UniProtKB - Q87ZQ4 (NUOG_PSESM)
Protein
NADH-quinone oxidoreductase subunit G
Gene
nuoG
Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 67 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 99 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 106 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 112 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 154 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 157 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 201 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 228 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 231 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 235 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 263 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: JCVI
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Translocase |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | PSYR223283:G1G0D-3425-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit G (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit G NDH-1 subunit G |
Gene namesi | Name:nuoG Ordered Locus Names:PSPTO_3370 |
Organismi | Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000) |
Taxonomic identifieri | 223283 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118552 | 1 – 905 | NADH-quinone oxidoreductase subunit GAdd BLAST | 905 |
Keywords - PTMi
QuinoneInteractioni
Subunit structurei
Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Protein-protein interaction databases
STRINGi | 223283.PSPTO_3370 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 83 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 83 – 122 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 221 – 277 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Phylogenomic databases
eggNOGi | COG1034, Bacteria |
HOGENOMi | CLU_000422_11_4_6 |
OMAi | PSICHGC |
OrthoDBi | 323168at2 |
PhylomeDBi | Q87ZQ4 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR009010, Asp_de-COase-like_dom_sf IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q87ZQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATIHVDGKA LEVDGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYTDENDTR GRIVMSCMTP ATDNTWISIE DDESKAFRAS VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG HCHLQDMTVM TGHNERRYRF TKRTHQNQEL GPFIAHEMNR
160 170 180 190 200
CIACYRCVRF YKDYAGGTDL GVYGAHDNVY FGRVEDGTLE SEFSGNLTEV
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFSPSICHG CSSGCNISPG ERYGELRRIE
260 270 280 290 300
NRYNGSVNQY FLCDRGRFGY GYVNRTDRPR QPLLVDGQVK LSLDEALDKA
310 320 330 340 350
ADLLRGRNIV GIGSPRASLE SNYALRELVG AEHFYSGIEA GELERVRLIL
360 370 380 390 400
KVLNDSPLPI PTLRDIEDHD AIFVLGEDLT QTAARMALGL RQAVKGKAED
410 420 430 440 450
MAAAMKVQPW LDAAVKNIGQ HELNPLFIAS LAETRLDDIA EECVHAAPDD
460 470 480 490 500
LARIGFAVAH ALDASAPAVE GLDSEALELA QRIADALLAA KRPLIISGSS
510 520 530 540 550
LGSTALIEAA ANIAKALKLR DKQGSISLIV PEANSMGLAM FGGDSVDAAL
560 570 580 590 600
QAVISGKADA IVVLENDLYR RVDAKIVDAA LSAAKVVIVA DHQKTPTSDR
610 620 630 640 650
AHLVLPAASF AEGDGTLVSQ EGRAQRFFQV FDPTYLDASI LVHEGWRWLH
660 670 680 690 700
ALRATLLNKP VDWTQLDHVT EACAGSTAQL AGIVNAAPSA SFRIKGLKLA
710 720 730 740 750
REPLRYSGRT AMRANISVHE PRTSQDIDTA FSFSMEGYSG SAEPRQQVPF
760 770 780 790 800
AWSPGWNSPQ AWNKFQDEVG GHLRAGDPGT RLIESQGDGL SWFSSVPRAF
810 820 830 840 850
SPERGTWQVV PFFHLFGSDE TSSLAAPVQE RIPQAYVAVA KSEADRLGVN
860 870 880 890 900
DGALLSLSIG GETLTLPLRI SEELGAGLVA LPAGLPGIPP ALFGKSVDGL
QEAAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016853 Genomic DNA Translation: AAO56848.1 |
RefSeqi | NP_793153.1, NC_004578.1 WP_007246028.1, NC_004578.1 |
Genome annotation databases
EnsemblBacteriai | AAO56848; AAO56848; PSPTO_3370 |
GeneIDi | 58379226 |
KEGGi | pst:PSPTO_3370 |
PATRICi | fig|223283.9.peg.3450 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016853 Genomic DNA Translation: AAO56848.1 |
RefSeqi | NP_793153.1, NC_004578.1 WP_007246028.1, NC_004578.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 223283.PSPTO_3370 |
Genome annotation databases
EnsemblBacteriai | AAO56848; AAO56848; PSPTO_3370 |
GeneIDi | 58379226 |
KEGGi | pst:PSPTO_3370 |
PATRICi | fig|223283.9.peg.3450 |
Phylogenomic databases
eggNOGi | COG1034, Bacteria |
HOGENOMi | CLU_000422_11_4_6 |
OMAi | PSICHGC |
OrthoDBi | 323168at2 |
PhylomeDBi | Q87ZQ4 |
Enzyme and pathway databases
BioCyci | PSYR223283:G1G0D-3425-MONOMER |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR009010, Asp_de-COase-like_dom_sf IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOG_PSESM | |
Accessioni | Q87ZQ4Primary (citable) accession number: Q87ZQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families