UniProtKB - Q86VN1 (VPS36_HUMAN)
Vacuolar protein-sorting-associated protein 36
VPS36
Functioni
GO - Molecular functioni
- phosphatidylinositol-3-phosphate binding Source: InterPro
- protein C-terminus binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
GO - Biological processi
- endosomal transport Source: Reactome
- macroautophagy Source: ParkinsonsUK-UCL
- multivesicular body assembly Source: ParkinsonsUK-UCL
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
Keywordsi
Biological process | Protein transport, Transcription, Transcription regulation, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q86VN1 |
Reactomei | R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein-sorting-associated protein 36Alternative name(s): ELL-associated protein of 45 kDa ESCRT-II complex subunit VPS36 |
Gene namesi | Name:VPS36 Synonyms:C13orf9, EAP45 ORF Names:CGI-145 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20312, VPS36 |
MIMi | 610903, gene |
neXtProti | NX_Q86VN1 |
VEuPathDBi | HostDB:ENSG00000136100.12 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
- Nucleus Curated
Endosome
- Endosome
- Late endosome By similarity
Other locations
Note: Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.By similarity
Cytosol
- cytosol Source: UniProtKB
Endosome
- endosome Source: UniProtKB
- ESCRT II complex Source: UniProtKB
- late endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Endosome, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | L → D: No effect on interaction with ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 67 | V → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication | 1 | |
Mutagenesisi | 68 | F → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication | 1 | |
Mutagenesisi | 70 | E → A: Reduces affinity for ubiquitin up to 10-fold. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51028 |
OpenTargetsi | ENSG00000136100 |
PharmGKBi | PA134990943 |
Miscellaneous databases
Pharosi | Q86VN1, Tbio |
Genetic variation databases
BioMutai | VPS36 |
DMDMi | 73920464 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215222 | 1 – 386 | Vacuolar protein-sorting-associated protein 36Add BLAST | 386 |
Proteomic databases
EPDi | Q86VN1 |
jPOSTi | Q86VN1 |
MassIVEi | Q86VN1 |
MaxQBi | Q86VN1 |
PaxDbi | Q86VN1 |
PeptideAtlasi | Q86VN1 |
PRIDEi | Q86VN1 |
ProteomicsDBi | 70043 [Q86VN1-1] 70044 [Q86VN1-2] |
PTM databases
iPTMneti | Q86VN1 |
MetOSitei | Q86VN1 |
PhosphoSitePlusi | Q86VN1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000136100, Expressed in zone of skin and 217 other tissues |
ExpressionAtlasi | Q86VN1, baseline and differential |
Genevisiblei | Q86VN1, HS |
Organism-specific databases
HPAi | ENSG00000136100, Low tissue specificity |
Interactioni
Subunit structurei
Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity).
Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844).
Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118).
Interacts (via GLUE domain) with ubiquitin (PubMed:17057716).
Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938).
Interacts with ECPAS (PubMed:20682791).
By similarity7 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- protein C-terminus binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119234, 49 interactors |
CORUMi | Q86VN1 |
DIPi | DIP-29249N |
IntActi | Q86VN1, 11 interactors |
MINTi | Q86VN1 |
STRINGi | 9606.ENSP00000367299 |
Miscellaneous databases
RNActi | Q86VN1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q86VN1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q86VN1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 88 | GLUE N-terminalPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 105 – 138 | GLUE C-terminalPROSITE-ProRule annotationAdd BLAST | 34 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 160 – 185 | Sequence analysisAdd BLAST | 26 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2760, Eukaryota |
GeneTreei | ENSGT00390000017209 |
HOGENOMi | CLU_015433_0_0_1 |
InParanoidi | Q86VN1 |
OMAi | RKTRIIM |
OrthoDBi | 981902at2759 |
PhylomeDBi | Q86VN1 |
TreeFami | TF314770 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits 2.30.29.30, 1 hit |
IDEALi | IID00218 |
InterProi | View protein in InterPro IPR021648, GLUE_dom IPR011993, PH-like_dom_sf IPR040608, Snf8/Vps36 IPR037855, Vps36 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13128, PTHR13128, 1 hit |
Pfami | View protein in Pfam PF04157, EAP30, 1 hit PF11605, Vps36_ESCRT-II, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
PROSITEi | View protein in PROSITE PS51495, GLUE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDRFVWTSGL LEINETLVIQ QRGVRIYDGE EKIKFDAGTL LLSTHRLIWR
60 70 80 90 100
DQKNHECCMA ILLSQIVFIE EQAAGIGKSA KIVVHLHPAP PNKEPGPFQS
110 120 130 140 150
SKNSYIKLSF KEHGQIEFYR RLSEEMTQRR WENMPVSQSL QTNRGPQPGR
160 170 180 190 200
IRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN
