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UniProtKB - Q86VL8 (S47A2_HUMAN)

Protein

Multidrug and toxin extrusion protein 2

Gene

SLC47A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, and ganciclovir. Responsible for the secretion of cationic drugs across the brush border membranes.3 Publications

Kineticsi

  1. KM=0.76 mM for TEA2 Publications
  2. KM=0.11 mM for MPP2 Publications
  3. KM=0.12 mM for cimetidine2 Publications
  4. KM=1.98 mM for metformin2 Publications
  5. KM=4.2 mM for guanidine2 Publications
  6. KM=1.58 mM for procainamide2 Publications
  7. KM=0.06 mM for topotecan2 Publications
  8. KM=0.85 mM for estrone sulfate2 Publications
  9. KM=4.32 mM for acyclovir2 Publications
  10. KM=4.28 mM for ganciclovir2 Publications
  1. Vmax=0.88 nmol/min/mg enzyme toward TEA2 Publications
  2. Vmax=0.575 nmol/min/mg enzyme toward MPP2 Publications
  3. Vmax=0.115 nmol/min/mg enzyme toward cimetidine2 Publications
  4. Vmax=0.845 nmol/min/mg enzyme toward metformin2 Publications
  5. Vmax=0.58 nmol/min/mg enzyme toward guanidine2 Publications
  6. Vmax=3.385 nmol/min/mg enzyme toward procainamide2 Publications
  7. Vmax=0.13 nmol/min/mg enzyme toward topotecan2 Publications
  8. Vmax=0.425 nmol/min/mg enzyme toward estrone sulfate2 Publications
  9. Vmax=0.945 nmol/min/mg enzyme toward acyclovir2 Publications
  10. Vmax=0.805 nmol/min/mg enzyme toward ganciclovir2 Publications

pH dependencei

Optimum pH is 9.0. Active from pH 6 to 9.2 Publications

GO - Molecular functioni

  • drug:proton antiporter activity Source: Reactome
  • drug transmembrane transporter activity Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-HSA-425366 Transport of bile salts and organic acids, metal ions and amine compounds

Protein family/group databases

TCDBi2.A.66.1.20 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 2
Short name:
MATE-2
Short name:
hMATE-2
Alternative name(s):
Kidney-specific H(+)/organic cation antiporter
Solute carrier family 47 member 2
Gene namesi
Name:SLC47A2
Synonyms:MATE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000180638.17
HGNCiHGNC:26439 SLC47A2
MIMi609833 gene
neXtProtiNX_Q86VL8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 66ExtracellularSequence analysisAdd BLAST12
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 119CytoplasmicSequence analysisAdd BLAST32
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 153ExtracellularSequence analysisAdd BLAST13
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Topological domaini175 – 219CytoplasmicSequence analysisAdd BLAST45
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 248ExtracellularSequence analysis8
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 289CytoplasmicSequence analysisAdd BLAST20
Transmembranei290 – 309HelicalSequence analysisAdd BLAST20
Topological domaini310 – 327ExtracellularSequence analysisAdd BLAST18
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 368CytoplasmicSequence analysisAdd BLAST20
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Topological domaini390 – 402ExtracellularSequence analysisAdd BLAST13
Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
Topological domaini424 – 442CytoplasmicSequence analysisAdd BLAST19
Transmembranei443 – 463HelicalSequence analysisAdd BLAST21
Topological domaini464 – 466ExtracellularSequence analysis3
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Topological domaini488 – 578CytoplasmicSequence analysisAdd BLAST91
Transmembranei579 – 599HelicalSequence analysisAdd BLAST21
Topological domaini600 – 602ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi146802
OpenTargetsiENSG00000180638
PharmGKBiPA162403847

Chemistry databases

ChEMBLiCHEMBL1743127
DrugBankiDB00787 Aciclovir
GuidetoPHARMACOLOGYi1217

Polymorphism and mutation databases

BioMutaiSLC47A2
DMDMi74727585

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003119521 – 602Multidrug and toxin extrusion protein 2Add BLAST602

