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UniProtKB - Q86XP1 (DGKH_HUMAN)

Protein

Diacylglycerol kinase eta

Gene

DGKH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 225Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: InterPro
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis

Chemistry databases

SwissLipidsiSLP:000000928 [Q86XP1-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase eta (EC:2.7.1.107)
Short name:
DAG kinase eta
Alternative name(s):
Diglyceride kinase eta
Short name:
DGK-eta
Gene namesi
Name:DGKH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000102780.16
HGNCiHGNC:2854 DGKH
MIMi604071 gene
neXtProtiNX_Q86XP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1151W → G: Abolishes homo and heterooligomerization but not its catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi160851
OpenTargetsiENSG00000102780
PharmGKBiPA27315

Polymorphism and mutation databases

BioMutaiDGKH
DMDMi74762463

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393671 – 1220Diacylglycerol kinase etaAdd BLAST1220

Post-translational modificationi

Phosphorylated; does not inhibit catalytic activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86XP1
MaxQBiQ86XP1
PaxDbiQ86XP1
PeptideAtlasiQ86XP1
PRIDEiQ86XP1
ProteomicsDBi70309
70310 [Q86XP1-2]
70311 [Q86XP1-3]

PTM databases

iPTMnetiQ86XP1
PhosphoSitePlusiQ86XP1

Expressioni

Tissue specificityi

Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.1 Publication

Gene expression databases

BgeeiENSG00000102780 Expressed in 191 organ(s), highest expression level in dorsal root ganglion
CleanExiHS_DGKH
ExpressionAtlasiQ86XP1 baseline and differential
GenevisibleiQ86XP1 HS

Organism-specific databases

HPAiHPA039533
HPA040355

Interactioni

Subunit structurei

Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF.2 Publications

Protein-protein interaction databases

BioGridi127768, 12 interactors
IntActiQ86XP1, 5 interactors
STRINGi9606.ENSP00000337572

Structurei

3D structure databases

ProteinModelPortaliQ86XP1
SMRiQ86XP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 158PHPROSITE-ProRule annotationAdd BLAST94
Domaini328 – 463DAGKcPROSITE-ProRule annotationAdd BLAST136
Domaini1151 – 1214SAMPROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1218 – 1220PDZ-binding3

Sequence similaritiesi

Belongs to the eukaryotic diacylglycerol kinase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 225Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1170 Eukaryota
ENOG410XSJR LUCA
GeneTreeiENSGT00760000119050
HOVERGENiHBG051346
InParanoidiQ86XP1
KOiK00901
OMAiHLYIQHA
OrthoDBiEOG091G00SK
PhylomeDBiQ86XP1
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.30, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001660 SAM
IPR013761 SAM/pointed_sf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00169 PH, 1 hit
PF07647 SAM_2, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00233 PH, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50105 SAM_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86XP1-1) [UniParc]FASTAAdd to basket
Also known as: DAG kinase eta-2, DGKH-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK 
        60         70         80         90        100
LIRKVSTSGQ IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS 
       110        120        130        140        150
KSLIFDEVDL SDASVAEAST KNANNSFTII TPFRRLMLCA ENRKEMEDWI 
       160        170        180        190        200
SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA CSHARPTFCN VCRESLSGVT 
       210        220        230        240        250
SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED EDGVAMPHQW 
       260        270        280        290        300
LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL 
       310        320        330        340        350
GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL 
       360        370        380        390        400
RRFKQLLNPA QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE 
       410        420        430        440        450
IDKLNLNKQC QLGVLPLGTG NDLARVLGWG GSYDDDTQLP QILEKLERAS 
       460        470        480        490        500
TKMLDRWSIM TYELKLPPKA SLLPGPPEAS EEFYMTIYED SVATHLTKIL 
       510        520        530        540        550
NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA DAVASKCSVL 
       560        570        580        590        600
NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG 
       610        620        630        640        650
DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA 
       660        670        680        690        700
NQSSDYDSTE TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV 
       710        720        730        740        750
PGPAVAASKE NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG 
       760        770        780        790        800
ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS 
       810        820        830        840        850
RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI 
       860        870        880        890        900
PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH 
       910        920        930        940        950
HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR 
       960        970        980        990       1000
AFESTLKSWE DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE 
      1010       1020       1030       1040       1050
ELITRICDAA TIHCLLEQEL AHAVNACSHA LNKANPRCPE SLTRDTATEI 
      1060       1070       1080       1090       1100
AINVKALYNE TESLLVGRVP LQLESPHEER VSNALHSVEV ELQKLTEIPW 
      1110       1120       1130       1140       1150
LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK QKTSSQPVQK 
      1160       1170       1180       1190       1200
WGTEEVAAWL DLLNLGEYKD IFIRHDIRGA ELLHLERRDL KDLGIPKVGH 
      1210       1220
VKRILQGIKE LGRSTPQSEV
Length:1,220
Mass (Da):134,866
Last modified:June 1, 2003 - v1
Checksum:iF1BBF5B7FA582C97
GO
Isoform 2 (identifier: Q86XP1-2) [UniParc]FASTAAdd to basket
Also known as: DAG kinase eta-1, DGKH-1

