UniProtKB - Q86YB8 (ERO1B_HUMAN)
ERO1-like protein beta
ERO1B
Functioni
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.
2 PublicationsCofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 186 | FADBy similarity | 1 | |
Binding sitei | 188 | FADBy similarity | 1 | |
Binding sitei | 199 | FADBy similarity | 1 | |
Binding sitei | 251 | FADBy similarity | 1 | |
Binding sitei | 254 | FADBy similarity | 1 | |
Binding sitei | 286 | FADBy similarity | 1 | |
Binding sitei | 299 | FADBy similarity | 1 |
GO - Molecular functioni
- FAD binding Source: InterPro
- oxidoreductase activity Source: UniProtKB
- protein-disulfide reductase activity Source: GO_Central
- thiol oxidase activity Source: FlyBase
- unfolded protein binding Source: UniProtKB
GO - Biological processi
- protein folding Source: UniProtKB
- protein folding in endoplasmic reticulum Source: FlyBase
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
PathwayCommonsi | Q86YB8 |
Reactomei | R-HSA-264876, Insulin processing |
SignaLinki | Q86YB8 |
SIGNORi | Q86YB8 |
Names & Taxonomyi
Protein namesi | Recommended name: ERO1-like protein beta (EC:1.8.4.-)Short name: ERO1-L-beta Alternative name(s): Endoplasmic reticulum oxidoreductase betaImported Endoplasmic reticulum oxidoreductin-1-like protein B Oxidoreductin-1-L-beta |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14355, ERO1B |
MIMi | 615437, gene |
neXtProti | NX_Q86YB8 |
VEuPathDBi | HostDB:ENSG00000086619 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication; Lumenal side 1 Publication
Note: The association with ERP44 may be essential for its retention in the endoplasmic reticulum.
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | C → A: No effect on catalytic activity when DTT is used as an electron donor; when associated with A-95. Loss of catalytic activity when P4HB is used as an electron donor; when associated with A-95. 1 Publication | 1 | |
Mutagenesisi | 95 | C → A: No effect on catalytic activity when DTT is used as an electron donor; when associated with A-90. Loss of catalytic activity when P4HB is used as an electron donor; when associated with A-90. 1 Publication | 1 | |
Mutagenesisi | 100 | C → A: Slightly decrease in activity. Significant increased activity; when associated with A-130. 1 Publication | 1 | |
Mutagenesisi | 130 | C → A: Small increase in activity. Significant increased activity; when associated with A-130. 1 Publication | 1 | |
Mutagenesisi | 390 | C → A: Strong decrease in P4HB-binding. Efficient homodimerization with both wild-type and A-390 mutated protein. 1 Publication | 1 | |
Mutagenesisi | 393 | C → A: Some decrease in P4HB-binding. Efficient homodimerization with wild-type protein. Loss of catalytic activity when DTT or P4HB are used as an electron donor; when associated with A-396. 2 Publications | 1 | |
Mutagenesisi | 396 | C → A: Some decrease in P4HB-binding. Efficient homodimerization with wild-type protein, but loss of homodimerization with A-396 mutated protein. Loss of catalytic activity when DTT or P4HB are used as an electron donor; when associated with A-393. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 56605 |
OpenTargetsi | ENSG00000086619 |
PharmGKBi | PA134918597 |
Miscellaneous databases
Pharosi | Q86YB8, Tbio |
Chemistry databases
DrugBanki | DB01902, 1-Ethyl-Pyrrolidine-2,5-Dione DB03147, Flavin adenine dinucleotide |
Genetic variation databases
BioMutai | ERO1B |
DMDMi | 116241353 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Sequence analysisAdd BLAST | 33 | |
ChainiPRO_0000008418 | 34 – 467 | ERO1-like protein betaAdd BLAST | 434 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 81 ↔ 390 | 1 Publication | ||
Disulfide bondi | 90 ↔ 130 | Alternate1 Publication | ||
Disulfide bondi | 90 ↔ 95 | Redox-active; alternate1 Publication | ||
Disulfide bondi | 100 ↔ 262 | 1 Publication | ||
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 140 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 145 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 207 ↔ 240 | 1 Publication | ||
Glycosylationi | 383 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 393 ↔ 396 | Redox-active1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q86YB8 |
jPOSTi | Q86YB8 |
MassIVEi | Q86YB8 |
MaxQBi | Q86YB8 |
PaxDbi | Q86YB8 |
PeptideAtlasi | Q86YB8 |
PRIDEi | Q86YB8 |
ProteomicsDBi | 4009 70394 [Q86YB8-1] |
PTM databases
GlyConnecti | 1226, 3 N-Linked glycans (1 site) |
GlyGeni | Q86YB8, 4 sites, 3 N-linked glycans (1 site) |
iPTMneti | Q86YB8 |
PhosphoSitePlusi | Q86YB8 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000086619, Expressed in body of pancreas and 203 other tissues |
ExpressionAtlasi | Q86YB8, baseline and differential |
Genevisiblei | Q86YB8, HS |
Organism-specific databases
HPAi | ENSG00000086619, Tissue enhanced (pancreas) |
Interactioni
Subunit structurei
Homodimer; disulfide-linked. Heterodimer with ERO1A; disulfide-linked. Also detected as monomer. Homodimers may be somewhat less active than monomers.