Proteomic databases

PaxDbiQ86VL8
PeptideAtlasiQ86VL8
PRIDEiQ86VL8
ProteomicsDBi70034
70035 [Q86VL8-2]
70036 [Q86VL8-3]
70037 [Q86VL8-4]
70038 [Q86VL8-5]
70039 [Q86VL8-6]

PTM databases

iPTMnetiQ86VL8
PhosphoSitePlusiQ86VL8

Expressioni

Tissue specificityi

Isoform 3 is predominantly expressed in kidney. Isoform 6 is expressed in brain.1 Publication

Gene expression databases

BgeeiENSG00000180638 Expressed in 99 organ(s), highest expression level in kidney epithelium
CleanExiHS_SLC47A2
ExpressionAtlasiQ86VL8 baseline and differential
GenevisibleiQ86VL8 HS

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000326671

Chemistry databases

BindingDBiQ86VL8

Structurei

3D structure databases

ProteinModelPortaliQ86VL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi508 – 511Poly-Gln4

Sequence similaritiesi

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347 Eukaryota
COG0534 LUCA
GeneTreeiENSGT00390000015713
HOGENOMiHOG000060313
HOVERGENiHBG056043
InParanoidiQ86VL8
KOiK03327
OMAiRMVSGRW
OrthoDBiEOG091G06X2
PhylomeDBiQ86VL8
TreeFamiTF324441

Family and domain databases

InterProiView protein in InterPro
IPR002528 MATE_fam
PfamiView protein in Pfam
PF01554 MatE, 2 hits
TIGRFAMsiTIGR00797 matE, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86VL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSLQDTVAL DHGGCCPALS RLVPRGFGTE MWTLFALSGP LFLFQVLTFM 
        60         70         80         90        100
IYIVSTVFCG HLGKVELASV TLAVAFVNVC GVSVGVGLSS ACDTLMSQSF 
       110        120        130        140        150
GSPNKKHVGV ILQRGALVLL LCCLPCWALF LNTQHILLLF RQDPDVSRLT 
       160        170        180        190        200
QDYVMIFIPG LPVIFLYNLL AKYLQNQGWL KGQEEESPFQ TPGLSILHPS 
       210        220        230        240        250
HSHLSRASFH LFQKITWPQV LSGVVGNCVN GVANYALVSV LNLGVRGSAY 
       260        270        280        290        300
ANIISQFAQT VFLLLYIVLK KLHLETWAGW SSQCLQDWGP FFSLAVPSML 
       310        320        330        340        350
MICVEWWAYE IGSFLMGLLS VVDLSAQAVI YEVATVTYMI PLGLSIGVCV 
       360        370        380        390        400
RVGMALGAAD TVQAKRSAVS GVLSIVGISL VLGTLISILK NQLGHIFTND 
       410        420        430        440        450
EDVIALVSQV LPVYSVFHVF EAICCVYGGV LRGTGKQAFG AAVNAITYYI 
       460        470        480        490        500
IGLPLGILLT FVVRMRIMGL WLGMLACVFL ATAAFVAYTA RLDWKLAAEE 
       510        520        530        540        550
AKKHSGRQQQ QRAESTATRP GPEKAVLSSV ATGSSPGITL TTYSRSECHV 
       560        570        580        590        600
DFFRTPEEAH ALSAPTSRLS VKQLVIRRGA ALGAASATLM VGLTVRILAT 

RH
Length:602
Mass (Da):65,085
Last modified:June 1, 2003 - v1
Checksum:i88EB90286AE701D1
GO
Isoform 2 (identifier: Q86VL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     178-213: Missing.
     376-381: VGISLV → EMSLPW
     382-602: Missing.

Note: No experimental confirmation available.
Show »
Length:296
Mass (Da):32,120
Checksum:iA8233F83A55432EC
GO
Isoform 3 (identifier: Q86VL8-3) [UniParc]FASTAAdd to basket
Also known as: MATE2-K

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.