The sequence of this isoform differs from the canonical sequence as follows:
     1148-1220: VQKWGTEEVA...LGRSTPQSEV → GSGDTESGSCEANSPGN

Show »
Length:1,164
Mass (Da):128,035
Checksum:iDF39B04BECC0B629
GO
Isoform 3 (identifier: Q86XP1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.
     1191-1191: K → KNTVGEKRDTKENGKHM

Note: No experimental confirmation available.
Show »
Length:1,100
Mass (Da):122,392
Checksum:i0D36C1FA1E7C1A43
GO
Isoform 4 (identifier: Q86XP1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Show »
Length:1,084
Mass (Da):120,566
Checksum:i8994FBC10F6DB99D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0D9SFR6A0A0D9SFR6_HUMAN
Diacylglycerol kinase
DGKH
1,028Annotation score:
A0A0D9SF65A0A0D9SF65_HUMAN
Diacylglycerol kinase eta
DGKH
89Annotation score:
A0A087WZ02A0A087WZ02_HUMAN
Diacylglycerol kinase eta
DGKH
62Annotation score:

Sequence cautioni

The sequence BAC05280 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti677I → M in BAC05280 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0338671201V → A. Corresponds to variant dbSNP:rs17646069Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0191831 – 136Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST136
Alternative sequenceiVSP_0191851148 – 1220VQKWG…PQSEV → GSGDTESGSCEANSPGN in isoform 2. 2 PublicationsAdd BLAST73
Alternative sequenceiVSP_0191861191K → KNTVGEKRDTKENGKHM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078967 mRNA Translation: BAC66960.1
AB078968 mRNA Translation: BAC66961.1
AK302727 mRNA Translation: BAG63946.1
AL136527 Genomic DNA No translation available.
AL139328 Genomic DNA No translation available.
AL157932 Genomic DNA No translation available.
BC043292 mRNA Translation: AAH43292.1
AL833883 mRNA Translation: CAD38739.1
AK098302 mRNA Translation: BAC05280.1 Different initiation.
CCDSiCCDS55898.1 [Q86XP1-3]
CCDS55899.1 [Q86XP1-5]
CCDS9381.1 [Q86XP1-1]
CCDS9382.1 [Q86XP1-2]
RefSeqiNP_001191433.1, NM_001204504.2 [Q86XP1-2]
NP_001191434.1, NM_001204505.2 [Q86XP1-3]
NP_001191435.1, NM_001204506.2 [Q86XP1-5]
NP_001284358.1, NM_001297429.1
NP_690874.2, NM_152910.5 [Q86XP1-2]
NP_821077.1, NM_178009.4 [Q86XP1-1]
UniGeneiHs.630380
Hs.659437
Hs.733239

