Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inactive tyrosine-protein kinase PRAG1

Gene

PRAG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (By similarity).By similarity

Caution

Despite of the presence of a putative ATP-binding motif, this protein does not bind ATP, suggesting that it has no protein kinase activity.By similarity

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • protein kinase activity Source: GO_Central

GO - Biological processi

Enzyme and pathway databases

SignaLinkiQ86YV5

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive tyrosine-protein kinase PRAG1Curated
Alternative name(s):
PEAK1-related kinase-activating pseudokinase 1
Pragmin1 Publication
Sugen kinase 2231 Publication
Short name:
SgK223
Gene namesi
Name:PRAG1Imported
Synonyms:SGK223
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000275342.4
HGNCiHGNC:25438 PRAG1
MIMi617344 gene
neXtProtiNX_Q86YV5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi955L → A: Decreases homodimerization. Abolished interaction with PEAK1. No effect on cell migration. 1 Publication1
Mutagenesisi966L → A: Decreases homodimerization. Abolished interaction with PEAK1. Decreases cell migration. 1 Publication1
Mutagenesisi1243I → A: No effect on homodimerization. Decreases oligomerization. Decreases interaction with PEAK1. 1 Publication1
Mutagenesisi1271F → A: Decreases homodimerization. Decreases interaction with PEAK1. 1 Publication1
Mutagenesisi1278R → A: Decreases homodimerization. 1 Publication1
Mutagenesisi1282Y → A: No effect on homodimerization. Decreases oligomerization. Decreases interaction with PEAK1. No effect on cell migration. 1 Publication1
Mutagenesisi1366F → A: Decreases homodimerization. Abolished interaction with PEAK1. Decreases cell migration. 1 Publication1
Mutagenesisi1382W → A: Decreases homodimerization. Abolished interaction with PEAK1. Decreases cell migration. 1 Publication1

Organism-specific databases

DisGeNETi157285
OpenTargetsiENSG00000275342

Polymorphism and mutation databases

BioMutaiSGK223
DMDMi327478560

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002630081 – 1406Inactive tyrosine-protein kinase PRAG1Add BLAST1406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei148PhosphoserineCombined sources1
Modified residuei253PhosphotyrosineBy similarity1
Modified residuei365PhosphotyrosineBy similarity1
Modified residuei413PhosphotyrosineBy similarity1
Modified residuei696PhosphoserineCombined sources1
Modified residuei745PhosphoserineCombined sources1
Modified residuei782PhosphoserineCombined sources1
Modified residuei826PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CSK on Tyr-253, Tyr-365, and Tyr-413; Tyr-413 is a primary site of phosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86YV5
PaxDbiQ86YV5
PeptideAtlasiQ86YV5
PRIDEiQ86YV5
ProteomicsDBi70475

PTM databases

iPTMnetiQ86YV5
PhosphoSitePlusiQ86YV5

Expressioni

Gene expression databases

BgeeiENSG00000275342 Expressed in 199 organ(s), highest expression level in cerebellar vermis
GenevisibleiQ86YV5 HS

Organism-specific databases

HPAiHPA012066

Interactioni

Subunit structurei

Homodimer (PubMed:29079850). Dimerization leads to the catalytic activation of CSK (By similarity). Interacts (via C-terminus) with RND2 (By similarity). Interacts with CSK (via SH2 domain) in a Tyr-413 phosphorylation-dependent manner; this interaction potentiates kinase activity of CSK (By similarity). Interacts with PEAK1 (PubMed:29079850). Interacts with NOTCH1 intracellular domain (N1ICD) (By similarity). Forms a complex with N1ICD and MAML1, in a MAML1-dependent manner (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi127591, 6 interactors
IntActiQ86YV5, 7 interactors
MINTiQ86YV5
STRINGi9606.ENSP00000330930

Structurei

Secondary structure

11406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ86YV5
SMRiQ86YV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini978 – 1329Protein kinasePROSITE-ProRule annotationAdd BLAST352

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni933 – 976Required for homodimerizationBy similarityAdd BLAST44
Regioni1331 – 1406Required for homodimerizationBy similarityAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 58Poly-Pro4
Compositional biasi713 – 718Poly-Pro6

Domaini

The dimerization region encompasses helices both from the N- and C-terminal of the protein kinase domain.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410IJMD Eukaryota
ENOG4110SRP LUCA
GeneTreeiENSGT00460000041554
HOGENOMiHOG000168455
HOVERGENiHBG055817
InParanoidiQ86YV5
KOiK17537
OMAiVHDESHF
OrthoDBiEOG091G03K2

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008266 Tyr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequencei

Sequence statusi: Complete.

