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Protein

Protein Hook homolog 3

Gene

HOOK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).By similarity4 Publications

GO - Molecular functioni

  • dynactin binding Source: UniProtKB
  • dynein intermediate chain binding Source: UniProtKB
  • dynein light chain binding Source: UniProtKB
  • dynein light intermediate chain binding Source: GO_Central
  • identical protein binding Source: UniProtKB
  • microtubule binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

SIGNORiQ86VS8

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 3
Short name:
h-hook3
Short name:
hHK3
Gene namesi
Name:HOOK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000168172.8
HGNCiHGNC:23576 HOOK3
MIMi607825 gene
neXtProtiNX_Q86VS8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi84376
OpenTargetsiENSG00000168172
PharmGKBiPA134961305

Polymorphism and mutation databases

BioMutaiHOOK3
DMDMi41688581

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191971 – 718Protein Hook homolog 3Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei693PhosphoserineCombined sources1
Modified residuei707PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ86VS8
MaxQBiQ86VS8
PaxDbiQ86VS8
PeptideAtlasiQ86VS8
PRIDEiQ86VS8
ProteomicsDBi70068

PTM databases

iPTMnetiQ86VS8
PhosphoSitePlusiQ86VS8

Expressioni

Gene expression databases

BgeeiENSG00000168172 Expressed in 212 organ(s), highest expression level in corpus callosum
CleanExiHS_HOOK3
ExpressionAtlasiQ86VS8 baseline and differential
GenevisibleiQ86VS8 HS

Organism-specific databases

HPAiHPA024756

Interactioni

Subunit structurei

Interacts with Salmonella typhimurium spiC (By similarity). Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK2. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect (PubMed:18799622). Interacts with MSR1, and this association is stimulated by ligand binding to MSR1 (PubMed:17237231). Interacts with microtubules (PubMed:11238449). Interacts with dynein intermediate chain and dynactin (DCTN1) (PubMed:25035494). Interacts with CCDC181 (By similarity). Interacts with LRGUK (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MSR1P217574EBI-1777078,EBI-1776976

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124068, 37 interactors
CORUMiQ86VS8
IntActiQ86VS8, 16 interactors
STRINGi9606.ENSP00000305699

Structurei

Secondary structure

1718
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ86VS8
SMRiQ86VS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 126Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Sufficient for interaction with microtubulesAdd BLAST164
Regioni553 – 718Required for association with GolgiAdd BLAST166
Regioni556 – 718Required for interaction with MSR11 PublicationAdd BLAST163

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili167 – 433Sequence analysisAdd BLAST267
Coiled coili462 – 667Sequence analysisAdd BLAST206

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ86VS8
KOiK16536
OMAiDELKCGQ
OrthoDBiEOG091G06O9
PhylomeDBiQ86VS8
TreeFamiTF320231

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q86VS8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSVESLERA ELCESLLTWI QTFNVDAPCQ TVEDLTNGVV MAQVLQKIDP
60 70 80 90 100
AYFDENWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL
110 120 130 140 150
PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV
160 170 180 190 200
MTAIQELMSK ESPVSAGNDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH
210 220 230 240 250
ELDMQVAALQ EEKSSLLAEN QVLMERLNQS DSIEDPNSPA GRRHLQLQTQ
260 270 280 290 300
LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK
310 320 330 340 350
DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN
360 370 380 390 400
TVSLEEELRK ANAARSQLET YKRQVVELQN RLSEESKKAD KLDFEYKRLK
410 420 430 440 450
EKVDSLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES
460 470 480 490 500
SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEGSDNEKIA LLQSLLDDAN
510 520 530 540 550
LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE
560 570 580 590 600
HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LKIEELQEAL RKKEEEMKQM
610 620 630 640 650
EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE
660 670 680 690 700
YEKTKSQREM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ
710
ATSSRRSYPG HVQPATAR
Length:718
Mass (Da):83,126
Last modified:February 2, 2004 - v2
Checksum:i9528EC9C854D39FA
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YDM4H0YDM4_HUMAN
Protein Hook homolog 3
HOOK3
161Annotation score:
H0YE69H0YE69_HUMAN
Protein Hook homolog 3
HOOK3
101Annotation score:
H0YDK4H0YDK4_HUMAN
Protein Hook homolog 3
HOOK3
31Annotation score:

Sequence cautioni

The sequence AAH48304 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348M → V in BAC03473 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035710221Q → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_049363670Y → S. Corresponds to variant dbSNP:rs34131505Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241830 mRNA Translation: AAK29204.1
CH471080 Genomic DNA Translation: EAW63197.1
CH471080 Genomic DNA Translation: EAW63198.1
BC048304 mRNA Translation: AAH48304.1 Sequence problems.
BC056146 mRNA Translation: AAH56146.1
AK090540 mRNA Translation: BAC03473.1
CCDSiCCDS6139.1
RefSeqiNP_115786.1, NM_032410.3
UniGeneiHs.162852

Genome annotation databases

EnsembliENST00000307602; ENSP00000305699; ENSG00000168172
GeneIDi84376
KEGGihsa:84376
UCSCiuc003xpr.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241830 mRNA Translation: AAK29204.1
CH471080 Genomic DNA Translation: EAW63197.1
CH471080 Genomic DNA Translation: EAW63198.1
BC048304 mRNA Translation: AAH48304.1 Sequence problems.
BC056146 mRNA Translation: AAH56146.1
AK090540 mRNA Translation: BAC03473.1
CCDSiCCDS6139.1
RefSeqiNP_115786.1, NM_032410.3
UniGeneiHs.162852

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J8EX-ray1.70A/B1-160[»]
6B9HX-ray1.50A1-160[»]
ProteinModelPortaliQ86VS8
SMRiQ86VS8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124068, 37 interactors
CORUMiQ86VS8
IntActiQ86VS8, 16 interactors
STRINGi9606.ENSP00000305699

PTM databases

iPTMnetiQ86VS8
PhosphoSitePlusiQ86VS8

Polymorphism and mutation databases

BioMutaiHOOK3
DMDMi41688581

Proteomic databases

EPDiQ86VS8
MaxQBiQ86VS8
PaxDbiQ86VS8
PeptideAtlasiQ86VS8
PRIDEiQ86VS8
ProteomicsDBi70068

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307602; ENSP00000305699; ENSG00000168172
GeneIDi84376
KEGGihsa:84376
UCSCiuc003xpr.4 human

Organism-specific databases

CTDi84376
DisGeNETi84376
EuPathDBiHostDB:ENSG00000168172.8
GeneCardsiHOOK3
HGNCiHGNC:23576 HOOK3
HPAiHPA024756
MIMi607825 gene
neXtProtiNX_Q86VS8
OpenTargetsiENSG00000168172
PharmGKBiPA134961305
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ86VS8
KOiK16536
OMAiDELKCGQ
OrthoDBiEOG091G06O9
PhylomeDBiQ86VS8
TreeFamiTF320231

Enzyme and pathway databases

SIGNORiQ86VS8

Miscellaneous databases

ChiTaRSiHOOK3 human
GeneWikiiHOOK3
GenomeRNAii84376
PROiPR:Q86VS8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168172 Expressed in 212 organ(s), highest expression level in corpus callosum
CleanExiHS_HOOK3
ExpressionAtlasiQ86VS8 baseline and differential
GenevisibleiQ86VS8 HS

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOOK3_HUMAN
AccessioniPrimary (citable) accession number: Q86VS8
Secondary accession number(s): D3DSY8, Q8NBH0, Q9BY13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 7, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
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