UniProtKB - Q86XT4 (TRI50_HUMAN)
Protein
E3 ubiquitin-protein ligase TRIM50
Gene
TRIM50
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.3 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 84 – 125 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- ubiquitin protein ligase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- protein ubiquitination Source: GO_Central
- regulation of establishment of protein localization Source: Ensembl
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 6.3.2.19, 2681 |
PathwayCommonsi | Q86XT4 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM50 (EC:2.3.2.271 Publication)Alternative name(s): RING-type E3 ubiquitin transferase TRIM50Curated Tripartite motif-containing protein 50 |
Gene namesi | Name:TRIM50 Synonyms:TRIM50A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19017, TRIM50 |
MIMi | 612548, gene |
neXtProti | NX_Q86XT4 |
VEuPathDBi | HostDB:ENSG00000146755.10 |
Subcellular locationi
Other locations
- Cytoplasm 3 Publications
Note: Localizes mainly into discrete cytoplasmic punctuate structures heterogeneous in size and shape containing polyubiquitinated proteins.1 Publication
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 135892 |
PharmGKBi | PA38778 |
Miscellaneous databases
Pharosi | Q86XT4, Tdark |
Genetic variation databases
BioMutai | TRIM50 |
DMDMi | 56404881 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056274 | 1 – 487 | E3 ubiquitin-protein ligase TRIM50Add BLAST | 487 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 373 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Auto-ubiquitinated.1 Publication
Acetylated by EP300 and KAT2B. HDAC6 drives TRIM50 deacetylation. Acetylation antagonizes with TRIM50 ubiquitination.1 Publication
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
jPOSTi | Q86XT4 |
PaxDbi | Q86XT4 |
PRIDEi | Q86XT4 |
ProteomicsDBi | 70331 [Q86XT4-1] 70332 [Q86XT4-2] |
PTM databases
iPTMneti | Q86XT4 |
PhosphoSitePlusi | Q86XT4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000146755, Expressed in body of stomach and 93 other tissues |
ExpressionAtlasi | Q86XT4, baseline and differential |
Genevisiblei | Q86XT4, HS |
Organism-specific databases
HPAi | ENSG00000146755, Group enriched (pancreas, stomach) |
Interactioni
Subunit structurei
Can form dimers and trimers.
Interacts with several E2 ubiquitin-conjugating enzymes, including UBE2L6, UBE2E1, UBE2E3. No interaction with UBE2H.
Interacts with BECN1.
Interacts with SQSTM1.
3 PublicationsBinary interactionsi
Hide detailsQ86XT4
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 126435, 44 interactors |
IntActi | Q86XT4, 35 interactors |
STRINGi | 9606.ENSP00000327994 |
Miscellaneous databases
RNActi | Q86XT4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 276 – 475 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 200 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 125 – 169 | Sequence analysisAdd BLAST | 45 | |
Coiled coili | 204 – 235 | Sequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 84 – 125 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
HOGENOMi | CLU_013137_0_3_1 |
InParanoidi | Q86XT4 |
OrthoDBi | 574602at2759 |
PhylomeDBi | Q86XT4 |
TreeFami | TF342569 |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR003877, SPRY_dom IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform Alpha (identifier: Q86XT4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAWQVSLLEL EDWLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE
60 70 80 90 100
LRCPVCRQAV DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC
110 120 130 140 150
EKDQELICGL CGLLGSHQHH PVTPVSTVYS RMKEELAALI SELKQEQKKV
160 170 180 190 200
DELIAKLVNN RTRIVNESDV FSWVIRREFQ ELHHLVDEEK ARCLEGIGGH
210 220 230 240 250
TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF IRKFHSMASR
260 270 280 290 300
AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP
310 320 330 340 350
ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY
360 370 380 390 400
WEVVVGSKSD WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC
410 420 430 440 450
PRVPLPVAGH PHRIGLYLHY EQGELTFFDA DRPDDLRPLY TFQADFQGKL
460 470 480
YPILDTCWHE RGSNSLPMVL PPPSGPGPLS PEQPTKL
Isoform Beta (identifier: Q86XT4-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
243-243: Missing.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020491 | 8 | L → P. Corresponds to variant dbSNP:rs6980124Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012088 | 243 | Missing in isoform Beta. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY081948 mRNA Translation: AAL91071.1 AY081949 mRNA Translation: AAL91072.1 AC073841 Genomic DNA No translation available. |
CCDSi | CCDS34654.1 [Q86XT4-1] |
RefSeqi | NP_001268379.1, NM_001281450.1 NP_001268380.1, NM_001281451.1 NP_835226.2, NM_178125.3 |
Genome annotation databases
Ensembli | ENST00000333149; ENSP00000327994; ENSG00000146755 ENST00000453152; ENSP00000413875; ENSG00000146755 |
GeneIDi | 135892 |
KEGGi | hsa:135892 |
UCSCi | uc032zrf.2, human [Q86XT4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY081948 mRNA Translation: AAL91071.1 AY081949 mRNA Translation: AAL91072.1 AC073841 Genomic DNA No translation available. |
CCDSi | CCDS34654.1 [Q86XT4-1] |
RefSeqi | NP_001268379.1, NM_001281450.1 NP_001268380.1, NM_001281451.1 NP_835226.2, NM_178125.3 |
3D structure databases
SMRi | Q86XT4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 126435, 44 interactors |
IntActi | Q86XT4, 35 interactors |
STRINGi | 9606.ENSP00000327994 |
PTM databases
iPTMneti | Q86XT4 |
PhosphoSitePlusi | Q86XT4 |
Genetic variation databases
BioMutai | TRIM50 |
DMDMi | 56404881 |
Proteomic databases
jPOSTi | Q86XT4 |
PaxDbi | Q86XT4 |
PRIDEi | Q86XT4 |
ProteomicsDBi | 70331 [Q86XT4-1] 70332 [Q86XT4-2] |
Protocols and materials databases
Antibodypediai | 14287, 130 antibodies |
Genome annotation databases
Ensembli | ENST00000333149; ENSP00000327994; ENSG00000146755 ENST00000453152; ENSP00000413875; ENSG00000146755 |
GeneIDi | 135892 |
KEGGi | hsa:135892 |
UCSCi | uc032zrf.2, human [Q86XT4-1] |
Organism-specific databases
CTDi | 135892 |
DisGeNETi | 135892 |
GeneCardsi | TRIM50 |
HGNCi | HGNC:19017, TRIM50 |
HPAi | ENSG00000146755, Group enriched (pancreas, stomach) |
MIMi | 612548, gene |
neXtProti | NX_Q86XT4 |
PharmGKBi | PA38778 |
VEuPathDBi | HostDB:ENSG00000146755.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
HOGENOMi | CLU_013137_0_3_1 |
InParanoidi | Q86XT4 |
OrthoDBi | 574602at2759 |
PhylomeDBi | Q86XT4 |
TreeFami | TF342569 |
Enzyme and pathway databases
BRENDAi | 6.3.2.19, 2681 |
PathwayCommonsi | Q86XT4 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 135892, 7 hits in 871 CRISPR screens |
ChiTaRSi | TRIM50, human |
GenomeRNAii | 135892 |
Pharosi | Q86XT4, Tdark |
PROi | PR:Q86XT4 |
RNActi | Q86XT4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000146755, Expressed in body of stomach and 93 other tissues |
ExpressionAtlasi | Q86XT4, baseline and differential |
Genevisiblei | Q86XT4, HS |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR003877, SPRY_dom IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI50_HUMAN | |
Accessioni | Q86XT4Primary (citable) accession number: Q86XT4 Secondary accession number(s): Q86XT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants