UniProtKB - Q86VL8 (S47A2_HUMAN)
Protein
Multidrug and toxin extrusion protein 2
Gene
SLC47A2
Organism
Homo sapiens (Human)
Status
Functioni
Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, and ganciclovir. Responsible for the secretion of cationic drugs across the brush border membranes.3 Publications
Kineticsi
- KM=0.76 mM for TEA2 Publications
- KM=0.11 mM for MPP2 Publications
- KM=0.12 mM for cimetidine2 Publications
- KM=1.98 mM for metformin2 Publications
- KM=4.2 mM for guanidine2 Publications
- KM=1.58 mM for procainamide2 Publications
- KM=0.06 mM for topotecan2 Publications
- KM=0.85 mM for estrone sulfate2 Publications
- KM=4.32 mM for acyclovir2 Publications
- KM=4.28 mM for ganciclovir2 Publications
- Vmax=0.88 nmol/min/mg enzyme toward TEA2 Publications
- Vmax=0.575 nmol/min/mg enzyme toward MPP2 Publications
- Vmax=0.115 nmol/min/mg enzyme toward cimetidine2 Publications
- Vmax=0.845 nmol/min/mg enzyme toward metformin2 Publications
- Vmax=0.58 nmol/min/mg enzyme toward guanidine2 Publications
- Vmax=3.385 nmol/min/mg enzyme toward procainamide2 Publications
- Vmax=0.13 nmol/min/mg enzyme toward topotecan2 Publications
- Vmax=0.425 nmol/min/mg enzyme toward estrone sulfate2 Publications
- Vmax=0.945 nmol/min/mg enzyme toward acyclovir2 Publications
- Vmax=0.805 nmol/min/mg enzyme toward ganciclovir2 Publications
pH dependencei
Optimum pH is 9.0. Active from pH 6 to 9.2 Publications
GO - Molecular functioni
- antiporter activity Source: InterPro
- transmembrane transporter activity Source: GO_Central
- xenobiotic transmembrane transporter activity Source: InterPro
GO - Biological processi
- transmembrane transport Source: Reactome
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | Q86VL8 |
Reactomei | R-HSA-425366, Transport of bile salts and organic acids, metal ions and amine compounds |
Protein family/group databases
TCDBi | 2.A.66.1.20, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug and toxin extrusion protein 2Short name: MATE-2 Short name: hMATE-2 Alternative name(s): Kidney-specific H(+)/organic cation antiporter Solute carrier family 47 member 2 |
Gene namesi | Name:SLC47A2 Synonyms:MATE2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26439, SLC47A2 |
MIMi | 609833, gene |
neXtProti | NX_Q86VL8 |
VEuPathDBi | HostDB:ENSG00000180638.17 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Note: Localized at the brush border membranes of the proximal tubules.
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 66 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 67 – 87 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 88 – 119 | CytoplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 120 – 140 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 141 – 153 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 154 – 174 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 175 – 219 | CytoplasmicSequence analysisAdd BLAST | 45 | |
Transmembranei | 220 – 240 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 241 – 248 | ExtracellularSequence analysis | 8 | |
Transmembranei | 249 – 269 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 270 – 289 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 290 – 309 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 310 – 327 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 328 – 348 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 349 – 368 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 369 – 389 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 390 – 402 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 403 – 423 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 424 – 442 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 443 – 463 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 464 – 466 | ExtracellularSequence analysis | 3 | |
