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Protein

Glutamine-dependent NAD(+) synthetase

Gene

nadE

Organism
Xylella fastidiosa (strain Temecula1 / ATCC 700964)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei46Proton acceptor; for glutaminase activityUniRule annotation1
Active sitei113For glutaminase activityUniRule annotation1
Binding sitei119L-glutamineUniRule annotation1
Active sitei151Nucleophile; for glutaminase activityUniRule annotation1
Binding sitei177L-glutamineUniRule annotation1
Binding sitei183L-glutamineUniRule annotation1
Binding sitei375Deamido-NADUniRule annotation1
Binding sitei399ATPUniRule annotation1
Binding sitei404Deamido-NADUniRule annotation1
Binding sitei516Deamido-NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 299ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.5.1UniRule annotation)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]UniRule annotation
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:PD_0839
OrganismiXylella fastidiosa (strain Temecula1 / ATCC 700964)
Taxonomic identifieri183190 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella
Proteomesi
  • UP000002516 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003064141 – 545Glutamine-dependent NAD(+) synthetaseAdd BLAST545

Structurei

3D structure databases

ProteinModelPortaliQ87D47
SMRiQ87D47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 247CN hydrolasePROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 545LigaseAdd BLAST277

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.UniRule annotationCurated

Phylogenomic databases

KOiK01950
OMAiCEDIWND

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.110.10, 1 hit
HAMAPiMF_02090 NadE_glutamine_dep, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR000132 Nitrilase/CN_hydratase_CS
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit
PIRSFiPIRSF006630 NADS_GAT, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00920 NITRIL_CHT_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q87D47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFLRIAMA QFDFPVGAVA QNAERIIALI EQARDEHGAD VVMFPELALS
60 70 80 90 100
GYPPEDLLLR PGFLAHCQVA IERIAAATHG IVAVVGWPQS AGSVVYNVAS
110 120 130 140 150
VLCDGQVEQT YRKRELPNYA VFDERRYFEV DPNGSRCVFK VKGVPVGVLI
160 170 180 190 200
CEDLWFSEPL ADTVCGGAEL VLVPNASPYE RGKHAQRDAL LAERARETGA
210 220 230 240 250
AIAYLNVVGG QDALVFDGAS VVVDGHGRVH PAAAAFSDQW LVVDYMRSER
260 270 280 290 300
RFVPLQWVVE SEVSINALVW RAVVRGVQDY CRKNGFSKVW VGLSGGIDSA
310 320 330 340 350
LVLAIAVDAL GADQVTAVRL PSRYTAELSN DLAAEQCHSL GVRLETVAIE
360 370 380 390 400
PVFEGLLAAL GPLFAGMAPD ATEENLQSRS RGVILMALAN KFGGLLLTTG
410 420 430 440 450
NKSEYAVGYA TIYGDMCGGY APLKDIYKSQ VFELAQWRNR VSDVLAIPPG
460 470 480 490 500
VIHRPPSAEL RAQQTDQDSL PPYEVLDGIL SLYVDQEQSR EDIIAAGYAA
510 520 530 540
GVVDYVLNLV RINEWKRHQA APGPKVSQRA FGRERRYPIS NAYRG
Length:545
Mass (Da):59,373
Last modified:June 1, 2003 - v1
Checksum:i5F4E1CC3F34F0420
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009442 Genomic DNA Translation: AAO28707.1
RefSeqiWP_004572843.1, NC_004556.1

Genome annotation databases

EnsemblBacteriaiAAO28707; AAO28707; PD_0839
KEGGixft:PD_0839

Similar proteinsi

Entry informationi

Entry nameiNADE_XYLFT
AccessioniPrimary (citable) accession number: Q87D47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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