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Protein

Vacuolar protein-sorting-associated protein 36

Gene

VPS36

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: InterPro
  • protein C-terminus binding Source: UniProtKB
  • ubiquitin binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ELL-associated protein of 45 kDa
ESCRT-II complex subunit VPS36
Gene namesi
Name:VPS36
Synonyms:C13orf9, EAP45
ORF Names:CGI-145
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000136100.12
HGNCiHGNC:20312 VPS36
MIMi610903 gene
neXtProtiNX_Q86VN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10L → D: No effect on interaction with ubiquitin. 1 Publication1
Mutagenesisi67V → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication1
Mutagenesisi68F → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication1
Mutagenesisi70E → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication1

Organism-specific databases

DisGeNETi51028
OpenTargetsiENSG00000136100
PharmGKBiPA134990943

Polymorphism and mutation databases

DMDMi73920464

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152221 – 386Vacuolar protein-sorting-associated protein 36Add BLAST386

Proteomic databases

EPDiQ86VN1
MaxQBiQ86VN1
PaxDbiQ86VN1
PeptideAtlasiQ86VN1
PRIDEiQ86VN1
ProteomicsDBi70043
70044 [Q86VN1-2]

PTM databases

iPTMnetiQ86VN1
PhosphoSitePlusiQ86VN1

Expressioni

Gene expression databases

BgeeiENSG00000136100 Expressed in 200 organ(s), highest expression level in upper arm skin
CleanExiHS_VPS36
GenevisibleiQ86VN1 HS

Organism-specific databases

HPAiHPA039734
HPA043947

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844). Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118). Interacts (via GLUE domain) with ubiquitin (PubMed:17057716). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938). Interacts with ECPAS (PubMed:20682791).By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNF8Q96H204EBI-4401822,EBI-747719

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119234, 43 interactors
CORUMiQ86VN1
DIPiDIP-29249N
IntActiQ86VN1, 6 interactors
MINTiQ86VN1
STRINGi9606.ENSP00000367299

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ86VN1
SMRiQ86VN1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86VN1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88GLUE N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini105 – 138GLUE C-terminalPROSITE-ProRule annotationAdd BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili160 – 185Sequence analysisAdd BLAST26

Domaini

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.By similarity

Sequence similaritiesi

Belongs to the VPS36 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2760 Eukaryota
ENOG410XR74 LUCA
GeneTreeiENSGT00390000017209
HOVERGENiHBG083632
InParanoidiQ86VN1
KOiK12190
OMAiRKTRIIM
OrthoDBiEOG091G09HR
PhylomeDBiQ86VN1
TreeFamiTF314770

Family and domain databases

Gene3Di1.10.10.10, 2 hits
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR021648 GLUE_dom
IPR011993 PH-like_dom_sf
IPR037855 Vps36
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13128 PTHR13128, 1 hit
PfamiView protein in Pfam
PF11605 Vps36_ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
PROSITEiView protein in PROSITE
PS51495 GLUE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q86VN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDRFVWTSGL LEINETLVIQ QRGVRIYDGE EKIKFDAGTL LLSTHRLIWR
60 70 80 90 100
DQKNHECCMA ILLSQIVFIE EQAAGIGKSA KIVVHLHPAP PNKEPGPFQS
110 120 130 140 150
SKNSYIKLSF KEHGQIEFYR RLSEEMTQRR WENMPVSQSL QTNRGPQPGR
160 170 180 190 200
IRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN
210 220 230 240 250
KIKDKQGDIT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA
260 270 280 290 300
GILQVPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP
310 320 330 340 350
LRLRVFDSGV MVIELQSHKE EEMVASALET VSEKGSLTSE EFAKLVGMSV
360 370 380
LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQS
Length:386
Mass (Da):43,817
Last modified:June 1, 2003 - v1
Checksum:i21E1E66F71BA7764
GO
Isoform 2 (identifier: Q86VN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Note: No experimental confirmation available.
Show »
Length:328
Mass (Da):36,958
Checksum:iF6078BBD25CC0032
GO

