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UniProtKB - Q86W50 (MET16_HUMAN)

Entry version 130 (02 Dec 2020)
Sequence version 2 (24 Nov 2009)
Previous versions | rss
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Protein

RNA N6-adenosine-methyltransferase METTL16

Gene

METTL16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA N6-methyltransferase that methylates adenosine residues at the N6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).6 Publications

Caution

Consequences of N6-methylation of MAT2A transcripts are subject to discussion. According to a report, N6-methylation of MAT2A affects MAT2A mRNA splicing: in presence of S-adenosyl-L-methionine, METTL16 binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, METTL16 binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A (PubMed:28525753). According to a second report, N6-methylation of MAT2A affects MAT2A mRNA stability instead: in presence of S-adenosyl-L-methionine, METTL16 N6-methylates MAT2A mRNA, leading to destabilization and subsequent degradation of MAT2A mRNA (By similarity). Under low S-adenosyl-L-methionine-limiting condition MAT2A mRNA is not methylated, stabilizing it (By similarity).By similarity1 Publication
Stoichiometry of the protein is unclear. According to two reports, the methyltransferase acts as a monomer (PubMed:30197299, PubMed:30197297). According to a another paper, it acts as a homodimer (PubMed:29593291).3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Methyltransferase activity is autoinhibited by the K-loop region that blocks S-adenosyl-L-methionine-binding (PubMed:30197297). Upon activation, K-loop changes conformation, allowing S-adenosyl-L-methionine-binding and subsequent methyltransferase activity (PubMed:30197297).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei82S-adenosyl-L-methionineCombined sources3 Publications1
Binding sitei110S-adenosyl-L-methionine; via carbonyl oxygenCombined sources2 Publications1
Binding sitei114S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei133S-adenosyl-L-methionineCombined sources3 Publications1
Binding sitei164S-adenosyl-L-methionineCombined sources3 Publications1
Binding sitei184S-adenosyl-L-methionineCombined sources2 Publications1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q86W50

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA N6-adenosine-methyltransferase METTL16Curated
Alternative name(s):
Methyltransferase 10 domain-containing protein1 Publication
Methyltransferase-like protein 161 Publication
N6-adenosine-methyltransferase METTL16 (EC:2.1.1.3483 Publications)
U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferaseCurated (EC:2.1.1.3463 Publications)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:METTL161 PublicationImported
Synonyms:METT10D1 PublicationImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Eukaryotic Pathogen and Host Database Resources

More...EuPathDBi		HostDB:ENSG00000127804.12

Human Gene Nomenclature Database

More...HGNCi		HGNC:28484, METTL16

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q86W50

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi5 – 16KSMHA…RYKDK → ASMHAANAYADA: Abolished methyltransferase activity. 1 PublicationAdd BLAST12
Mutagenesisi5K → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi5K → E: Reduced methyltransferase activity. 1 Publication1
Mutagenesisi10R → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi10R → D or E: Reduced methyltransferase activity. 1 Publication1
Mutagenesisi12R → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi14K → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi16K → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi26K → A: Does not affect methyltransferase activity; when associated with A-31. 1 Publication1
Mutagenesisi31K → A: Does not affect methyltransferase activity; when associated with A-26. 1 Publication1
Mutagenesisi39N → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi47K → E: Reduced methyltransferase activity. 1 Publication1
Mutagenesisi82R → A or E: Abolished methyltransferase activity in vitro. 2 Publications1
Mutagenesisi133E → A: Abolished methyltransferase activity in vitro. 1 Publication1
Mutagenesisi163K → A: Increased methyltransferase activity in vitro. 1 Publication1
Mutagenesisi167M → A: Increased methyltransferase activity in vitro. 1 Publication1
Mutagenesisi184N → A: Abolished methyltransferase activity in vitro. 1 Publication1
Mutagenesisi185 – 186PP → AA: Abolishes methyltransferase activity. 2 Publications2
Mutagenesisi187F → G: Abolishes methyltransferase activity. 2 Publications1
Mutagenesisi200 – 204RNPRR → ENPEE: Abolished methyltransferase activity. 1 Publication5
Mutagenesisi202 – 207PRRPPP → ARRAAA: Does not affect methyltransferase activity. 1 Publication6
Mutagenesisi279R → E: Abolished methyltransferase activity. 1 Publication1
Mutagenesisi282R → E: Abolished methyltransferase activity. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		79066

Open Targets

More...OpenTargetsi		ENSG00000127804

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA142671460

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q86W50, Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		METTL16

Domain mapping of disease mutations (DMDM)

More...DMDMi		269849619

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003107671 – 562RNA N6-adenosine-methyltransferase METTL16Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei329PhosphoserineCombined sources1
Modified residuei463PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q86W50

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q86W50

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q86W50

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q86W50

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q86W50

PeptideAtlas

More...PeptideAtlasi		Q86W50

PRoteomics IDEntifications database

More...PRIDEi		Q86W50

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		70116 [Q86W50-1]
70117 [Q86W50-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q86W50

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q86W50

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000127804, Expressed in calcaneal tendon and 229 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q86W50, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q86W50, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000127804, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with MEPCE (PubMed:29051200).

