UniProtKB - Q86YJ6 (THNS2_HUMAN)
Protein
Threonine synthase-like 2
Gene
THNSL2
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate (By similarity).By similarity
Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions.1 Publication
Cofactori
pyridoxal 5'-phosphateBy similarity
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- lyase activity Source: UniProtKB-KW
- pyridoxal phosphate binding Source: BHF-UCL
- serine binding Source: BHF-UCL
GO - Biological processi
- 2-oxobutyrate biosynthetic process Source: BHF-UCL
- dephosphorylation Source: BHF-UCL
- serine family amino acid catabolic process Source: BHF-UCL
Keywordsi
Molecular function | Cytokine, Lyase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
PathwayCommonsi | Q86YJ6 |
SignaLinki | Q86YJ6 |
Names & Taxonomyi
Protein namesi | Recommended name: Threonine synthase-like 2 (EC:4.2.3.-)Short name: TSH2 Alternative name(s): Secreted osteoclastogenic factor of activated T-cells Short name: SOFAT |
Gene namesi | Name:THNSL2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25602, THNSL2 |
MIMi | 611261, gene |
neXtProti | NX_Q86YJ6 |
VEuPathDBi | HostDB:ENSG00000144115.16 |
Subcellular locationi
Extracellular region or secreted
Note: Secreted by activated T-cells via a calcineurin-independent pathway.
Extracellular region or secreted
- extracellular space Source: UniProtKB-KW
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 55258 |
OpenTargetsi | ENSG00000144115 |
PharmGKBi | PA162405692 |
Miscellaneous databases
Pharosi | Q86YJ6, Tbio |
Genetic variation databases
BioMutai | THNSL2 |
DMDMi | 313104279 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306407 | 1 – 484 | Threonine synthase-like 2Add BLAST | 484 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 113 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
EPDi | Q86YJ6 |
MassIVEi | Q86YJ6 |
MaxQBi | Q86YJ6 |
PaxDbi | Q86YJ6 |
PeptideAtlasi | Q86YJ6 |
PRIDEi | Q86YJ6 |
ProteomicsDBi | 70421 [Q86YJ6-1] 70422 [Q86YJ6-2] 70423 [Q86YJ6-3] 70424 [Q86YJ6-4] 70425 [Q86YJ6-5] |
PTM databases
PhosphoSitePlusi | Q86YJ6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000144115, Expressed in adenohypophysis and 204 other tissues |
ExpressionAtlasi | Q86YJ6, baseline and differential |
Genevisiblei | Q86YJ6, HS |
Organism-specific databases
HPAi | ENSG00000144115, Low tissue specificity |
Interactioni
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 120549, 1 interactor |
IntActi | Q86YJ6, 3 interactors |
STRINGi | 9606.ENSP00000327323 |
Miscellaneous databases
RNActi | Q86YJ6, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the threonine synthase family.Curated
Phylogenomic databases
eggNOGi | KOG2616, Eukaryota |
GeneTreei | ENSGT00940000158503 |
HOGENOMi | CLU_015170_1_1_1 |
InParanoidi | Q86YJ6 |
OMAi | TYYFYSY |
OrthoDBi | 1361511at2759 |
PhylomeDBi | Q86YJ6 |
TreeFami | TF329641 |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits 3.90.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR029144, Thr_synth_N IPR037158, Thr_synth_N_sf IPR004450, Thr_synthase-like IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit PF14821, Thr_synth_N, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR00260, thrC, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q86YJ6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS
60 70 80 90 100
YPGLVKELCA LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN
110 120 130 140 150
VLELWHGVTY AFKDLSLSCT TQFLQYFLEK REKHVTVVVG TSGDTGSAAI
160 170 180 190 200
ESVQGAKNMD IIVLLPKGHC TKIQELQMTT VLKQNVHVFG VEGNSDELDE
210 220 230 240 250
PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF QCTPSLDTHP
260 270 280 290 300
LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF
310 320 330 340 350
SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV
360 370 380 390 400
NLPKELHSKL SEAVTSVSVS DEAITQTMGR CWDENQYLLC PHSAVAVNYH
410 420 430 440 450
YQQIDRQQPS TPRCCLAPAS AAKFPEAVLA AGLTPETPAE IVALEHKETR
460 470 480
CTLMRRGDNW MLMLRDTIED LSRQWRSHAL NTSQ
Isoform SOFAT (identifier: Q86YJ6-5) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-158: Missing.