210 220 230 240 250
KIKDKQGDIT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA
260 270 280 290 300
GILQVPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP
310 320 330 340 350
LRLRVFDSGV MVIELQSHKE EEMVASALET VSEKGSLTSE EFAKLVGMSV
360 370 380
LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3B3ISW7 | A0A3B3ISW7_HUMAN | Vacuolar protein-sorting-associated... | VPS36 | 324 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 185 | M → V in CAI45953 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 223 | S → R in AAD34140 (PubMed:10810093).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015342 | 1 – 58 | Missing in isoform 2. 1 PublicationAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF151903 mRNA Translation: AAD34140.1 AK023182 mRNA Translation: BAB14451.1 Different initiation. AK289740 mRNA Translation: BAF82429.1 CR933653 mRNA Translation: CAI45953.1 AL359513 Genomic DNA No translation available. CH471274 Genomic DNA Translation: EAW55895.1 BC037279 mRNA Translation: AAH37279.1 BC050439 mRNA Translation: AAH50439.1 |
CCDSi | CCDS73577.1 [Q86VN1-2] CCDS9434.1 [Q86VN1-1] |
RefSeqi | NP_001269098.1, NM_001282169.1 [Q86VN1-2] NP_057159.2, NM_016075.3 [Q86VN1-1] |
Genome annotation databases
Ensembli | ENST00000378060; ENSP00000367299; ENSG00000136100 [Q86VN1-1] ENST00000611132; ENSP00000484968; ENSG00000136100 [Q86VN1-2] |
GeneIDi | 51028 |
KEGGi | hsa:51028 |
UCSCi | uc001vgq.4, human [Q86VN1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF151903 mRNA Translation: AAD34140.1 AK023182 mRNA Translation: BAB14451.1 Different initiation. AK289740 mRNA Translation: BAF82429.1 CR933653 mRNA Translation: CAI45953.1 AL359513 Genomic DNA No translation available. CH471274 Genomic DNA Translation: EAW55895.1 BC037279 mRNA Translation: AAH37279.1 BC050439 mRNA Translation: AAH50439.1 |
CCDSi | CCDS73577.1 [Q86VN1-2] CCDS9434.1 [Q86VN1-1] |
RefSeqi | NP_001269098.1, NM_001282169.1 [Q86VN1-2] NP_057159.2, NM_016075.3 [Q86VN1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HTH | X-ray | 2.70 | B | 1-138 | [»] | |
2ZME | X-ray | 2.90 | B | 149-386 | [»] | |
3CUQ | X-ray | 2.61 | B | 169-386 | [»] | |
SMRi | Q86VN1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119234, 49 interactors |
CORUMi | Q86VN1 |
DIPi | DIP-29249N |
IntActi | Q86VN1, 11 interactors |
MINTi | Q86VN1 |
STRINGi | 9606.ENSP00000367299 |
PTM databases
iPTMneti | Q86VN1 |
MetOSitei | Q86VN1 |
PhosphoSitePlusi | Q86VN1 |
Genetic variation databases
BioMutai | VPS36 |
DMDMi | 73920464 |
Proteomic databases
EPDi | Q86VN1 |
jPOSTi | Q86VN1 |
MassIVEi | Q86VN1 |
MaxQBi | Q86VN1 |
PaxDbi | Q86VN1 |
PeptideAtlasi | Q86VN1 |
PRIDEi | Q86VN1 |
ProteomicsDBi | 70043 [Q86VN1-1] 70044 [Q86VN1-2] |
Protocols and materials databases
Antibodypediai | 24193, 60 antibodies |
DNASUi | 51028 |
Genome annotation databases
Ensembli | ENST00000378060; ENSP00000367299; ENSG00000136100 [Q86VN1-1] ENST00000611132; ENSP00000484968; ENSG00000136100 [Q86VN1-2] |
GeneIDi | 51028 |
KEGGi | hsa:51028 |
UCSCi | uc001vgq.4, human [Q86VN1-1] |
Organism-specific databases
CTDi | 51028 |
DisGeNETi | 51028 |
GeneCardsi | VPS36 |
HGNCi | HGNC:20312, VPS36 |
HPAi | ENSG00000136100, Low tissue specificity |
MIMi | 610903, gene |
neXtProti | NX_Q86VN1 |
OpenTargetsi | ENSG00000136100 |
PharmGKBi | PA134990943 |
VEuPathDBi | HostDB:ENSG00000136100.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2760, Eukaryota |
GeneTreei | ENSGT00390000017209 |
HOGENOMi | CLU_015433_0_0_1 |
InParanoidi | Q86VN1 |
OMAi | RKTRIIM |
OrthoDBi | 981902at2759 |
PhylomeDBi | Q86VN1 |
TreeFami | TF314770 |
Enzyme and pathway databases
PathwayCommonsi | Q86VN1 |
Reactomei | R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events |
Miscellaneous databases
BioGRID-ORCSi | 51028, 76 hits in 1011 CRISPR screens |
ChiTaRSi | VPS36, human |
EvolutionaryTracei | Q86VN1 |
GeneWikii | VPS36 |
GenomeRNAii | 51028 |
Pharosi | Q86VN1, Tbio |
PROi | PR:Q86VN1 |
RNActi | Q86VN1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000136100, Expressed in zone of skin and 217 other tissues |
ExpressionAtlasi | Q86VN1, baseline and differential |
Genevisiblei | Q86VN1, HS |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits 2.30.29.30, 1 hit |
IDEALi | IID00218 |
InterProi | View protein in InterPro IPR021648, GLUE_dom IPR011993, PH-like_dom_sf IPR040608, Snf8/Vps36 IPR037855, Vps36 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13128, PTHR13128, 1 hit |
Pfami | View protein in Pfam PF04157, EAP30, 1 hit PF11605, Vps36_ESCRT-II, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
PROSITEi | View protein in PROSITE PS51495, GLUE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS36_HUMAN | |
Accessioni | Q86VN1Primary (citable) accession number: Q86VN1 Secondary accession number(s): A8K125 Q9Y3E3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families