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Protein

Transcriptional repressor p66-alpha

Gene

GATAD2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri411 – 464GATA-typePROSITE-ProRule annotationAdd BLAST54

GO - Molecular functioni

GO - Biological processi

  • DNA methylation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: GO_Central

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214815 HDACs deacetylate histones
R-HSA-427389 ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression
R-HSA-6804758 Regulation of TP53 Activity through Acetylation
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-8943724 Regulation of PTEN gene transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor p66-alpha
Short name:
Hp66alpha
Alternative name(s):
GATA zinc finger domain-containing protein 2A
Gene namesi
Name:GATAD2A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000167491.17
HGNCiHGNC:29989 GATAD2A
MIMi614997 gene
neXtProtiNX_Q86YP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149K → R: Disruption of MBD2-binding, loss of enhancement of MBD2-mediated repression and loss of speckled nuclear localization. 1 Publication1

Organism-specific databases

DisGeNETi54815
OpenTargetsiENSG00000167491
PharmGKBiPA142671746

Polymorphism and mutation databases

BioMutaiGATAD2A
DMDMi50401012

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835001 – 633Transcriptional repressor p66-alphaAdd BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphothreonineCombined sources1
Modified residuei49PhosphothreonineCombined sources1
Cross-linki93Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei100PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei113PhosphoserineBy similarity1
Modified residuei114PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei189PhosphothreonineCombined sources1
Cross-linki204Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei225Omega-N-methylarginineBy similarity1
Cross-linki233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei249Omega-N-methylarginineCombined sources1
Modified residuei258Omega-N-methylarginineCombined sources1
Modified residuei273Omega-N-methylarginineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei285Omega-N-methylarginineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Cross-linki464Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki487Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei512PhosphoserineCombined sources1
Modified residuei539Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei539Omega-N-methylarginine; alternateCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1
Cross-linki550Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei556PhosphoserineCombined sources1
Cross-linki585Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei598PhosphoserineCombined sources1
Cross-linki605Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86YP4
MaxQBiQ86YP4
PaxDbiQ86YP4
PeptideAtlasiQ86YP4
PRIDEiQ86YP4
ProteomicsDBi70446
70447 [Q86YP4-2]

PTM databases

iPTMnetiQ86YP4
PhosphoSitePlusiQ86YP4

Miscellaneous databases

PMAP-CutDBiQ86YP4

Expressioni

Tissue specificityi

Ubiquitous, both in fetal and adult tissues.1 Publication

Gene expression databases

BgeeiENSG00000167491 Expressed in 223 organ(s), highest expression level in female gonad
CleanExiHS_GATAD2A
ExpressionAtlasiQ86YP4 baseline and differential
GenevisibleiQ86YP4 HS

Organism-specific databases

HPAiHPA006759
HPA024373

Interactioni

Subunit structurei

Binds MBD2 and MBD3. Interaction with MBD2 is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin. Component of the MeCP1 histone deacetylase complex. Interacts with histone tails, including that of histones H2A, H2B, H3 and H4. This interaction is reduced by histone acetylation.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120172, 56 interactors
ComplexPortaliCPX-880 MBD2/NuRD nucleosome remodeling and deacetylase complex
CPX-922 MBD3/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ86YP4
DIPiDIP-36053N
IntActiQ86YP4, 43 interactors
MINTiQ86YP4
STRINGi9606.ENSP00000351552

Structurei

Secondary structure

1633
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ86YP4
SMRiQ86YP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 178CR1; MBD2-bindingAdd BLAST35
Regioni340 – 480CR2; histone tail-bindingAdd BLAST141

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili139 – 174Sequence analysisAdd BLAST36

Domaini

Both CR1 and CR2 regions are required for speckled nuclear localization.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri411 – 464GATA-typePROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3740 Eukaryota
ENOG410XRVM LUCA
GeneTreeiENSGT00390000004097
HOGENOMiHOG000074070
HOVERGENiHBG053401
InParanoidiQ86YP4
OMAiPGKHAER
PhylomeDBiQ86YP4
TreeFamiTF321369

