UniProtKB - Q86Y97 (KMT5C_HUMAN)
Histone-lysine N-methyltransferase KMT5C
KMT5C
Functioni
Catalytic activityi
- N6-methyl-L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6,N6-dimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.3622 PublicationsThis reaction proceeds in the forward1 Publication direction.
- N6,N6-dimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6,N6,N6-trimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6-methyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.3611 Publication
Activity regulationi
Kineticsi
- KM=21 µM for H4K20me01 Publication
- KM=16 µM for H4K20me11 Publication
- KM=0.5 µM for nucleosome1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 121 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 160 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 169 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Metal bindingi | 185 | ZincCombined sources1 Publication | 1 | |
Metal bindingi | 229 | ZincCombined sources1 Publication | 1 | |
Binding sitei | 230 | S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 231 | ZincCombined sources1 Publication | 1 | |
Metal bindingi | 234 | ZincCombined sources1 Publication | 1 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- histone binding Source: Ensembl
- histone methyltransferase activity (H4-K20 specific) Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- S-adenosyl-L-methionine binding Source: UniProtKB
GO - Biological processi
- DNA repair Source: UniProtKB
- histone H4-K20 trimethylation Source: GO_Central
- positive regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
- positive regulation of isotype switching Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:HS13469-MONOMER |
PathwayCommonsi | Q86Y97 |
Reactomei | R-HSA-3214841, PKMTs methylate histone lysines |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase KMT5CCuratedAlternative name(s): Lysine N-methyltransferase 5C Lysine-specific methyltransferase 5CImported Suppressor of variegation 4-20 homolog 2 Short name: Su(var)4-20 homolog 2 Short name: Suv4-20h2 [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5BCurated (EC:2.1.1.3622 Publications) [histone H4]-lysine20 N-methyltransferase KMT5BCurated (EC:2.1.1.3611 Publication) |
Gene namesi | ORF Names:PP7130 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28405, KMT5C |
MIMi | 613198, gene |
neXtProti | NX_Q86Y97 |
VEuPathDBi | HostDB:ENSG00000133247.13 |
Subcellular locationi
Nucleus
Other locations
- Chromosome By similarity
Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).By similarity
Nucleus
- condensed nuclear chromosome, centromeric region Source: Ensembl
- nuclear heterochromatin Source: BHF-UCL
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- pericentric heterochromatin Source: BHF-UCL
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 84787 |
OpenTargetsi | ENSG00000133247 |
PharmGKBi | PA134934307 |
Miscellaneous databases
Pharosi | Q86Y97, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2321644 |
GuidetoPHARMACOLOGYi | 2718 |
Genetic variation databases
BioMutai | KMT5C |
DMDMi | 74727906 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281793 | 1 – 462 | Histone-lysine N-methyltransferase KMT5CAdd BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 416 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 422 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q86Y97 |
jPOSTi | Q86Y97 |
MassIVEi | Q86Y97 |
MaxQBi | Q86Y97 |
PaxDbi | Q86Y97 |
PeptideAtlasi | Q86Y97 |
PRIDEi | Q86Y97 |
ProteomicsDBi | 70387 [Q86Y97-1] 70388 [Q86Y97-2] |
PTM databases
iPTMneti | Q86Y97 |
PhosphoSitePlusi | Q86Y97 |
Expressioni
Gene expression databases
Bgeei | ENSG00000133247, Expressed in layer of synovial tissue and 220 other tissues |
ExpressionAtlasi | Q86Y97, baseline and differential |
Genevisiblei | Q86Y97, HS |
Organism-specific databases
HPAi | ENSG00000133247, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with HP1 proteins CBX1, CBX3 and CBX5.
Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity).