Q86YV5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHQTLCLNPE SLKMSACSDF VEHIWKPGSC KNCFCLRSDH QLVAGPPQPR
60 70 80 90 100
AGSLPPPPRL PPRPENCRLE DEGVNSSPYS KPTIAVKPTM MSSEASDVWT
110 120 130 140 150
EANLSAEVSQ VIWRRAPGKL PLPKQEDAPV VYLGSFRGVQ KPAGPSTSPD
160 170 180 190 200
GNSRCPPAYT MVGLHNLEPR GERNIAFHPV SFPEEKAVHK EKPSFPYQDR
210 220 230 240 250
PSTQESFRQK LAAFAGTTSG CHQGPGPLRE SLPSEDDSDQ RCSPSGDSEG
260 270 280 290 300
GEYCSILDCC PGSPVAKAAS QTAGSRGRHG GRDCSPTCWE QGKCSGPAEQ
310 320 330 340 350
EKRGPSFPKE CCSQGPTAHP SCLGPKKLSL TSEAAISSDG LSCGSGSGSG
360 370 380 390 400
SGASSPFVPH LESDYCSLMK EPAPEKQQDP GCPGVTPSRC LGLTGEPQPP
410 420 430 440 450
AHPREATQPE PIYAESTKRK KAAPVPSKSQ AKIEHAAAAQ GQGQVCTGNA
460 470 480 490 500
WAQKAASGWG RDSPDPTPQV SATITVMAAH PEEDHRTIYL SSPDSAVGVQ
510 520 530 540 550
WPRGPVSQNS EVGEEETSAG QGLSSRESHA HSASESKPKE RPAIPPKLSK
560 570 580 590 600
SSPVGSPVSP SAGGPPVSPL ADLSDGSSGG SSIGPQPPSQ GPADPAPSCR
610 620 630 640 650
TNGVAISDPS RCPQPAASSA SEQRRPRFQA GTWSRQCRIE EEEEVEQELL
660 670 680 690 700
SHSWGRETKN GPTDHSNSTT WHRLHPTDGS SGQNSKVGTG MSKSASFAFE
710 720 730 740 750
FPKDRSGIET FSPPPPPPKS RHLLKMNKSS SDLEKVSQGS AESLSPSFRG
760 770 780 790 800
VHVSFTTGST DSLASDSRTC SDGGPSSELA HSPTNSGKKL FAPVPFPSGS
810 820 830 840 850
TEDVSPSGPQ QPPPLPQKKI VSRAASSPDG FFWTQGSPKP GTASPKLNLS
860 870 880 890 900
HSETNVHDES HFSYSLSPGN RHHPVFSSSD PLEKAFKGSG HWLPAAGLAG
910 920 930 940 950
NRGGCGSPGL QCKGAPSASS SQLSVSSQAS TGSTQLQLHG LLSNISSKEG
960 970 980 990 1000
TYAKLGGLYT QSLARLVAKC EDLFMGGQKK ELHFNENNWS LFKLTCNKPC
1010 1020 1030 1040 1050
CDSGDAIYYC ATCSEDPGST YAVKICKAPE PKTVSYCSPS VPVHFNIQQD
1060 1070 1080 1090 1100
CGHFVASVPS SMLSSPDAPK DPVPALPTHP PAQEQDCVVV ITREVPHQTA
1110 1120 1130 1140 1150
SDFVRDSAAS HQAEPEAYER RVCFLLLQLC NGLEHLKEHG IIHRDLCLEN
1160 1170 1180 1190 1200
LLLVHCTLQA GPGPAPAPAP APAPAAAAPP CSSAAPPAGG TLSPAAGPAS
1210 1220 1230 1240 1250
PEGPREKQLP RLIISNFLKA KQKPGGTPNL QQKKSQARLA PEIVSASQYR
1260 1270 1280 1290 1300
KFDEFQTGIL IYELLHQPNP FEVRAQLRER DYRQEDLPPL PALSLYSPGL
1310 1320 1330 1340 1350
QQLAHLLLEA DPIKRIRIGE AKRVLQCLLW GPRRELVQQP GTSEEALCGT
1360 1370 1380 1390 1400
LHNWIDMKRA LMMMKFAEKA VDRRRGVELE DWLCCQYLAS AEPGALLQSL