Note: No experimental confirmation available.
Show »
Length:566
Mass (Da):61,016
Checksum:iA3D0B0E578E0B8C2
GO
Isoform 4 (identifier: Q86VL8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.
     339-339: M → MRHSHRLAYAAHVTR

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):62,673
Checksum:i848F216F93C7F1C7
GO
Isoform 5 (identifier: Q86VL8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.
     317-398: GLLSVVDLSA...LKNQLGHIFT → EHAQCGGSLC...EKSAGAYFYQ
     399-602: Missing.

Note: No experimental confirmation available.
Show »
Length:362
Mass (Da):39,287
Checksum:i35A2CE7A78E3E19D
GO
Isoform 6 (identifier: Q86VL8-6) [UniParc]FASTAAdd to basket
Also known as: MATE2-B

The sequence of this isoform differs from the canonical sequence as follows:
     178-219: GWLKGQEEES...HLFQKITWPQ → AGERLCVPAS...VHPPSISLTP
     220-602: Missing.

Note: Inactive. No experimental confirmation available.
Show »
Length:219
Mass (Da):23,344
Checksum:i40D6F1AA297DAA91
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JAE6C9JAE6_HUMAN
Multidrug and toxin extrusion prote...
SLC47A2
363Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6D → E in BAF37007 (PubMed:16807400).Curated1
Sequence conflicti23V → A in AK055758 (PubMed:14702039).Curated1
Sequence conflicti93D → G in AK055758 (PubMed:14702039).Curated1
Sequence conflicti284C → Y in CAH56154 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037358429G → R. Corresponds to variant dbSNP:rs34399035Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0296561 – 49Missing in isoform 2. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_029657178 – 219GWLKG…ITWPQ → AGERLCVPASSNSSTGMAEG AGGGVPIPNPGFVHPPSISL TP in isoform 6. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_029658178 – 213Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST36
Alternative sequenceiVSP_029659220 – 602Missing in isoform 6. 1 PublicationAdd BLAST383
Alternative sequenceiVSP_029660317 – 398GLLSV…GHIFT → EHAQCGGSLCPGCHLGGHCD LHDSLGAQHRGLCPSGDGSG GRGYCAGQALGRLGRAQHSW HFPGPGHPDKHPEKSAGAYF YQ in isoform 5. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_029662339M → MRHSHRLAYAAHVTR in isoform 4. 1 Publication1
Alternative sequenceiVSP_029663376 – 381VGISLV → EMSLPW in isoform 2. 1 Publication6
Alternative sequenceiVSP_029664382 – 602Missing in isoform 2. 1 PublicationAdd BLAST221
Alternative sequenceiVSP_029661399 – 602Missing in isoform 5. 1 PublicationAdd BLAST204

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250364 mRNA Translation: BAF36847.1
AB250701 mRNA Translation: BAF37007.1
AK055758 mRNA No translation available.
BX648861 mRNA Translation: CAH56154.1
CH471212 Genomic DNA Translation: EAW50902.1
CH471212 Genomic DNA Translation: EAW50904.1
CH471212 Genomic DNA Translation: EAW50905.1
BC035288 mRNA Translation: AAH35288.1
BC050578 mRNA Translation: AAH50578.1
CCDSiCCDS11211.1 [Q86VL8-1]
CCDS58530.1 [Q86VL8-4]
RefSeqiNP_001093116.1, NM_001099646.1 [Q86VL8-3]
NP_001243592.1, NM_001256663.1 [Q86VL8-4]
NP_690872.2, NM_152908.3 [Q86VL8-1]
UniGeneiHs.126830

