UniProtKB - Q86YS6 (RAB43_HUMAN)
Ras-related protein Rab-43
RAB43
Functioni
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 32 | GTP | 8 | |
Nucleotide bindingi | 73 – 77 | GTPBy similarity | 5 | |
Nucleotide bindingi | 131 – 134 | GTP | 4 | |
Nucleotide bindingi | 163 – 164 | GTP | 2 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- autophagosome assembly Source: GO_Central
- cellular response to interferon-gamma Source: Ensembl
- Golgi organization Source: UniProtKB
- intracellular protein transport Source: GO_Central
- phagosome maturation Source: UniProtKB
- retrograde transport, plasma membrane to Golgi Source: UniProtKB
- toxin transport Source: Ensembl
- virion assembly Source: UniProtKB
Keywordsi
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q86YS6 |
Reactomei | R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8873719, RAB geranylgeranylation |
SignaLinki | Q86YS6 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-43Alternative name(s): Ras-related protein Rab-41 |
Gene namesi | Name:RAB43 Synonyms:RAB41 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19983, RAB43 |
neXtProti | NX_Q86YS6 |
VEuPathDBi | HostDB:ENSG00000172780 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 2 Publications
- trans-Golgi network membrane Curated; Lipid-anchor Curated
- trans-Golgi network 1 Publication
Other locations
- phagosome 1 Publication
- phagosome membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network membrane Source: Reactome
Other locations
- endomembrane system Source: GO_Central
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 32 | T → N: Abolishes activity. Disrupts Golgi structure. 2 Publications | 1 | |
Mutagenesisi | 82 | T → A: Loss of phosphorylation. No effect on binding of GDI1 and GDI2. 1 Publication | 1 | |
Mutagenesisi | 82 | T → E: Phosphomimetic mutant. Loss of binding to GDI1, GDI2, CHM and CHML. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 339122 |
OpenTargetsi | ENSG00000172780 |
PharmGKBi | PA134968262 |
Miscellaneous databases
Pharosi | Q86YS6, Tbio |
Genetic variation databases
BioMutai | RAB43 |
DMDMi | 74727944 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000244615 | 1 – 212 | Ras-related protein Rab-43Add BLAST | 212 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 82 | Phosphothreonine; by LRRK21 Publication | 1 | |
Modified residuei | 193 | PhosphoserineCombined sources | 1 | |
Lipidationi | 210 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 212 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 212 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | Q86YS6 |
jPOSTi | Q86YS6 |
MassIVEi | Q86YS6 |
MaxQBi | Q86YS6 |
PaxDbi | Q86YS6 |
PeptideAtlasi | Q86YS6 |
PRIDEi | Q86YS6 |
ProteomicsDBi | 19270 70463 [Q86YS6-1] |
PTM databases
iPTMneti | Q86YS6 |
PhosphoSitePlusi | Q86YS6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000172780, Expressed in blood and 111 other tissues |
ExpressionAtlasi | Q86YS6, baseline and differential |
Genevisiblei | Q86YS6, HS |
Organism-specific databases
HPAi | ENSG00000172780, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Interacts with GDI1, GDI2, CHM and CHML; phosphorylation at Thr-82 disrupts these interactions.