KLLQLL
Length:1,406
Mass (Da):149,624
Last modified:October 1, 2014 - v4
Checksum:i941BCF315B826148
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1226G → S in BAC56923 (Ref. 2) Curated1
Sequence conflicti1226G → S in CAD38729 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041803122L → I1 PublicationCorresponds to variant dbSNP:rs55764617Ensembl.1
Natural variantiVAR_041804137R → G1 PublicationCorresponds to variant dbSNP:rs56290960Ensembl.1
Natural variantiVAR_041805139V → I1 PublicationCorresponds to variant dbSNP:rs34346032Ensembl.1
Natural variantiVAR_041806404R → Q2 PublicationsCorresponds to variant dbSNP:rs3896980Ensembl.1
Natural variantiVAR_041807569P → L2 PublicationsCorresponds to variant dbSNP:rs4840955Ensembl.1
Natural variantiVAR_041808578S → C2 PublicationsCorresponds to variant dbSNP:rs4840953Ensembl.1
Natural variantiVAR_041809595P → A1 PublicationCorresponds to variant dbSNP:rs55994745Ensembl.1
Natural variantiVAR_041810662P → T1 PublicationCorresponds to variant dbSNP:rs56351643Ensembl.1
Natural variantiVAR_041811814P → L1 PublicationCorresponds to variant dbSNP:rs56207906Ensembl.1
Natural variantiVAR_041812851H → R1 PublicationCorresponds to variant dbSNP:rs56215812Ensembl.1
Natural variantiVAR_0418131003S → L1 PublicationCorresponds to variant dbSNP:rs56289289Ensembl.1
Natural variantiVAR_0418141041V → M1 PublicationCorresponds to variant dbSNP:rs28533138Ensembl.1
Natural variantiVAR_0418151113A → T3 PublicationsCorresponds to variant dbSNP:rs12549973Ensembl.1
Natural variantiVAR_0418161315R → H1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068353 Genomic DNA No translation available.
AC103957 Genomic DNA No translation available.
AK122582 mRNA Translation: BAC56923.1
AL833872 mRNA Translation: CAD38729.1
CCDSiCCDS43706.1
RefSeqiNP_001074295.2, NM_001080826.2
XP_005272426.2, XM_005272369.4
XP_005272427.2, XM_005272370.4
UniGeneiHs.583863
Hs.657673

Genome annotation databases

EnsembliENST00000615670; ENSP00000481109; ENSG00000275342
ENST00000622241; ENSP00000479068; ENSG00000275342
GeneIDi157285
KEGGihsa:157285
UCSCiuc064kbu.1 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068353 Genomic DNA No translation available.
AC103957 Genomic DNA No translation available.
AK122582 mRNA Translation: BAC56923.1
AL833872 mRNA Translation: CAD38729.1
CCDSiCCDS43706.1
RefSeqiNP_001074295.2, NM_001080826.2
XP_005272426.2, XM_005272369.4
XP_005272427.2, XM_005272370.4
UniGeneiHs.583863
Hs.657673

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VE6X-ray2.95A932-1406[»]
ProteinModelPortaliQ86YV5
SMRiQ86YV5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127591, 6 interactors
IntActiQ86YV5, 7 interactors
MINTiQ86YV5
STRINGi9606.ENSP00000330930

PTM databases

iPTMnetiQ86YV5
PhosphoSitePlusiQ86YV5

Polymorphism and mutation databases

BioMutaiSGK223
DMDMi327478560

Proteomic databases

EPDiQ86YV5
PaxDbiQ86YV5
PeptideAtlasiQ86YV5
PRIDEiQ86YV5
ProteomicsDBi70475

Protocols and materials databases

DNASUi157285
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000615670; ENSP00000481109; ENSG00000275342
ENST00000622241; ENSP00000479068; ENSG00000275342
GeneIDi157285
KEGGihsa:157285
UCSCiuc064kbu.1 human

Organism-specific databases

CTDi157285
DisGeNETi157285
EuPathDBiHostDB:ENSG00000275342.4
GeneCardsiPRAG1
H-InvDBiHIX0007293
HGNCiHGNC:25438 PRAG1
HPAiHPA012066
MIMi617344 gene
neXtProtiNX_Q86YV5
OpenTargetsiENSG00000275342
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJMD Eukaryota
ENOG4110SRP LUCA
GeneTreeiENSGT00460000041554
HOGENOMiHOG000168455
HOVERGENiHBG055817
InParanoidiQ86YV5
KOiK17537
OMAiVHDESHF
OrthoDBiEOG091G03K2

Enzyme and pathway databases

SignaLinkiQ86YV5

Miscellaneous databases

ChiTaRSiSGK223 human
GenomeRNAii157285
PROiPR:Q86YV5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000275342 Expressed in 199 organ(s), highest expression level in cerebellar vermis
GenevisibleiQ86YV5 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008266 Tyr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRAG1_HUMAN
AccessioniPrimary (citable) accession number: Q86YV5
Secondary accession number(s): Q8N3N5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2014
Last modified: November 7, 2018
This is version 135 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again