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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-4933-MONOMER
UniPathwayi
UPA00074;UER00133

UPA00074;UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:PSPTO_4866
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001921141 – 534Bifunctional purine biosynthesis protein PurHAdd BLAST534

Interactioni

Protein-protein interaction databases

STRINGi223283.PSPTO_4866

Structurei

3D structure databases

ProteinModelPortaliQ87VR9
SMRiQ87VR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 151MGS-likePROSITE-ProRule annotationAdd BLAST146

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DC1 Bacteria
COG0138 LUCA
HOGENOMiHOG000230373
KOiK00602
OMAiDLLFAWK
OrthoDBiPOG091H00UT
PhylomeDBiQ87VR9

Family and domain databases

Gene3Di3.40.140.20, 2 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q87VR9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDQTTRLPI RRALISVSDK TGILEFAREL EALGVEILST GGTFKLLQDN
60 70 80 90 100
GVAAVEVADY TGFAEMMDGR VKTLHPKIHG GILGRRGIDD AIMNEHGIKP
110 120 130 140 150
IDLVAVNLYP FEATVSKPGC DLPTAIENID IGGPTMVRSA AKNHKDVAIV
160 170 180 190 200
VNAGDYGQVL ESLKAGGLTY AQRFDLMLKA FEHTAAYDGM IANYLGGVDQ
210 220 230 240 250
TADTLSTEGR SQFPRTFNTQ FVKAQEMRYG ENPHQSAAFY VEAKPAEVGI
260 270 280 290 300
ATATQLQGKE LSFNNVADTD AALECVKSFV KPACVIVKHA NPCGVAVCPD
310 320 330 340 350
DEGGIRQAYE LAYATDSESA FGGIIAFNRE LDAATAKAIV ERQFVEVIIA
360 370 380 390 400
PSVSAEARSI IASKANVRLL TSGQWSERLP AWDYKRVNGG LLVQSRDIGM
410 420 430 440 450
ITEADLKVVT QRAPTEHEMH DLIFAWKVAK YVKSNAIVYA RNRQTIGVGA
460 470 480 490 500
GQMSRVNSAR IAAIKAEHAG LQVQGAVMAS DAFFPFRDGI DNAAKVGISA
510 520 530
VIQPGGSMRD NEVIAAADEA GIAMVFTGMR HFRH
Length:534
Mass (Da):57,463
Last modified:June 20, 2003 - v1
Checksum:i002B14FE9EF285E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016853 Genomic DNA Translation: AAO58295.1
RefSeqiNP_794600.1, NC_004578.1
WP_011105209.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO58295; AAO58295; PSPTO_4866
GeneIDi1186549
KEGGipst:PSPTO_4866
PATRICifig|223283.9.peg.4978

Similar proteinsi

Entry informationi

Entry nameiPUR9_PSESM
AccessioniPrimary (citable) accession number: Q87VR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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