLTQYL LDRAGV
Length:496
Mass (Da):52,389
Last modified:June 1, 2003 - v1
Checksum:iCC597EA0ED671800
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO54796.1
RefSeqiNP_791101.1, NC_004578.1
WP_011103494.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO54796; AAO54796; PSPTO_1271
GeneIDi1182907
KEGGipst:PSPTO_1271
PATRICifig|223283.9.peg.1293

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO54796.1
RefSeqiNP_791101.1, NC_004578.1
WP_011103494.1, NC_004578.1

3D structure databases

ProteinModelPortaliQ887M0
SMRiQ887M0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223283.PSPTO_1271

Proteomic databases

PRIDEiQ887M0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO54796; AAO54796; PSPTO_1271
GeneIDi1182907
KEGGipst:PSPTO_1271
PATRICifig|223283.9.peg.1293

Phylogenomic databases

eggNOGiENOG4105BZ6 Bacteria
COG0260 LUCA
HOGENOMiHOG000243132
KOiK01255
OMAiMKNTGPR
OrthoDBiPOG091H01BT
PhylomeDBiQ887M0

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-1296-MONOMER

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_PSESM
AccessioniPrimary (citable) accession number: Q887M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: October 10, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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