By similarityBinary interactionsi
Q86Y97
With | #Exp. | IntAct |
---|---|---|
CBX3 [Q13185] | 3 | EBI-7960569,EBI-78176 |
GO - Molecular functioni
- histone binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 124257, 12 interactors |
IntActi | Q86Y97, 29 interactors |
MINTi | Q86Y97 |
STRINGi | 9606.ENSP00000255613 |
Chemistry databases
BindingDBi | Q86Y97 |
Miscellaneous databases
RNActi | Q86Y97, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q86Y97 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 218 | SETPROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 114 – 117 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 4 | |
Regioni | 182 – 183 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 | |
Regioni | 346 – 435 | Required for heterochromatin localizationBy similarityAdd BLAST | 90 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2589, Eukaryota |
GeneTreei | ENSGT00940000161700 |
HOGENOMi | CLU_040002_0_0_1 |
InParanoidi | Q86Y97 |
OMAi | LHRWGGC |
OrthoDBi | 236983at2759 |
PhylomeDBi | Q86Y97 |
TreeFami | TF106433 |
Family and domain databases
Gene3Di | 1.10.10.1700, 1 hit |
InterProi | View protein in InterPro IPR041938, Hist-Lys_N-MTase_N IPR025790, Hist-Lys_N-MTase_Suvar4-20 IPR039977, KMT5B/KMT5C/SET9 IPR001214, SET_dom |
PANTHERi | PTHR12977, PTHR12977, 1 hit |
Pfami | View protein in Pfam PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51570, SAM_MT43_SUVAR420_2, 1 hit PS50280, SET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGPDRVTARE LCENDDLATS LVLDPYLGFR THKMNVSPVP PLRRQQHLRS
60 70 80 90 100
ALETFLRQRD LEAAYRALTL GGWTARYFQS RGPRQEAALK THVYRYLRAF
110 120 130 140 150
LPESGFTILP CTRYSMETNG AKIVSTRAWK KNEKLELLVG CIAELREADE
160 170 180 190 200
GLLRAGENDF SIMYSTRKRS AQLWLGPAAF INHDCKPNCK FVPADGNAAC
210 220 230 240 250
VKVLRDIEPG DEVTCFYGEG FFGEKNEHCE CHTCERKGEG AFRTRPREPA
260 270 280 290 300
LPPRPLDKYQ LRETKRRLQQ GLDSGSRQGL LGPRACVHPS PLRRDPFCAA
310 320 330 340 350
CQPLRLPACS ARPDTSPLWL QWLPQPQPRV RPRKRRRPRP RRAPVLSTHH
360 370 380 390 400
AARVSLHRWG GCGPHCRLRG EALVALGQPP HARWAPQQDW HWARRYGLPY
410 420 430 440 450
VVRVDLRRLA PAPPATPAPA GTPGPILIPK QALAFAPFSP PKRLRLVVSH
460
GSIDLDVGGE EL
The sequence of this isoform differs from the canonical sequence as follows:
37-97: SPVPPLRRQQ...ALKTHVYRYL → RSIATSVPSC...QSCGRQMRGC
98-462: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0D9SF94 | A0A0D9SF94_HUMAN | [histone H4]-N-methyl-L-lysine20 N-... | KMT5C SUV420H2, hCG_38236 | 347 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043947 | 37 – 97 | SPVPP…VYRYL → RSIATSVPSCRKVALPSCPA RATPWRPTGPRSCPLVLGKR MRSWSCWWAALQSCGRQMRG C in isoform 2. 1 PublicationAdd BLAST | 61 | |
Alternative sequenceiVSP_043948 | 98 – 462 | Missing in isoform 2. 1 PublicationAdd BLAST | 365 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC020922 Genomic DNA No translation available. BC005842 mRNA Translation: AAH05842.1 Sequence problems. BC019313 mRNA Translation: AAH19313.1 BC044889 mRNA Translation: AAH44889.1 AF289582 mRNA Translation: AAL55766.1 Sequence problems. |
CCDSi | CCDS12922.1 [Q86Y97-1] |
RefSeqi | NP_116090.2, NM_032701.3 [Q86Y97-1] XP_005259395.1, XM_005259338.3 XP_006723505.1, XM_006723442.3 XP_011525717.1, XM_011527415.2 [Q86Y97-1] |
Genome annotation databases
Ensembli | ENST00000255613; ENSP00000255613; ENSG00000133247 [Q86Y97-1] ENST00000445196; ENSP00000397296; ENSG00000133247 [Q86Y97-2] ENST00000592631; ENSP00000467499; ENSG00000133247 [Q86Y97-2] |
