UniProtKB - Q86YI5 (Q86YI5_HUMAN)
Protein
Acetyltransferase component of pyruvate dehydrogenase complex
Gene
DLAT
Organism
Homo sapiens (Human)
Status
Functioni
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.UniRule annotation
Catalytic activityi
- (R)-N6-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-N6-(S8-acetyldihydrolipoyl)-L-lysyl-[protein] + CoAUniRule annotationARBA annotationEC:2.3.1.12UniRule annotationARBA annotation
Cofactori
(R)-lipoateUniRule annotationNote: Binds 2 lipoyl cofactors covalently.UniRule annotation
GO - Molecular functioni
- dihydrolipoyllysine-residue acetyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- acetyl-CoA biosynthetic process from pyruvate Source: Ensembl
- sleep Source: Ensembl
Keywordsi
Molecular function | AcyltransferaseUniRule annotation, Transferase |
Ligand | PyruvateImported |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyltransferase component of pyruvate dehydrogenase complexUniRule annotation (EC:2.3.1.12UniRule annotation) |
Gene namesi | Name:DLATImported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Organism-specific databases
EuPathDBi | HostDB:ENSG00000150768.15 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix UniRule annotation
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: HPA
Other locations
- pyruvate dehydrogenase complex Source: Ensembl
Expressioni
Gene expression databases
Bgeei | ENSG00000150768, Expressed in heart and 246 other tissues |
ExpressionAtlasi | Q86YI5, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 167 | Lipoyl-bindingInterPro annotationAdd BLAST | 77 | |
Domaini | 218 – 294 | Lipoyl-bindingInterPro annotationAdd BLAST | 77 | |
Domaini | 356 – 393 | Peripheral subunit-binding (PSBD)InterPro annotationAdd BLAST | 38 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 29 – 49 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 184 – 219 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 312 – 346 | DisorderedSequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 190 – 204 | Pro-richSequence analysisAdd BLAST | 15 | |
Compositional biasi | 314 – 346 | Pro-richSequence analysisAdd BLAST | 33 |
Sequence similaritiesi
Keywords - Domaini
LipoylUniRule annotationARBA annotation, Transit peptideARBA annotationPhylogenomic databases
OMAi | TMEFESF |
OrthoDBi | 747232at2759 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR036625, E3-bd_dom_sf IPR006257, LAT1 IPR004167, PSBD IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 2 hits PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 2 hits |
TIGRFAMsi | TIGR01349, PDHac_trf_mito, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 2 hits PS00189, LIPOYL, 2 hits PS51826, PSBD, 1 hit |
i Sequence
Sequence statusi: Complete.
Q86YI5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT
60 70 80 90 100
GYGGVRALCG WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS
110 120 130 140 150
PTMQAGTIAR WEKKEGDKIN EGDLIAEVET DKATVGFESL EECYMAKILV
160 170 180 190 200
AEGTRDVPIG AIICITVGKP EDIEAFKNYT LDSSAAPTPQ AAPAPTPAAT
210 220 230 240 250
ASPPTPSAQA PGSSYPPHMQ VLLPALSPTM TMGTVQRWEK KVGEKLSEGD
260 270 280 290 300
LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKEADI
310 320 330 340 350
SAFADYRPTE VTDLKPQVPP PTPPPVAAVP PTPQPLAPTP SAPCPATPAG
360 370 380 390 400
PKGRVFVSPL AKKLAVEKGI DLTQVKGTGP DGRITKKDID SFVPSKVAPA
410 420 430 440 450
PAAVVPPTGP GMAPVPTGVF TDIPISNIRR VIAQRLMQSK QTIPHYYLSI
460 470 480 490 500
DVNMGEVLLV RKELNKILEG RSKISVNDFI IKASALACLK VPEANSSWMD
510 520 530 540 550
TVIRQNHVVD VSVAVSTPAG LITPIVFNAH IKGVETIAND VVSLATKARE
560 570 580 590 600
GKLQPHEFQG GTFTISNLGM FGIKNFSAII NPPQACILAI GASEDKLVPA
610 620 630 640
DNEKGFDVAS MMSVTLSCDH RVVDGAVGAQ WLAEFRKYLE KPITMLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC039084 mRNA Translation: AAH39084.1 EF444972 Genomic DNA Translation: ACA05975.1 AK057299 mRNA Translation: BAG51900.1 |
RefSeqi | NP_001922.2, NM_001931.4 |
Genome annotation databases
GeneIDi | 1737 |
KEGGi | hsa:1737 |
UCSCi | uc001pmo.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC039084 mRNA Translation: AAH39084.1 EF444972 Genomic DNA Translation: ACA05975.1 AK057299 mRNA Translation: BAG51900.1 |
RefSeqi | NP_001922.2, NM_001931.4 |
3D structure databases
SMRi | Q86YI5 |
ModBasei | Search... |
Proteomic databases
MaxQBi | Q86YI5 |
PRIDEi | Q86YI5 |
Protocols and materials databases
Antibodypediai | 18282, 299 antibodies |
DNASUi | 1737 |
Genome annotation databases
GeneIDi | 1737 |
KEGGi | hsa:1737 |
UCSCi | uc001pmo.4, human |
Organism-specific databases
CTDi | 1737 |
EuPathDBi | HostDB:ENSG00000150768.15 |
PharmGKBi | PA27350 |
Phylogenomic databases
OMAi | TMEFESF |
OrthoDBi | 747232at2759 |
Miscellaneous databases
BioGRID-ORCSi | 1737, 16 hits in 845 CRISPR screens |
ChiTaRSi | DLAT, human |
GenomeRNAii | 1737 |
Gene expression databases
Bgeei | ENSG00000150768, Expressed in heart and 246 other tissues |
ExpressionAtlasi | Q86YI5, baseline and differential |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR036625, E3-bd_dom_sf IPR006257, LAT1 IPR004167, PSBD IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 2 hits PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 2 hits |
TIGRFAMsi | TIGR01349, PDHac_trf_mito, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 2 hits PS00189, LIPOYL, 2 hits PS51826, PSBD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q86YI5_HUMAN | |
Accessioni | Q86YI5Primary (citable) accession number: Q86YI5 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | June 1, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |