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Entry version 138 (07 Apr 2021)
Sequence version 1 (01 Jun 2003)
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Protein

Dihydrofolate reductase 2, mitochondrial

Gene

DHFR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.2 Publications

Miscellaneous

Humans have acquired two dihydrofolate reductase enzymes during their evolution, DHFR and DHFR2. In contrast to human, mice and brown rats have just one.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=209 µM for dihydrofolate1 Publication
  2. KM=20 µM for NADPH1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
    Proteins known to be involved in this subpathway in this organism are:
    1. Dihydrofolate reductase (DHFR), Dihydrofolate reductase (DYR), Dihydrofolate reductase 2, mitochondrial (DHFR2), Dihydrofolate reductase (DHFR), Dihydrofolate reductase (DHFR)
    This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei10NADP; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei71SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi16 – 22NADPBy similarity7
    Nucleotide bindingi55 – 57NADPBy similarity3
    Nucleotide bindingi77 – 79NADPBy similarity3
    Nucleotide bindingi117 – 124NADPBy similarity8

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    • dihydrofolate reductase activity Source: UniProtKB
    • mRNA binding Source: UniProtKB
    • NADP binding Source: GO_Central

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    Biological processOne-carbon metabolism
    LigandNADP

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:ENSG00000178700-MONOMER

    Pathway Commons web resource for biological pathway data

    More...
    PathwayCommonsi
    Q86XF0

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-HSA-196757, Metabolism of folate and pterines

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00077;UER00158

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Dihydrofolate reductase 2, mitochondrial
    Alternative name(s):
    Dihydrofolate reductase, mitochondrial (EC:1.5.1.3)
    Dihydrofolate reductase-like protein 1
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:DHFR2Imported
    Synonyms:DHFRL1, DHFRP4
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

    Organism-specific databases

    Human Gene Nomenclature Database

    More...
    HGNCi
    HGNC:27309, DHFR2

    Online Mendelian Inheritance in Man (OMIM)

    More...
    MIMi
    616588, gene

    neXtProt; the human protein knowledge platform

    More...
    neXtProti
    NX_Q86XF0

    Eukaryotic Pathogen, Vector and Host Database Resources

    More...
    VEuPathDBi
    HostDB:ENSG00000178700.7

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Organism-specific databases

    DisGeNET

    More...
    DisGeNETi
    200895

    Open Targets

    More...
    OpenTargetsi
    ENSG00000178700

    The Pharmacogenetics and Pharmacogenomics Knowledge Base

    More...
    PharmGKBi
    PA134889916

    Miscellaneous databases

    Pharos NIH Druggable Genome Knowledgebase

    More...
    Pharosi
    Q86XF0, Tbio

    Chemistry databases

    Drug and drug target database

    More...
    DrugBanki
    DB02402, 5-(4-Methoxyphenoxy)-2,4-Quinazolinediamine
    DB02001, 5-(4-Morpholin-4-Yl-Phenylsulfanyl)-2,4-Quinazolinediamine
    DB04306, 5-[(4-Methylphenyl)Sulfanyl]-2,4-Quinazolinediamine
    DB01958, 5-[4-Tert-Butylphenylsulfanyl]-2,4-Quinazolinediamine
    DB01929, 5-Chloryl-2,4,6-quinazolinetriamine
    DB04163, 5-Phenylsulfanyl-2,4-Quinazolinediamine
    DB07862, 7-(1-ETHYL-PROPYL)-7H-PYRROLO-[3,2-F]QUINAZOLINE-1,3-DIAMINE
    DB08203, 7-[2-METHOXY-1-(METHOXYMETHYL)ETHYL]-7H-PYRROLO[3,2-F] QUINAZOLINE-1,3-DIAMINE
    DB12116, Epigallocatechin gallate

    Genetic variation databases

    BioMuta curated single-nucleotide variation and disease association database

    More...
    BioMutai
    DHFR2

    Domain mapping of disease mutations (DMDM)

    More...
    DMDMi
    74727819

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001863681 – 187Dihydrofolate reductase 2, mitochondrialAdd BLAST187

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    Q86XF0

    MassIVE - Mass Spectrometry Interactive Virtual Environment

    More...
    MassIVEi
    Q86XF0

    MaxQB - The MaxQuant DataBase

    More...
    MaxQBi
    Q86XF0

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q86XF0

    PeptideAtlas

    More...
    PeptideAtlasi
    Q86XF0

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q86XF0

    ProteomicsDB: a multi-organism proteome resource

    More...
    ProteomicsDBi
    70273

    Consortium for Top Down Proteomics

    More...
    TopDownProteomicsi
    Q86XF0

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    Q86XF0

    Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

    More...
    PhosphoSitePlusi
    Q86XF0

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Expressed in numerous cell lines.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    ENSG00000178700, Expressed in buccal mucosa cell and 222 other tissues

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    Q86XF0, baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    Q86XF0, HS

    Organism-specific databases

    Human Protein Atlas

    More...
    HPAi
    ENSG00000178700, Low tissue specificity

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    128354, 62 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    Q86XF0, 25 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    9606.ENSP00000377768

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...
    RNActi
    Q86XF0, protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q86XF0

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini4 – 185DHFRPROSITE-ProRule annotationAdd BLAST182

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni31 – 36Substrate bindingBy similarity6

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG1324, Eukaryota

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00390000010283

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q86XF0

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    QEGKHIK

    Database of Orthologous Groups

    More...
    OrthoDBi
    1489621at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q86XF0

    TreeFam database of animal gene trees

    More...
    TreeFami
    TF317636

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00209, DHFR, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.430.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR012259, DHFR
    IPR024072, DHFR-like_dom_sf
    IPR001796, DHFR_dom

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR48069, PTHR48069, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00186, DHFR_1, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF53597, SSF53597, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS51330, DHFR_2, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

    Q86XF0-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MFLLLNCIVA VSQNMGIGKN GDLPRPPLRN EFRYFQRMTT TSSVEGKQNL
    60 70 80 90 100
    VIMGRKTWFS IPEKNRPLKD RINLVLSREL KEPPQGAHFL ARSLDDALKL
    110 120 130 140 150
    TERPELANKV DMIWIVGGSS VYKEAMNHLG HLKLFVTRIM QDFESDTFFS
    160 170 180
    EIDLEKYKLL PEYPGVLSDV QEGKHIKYKF EVCEKDD
    Length:187
    Mass (Da):21,620
    Last modified:June 1, 2003 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBA6548FB0F576FF7
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    C9JJ68C9JJ68_HUMAN
    Dihydrofolate reductase
    DHFR2
    114Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_061135166V → I1 PublicationCorresponds to variant dbSNP:rs17855824Ensembl.1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AL832912 mRNA Translation: CAH10617.1
    AC130896 Genomic DNA No translation available.
    CH471052 Genomic DNA Translation: EAW79895.1
    CH471052 Genomic DNA Translation: EAW79896.1
    BC045541 mRNA Translation: AAH45541.1
    BC063379 mRNA Translation: AAH63379.1

    The Consensus CDS (CCDS) project

    More...
    CCDSi
    CCDS2926.1

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001182572.1, NM_001195643.1
    NP_789785.1, NM_176815.4
    XP_011510839.1, XM_011512537.2

    Genome annotation databases

    Ensembl eukaryotic genome annotation project

    More...
    Ensembli
    ENST00000314636; ENSP00000319170; ENSG00000178700
    ENST00000394221; ENSP00000377768; ENSG00000178700
    ENST00000461173; ENSP00000418415; ENSG00000178700
    ENST00000619045; ENSP00000480823; ENSG00000178700

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    200895

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    hsa:200895

    UCSC genome browser

    More...
    UCSCi
    uc003dri.3, human

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL832912 mRNA Translation: CAH10617.1
    AC130896 Genomic DNA No translation available.
    CH471052 Genomic DNA Translation: EAW79895.1
    CH471052 Genomic DNA Translation: EAW79896.1
    BC045541 mRNA Translation: AAH45541.1
    BC063379 mRNA Translation: AAH63379.1
    CCDSiCCDS2926.1
    RefSeqiNP_001182572.1, NM_001195643.1
    NP_789785.1, NM_176815.4
    XP_011510839.1, XM_011512537.2

    3D structure databases

    SMRiQ86XF0
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi128354, 62 interactors
    IntActiQ86XF0, 25 interactors
    STRINGi9606.ENSP00000377768