Interacts with LARP7 (PubMed:29051200).

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		122519, 19 interactors

Protein interaction database and analysis system

More...IntActi		Q86W50, 4 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000263092

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q86W50, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1562
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q86W50

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q86W50

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni17 – 20RNA-bindingCombined sources1 Publication4
Regioni163 – 167K-loop1 Publication5
Regioni199 – 211RNA-bindingCombined sources1 PublicationAdd BLAST13
Regioni250 – 254RNA-bindingCombined sources1 Publication5
Regioni277 – 283RNA-bindingCombined sources1 Publication7
Regioni289 – 400VCR 11 PublicationAdd BLAST112
Regioni514 – 562VCR 21 PublicationAdd BLAST49

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The VCR (vertebrate conserved) regions bind the first hairpin of MAT2A mRNAs (PubMed:28525753). The VCR regions interact with the internal stem-loop within U6 snRNAs, inducing the conformational rearrangement of the A43-containing region of U6 snRNA, thereby modifying the RNA structure to become suitable for productive catalysis by the methyltransferase region (PubMed:32266935).2 Publications
The K-loop region occludes the S-adenosyl-L-methionine-binding pocket (PubMed:30197297). Upon activation, conformation of the K-loop changes, allowing S-adenosyl-L-methionine-binding (PubMed:30197297).1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the methyltransferase superfamily. METTL16/RlmF family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2912, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000016694

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_027534_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q86W50

Identification of Orthologs from Complete Genome Data

More...OMAi		VVVEMHW

Database of Orthologous Groups

More...OrthoDBi		1358504at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q86W50

TreeFam database of animal gene trees

More...TreeFami		TF313132

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017182, METTL16/PsiM
IPR010286, METTL16/RlmF
IPR029063, SAM-dependent_MTases

The PANTHER Classification System

More...PANTHERi		PTHR13393, PTHR13393, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF05971, Methyltransf_10, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF037350, Mtase_ZK1128_prd, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53335, SSF53335, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86W50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE 
        60         70         80         90        100
AVRALTCTLL REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK 
       110        120        130        140        150
STLRRGIDIG TGASCIYPLL GATLNGWYFL ATEVDDMCFN YAKKNVEQNN 
       160        170        180        190        200
LSDLIKVVKV PQKTLLMDAL KEESEIIYDF CMCNPPFFAN QLEAKGVNSR 
       210        220        230        240        250
NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK RLRWYSCMLG 
       260        270        280        290        300
KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS 
       310        320        330        340        350
KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL 
       360        370        380        390        400
TDLKVQHKRV PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE 
       410        420        430        440        450
DVIQALEEKK PTPKESGNSQ ELARGPQERT PCGPALREGE AAAVEGPCPS 
       460        470        480        490        500
QESLSQEENP EPTEDERSEE KGGVEVLESC QGSSNGAQDQ EASEQFGSPV 
       510        520        530        540        550
AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN RDLMNQLCTY 
       560 
IRNQIFRLVA VN
Length:562
Mass (Da):63,621
Last modified:November 24, 2009 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i8CF655F19AF572F0
GO
Isoform 2 (identifier: Q86W50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-224: GITEIMAEGGE → DSLEPGRWRLQ
     225-562: Missing.

Show »
Length:224
Mass (Da):25,502
Checksum:i15AB2DF8B5487A54
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L362I3L362_HUMAN
RNA N6-adenosine-methyltransferase ...
METTL16
168Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
I3L4V1I3L4V1_HUMAN
RNA N6-adenosine-methyltransferase ...
METTL16
148Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
I3L3W3I3L3W3_HUMAN
RNA N6-adenosine-methyltransferase ...
METTL16
121Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
K7EKQ8K7EKQ8_HUMAN
RNA N6-adenosine-methyltransferase ...
METTL16
101Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAB55094 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
The sequence CAD89999 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti39N → D in BAB55094 (PubMed:14702039).Curated1
Sequence conflicti214G → A in CAD89999 (PubMed:17974005).Curated1
Sequence conflicti419S → R in BAB55094 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_081134110G → C Found in patients with large intestine cancer; abolished methyltransferase activity. 1 Publication1
Natural variantiVAR_037086479S → N1 PublicationCorresponds to variant dbSNP:rs17834783Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_029340214 – 224GITEIMAEGGE → DSLEPGRWRLQ in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_029341225 – 562Missing in isoform 2. 1 PublicationAdd BLAST338