GeneIDi | 84787 |
KEGGi | hsa:84787 |
UCSCi | uc002qkj.5, human [Q86Y97-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC020922 Genomic DNA No translation available. BC005842 mRNA Translation: AAH05842.1 Sequence problems. BC019313 mRNA Translation: AAH19313.1 BC044889 mRNA Translation: AAH44889.1 AF289582 mRNA Translation: AAL55766.1 Sequence problems. |
CCDSi | CCDS12922.1 [Q86Y97-1] |
RefSeqi | NP_116090.2, NM_032701.3 [Q86Y97-1] XP_005259395.1, XM_005259338.3 XP_006723505.1, XM_006723442.3 XP_011525717.1, XM_011527415.2 [Q86Y97-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3RQ4 | X-ray | 1.80 | A | 2-248 | [»] | |
SMRi | Q86Y97 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124257, 12 interactors |
IntActi | Q86Y97, 29 interactors |
MINTi | Q86Y97 |
STRINGi | 9606.ENSP00000255613 |
Chemistry databases
BindingDBi | Q86Y97 |
ChEMBLi | CHEMBL2321644 |
GuidetoPHARMACOLOGYi | 2718 |
PTM databases
iPTMneti | Q86Y97 |
PhosphoSitePlusi | Q86Y97 |
Genetic variation databases
BioMutai | KMT5C |
DMDMi | 74727906 |
Proteomic databases
EPDi | Q86Y97 |
jPOSTi | Q86Y97 |
MassIVEi | Q86Y97 |
MaxQBi | Q86Y97 |
PaxDbi | Q86Y97 |
PeptideAtlasi | Q86Y97 |
PRIDEi | Q86Y97 |
ProteomicsDBi | 70387 [Q86Y97-1] 70388 [Q86Y97-2] |
Protocols and materials databases
ABCDi | Q86Y97, 1 sequenced antibody |
Antibodypediai | 46420, 62 antibodies |
Genome annotation databases
Ensembli | ENST00000255613; ENSP00000255613; ENSG00000133247 [Q86Y97-1] ENST00000445196; ENSP00000397296; ENSG00000133247 [Q86Y97-2] ENST00000592631; ENSP00000467499; ENSG00000133247 [Q86Y97-2] |
GeneIDi | 84787 |
KEGGi | hsa:84787 |
UCSCi | uc002qkj.5, human [Q86Y97-1] |
Organism-specific databases
CTDi | 84787 |
DisGeNETi | 84787 |
GeneCardsi | KMT5C |
HGNCi | HGNC:28405, KMT5C |
HPAi | ENSG00000133247, Low tissue specificity |
MIMi | 613198, gene |
neXtProti | NX_Q86Y97 |
OpenTargetsi | ENSG00000133247 |
PharmGKBi | PA134934307 |
VEuPathDBi | HostDB:ENSG00000133247.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2589, Eukaryota |
GeneTreei | ENSGT00940000161700 |
HOGENOMi | CLU_040002_0_0_1 |
InParanoidi | Q86Y97 |
OMAi | LHRWGGC |
OrthoDBi | 236983at2759 |
PhylomeDBi | Q86Y97 |
TreeFami | TF106433 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS13469-MONOMER |
PathwayCommonsi | Q86Y97 |
Reactomei | R-HSA-3214841, PKMTs methylate histone lysines |
Miscellaneous databases
BioGRID-ORCSi | 84787, 5 hits in 882 CRISPR screens |
ChiTaRSi | KMT5C, human |
GenomeRNAii | 84787 |
Pharosi | Q86Y97, Tchem |
PROi | PR:Q86Y97 |
RNActi | Q86Y97, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000133247, Expressed in layer of synovial tissue and 220 other tissues |
ExpressionAtlasi | Q86Y97, baseline and differential |
Genevisiblei | Q86Y97, HS |
Family and domain databases
Gene3Di | 1.10.10.1700, 1 hit |
InterProi | View protein in InterPro IPR041938, Hist-Lys_N-MTase_N IPR025790, Hist-Lys_N-MTase_Suvar4-20 IPR039977, KMT5B/KMT5C/SET9 IPR001214, SET_dom |
PANTHERi | PTHR12977, PTHR12977, 1 hit |
Pfami | View protein in Pfam PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51570, SAM_MT43_SUVAR420_2, 1 hit PS50280, SET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KMT5C_HUMAN | |
Accessioni | Q86Y97Primary (citable) accession number: Q86Y97 Secondary accession number(s): Q8WZ10, Q9BRZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families