    Chemistry databases

    DrugBankiDB02402, 5-(4-Methoxyphenoxy)-2,4-Quinazolinediamine
    DB02001, 5-(4-Morpholin-4-Yl-Phenylsulfanyl)-2,4-Quinazolinediamine
    DB04306, 5-[(4-Methylphenyl)Sulfanyl]-2,4-Quinazolinediamine
    DB01958, 5-[4-Tert-Butylphenylsulfanyl]-2,4-Quinazolinediamine
    DB01929, 5-Chloryl-2,4,6-quinazolinetriamine
    DB04163, 5-Phenylsulfanyl-2,4-Quinazolinediamine
    DB07862, 7-(1-ETHYL-PROPYL)-7H-PYRROLO-[3,2-F]QUINAZOLINE-1,3-DIAMINE
    DB08203, 7-[2-METHOXY-1-(METHOXYMETHYL)ETHYL]-7H-PYRROLO[3,2-F] QUINAZOLINE-1,3-DIAMINE
    DB12116, Epigallocatechin gallate

    PTM databases

    iPTMnetiQ86XF0
    PhosphoSitePlusiQ86XF0

    Genetic variation databases

    BioMutaiDHFR2
    DMDMi74727819

    Proteomic databases

    jPOSTiQ86XF0
    MassIVEiQ86XF0
    MaxQBiQ86XF0
    PaxDbiQ86XF0
    PeptideAtlasiQ86XF0
    PRIDEiQ86XF0
    ProteomicsDBi70273
    TopDownProteomicsiQ86XF0

    Protocols and materials databases

    Antibodypedia a portal for validated antibodies

    More...
    Antibodypediai
    32082, 121 antibodies

    The DNASU plasmid repository

    More...
    DNASUi
    200895

    Genome annotation databases

    EnsembliENST00000314636; ENSP00000319170; ENSG00000178700
    ENST00000394221; ENSP00000377768; ENSG00000178700
    ENST00000461173; ENSP00000418415; ENSG00000178700
    ENST00000619045; ENSP00000480823; ENSG00000178700
    GeneIDi200895
    KEGGihsa:200895
    UCSCiuc003dri.3, human

    Organism-specific databases

    Comparative Toxicogenomics Database

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    CTDi
    200895
    DisGeNETi200895

    GeneCards: human genes, protein and diseases

    More...
    GeneCardsi
    DHFR2
    HGNCiHGNC:27309, DHFR2
    HPAiENSG00000178700, Low tissue specificity
    MIMi616588, gene
    neXtProtiNX_Q86XF0
    OpenTargetsiENSG00000178700
    PharmGKBiPA134889916
    VEuPathDBiHostDB:ENSG00000178700.7

    GenAtlas: human gene database

    More...
    GenAtlasi
    Search...

    Phylogenomic databases

    eggNOGiKOG1324, Eukaryota
    GeneTreeiENSGT00390000010283
    InParanoidiQ86XF0
    OMAiQEGKHIK
    OrthoDBi1489621at2759
    PhylomeDBiQ86XF0
    TreeFamiTF317636

    Enzyme and pathway databases

    UniPathwayiUPA00077;UER00158
    BioCyciMetaCyc:ENSG00000178700-MONOMER
    PathwayCommonsiQ86XF0
    ReactomeiR-HSA-196757, Metabolism of folate and pterines

    Miscellaneous databases

    BioGRID ORCS database of CRISPR phenotype screens

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    BioGRID-ORCSi
    200895, 371 hits in 919 CRISPR screens

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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    ChiTaRSi
    DHFR2, human

    Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

    More...
    GenomeRNAii
    200895
    PharosiQ86XF0, Tbio

    Protein Ontology

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    PROi
    PR:Q86XF0
    RNActiQ86XF0, protein

    The Stanford Online Universal Resource for Clones and ESTs

    More...
    SOURCEi
    Search...

    Gene expression databases

    BgeeiENSG00000178700, Expressed in buccal mucosa cell and 222 other tissues
    ExpressionAtlasiQ86XF0, baseline and differential
    GenevisibleiQ86XF0, HS

    Family and domain databases

    CDDicd00209, DHFR, 1 hit
    Gene3Di3.40.430.10, 1 hit
    InterProiView protein in InterPro
    IPR012259, DHFR
    IPR024072, DHFR-like_dom_sf
    IPR001796, DHFR_dom
    PANTHERiPTHR48069, PTHR48069, 1 hit
    PfamiView protein in Pfam
    PF00186, DHFR_1, 1 hit
    SUPFAMiSSF53597, SSF53597, 1 hit
    PROSITEiView protein in PROSITE
    PS51330, DHFR_2, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDYR2_HUMAN
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q86XF0
    Secondary accession number(s): D3DN30, Q6P4I9
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2003
    Last modified: April 7, 2021
    This is version 138 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with genetic variants
      List of human entries with genetic variants
    3. Human variants curated from literature reports
      Index of human variants curated from literature reports
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families
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