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Protein

Ras-related protein Rab-43

Gene

RAB43

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32GTP8
Nucleotide bindingi73 – 77GTPBy similarity5
Nucleotide bindingi131 – 134GTP4
Nucleotide bindingi163 – 164GTP2

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
R-HSA-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-43
Alternative name(s):
Ras-related protein Rab-41
Gene namesi
Name:RAB43
Synonyms:RAB41
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000172780.16
HGNCiHGNC:19983 RAB43
neXtProtiNX_Q86YS6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32T → N: Abolishes activity. Disrupts Golgi structure. 2 Publications1

Organism-specific databases

OpenTargetsiENSG00000172780
PharmGKBiPA134968262

Polymorphism and mutation databases

BioMutaiRAB43
DMDMi74727944

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002446151 – 212Ras-related protein Rab-43Add BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1
Modified residuei193PhosphoserineCombined sources1
Lipidationi210S-geranylgeranyl cysteineBy similarity1
Modified residuei212Cysteine methyl esterBy similarity1
Lipidationi212S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ86YS6
MaxQBiQ86YS6
PaxDbiQ86YS6
PeptideAtlasiQ86YS6
PRIDEiQ86YS6
ProteomicsDBi70463

PTM databases

iPTMnetiQ86YS6
PhosphoSitePlusiQ86YS6

Expressioni

Tissue specificityi

Widely expressed in brain, testis, lung, heart, ovary, colon, kidney, uterus and spleen but not in liver.1 Publication

Gene expression databases

BgeeiENSG00000172780 Expressed in 84 organ(s), highest expression level in blood
CleanExiHS_RAB41
HS_RAB43
ExpressionAtlasiQ86YS6 baseline and differential
GenevisibleiQ86YS6 HS

Organism-specific databases

HPAiHPA046840
HPA060085

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPB1P047922EBI-4401730,EBI-352682

Protein-protein interaction databases

BioGridi130830, 31 interactors
IntActiQ86YS6, 5 interactors
STRINGi9606.ENSP00000319781

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ86YS6
SMRiQ86YS6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86YS6

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 55Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084 Eukaryota
ENOG410XQN5 LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ86YS6
KOiK07930
OMAiMFVMETS
OrthoDBiEOG091G0HS5
PhylomeDBiQ86YS6
TreeFamiTF300097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86YS6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGPGPGPGD PDEQYDFLFK LVLVGDASVG KTCVVQRFKT GAFSERQGST
60 70 80 90 100
IGVDFTMKTL EIQGKRVKLQ IWDTAGQERF RTITQSYYRS ANGAILAYDI
110 120 130 140 150
TKRSSFLSVP HWIEDVRKYA GSNIVQLLIG NKSDLSELRE VSLAEAQSLA
160 170 180 190 200
EHYDILCAIE TSAKDSSNVE EAFLRVATEL IMRHGGPLFS EKSPDHIQLN
210
SKDIGEGWGC GC
Length:212
Mass (Da):23,339
Last modified:June 1, 2003 - v1
Checksum:i087EE3802B7159C9
GO
Isoform 2 (identifier: Q9ULR0-1) [UniParc]FASTAAdd to basket
Also known as: ISY1-RAB43
The sequence of this isoform can be found in the external entry Q9ULR0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a readthrough transcript which may produce a ISY1-RAB43 fusion protein. No experimental confirmation available.
Length:331
Mass (Da):37,567
GO
Isoform 3 (identifier: Q86YS6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-155: GNKSDLSELREVSLAEAQSLAEHYDI → EMQSCYVAQADLELLASSNPPASTSK
     156-212: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:155
Mass (Da):17,074
Checksum:iCC4EA3C8FD5E005D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JFM7C9JFM7_HUMAN
Ras-related protein Rab-43
RAB43
86Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054030130 – 155GNKSD…EHYDI → EMQSCYVAQADLELLASSNP PASTSK in isoform 3. CuratedAdd BLAST26
Alternative sequenceiVSP_054031156 – 212Missing in isoform 3. CuratedAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY166852 mRNA Translation: AAO17291.1
AK291014 mRNA Translation: BAF83703.1
AC108673 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79283.1
BC062319 mRNA Translation: AAH62319.1
CCDSiCCDS33850.1 [Q86YS6-1]
CCDS56275.1 [Q86YS6-2]
RefSeqiNP_001191812.1, NM_001204883.1 [Q86YS6-1]
NP_001191813.1, NM_001204884.1 [Q86YS6-1]
NP_001191814.1, NM_001204885.1 [Q86YS6-1]
NP_001191815.1, NM_001204886.1 [Q86YS6-1]
NP_001191816.1, NM_001204887.1 [Q86YS6-2]
NP_940892.1, NM_198490.2 [Q86YS6-1]
UniGeneiHs.381132
Hs.512661
Hs.546542

Genome annotation databases

EnsembliENST00000315150; ENSP00000319781; ENSG00000172780 [Q86YS6-1]
ENST00000393304; ENSP00000376981; ENSG00000172780 [Q86YS6-1]
ENST00000393305; ENSP00000376982; ENSG00000172780 [Q86YS6-1]
ENST00000393307; ENSP00000376984; ENSG00000172780 [Q86YS6-1]
ENST00000393308; ENSP00000376985; ENSG00000172780 [Q86YS6-1]
ENST00000476465; ENSP00000427632; ENSG00000172780 [Q86YS6-2]
ENST00000615093; ENSP00000481399; ENSG00000172780 [Q86YS6-2]
GeneIDi339122
KEGGihsa:339122
UCSCiuc003eln.3 human [Q86YS6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY166852 mRNA Translation: AAO17291.1
AK291014 mRNA Translation: BAF83703.1
AC108673 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79283.1
BC062319 mRNA Translation: AAH62319.1
CCDSiCCDS33850.1 [Q86YS6-1]
CCDS56275.1 [Q86YS6-2]
RefSeqiNP_001191812.1, NM_001204883.1 [Q86YS6-1]
NP_001191813.1, NM_001204884.1 [Q86YS6-1]
NP_001191814.1, NM_001204885.1 [Q86YS6-1]
NP_001191815.1, NM_001204886.1 [Q86YS6-1]
NP_001191816.1, NM_001204887.1 [Q86YS6-2]
NP_940892.1, NM_198490.2 [Q86YS6-1]
UniGeneiHs.381132
Hs.512661
Hs.546542

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HUPX-ray2.05A/B9-190[»]
ProteinModelPortaliQ86YS6
SMRiQ86YS6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130830, 31 interactors
IntActiQ86YS6, 5 interactors
STRINGi9606.ENSP00000319781

PTM databases

iPTMnetiQ86YS6
PhosphoSitePlusiQ86YS6

Polymorphism and mutation databases

BioMutaiRAB43
DMDMi74727944

Proteomic databases

EPDiQ86YS6
MaxQBiQ86YS6
PaxDbiQ86YS6
PeptideAtlasiQ86YS6
PRIDEiQ86YS6
ProteomicsDBi70463

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315150; ENSP00000319781; ENSG00000172780 [Q86YS6-1]
ENST00000393304; ENSP00000376981; ENSG00000172780 [Q86YS6-1]
ENST00000393305; ENSP00000376982; ENSG00000172780 [Q86YS6-1]
ENST00000393307; ENSP00000376984; ENSG00000172780 [Q86YS6-1]
ENST00000393308; ENSP00000376985; ENSG00000172780 [Q86YS6-1]
ENST00000476465; ENSP00000427632; ENSG00000172780 [Q86YS6-2]
ENST00000615093; ENSP00000481399; ENSG00000172780 [Q86YS6-2]
GeneIDi339122
KEGGihsa:339122
UCSCiuc003eln.3 human [Q86YS6-1]

Organism-specific databases

CTDi339122
EuPathDBiHostDB:ENSG00000172780.16
GeneCardsiRAB43
HGNCiHGNC:19983 RAB43
HPAiHPA046840
HPA060085
neXtProtiNX_Q86YS6
OpenTargetsiENSG00000172780
PharmGKBiPA134968262
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0084 Eukaryota
ENOG410XQN5 LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ86YS6
KOiK07930
OMAiMFVMETS
OrthoDBiEOG091G0HS5
PhylomeDBiQ86YS6
TreeFamiTF300097

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
R-HSA-8873719 RAB geranylgeranylation

Miscellaneous databases

EvolutionaryTraceiQ86YS6
GenomeRNAii339122
PROiPR:Q86YS6

Gene expression databases

BgeeiENSG00000172780 Expressed in 84 organ(s), highest expression level in blood
CleanExiHS_RAB41
HS_RAB43
ExpressionAtlasiQ86YS6 baseline and differential
GenevisibleiQ86YS6 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB43_HUMAN
AccessioniPrimary (citable) accession number: Q86YS6
Secondary accession number(s): A8K4P9, E9PBQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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