Transmembranei | 467 – 487 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 488 – 578 | CytoplasmicSequence analysisAdd BLAST | 91 | |
Transmembranei | 579 – 599 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 600 – 602 | ExtracellularSequence analysis | 3 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 146802 |
OpenTargetsi | ENSG00000180638 |
PharmGKBi | PA162403847 |
Miscellaneous databases
Pharosi | Q86VL8, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1743127 |
DrugBanki | DB12001, Abemaciclib DB00787, Acyclovir DB11901, Apalutamide DB11817, Baricitinib DB12267, Brigatinib DB11791, Capmatinib DB00501, Cimetidine DB00537, Ciprofloxacin DB12483, Copanlisib DB04574, Estrone sulfate DB12500, Fedratinib DB01004, Ganciclovir DB11978, Glasdegib DB00536, Guanidine DB11757, Istradefylline DB11732, Lasmiditan DB01137, Levofloxacin DB00331, Metformin DB09241, Methylene blue DB08840, N-methylnicotinamide DB01203, Nadolol DB12978, Pexidartinib DB12615, Plazomicin DB15822, Pralsetinib DB01035, Procainamide DB00205, Pyrimethamine DB00243, Ranolazine DB12377, Relebactam DB15305, Risdiplam DB12332, Rucaparib DB06608, Tafenoquine DB12887, Tazemetostat DB06137, Tirbanibulin DB01030, Topotecan DB14962, Trastuzumab deruxtecan DB00440, Trimethoprim DB11652, Tucatinib DB00661, Verapamil |
DrugCentrali | Q86VL8 |
GuidetoPHARMACOLOGYi | 1217 |
Genetic variation databases
BioMutai | SLC47A2 |
DMDMi | 74727585 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000311952 | 1 – 602 | Multidrug and toxin extrusion protein 2Add BLAST | 602 |
Proteomic databases
MassIVEi | Q86VL8 |
PaxDbi | Q86VL8 |
PeptideAtlasi | Q86VL8 |
PRIDEi | Q86VL8 |
ProteomicsDBi | 70034 [Q86VL8-1] 70035 [Q86VL8-2] 70036 [Q86VL8-3] 70037 [Q86VL8-4] 70038 [Q86VL8-5] 70039 [Q86VL8-6] |
PTM databases
iPTMneti | Q86VL8 |
PhosphoSitePlusi | Q86VL8 |
Expressioni
Tissue specificityi
Isoform 3 is predominantly expressed in kidney. Isoform 6 is expressed in brain.1 Publication
Gene expression databases
Bgeei | ENSG00000180638, Expressed in kidney epithelium and 119 other tissues |
ExpressionAtlasi | Q86VL8, baseline and differential |
Genevisiblei | Q86VL8, HS |
Organism-specific databases
HPAi | ENSG00000180638, Tissue enriched (kidney) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 127012, 3 interactors |
IntActi | Q86VL8, 2 interactors |
STRINGi | 9606.ENSP00000326671 |
Chemistry databases
BindingDBi | Q86VL8 |
Miscellaneous databases
RNActi | Q86VL8, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 508 – 511 | Poly-Gln | 4 |
Sequence similaritiesi
Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1347, Eukaryota |
GeneTreei | ENSGT00940000163062 |
HOGENOMi | CLU_012893_1_3_1 |
InParanoidi | Q86VL8 |
OMAi | CQGIFHA |
OrthoDBi | 743037at2759 |
PhylomeDBi | Q86VL8 |
TreeFami | TF324441 |
Family and domain databases
InterProi | View protein in InterPro IPR002528, MATE_fam |
Pfami | View protein in Pfam PF01554, MatE, 2 hits |
TIGRFAMsi | TIGR00797, matE, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q86VL8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSLQDTVAL DHGGCCPALS RLVPRGFGTE MWTLFALSGP LFLFQVLTFM
60 70 80 90 100
IYIVSTVFCG HLGKVELASV TLAVAFVNVC GVSVGVGLSS ACDTLMSQSF
110 120 130 140 150
GSPNKKHVGV ILQRGALVLL LCCLPCWALF LNTQHILLLF RQDPDVSRLT
160 170 180 190 200
QDYVMIFIPG LPVIFLYNLL AKYLQNQGWL KGQEEESPFQ TPGLSILHPS
210 220 230 240 250