Genome annotation databases

EnsembliENST00000325411; ENSP00000326671; ENSG00000180638 [Q86VL8-1]
ENST00000350657; ENSP00000338084; ENSG00000180638 [Q86VL8-4]
ENST00000574239; ENSP00000458694; ENSG00000180638 [Q86VL8-6]
GeneIDi146802
KEGGihsa:146802
UCSCiuc002gwe.5 human [Q86VL8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250364 mRNA Translation: BAF36847.1
AB250701 mRNA Translation: BAF37007.1
AK055758 mRNA No translation available.
BX648861 mRNA Translation: CAH56154.1
CH471212 Genomic DNA Translation: EAW50902.1
CH471212 Genomic DNA Translation: EAW50904.1
CH471212 Genomic DNA Translation: EAW50905.1
BC035288 mRNA Translation: AAH35288.1
BC050578 mRNA Translation: AAH50578.1
CCDSiCCDS11211.1 [Q86VL8-1]
CCDS58530.1 [Q86VL8-4]
RefSeqiNP_001093116.1, NM_001099646.1 [Q86VL8-3]
NP_001243592.1, NM_001256663.1 [Q86VL8-4]
NP_690872.2, NM_152908.3 [Q86VL8-1]
UniGeneiHs.126830

3D structure databases

ProteinModelPortaliQ86VL8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000326671

Chemistry databases

BindingDBiQ86VL8
ChEMBLiCHEMBL1743127
DrugBankiDB00787 Aciclovir
GuidetoPHARMACOLOGYi1217

Protein family/group databases

TCDBi2.A.66.1.20 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

PTM databases

iPTMnetiQ86VL8
PhosphoSitePlusiQ86VL8

Polymorphism and mutation databases

BioMutaiSLC47A2
DMDMi74727585

Proteomic databases

PaxDbiQ86VL8
PeptideAtlasiQ86VL8
PRIDEiQ86VL8
ProteomicsDBi70034
70035 [Q86VL8-2]
70036 [Q86VL8-3]
70037 [Q86VL8-4]
70038 [Q86VL8-5]
70039 [Q86VL8-6]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325411; ENSP00000326671; ENSG00000180638 [Q86VL8-1]
ENST00000350657; ENSP00000338084; ENSG00000180638 [Q86VL8-4]
ENST00000574239; ENSP00000458694; ENSG00000180638 [Q86VL8-6]
GeneIDi146802
KEGGihsa:146802
UCSCiuc002gwe.5 human [Q86VL8-1]

Organism-specific databases

CTDi146802
DisGeNETi146802
EuPathDBiHostDB:ENSG00000180638.17
GeneCardsiSLC47A2
H-InvDBiHIX0039367
HGNCiHGNC:26439 SLC47A2
MIMi609833 gene
neXtProtiNX_Q86VL8
OpenTargetsiENSG00000180638
PharmGKBiPA162403847
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1347 Eukaryota
COG0534 LUCA
GeneTreeiENSGT00390000015713
HOGENOMiHOG000060313
HOVERGENiHBG056043
InParanoidiQ86VL8
KOiK03327
OMAiRMVSGRW
OrthoDBiEOG091G06X2
PhylomeDBiQ86VL8
TreeFamiTF324441

Enzyme and pathway databases

ReactomeiR-HSA-425366 Transport of bile salts and organic acids, metal ions and amine compounds

Miscellaneous databases

GeneWikiiSLC47A2
GenomeRNAii146802
PROiPR:Q86VL8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180638 Expressed in 99 organ(s), highest expression level in kidney epithelium
CleanExiHS_SLC47A2
ExpressionAtlasiQ86VL8 baseline and differential
GenevisibleiQ86VL8 HS

Family and domain databases

InterProiView protein in InterPro
IPR002528 MATE_fam
PfamiView protein in Pfam
PF01554 MatE, 2 hits
TIGRFAMsiTIGR00797 matE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiS47A2_HUMAN
AccessioniPrimary (citable) accession number: Q86VL8
Secondary accession number(s): A0JBX9
, A0P8Z7, Q63HJ9, Q8IV44, Q96NA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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