Sequence cautioni

The sequence BAB14451 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185M → V in CAI45953 (PubMed:17974005).Curated1
Sequence conflicti223S → R in AAD34140 (PubMed:10810093).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153421 – 58Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151903 mRNA Translation: AAD34140.1
AK023182 mRNA Translation: BAB14451.1 Different initiation.
AK289740 mRNA Translation: BAF82429.1
CR933653 mRNA Translation: CAI45953.1
AL359513 Genomic DNA No translation available.
CH471274 Genomic DNA Translation: EAW55895.1
BC037279 mRNA Translation: AAH37279.1
BC050439 mRNA Translation: AAH50439.1
CCDSiCCDS73577.1 [Q86VN1-2]
CCDS9434.1 [Q86VN1-1]
RefSeqiNP_001269098.1, NM_001282169.1 [Q86VN1-2]
NP_057159.2, NM_016075.3 [Q86VN1-1]
UniGeneiHs.109520

Genome annotation databases

EnsembliENST00000378060; ENSP00000367299; ENSG00000136100 [Q86VN1-1]
ENST00000611132; ENSP00000484968; ENSG00000136100 [Q86VN1-2]
GeneIDi51028
KEGGihsa:51028
UCSCiuc001vgq.4 human [Q86VN1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151903 mRNA Translation: AAD34140.1
AK023182 mRNA Translation: BAB14451.1 Different initiation.
AK289740 mRNA Translation: BAF82429.1
CR933653 mRNA Translation: CAI45953.1
AL359513 Genomic DNA No translation available.
CH471274 Genomic DNA Translation: EAW55895.1
BC037279 mRNA Translation: AAH37279.1
BC050439 mRNA Translation: AAH50439.1
CCDSiCCDS73577.1 [Q86VN1-2]
CCDS9434.1 [Q86VN1-1]
RefSeqiNP_001269098.1, NM_001282169.1 [Q86VN1-2]
NP_057159.2, NM_016075.3 [Q86VN1-1]
UniGeneiHs.109520

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HTHX-ray2.70B1-138[»]
2ZMEX-ray2.90B149-386[»]
3CUQX-ray2.61B169-386[»]
ProteinModelPortaliQ86VN1
SMRiQ86VN1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119234, 43 interactors
CORUMiQ86VN1
DIPiDIP-29249N
IntActiQ86VN1, 6 interactors
MINTiQ86VN1
STRINGi9606.ENSP00000367299

PTM databases

iPTMnetiQ86VN1
PhosphoSitePlusiQ86VN1

Polymorphism and mutation databases

DMDMi73920464

Proteomic databases

EPDiQ86VN1
MaxQBiQ86VN1
PaxDbiQ86VN1
PeptideAtlasiQ86VN1
PRIDEiQ86VN1
ProteomicsDBi70043
70044 [Q86VN1-2]

Protocols and materials databases

DNASUi51028
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378060; ENSP00000367299; ENSG00000136100 [Q86VN1-1]
ENST00000611132; ENSP00000484968; ENSG00000136100 [Q86VN1-2]
GeneIDi51028
KEGGihsa:51028
UCSCiuc001vgq.4 human [Q86VN1-1]

Organism-specific databases

CTDi51028
DisGeNETi51028
EuPathDBiHostDB:ENSG00000136100.12
GeneCardsiVPS36
HGNCiHGNC:20312 VPS36
HPAiHPA039734
HPA043947
MIMi610903 gene
neXtProtiNX_Q86VN1
OpenTargetsiENSG00000136100
PharmGKBiPA134990943
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2760 Eukaryota
ENOG410XR74 LUCA
GeneTreeiENSGT00390000017209
HOVERGENiHBG083632
InParanoidiQ86VN1
KOiK12190
OMAiRKTRIIM
OrthoDBiEOG091G09HR
PhylomeDBiQ86VN1
TreeFamiTF314770

Enzyme and pathway databases

ReactomeiR-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSiVPS36 human
EvolutionaryTraceiQ86VN1
GeneWikiiVPS36
GenomeRNAii51028
PROiPR:Q86VN1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136100 Expressed in 200 organ(s), highest expression level in upper arm skin
CleanExiHS_VPS36
GenevisibleiQ86VN1 HS

Family and domain databases

Gene3Di1.10.10.10, 2 hits
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR021648 GLUE_dom
IPR011993 PH-like_dom_sf
IPR037855 Vps36
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13128 PTHR13128, 1 hit
PfamiView protein in Pfam
PF11605 Vps36_ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
PROSITEiView protein in PROSITE
PS51495 GLUE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPS36_HUMAN
AccessioniPrimary (citable) accession number: Q86VN1
Secondary accession number(s): A8K125
, Q3ZCV7, Q5H9S1, Q5VXB6, Q9H8Z5, Q9Y3E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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