HSHLSRASFH LFQKITWPQV LSGVVGNCVN GVANYALVSV LNLGVRGSAY
260 270 280 290 300
ANIISQFAQT VFLLLYIVLK KLHLETWAGW SSQCLQDWGP FFSLAVPSML
310 320 330 340 350
MICVEWWAYE IGSFLMGLLS VVDLSAQAVI YEVATVTYMI PLGLSIGVCV
360 370 380 390 400
RVGMALGAAD TVQAKRSAVS GVLSIVGISL VLGTLISILK NQLGHIFTND
410 420 430 440 450
EDVIALVSQV LPVYSVFHVF EAICCVYGGV LRGTGKQAFG AAVNAITYYI
460 470 480 490 500
IGLPLGILLT FVVRMRIMGL WLGMLACVFL ATAAFVAYTA RLDWKLAAEE
510 520 530 540 550
AKKHSGRQQQ QRAESTATRP GPEKAVLSSV ATGSSPGITL TTYSRSECHV
560 570 580 590 600
DFFRTPEEAH ALSAPTSRLS VKQLVIRRGA ALGAASATLM VGLTVRILAT
RH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JAE6 | C9JAE6_HUMAN | Multidrug and toxin extrusion prote... | SLC47A2 | 363 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | D → E in BAF37007 (PubMed:16807400).Curated | 1 | |
Sequence conflicti | 23 | V → A in AK055758 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 93 | D → G in AK055758 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 284 | C → Y in CAH56154 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037358 | 429 | G → R. Corresponds to variant dbSNP:rs34399035Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029656 | 1 – 49 | Missing in isoform 2. 1 PublicationAdd BLAST | 49 | |
Alternative sequenceiVSP_029657 | 178 – 219 | GWLKG…ITWPQ → AGERLCVPASSNSSTGMAEG AGGGVPIPNPGFVHPPSISL TP in isoform 6. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_029658 | 178 – 213 | Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST | 36 | |
Alternative sequenceiVSP_029659 | 220 – 602 | Missing in isoform 6. 1 PublicationAdd BLAST | 383 | |
Alternative sequenceiVSP_029660 | 317 – 398 | GLLSV…GHIFT → EHAQCGGSLCPGCHLGGHCD LHDSLGAQHRGLCPSGDGSG GRGYCAGQALGRLGRAQHSW HFPGPGHPDKHPEKSAGAYF YQ in isoform 5. 1 PublicationAdd BLAST | 82 | |
Alternative sequenceiVSP_029662 | 339 | M → MRHSHRLAYAAHVTR in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_029663 | 376 – 381 | VGISLV → EMSLPW in isoform 2. 1 Publication | 6 | |
Alternative sequenceiVSP_029664 | 382 – 602 | Missing in isoform 2. 1 PublicationAdd BLAST | 221 | |
Alternative sequenceiVSP_029661 | 399 – 602 | Missing in isoform 5. 1 PublicationAdd BLAST | 204 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB250364 mRNA Translation: BAF36847.1 AB250701 mRNA Translation: BAF37007.1 AK055758 mRNA No translation available. BX648861 mRNA Translation: CAH56154.1 CH471212 Genomic DNA Translation: EAW50902.1 CH471212 Genomic DNA Translation: EAW50904.1 CH471212 Genomic DNA Translation: EAW50905.1 BC035288 mRNA Translation: AAH35288.1 BC050578 mRNA Translation: AAH50578.1 |
CCDSi | CCDS11211.1 [Q86VL8-1] CCDS58530.1 [Q86VL8-4] |
RefSeqi | NP_001093116.1, NM_001099646.1 [Q86VL8-3] NP_001243592.1, NM_001256663.1 [Q86VL8-4] NP_690872.2, NM_152908.3 [Q86VL8-1] |
Genome annotation databases
Ensembli | ENST00000325411; ENSP00000326671; ENSG00000180638 [Q86VL8-1] ENST00000350657; ENSP00000338084; ENSG00000180638 [Q86VL8-4] ENST00000433844; ENSP00000391848; ENSG00000180638 [Q86VL8-3] ENST00000574239; ENSP00000458694; ENSG00000180638 [Q86VL8-6] |
GeneIDi | 146802 |
KEGGi | hsa:146802 |
UCSCi | uc002gwe.5, human [Q86VL8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB250364 mRNA Translation: BAF36847.1 AB250701 mRNA Translation: BAF37007.1 AK055758 mRNA No translation available. BX648861 mRNA Translation: CAH56154.1 CH471212 Genomic DNA Translation: EAW50902.1 CH471212 Genomic DNA Translation: EAW50904.1 CH471212 Genomic DNA Translation: EAW50905.1 BC035288 mRNA Translation: AAH35288.1 BC050578 mRNA Translation: AAH50578.1 |
CCDSi | CCDS11211.1 [Q86VL8-1] CCDS58530.1 [Q86VL8-4] |