Interacts with P4HB.
Interacts with ERP44.
3 PublicationsBinary interactionsi
Q86YB8
With | #Exp. | IntAct |
---|---|---|
PDIA3 [P30101] | 2 | EBI-2806988,EBI-979862 |
GO - Molecular functioni
- unfolded protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 121155, 51 interactors |
IntActi | Q86YB8, 21 interactors |
MINTi | Q86YB8 |
STRINGi | 9606.ENSP00000346635 |
Miscellaneous databases
RNActi | Q86YB8, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Redox-active center, SignalPhylogenomic databases
eggNOGi | KOG2608, Eukaryota |
GeneTreei | ENSGT00390000007753 |
HOGENOMi | CLU_023061_2_2_1 |
InParanoidi | Q86YB8 |
OMAi | RDYCVPD |
OrthoDBi | 1181226at2759 |
PhylomeDBi | Q86YB8 |
TreeFami | TF314471 |
Family and domain databases
InterProi | View protein in InterPro IPR007266, Ero1 IPR037192, ERO1-like_sf |
PANTHERi | PTHR12613, PTHR12613, 1 hit |
Pfami | View protein in Pfam PF04137, ERO1, 1 hit |
PIRSFi | PIRSF017205, ERO1, 1 hit |
SUPFAMi | SSF110019, SSF110019, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSQGVRRAGA GQGVAAAVQL LVTLSFLRSV VEAQVTGVLD DCLCDIDSID
60 70 80 90 100
NFNTYKIFPK IKKLQERDYF RYYKVNLKRP CPFWAEDGHC SIKDCHVEPC
110 120 130 140 150
PESKIPVGIK AGHSNKYLKM ANNTKELEDC EQANKLGAIN STLSNQSKEA
160 170 180 190 200
FIDWARYDDS RDHFCELDDE RSPAAQYVDL LLNPERYTGY KGTSAWRVWN
210 220 230 240 250
SIYEENCFKP RSVYRPLNPL APSRGEDDGE SFYTWLEGLC LEKRVFYKLI
260 270 280 290 300
SGLHASINLH LCANYLLEET WGKPSWGPNI KEFKHRFDPV ETKGEGPRRL
310 320 330 340 350
KNLYFLYLIE LRALSKVAPY FERSIVDLYT GNAEEDADTK TLLLNIFQDT
360 370 380 390 400
KSFPMHFDEK SMFAGDKKGA KSLKEEFRLH FKNISRIMDC VGCDKCRLWG
410 420 430 440 450
KLQTQGLGTA LKILFSEKEI QKLPENSPSK GFQLTRQEIV ALLNAFGRLS
460
TSIRDLQNFK VLLQHSR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5KWQ1 | A0A8I5KWQ1_HUMAN | ERO1-like protein beta | ERO1B | 348 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028012 | 129 | D → V1 PublicationCorresponds to variant dbSNP:rs2477599Ensembl. | 1 | |
Natural variantiVAR_019492 | 465 | H → Q1 PublicationCorresponds to variant dbSNP:rs1055851Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056988 | 144 – 466 | Missing in isoform 2. 1 PublicationAdd BLAST | 323 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF252538 mRNA Translation: AAF97547.1 AK293941 mRNA Translation: BAG57318.1 AL139162 Genomic DNA No translation available. AL450309 Genomic DNA No translation available. BC032823 mRNA Translation: AAH32823.2 BC044573 mRNA Translation: AAH44573.1 |
CCDSi | CCDS31064.1 [Q86YB8-1] |
RefSeqi | NP_063944.3, NM_019891.3 [Q86YB8-1] |
Genome annotation databases
Ensembli | ENST00000354619.10; ENSP00000346635.5; ENSG00000086619.15 |
GeneIDi | 56605 |
KEGGi | hsa:56605 |
MANE-Selecti | ENST00000354619.10; ENSP00000346635.5; NM_019891.4; NP_063944.3 |
UCSCi | uc001hxt.4, human [Q86YB8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF252538 mRNA Translation: AAF97547.1 AK293941 mRNA Translation: BAG57318.1 AL139162 Genomic DNA No translation available. AL450309 Genomic DNA No translation available. BC032823 mRNA Translation: AAH32823.2 BC044573 mRNA Translation: AAH44573.1 |