Family and domain databases

InterProiView protein in InterPro
IPR032346 P66_CC
IPR000679 Znf_GATA
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PF16563 P66_CC, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86YP4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEEACRTRS QKRALERDPT EDDVESKKIK MERGLLASDL NTDGDMRVTP
60 70 80 90 100
EPGAGPTQGL LRATEATAMA MGRGEGLVGD GPVDMRTSHS DMKSERRPPS
110 120 130 140 150
PDVIVLSDNE QPSSPRVNGL TTVALKETST EALMKSSPEE RERMIKQLKE
160 170 180 190 200
ELRLEEAKLV LLKKLRQSQI QKEATAQKPT GSVGSTVTTP PPLVRGTQNI
210 220 230 240 250
PAGKPSLQTS SARMPGSVIP PPLVRGGQQA SSKLGPQASS QVVMPPLVRG
260 270 280 290 300
AQQIHSIRQH SSTGPPPLLL APRASVPSVQ IQGQRIIQQG LIRVANVPNT
310 320 330 340 350
SLLVNIPQPT PASLKGTTAT SAQANSTPTS VASVVTSAES PASRQAAAKL
360 370 380 390 400
ALRKQLEKTL LEIPPPKPPA PEMNFLPSAA NNEFIYLVGL EEVVQNLLET
410 420 430 440 450
QGRMSAATVL SREPYMCAQC KTDFTCRWRE EKSGAIMCEN CMTTNQKKAL
460 470 480 490 500
KVEHTSRLKA AFVKALQQEQ EIEQRLLQQG TAPAQAKAEP TAAPHPVLKQ
510 520 530 540 550
VIKPRRKLAF RSGEARDWSN GAVLQASSQL SRGSATTPRG VLHTFSPSPK
560 570 580 590 600
LQNSASATAL VSRTGRHSER TVSAGKGSAT SNWKKTPLST GGTLAFVSPS
610 620 630
LAVHKSSSAV DRQREYLLDM IPPRSIPQSA TWK
Length:633
Mass (Da):68,063
Last modified:June 1, 2003 - v1
Checksum:i64F0066B4BEFCB39
GO
Isoform 2 (identifier: Q86YP4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-524: Missing.

Note: No experimental confirmation available.
Show »
Length:608
Mass (Da):65,225
Checksum:i751DB0FC42DA3BC8
GO
Isoform 3 (identifier: Q86YP4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     401-401: Q → QA

Note: No experimental confirmation available.
Show »
Length:634
Mass (Da):68,134
Checksum:i4C5D2B9CD5FE719E
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JJK9C9JJK9_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
177Annotation score:
C9JHD7C9JHD7_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
165Annotation score:
C9JMI3C9JMI3_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
126Annotation score:
C9JGN4C9JGN4_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
139Annotation score:
C9JVY3C9JVY3_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
93Annotation score:
V9GY85V9GY85_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
95Annotation score:
V9GYX5V9GYX5_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
77Annotation score:
H7C3H1H7C3H1_HUMAN
Transcriptional repressor p66-alpha
GATAD2A
99Annotation score:

Sequence cautioni

The sequence AAH11684 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA90939 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti432K → E in BAA90939 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05930817R → Q. Corresponds to variant dbSNP:rs10426883Ensembl.1
Natural variantiVAR_059309296N → S. Corresponds to variant dbSNP:rs2288851Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053410401Q → QA in isoform 3. 1 Publication1
Alternative sequenceiVSP_010929500 – 524Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186731 mRNA Translation: AAO31797.1
AC003030 Genomic DNA No translation available.
AC011448 Genomic DNA No translation available.
AC092067 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84819.1
BC011684 mRNA Translation: AAH11684.1 Different initiation.
BC012902 mRNA Translation: AAH12902.2
AK000092 mRNA Translation: BAA90939.1 Different initiation.
AL390164 mRNA Translation: CAB99095.1
CCDSiCCDS12402.2 [Q86YP4-1]
CCDS77270.1 [Q86YP4-3]
PIRiT51878
RefSeqiNP_001287875.1, NM_001300946.1 [Q86YP4-3]
NP_060130.3, NM_017660.3 [Q86YP4-1]
XP_011526407.1, XM_011528105.1 [Q86YP4-3]
UniGeneiHs.118964