RefSeqi | NP_001093116.1, NM_001099646.1 [Q86VL8-3] NP_001243592.1, NM_001256663.1 [Q86VL8-4] NP_690872.2, NM_152908.3 [Q86VL8-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 127012, 3 interactors |
IntActi | Q86VL8, 2 interactors |
STRINGi | 9606.ENSP00000326671 |
Chemistry databases
BindingDBi | Q86VL8 |
ChEMBLi | CHEMBL1743127 |
DrugBanki | DB12001, Abemaciclib DB00787, Acyclovir DB11901, Apalutamide DB11817, Baricitinib DB12267, Brigatinib DB11791, Capmatinib DB00501, Cimetidine DB00537, Ciprofloxacin DB12483, Copanlisib DB04574, Estrone sulfate DB12500, Fedratinib DB01004, Ganciclovir DB11978, Glasdegib DB00536, Guanidine DB11757, Istradefylline DB11732, Lasmiditan DB01137, Levofloxacin DB00331, Metformin DB09241, Methylene blue DB08840, N-methylnicotinamide DB01203, Nadolol DB12978, Pexidartinib DB12615, Plazomicin DB15822, Pralsetinib DB01035, Procainamide DB00205, Pyrimethamine DB00243, Ranolazine DB12377, Relebactam DB15305, Risdiplam DB12332, Rucaparib DB06608, Tafenoquine DB12887, Tazemetostat DB06137, Tirbanibulin DB01030, Topotecan DB14962, Trastuzumab deruxtecan DB00440, Trimethoprim DB11652, Tucatinib DB00661, Verapamil |
DrugCentrali | Q86VL8 |
GuidetoPHARMACOLOGYi | 1217 |
Protein family/group databases
TCDBi | 2.A.66.1.20, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
PTM databases
iPTMneti | Q86VL8 |
PhosphoSitePlusi | Q86VL8 |
Genetic variation databases
BioMutai | SLC47A2 |
DMDMi | 74727585 |
Proteomic databases
MassIVEi | Q86VL8 |
PaxDbi | Q86VL8 |
PeptideAtlasi | Q86VL8 |
PRIDEi | Q86VL8 |
ProteomicsDBi | 70034 [Q86VL8-1] 70035 [Q86VL8-2] 70036 [Q86VL8-3] 70037 [Q86VL8-4] 70038 [Q86VL8-5] 70039 [Q86VL8-6] |
Protocols and materials databases
Antibodypediai | 26005, 161 antibodies |
Genome annotation databases
Ensembli | ENST00000325411; ENSP00000326671; ENSG00000180638 [Q86VL8-1] ENST00000350657; ENSP00000338084; ENSG00000180638 [Q86VL8-4] ENST00000433844; ENSP00000391848; ENSG00000180638 [Q86VL8-3] ENST00000574239; ENSP00000458694; ENSG00000180638 [Q86VL8-6] |
GeneIDi | 146802 |
KEGGi | hsa:146802 |
UCSCi | uc002gwe.5, human [Q86VL8-1] |
Organism-specific databases
CTDi | 146802 |
DisGeNETi | 146802 |
GeneCardsi | SLC47A2 |
HGNCi | HGNC:26439, SLC47A2 |
HPAi | ENSG00000180638, Tissue enriched (kidney) |
MIMi | 609833, gene |
neXtProti | NX_Q86VL8 |
OpenTargetsi | ENSG00000180638 |
PharmGKBi | PA162403847 |
VEuPathDBi | HostDB:ENSG00000180638.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1347, Eukaryota |
GeneTreei | ENSGT00940000163062 |
HOGENOMi | CLU_012893_1_3_1 |
InParanoidi | Q86VL8 |
OMAi | CQGIFHA |
OrthoDBi | 743037at2759 |
PhylomeDBi | Q86VL8 |
TreeFami | TF324441 |
Enzyme and pathway databases
PathwayCommonsi | Q86VL8 |
Reactomei | R-HSA-425366, Transport of bile salts and organic acids, metal ions and amine compounds |
Miscellaneous databases
BioGRID-ORCSi | 146802, 7 hits in 979 CRISPR screens |
GeneWikii | SLC47A2 |
GenomeRNAii | 146802 |
Pharosi | Q86VL8, Tchem |
PROi | PR:Q86VL8 |
RNActi | Q86VL8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000180638, Expressed in kidney epithelium and 119 other tissues |
ExpressionAtlasi | Q86VL8, baseline and differential |
Genevisiblei | Q86VL8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002528, MATE_fam |
Pfami | View protein in Pfam PF01554, MatE, 2 hits |
TIGRFAMsi | TIGR00797, matE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S47A2_HUMAN | |
Accessioni | Q86VL8Primary (citable) accession number: Q86VL8 Secondary accession number(s): A0JBX9 Q96NA1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families