CCDSi | CCDS31064.1 [Q86YB8-1] |
RefSeqi | NP_063944.3, NM_019891.3 [Q86YB8-1] |
3D structure databases
AlphaFoldDBi | Q86YB8 |
SMRi | Q86YB8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121155, 51 interactors |
IntActi | Q86YB8, 21 interactors |
MINTi | Q86YB8 |
STRINGi | 9606.ENSP00000346635 |
Chemistry databases
DrugBanki | DB01902, 1-Ethyl-Pyrrolidine-2,5-Dione DB03147, Flavin adenine dinucleotide |
PTM databases
GlyConnecti | 1226, 3 N-Linked glycans (1 site) |
GlyGeni | Q86YB8, 4 sites, 3 N-linked glycans (1 site) |
iPTMneti | Q86YB8 |
PhosphoSitePlusi | Q86YB8 |
Genetic variation databases
BioMutai | ERO1B |
DMDMi | 116241353 |
Proteomic databases
EPDi | Q86YB8 |
jPOSTi | Q86YB8 |
MassIVEi | Q86YB8 |
MaxQBi | Q86YB8 |
PaxDbi | Q86YB8 |
PeptideAtlasi | Q86YB8 |
PRIDEi | Q86YB8 |
ProteomicsDBi | 4009 70394 [Q86YB8-1] |
Protocols and materials databases
Antibodypediai | 34698, 199 antibodies from 25 providers |
DNASUi | 56605 |
Genome annotation databases
Ensembli | ENST00000354619.10; ENSP00000346635.5; ENSG00000086619.15 |
GeneIDi | 56605 |
KEGGi | hsa:56605 |
MANE-Selecti | ENST00000354619.10; ENSP00000346635.5; NM_019891.4; NP_063944.3 |
UCSCi | uc001hxt.4, human [Q86YB8-1] |
Organism-specific databases
CTDi | 56605 |
DisGeNETi | 56605 |
GeneCardsi | ERO1B |
HGNCi | HGNC:14355, ERO1B |
HPAi | ENSG00000086619, Tissue enhanced (pancreas) |
MIMi | 615437, gene |
neXtProti | NX_Q86YB8 |
OpenTargetsi | ENSG00000086619 |
PharmGKBi | PA134918597 |
VEuPathDBi | HostDB:ENSG00000086619 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2608, Eukaryota |
GeneTreei | ENSGT00390000007753 |
HOGENOMi | CLU_023061_2_2_1 |
InParanoidi | Q86YB8 |
OMAi | RDYCVPD |
OrthoDBi | 1181226at2759 |
PhylomeDBi | Q86YB8 |
TreeFami | TF314471 |
Enzyme and pathway databases
PathwayCommonsi | Q86YB8 |
Reactomei | R-HSA-264876, Insulin processing |
SignaLinki | Q86YB8 |
SIGNORi | Q86YB8 |
Miscellaneous databases
BioGRID-ORCSi | 56605, 9 hits in 1073 CRISPR screens |
ChiTaRSi | ERO1B, human |
GeneWikii | ERO1LB |
GenomeRNAii | 56605 |
Pharosi | Q86YB8, Tbio |
PROi | PR:Q86YB8 |
RNActi | Q86YB8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000086619, Expressed in body of pancreas and 203 other tissues |
ExpressionAtlasi | Q86YB8, baseline and differential |
Genevisiblei | Q86YB8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007266, Ero1 IPR037192, ERO1-like_sf |
PANTHERi | PTHR12613, PTHR12613, 1 hit |
Pfami | View protein in Pfam PF04137, ERO1, 1 hit |
PIRSFi | PIRSF017205, ERO1, 1 hit |
SUPFAMi | SSF110019, SSF110019, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERO1B_HUMAN | |
Accessioni | Q86YB8Primary (citable) accession number: Q86YB8 Secondary accession number(s): B4DF57 Q9NR62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | October 17, 2006 | |
Last modified: | May 25, 2022 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families