1 PublicationBinary interactionsi
Q86YS6
With | #Exp. | IntAct |
---|---|---|
HSPB1 [P04792] | 2 | EBI-4401730,EBI-352682 |
Protein-protein interaction databases
BioGRIDi | 130830, 41 interactors |
IntActi | Q86YS6, 6 interactors |
MINTi | Q86YS6 |
STRINGi | 9606.ENSP00000319781 |
Miscellaneous databases
RNActi | Q86YS6, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q86YS6 |
SMRi | Q86YS6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q86YS6 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 47 – 55 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0084, Eukaryota |
GeneTreei | ENSGT00940000161980 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | Q86YS6 |
OMAi | MFVMETS |
PhylomeDBi | Q86YS6 |
TreeFami | TF300097 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGPGPGPGD PDEQYDFLFK LVLVGDASVG KTCVVQRFKT GAFSERQGST
60 70 80 90 100
IGVDFTMKTL EIQGKRVKLQ IWDTAGQERF RTITQSYYRS ANGAILAYDI
110 120 130 140 150
TKRSSFLSVP HWIEDVRKYA GSNIVQLLIG NKSDLSELRE VSLAEAQSLA
160 170 180 190 200
EHYDILCAIE TSAKDSSNVE EAFLRVATEL IMRHGGPLFS EKSPDHIQLN
210
SKDIGEGWGC GC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JFM7 | C9JFM7_HUMAN | Ras-related protein Rab-43 | RAB43 | 86 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054030 | 130 – 155 | GNKSD…EHYDI → EMQSCYVAQADLELLASSNP PASTSK in isoform 3. CuratedAdd BLAST | 26 | |
Alternative sequenceiVSP_054031 | 156 – 212 | Missing in isoform 3. CuratedAdd BLAST | 57 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166852 mRNA Translation: AAO17291.1 AK291014 mRNA Translation: BAF83703.1 AC108673 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79283.1 BC062319 mRNA Translation: AAH62319.1 |
CCDSi | CCDS33850.1 [Q86YS6-1] CCDS56275.1 [Q86YS6-2] |
RefSeqi | NP_001191812.1, NM_001204883.1 [Q86YS6-1] NP_001191813.1, NM_001204884.1 [Q86YS6-1] NP_001191814.1, NM_001204885.1 [Q86YS6-1] NP_001191815.1, NM_001204886.1 [Q86YS6-1] NP_001191816.1, NM_001204887.1 [Q86YS6-2] NP_940892.1, NM_198490.2 [Q86YS6-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166852 mRNA Translation: AAO17291.1 AK291014 mRNA Translation: BAF83703.1 AC108673 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79283.1 BC062319 mRNA Translation: AAH62319.1 |
CCDSi | CCDS33850.1 [Q86YS6-1] CCDS56275.1 [Q86YS6-2] |
RefSeqi | NP_001191812.1, NM_001204883.1 [Q86YS6-1] NP_001191813.1, NM_001204884.1 [Q86YS6-1] NP_001191814.1, NM_001204885.1 [Q86YS6-1] NP_001191815.1, NM_001204886.1 [Q86YS6-1] NP_001191816.1, NM_001204887.1 [Q86YS6-2] NP_940892.1, NM_198490.2 [Q86YS6-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HUP | X-ray | 2.05 | A/B | 9-190 | [»] | |
AlphaFoldDBi | Q86YS6 | |||||
SMRi | Q86YS6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 130830, 41 interactors |
IntActi | Q86YS6, 6 interactors |
MINTi | Q86YS6 |
STRINGi | 9606.ENSP00000319781 |
PTM databases
iPTMneti | Q86YS6 |
PhosphoSitePlusi | Q86YS6 |
Genetic variation databases
BioMutai | RAB43 |
DMDMi | 74727944 |
Proteomic databases
EPDi | Q86YS6 |
jPOSTi | Q86YS6 |
MassIVEi | Q86YS6 |
MaxQBi | Q86YS6 |
PaxDbi | Q86YS6 |
PeptideAtlasi | Q86YS6 |
PRIDEi | Q86YS6 |
ProteomicsDBi | 19270 70463 [Q86YS6-1] |
Protocols and materials databases
Antibodypediai | 34842, 100 antibodies from 24 providers |
DNASUi | 339122 |
Genome annotation databases
Organism-specific databases
CTDi | 339122 |
DisGeNETi | 339122 |
GeneCardsi | RAB43 |
HGNCi | HGNC:19983, RAB43 |
HPAi | ENSG00000172780, Tissue enhanced (liver) |
neXtProti | NX_Q86YS6 |
OpenTargetsi | ENSG00000172780 |
PharmGKBi | PA134968262 |
VEuPathDBi | HostDB:ENSG00000172780 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0084, Eukaryota |
GeneTreei | ENSGT00940000161980 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | Q86YS6 |
OMAi | MFVMETS |
PhylomeDBi | Q86YS6 |
TreeFami | TF300097 |
Enzyme and pathway databases
PathwayCommonsi | Q86YS6 |
Reactomei | R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8873719, RAB geranylgeranylation |
SignaLinki | Q86YS6 |
Miscellaneous databases
BioGRID-ORCSi | 339122, 41 hits in 1041 CRISPR screens |
EvolutionaryTracei | Q86YS6 |
GenomeRNAii | 339122 |
Pharosi | Q86YS6, Tbio |
PROi | PR:Q86YS6 |
RNActi | Q86YS6, protein |
Gene expression databases
Bgeei | ENSG00000172780, Expressed in blood and 111 other tissues |
ExpressionAtlasi | Q86YS6, baseline and differential |
Genevisiblei | Q86YS6, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB43_HUMAN | |
Accessioni | Q86YS6Primary (citable) accession number: Q86YS6 Secondary accession number(s): A8K4P9, E9PBQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families