360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGD
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8K0C1 | A8K0C1_HUMAN | Threonine synthase-like 2 | THNSL2 | 226 | Annotation score: | ||
C9JU10 | C9JU10_HUMAN | Threonine synthase-like 2 | THNSL2 | 258 | Annotation score: | ||
C9J3J1 | C9J3J1_HUMAN | Threonine synthase-like 2 | THNSL2 | 126 | Annotation score: |
Sequence cautioni
The sequence AAX88906 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA91904 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | I → V in BAA91904 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_054635 | 41 | G → E2 PublicationsCorresponds to variant dbSNP:rs4129190Ensembl. | 1 | |
Natural variantiVAR_054636 | 108 | V → I. Corresponds to variant dbSNP:rs35541720Ensembl. | 1 | |
Natural variantiVAR_054637 | 204 | T → N. Corresponds to variant dbSNP:rs34136143Ensembl. | 1 | |
Natural variantiVAR_054638 | 324 | R → G1 PublicationCorresponds to variant dbSNP:rs17855905Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041659 | 1 – 158 | Missing in isoform SOFAT. 1 PublicationAdd BLAST | 158 | |
Alternative sequenceiVSP_028468 | 214 – 274 | Missing in isoform 4. 1 PublicationAdd BLAST | 61 | |
Alternative sequenceiVSP_028469 | 360 – 484 | LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGG in isoform 2. 1 PublicationAdd BLAST | 125 | |
Alternative sequenceiVSP_028470 | 360 – 484 | LSEAV…LNTSQ → HSPVLPRPCLCSQVPGSCPG CWPDP in isoform 3. 1 PublicationAdd BLAST | 125 | |
Alternative sequenceiVSP_041660 | 360 – 484 | LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGD in isoform SOFAT. 1 PublicationAdd BLAST | 125 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM185274 mRNA Translation: ADL14696.1 AK001778 mRNA Translation: BAA91904.1 Different initiation. AK095303 mRNA Translation: BAG53023.1 AC092836 Genomic DNA Translation: AAX88906.1 Sequence problems. BC035315 mRNA Translation: AAH35315.1 BC047758 mRNA Translation: AAH47758.2 BC064423 mRNA Translation: AAH64423.1 |
CCDSi | CCDS2002.2 [Q86YJ6-1] CCDS58718.1 [Q86YJ6-2] |
RefSeqi | NP_001231605.1, NM_001244676.1 [Q86YJ6-2] NP_060741.3, NM_018271.4 [Q86YJ6-1] XP_005264457.1, XM_005264400.4 XP_005264458.1, XM_005264401.4 [Q86YJ6-1] XP_005264460.1, XM_005264403.4 XP_006712106.1, XM_006712043.2 [Q86YJ6-2] |
Genome annotation databases
Ensembli | ENST00000324166; ENSP00000327323; ENSG00000144115 [Q86YJ6-1] ENST00000343544; ENSP00000339563; ENSG00000144115 [Q86YJ6-2] ENST00000496844; ENSP00000501447; ENSG00000144115 [Q86YJ6-3] ENST00000674334; ENSP00000501453; ENSG00000144115 [Q86YJ6-1] |
GeneIDi | 55258 |
KEGGi | hsa:55258 |
UCSCi | uc002ssw.5, human [Q86YJ6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM185274 mRNA Translation: ADL14696.1 AK001778 mRNA Translation: BAA91904.1 Different initiation. AK095303 mRNA Translation: BAG53023.1 AC092836 Genomic DNA Translation: AAX88906.1 Sequence problems. BC035315 mRNA Translation: AAH35315.1 BC047758 mRNA Translation: AAH47758.2 BC064423 mRNA Translation: AAH64423.1 |