Genome annotation databases

EnsembliENST00000261491; ENSP00000261491; ENSG00000102780 [Q86XP1-2]
ENST00000337343; ENSP00000337572; ENSG00000102780 [Q86XP1-1]
ENST00000379274; ENSP00000368576; ENSG00000102780 [Q86XP1-2]
ENST00000536612; ENSP00000445114; ENSG00000102780 [Q86XP1-3]
ENST00000628433; ENSP00000485809; ENSG00000102780 [Q86XP1-5]
GeneIDi160851
KEGGihsa:160851
UCSCiuc001uyl.3 human [Q86XP1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078967 mRNA Translation: BAC66960.1
AB078968 mRNA Translation: BAC66961.1
AK302727 mRNA Translation: BAG63946.1
AL136527 Genomic DNA No translation available.
AL139328 Genomic DNA No translation available.
AL157932 Genomic DNA No translation available.
BC043292 mRNA Translation: AAH43292.1
AL833883 mRNA Translation: CAD38739.1
AK098302 mRNA Translation: BAC05280.1 Different initiation.
CCDSiCCDS55898.1 [Q86XP1-3]
CCDS55899.1 [Q86XP1-5]
CCDS9381.1 [Q86XP1-1]
CCDS9382.1 [Q86XP1-2]
RefSeqiNP_001191433.1, NM_001204504.2 [Q86XP1-2]
NP_001191434.1, NM_001204505.2 [Q86XP1-3]
NP_001191435.1, NM_001204506.2 [Q86XP1-5]
NP_001284358.1, NM_001297429.1
NP_690874.2, NM_152910.5 [Q86XP1-2]
NP_821077.1, NM_178009.4 [Q86XP1-1]
UniGeneiHs.630380
Hs.659437
Hs.733239

3D structure databases

ProteinModelPortaliQ86XP1
SMRiQ86XP1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127768, 12 interactors
IntActiQ86XP1, 5 interactors
STRINGi9606.ENSP00000337572

Chemistry databases

SwissLipidsiSLP:000000928 [Q86XP1-2]

PTM databases

iPTMnetiQ86XP1
PhosphoSitePlusiQ86XP1

Polymorphism and mutation databases

BioMutaiDGKH
DMDMi74762463

Proteomic databases

EPDiQ86XP1
MaxQBiQ86XP1
PaxDbiQ86XP1
PeptideAtlasiQ86XP1
PRIDEiQ86XP1
ProteomicsDBi70309
70310 [Q86XP1-2]
70311 [Q86XP1-3]

Protocols and materials databases

DNASUi160851
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261491; ENSP00000261491; ENSG00000102780 [Q86XP1-2]
ENST00000337343; ENSP00000337572; ENSG00000102780 [Q86XP1-1]
ENST00000379274; ENSP00000368576; ENSG00000102780 [Q86XP1-2]
ENST00000536612; ENSP00000445114; ENSG00000102780 [Q86XP1-3]
ENST00000628433; ENSP00000485809; ENSG00000102780 [Q86XP1-5]
GeneIDi160851
KEGGihsa:160851
UCSCiuc001uyl.3 human [Q86XP1-1]

Organism-specific databases

CTDi160851
DisGeNETi160851
EuPathDBiHostDB:ENSG00000102780.16
GeneCardsiDGKH
HGNCiHGNC:2854 DGKH
HPAiHPA039533
HPA040355
MIMi604071 gene
neXtProtiNX_Q86XP1
OpenTargetsiENSG00000102780
PharmGKBiPA27315
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1170 Eukaryota
ENOG410XSJR LUCA
GeneTreeiENSGT00760000119050
HOVERGENiHBG051346
InParanoidiQ86XP1
KOiK00901
OMAiHLYIQHA
OrthoDBiEOG091G00SK
PhylomeDBiQ86XP1
TreeFamiTF313104

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis

Miscellaneous databases

ChiTaRSiDGKH human
GenomeRNAii160851
PROiPR:Q86XP1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102780 Expressed in 191 organ(s), highest expression level in dorsal root ganglion
CleanExiHS_DGKH
ExpressionAtlasiQ86XP1 baseline and differential
GenevisibleiQ86XP1 HS

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.30, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001660 SAM
IPR013761 SAM/pointed_sf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00169 PH, 1 hit
PF07647 SAM_2, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00233 PH, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50105 SAM_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDGKH_HUMAN
AccessioniPrimary (citable) accession number: Q86XP1
Secondary accession number(s): A2A2W7
, A6NFX7, B4DZ34, Q5VZW0, Q6PI56, Q86XP2, Q8N3N0, Q8N7J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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