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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP; via amide nitrogenUniRule annotation1
Metal bindingi104Magnesium; catalyticUniRule annotation1
Active sitei128Proton acceptorUniRule annotation1
Binding sitei155Allosteric activator ADPUniRule annotation1
Binding sitei163Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei212Allosteric activator ADPUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Binding sitei244Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 74ATPUniRule annotation2
Nucleotide bindingi103 – 106ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-2961-MONOMER
UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:VP2855
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120061 – 320ATP-dependent 6-phosphofructokinaseAdd BLAST320

Proteomic databases

PRIDEiQ87KX0

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi223926.VP2855

Structurei

3D structure databases

ProteinModelPortaliQ87KX0
SMRiQ87KX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 26Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni126 – 128Substrate bindingUniRule annotation3
Regioni170 – 172Substrate bindingUniRule annotation3
Regioni186 – 188Allosteric activator ADP bindingUniRule annotation3
Regioni214 – 216Allosteric activator ADP bindingUniRule annotation3
Regioni250 – 253Substrate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiRDGWRGP

Family and domain databases

CDDicd00763 Bacterial_PFK, 1 hit
HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q87KX0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKKIGVLTS GGDAPGMNAA IRGVVRTALS EGLEVFGVYD GYLGLYEGRI
60 70 80 90 100
EKLDRSSVSD VINKGGTFLG SARFPEFKQV EVREKAIENL KKHGIDALVV
110 120 130 140 150
IGGDGSYMGA KKLTEMGYPC IGLPGTIDND IAGTDYTVGY LSALNTVIDA
160 170 180 190 200
IDRLRDTSSS HQRISIVEIM GRHCGDLTLM SAIAGGCEYI ITPETGLDKD
210 220 230 240 250
KLISNIQDGI AKGKKHAIIA LTELMMDANE LARDIEAATG RETRATVLGH
260 270 280 290 300
IQRGGRPTAF DRVLASRMGN YAVHLLLEGH GGRCVGIVKE QLVHHDIIDA
310 320
IENMKRPVRN DLYKVAEELF
Length:320
Mass (Da):34,583
Last modified:June 16, 2003 - v1
Checksum:iA39DEF12E37AF211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA Translation: BAC61118.1
RefSeqiNP_799234.1, NC_004603.1
WP_005460467.1, NC_004603.1

Genome annotation databases

EnsemblBacteriaiBAC61118; BAC61118; BAC61118
GeneIDi1190418
KEGGivpa:VP2855
PATRICifig|223926.6.peg.2747

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA Translation: BAC61118.1
RefSeqiNP_799234.1, NC_004603.1
WP_005460467.1, NC_004603.1

3D structure databases

ProteinModelPortaliQ87KX0
SMRiQ87KX0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP2855

Proteomic databases

PRIDEiQ87KX0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC61118; BAC61118; BAC61118
GeneIDi1190418
KEGGivpa:VP2855
PATRICifig|223926.6.peg.2747

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiRDGWRGP

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

BioCyciVPAR223926:G1GTB-2961-MONOMER

Family and domain databases

CDDicd00763 Bacterial_PFK, 1 hit
HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_VIBPA
AccessioniPrimary (citable) accession number: Q87KX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: October 10, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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