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AK027410 mRNA Translation: BAB55094.1 Sequence problems.
AL832612 mRNA Translation: CAD89999.2 Frameshift.
AC006435 Genomic DNA No translation available.
AC015799 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90537.1
CH471108 Genomic DNA Translation: EAW90538.1
BC001213 mRNA Translation: AAH01213.1
BC050603 mRNA Translation: AAH50603.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS42232.1 [Q86W50-1]

NCBI Reference Sequences

More...RefSeqi		NP_076991.3, NM_024086.3 [Q86W50-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000263092; ENSP00000263092; ENSG00000127804 [Q86W50-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		79066

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:79066

UCSC genome browser

More...UCSCi		uc002fut.4, human [Q86W50-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027410 mRNA Translation: BAB55094.1 Sequence problems.
AL832612 mRNA Translation: CAD89999.2 Frameshift.
AC006435 Genomic DNA No translation available.
AC015799 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90537.1
CH471108 Genomic DNA Translation: EAW90538.1
BC001213 mRNA Translation: AAH01213.1
BC050603 mRNA Translation: AAH50603.1
CCDSiCCDS42232.1 [Q86W50-1]
RefSeqiNP_076991.3, NM_024086.3 [Q86W50-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H00X-ray2.00A/B/C40-291[»]
6B91X-ray1.94A1-291[»]
6B92X-ray2.10A1-291[»]
6DU4X-ray1.70A1-310[»]
6DU5X-ray3.01A2-310[»]
6GFKX-ray2.30A/B/C41-291[»]
6GFNX-ray2.86A1-291[»]
6GT5X-ray2.45A/B1-291[»]
6M1UX-ray2.79A310-562[»]
SMRiQ86W50
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi122519, 19 interactors
IntActiQ86W50, 4 interactors
STRINGi9606.ENSP00000263092

PTM databases

iPTMnetiQ86W50
PhosphoSitePlusiQ86W50

Polymorphism and mutation databases

BioMutaiMETTL16
DMDMi269849619

Proteomic databases

EPDiQ86W50
jPOSTiQ86W50
MassIVEiQ86W50
MaxQBiQ86W50
PaxDbiQ86W50
PeptideAtlasiQ86W50
PRIDEiQ86W50
ProteomicsDBi70116 [Q86W50-1]
70117 [Q86W50-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		5377, 246 antibodies

Genome annotation databases

EnsembliENST00000263092; ENSP00000263092; ENSG00000127804 [Q86W50-1]
GeneIDi79066
KEGGihsa:79066
UCSCiuc002fut.4, human [Q86W50-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		79066
DisGeNETi79066
EuPathDBiHostDB:ENSG00000127804.12

GeneCards: human genes, protein and diseases

More...GeneCardsi		METTL16
HGNCiHGNC:28484, METTL16
HPAiENSG00000127804, Low tissue specificity
neXtProtiNX_Q86W50
OpenTargetsiENSG00000127804
PharmGKBiPA142671460

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2912, Eukaryota
GeneTreeiENSGT00390000016694
HOGENOMiCLU_027534_0_0_1
InParanoidiQ86W50
OMAiVVVEMHW
OrthoDBi1358504at2759
PhylomeDBiQ86W50
TreeFamiTF313132

Enzyme and pathway databases

PathwayCommonsiQ86W50

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		79066, 644 hits in 843 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		METTL16, human
EvolutionaryTraceiQ86W50

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		79066
PharosiQ86W50, Tbio

Protein Ontology

More...PROi		PR:Q86W50
RNActiQ86W50, protein

Gene expression databases

BgeeiENSG00000127804, Expressed in calcaneal tendon and 229 other tissues
ExpressionAtlasiQ86W50, baseline and differential
GenevisibleiQ86W50, HS

Family and domain databases

InterProiView protein in InterPro
IPR017182, METTL16/PsiM
IPR010286, METTL16/RlmF
IPR029063, SAM-dependent_MTases
PANTHERiPTHR13393, PTHR13393, 1 hit
PfamiView protein in Pfam
PF05971, Methyltransf_10, 1 hit
PIRSFiPIRSF037350, Mtase_ZK1128_prd, 1 hit
SUPFAMiSSF53335, SSF53335, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMET16_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q86W50
Secondary accession number(s): D3DTI8
, Q86TE5, Q96T16, Q9BVG7
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 24, 2009
Last modified: December 2, 2020
This is version 130 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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