Genome annotation databases

EnsembliENST00000358713; ENSP00000351552; ENSG00000167491 [Q86YP4-1]
ENST00000360315; ENSP00000353463; ENSG00000167491 [Q86YP4-1]
ENST00000404158; ENSP00000384899; ENSG00000167491 [Q86YP4-3]
GeneIDi54815
KEGGihsa:54815
UCSCiuc010xqt.3 human [Q86YP4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186731 mRNA Translation: AAO31797.1
AC003030 Genomic DNA No translation available.
AC011448 Genomic DNA No translation available.
AC092067 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84819.1
BC011684 mRNA Translation: AAH11684.1 Different initiation.
BC012902 mRNA Translation: AAH12902.2
AK000092 mRNA Translation: BAA90939.1 Different initiation.
AL390164 mRNA Translation: CAB99095.1
CCDSiCCDS12402.2 [Q86YP4-1]
CCDS77270.1 [Q86YP4-3]
PIRiT51878
RefSeqiNP_001287875.1, NM_001300946.1 [Q86YP4-3]
NP_060130.3, NM_017660.3 [Q86YP4-1]
XP_011526407.1, XM_011528105.1 [Q86YP4-3]
UniGeneiHs.118964

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L2LNMR-A137-178[»]
ProteinModelPortaliQ86YP4
SMRiQ86YP4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120172, 56 interactors
ComplexPortaliCPX-880 MBD2/NuRD nucleosome remodeling and deacetylase complex
CPX-922 MBD3/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ86YP4
DIPiDIP-36053N
IntActiQ86YP4, 43 interactors
MINTiQ86YP4
STRINGi9606.ENSP00000351552

PTM databases

iPTMnetiQ86YP4
PhosphoSitePlusiQ86YP4

Polymorphism and mutation databases

BioMutaiGATAD2A
DMDMi50401012

Proteomic databases

EPDiQ86YP4
MaxQBiQ86YP4
PaxDbiQ86YP4
PeptideAtlasiQ86YP4
PRIDEiQ86YP4
ProteomicsDBi70446
70447 [Q86YP4-2]

Protocols and materials databases

DNASUi54815
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358713; ENSP00000351552; ENSG00000167491 [Q86YP4-1]
ENST00000360315; ENSP00000353463; ENSG00000167491 [Q86YP4-1]
ENST00000404158; ENSP00000384899; ENSG00000167491 [Q86YP4-3]
GeneIDi54815
KEGGihsa:54815
UCSCiuc010xqt.3 human [Q86YP4-1]

Organism-specific databases

CTDi54815
DisGeNETi54815
EuPathDBiHostDB:ENSG00000167491.17
GeneCardsiGATAD2A
H-InvDBiHIX0014940
HGNCiHGNC:29989 GATAD2A
HPAiHPA006759
HPA024373
MIMi614997 gene
neXtProtiNX_Q86YP4
OpenTargetsiENSG00000167491
PharmGKBiPA142671746
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3740 Eukaryota
ENOG410XRVM LUCA
GeneTreeiENSGT00390000004097
HOGENOMiHOG000074070
HOVERGENiHBG053401
InParanoidiQ86YP4
OMAiPGKHAER
PhylomeDBiQ86YP4
TreeFamiTF321369

Enzyme and pathway databases

ReactomeiR-HSA-3214815 HDACs deacetylate histones
R-HSA-427389 ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression
R-HSA-6804758 Regulation of TP53 Activity through Acetylation
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-8943724 Regulation of PTEN gene transcription

Miscellaneous databases

ChiTaRSiGATAD2A human
GenomeRNAii54815
PMAP-CutDBiQ86YP4
PROiPR:Q86YP4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167491 Expressed in 223 organ(s), highest expression level in female gonad
CleanExiHS_GATAD2A
ExpressionAtlasiQ86YP4 baseline and differential
GenevisibleiQ86YP4 HS

Family and domain databases

InterProiView protein in InterPro
IPR032346 P66_CC
IPR000679 Znf_GATA
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PF16563 P66_CC, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP66A_HUMAN
AccessioniPrimary (citable) accession number: Q86YP4
Secondary accession number(s): B5MC40
, Q7L3J2, Q96F28, Q9NPU2, Q9NXS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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