CCDSi | CCDS2002.2 [Q86YJ6-1] CCDS58718.1 [Q86YJ6-2] |
RefSeqi | NP_001231605.1, NM_001244676.1 [Q86YJ6-2] NP_060741.3, NM_018271.4 [Q86YJ6-1] XP_005264457.1, XM_005264400.4 XP_005264458.1, XM_005264401.4 [Q86YJ6-1] XP_005264460.1, XM_005264403.4 XP_006712106.1, XM_006712043.2 [Q86YJ6-2] |
3D structure databases
SMRi | Q86YJ6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120549, 1 interactor |
IntActi | Q86YJ6, 3 interactors |
STRINGi | 9606.ENSP00000327323 |
PTM databases
PhosphoSitePlusi | Q86YJ6 |
Genetic variation databases
BioMutai | THNSL2 |
DMDMi | 313104279 |
Proteomic databases
EPDi | Q86YJ6 |
MassIVEi | Q86YJ6 |
MaxQBi | Q86YJ6 |
PaxDbi | Q86YJ6 |
PeptideAtlasi | Q86YJ6 |
PRIDEi | Q86YJ6 |
ProteomicsDBi | 70421 [Q86YJ6-1] 70422 [Q86YJ6-2] 70423 [Q86YJ6-3] 70424 [Q86YJ6-4] 70425 [Q86YJ6-5] |
Protocols and materials databases
Antibodypediai | 32227, 128 antibodies |
Genome annotation databases
Ensembli | ENST00000324166; ENSP00000327323; ENSG00000144115 [Q86YJ6-1] ENST00000343544; ENSP00000339563; ENSG00000144115 [Q86YJ6-2] ENST00000496844; ENSP00000501447; ENSG00000144115 [Q86YJ6-3] ENST00000674334; ENSP00000501453; ENSG00000144115 [Q86YJ6-1] |
GeneIDi | 55258 |
KEGGi | hsa:55258 |
UCSCi | uc002ssw.5, human [Q86YJ6-1] |
Organism-specific databases
CTDi | 55258 |
DisGeNETi | 55258 |
GeneCardsi | THNSL2 |
HGNCi | HGNC:25602, THNSL2 |
HPAi | ENSG00000144115, Low tissue specificity |
MIMi | 611261, gene |
neXtProti | NX_Q86YJ6 |
OpenTargetsi | ENSG00000144115 |
PharmGKBi | PA162405692 |
VEuPathDBi | HostDB:ENSG00000144115.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2616, Eukaryota |
GeneTreei | ENSGT00940000158503 |
HOGENOMi | CLU_015170_1_1_1 |
InParanoidi | Q86YJ6 |
OMAi | TYYFYSY |
OrthoDBi | 1361511at2759 |
PhylomeDBi | Q86YJ6 |
TreeFami | TF329641 |
Enzyme and pathway databases
PathwayCommonsi | Q86YJ6 |
SignaLinki | Q86YJ6 |
Miscellaneous databases
BioGRID-ORCSi | 55258, 3 hits in 871 CRISPR screens |
ChiTaRSi | THNSL2, human |
GenomeRNAii | 55258 |
Pharosi | Q86YJ6, Tbio |
PROi | PR:Q86YJ6 |
RNActi | Q86YJ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000144115, Expressed in adenohypophysis and 204 other tissues |
ExpressionAtlasi | Q86YJ6, baseline and differential |
Genevisiblei | Q86YJ6, HS |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits 3.90.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR029144, Thr_synth_N IPR037158, Thr_synth_N_sf IPR004450, Thr_synthase-like IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit PF14821, Thr_synth_N, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR00260, thrC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THNS2_HUMAN | |
Accessioni | Q86YJ6Primary (citable) accession number: Q86YJ6 Secondary accession number(s): B3KTB1 Q9NV54 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM