UniProtKB - Q86VF5 (MOGT3_HUMAN)
Protein
2-acylglycerol O-acyltransferase 3
Gene
MOGAT3
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Also able to catalyze the terminal step in triacylglycerol synthesis by using diacylglycerol and fatty acyl-CoA as substrates. Has a preference toward palmitoyl-CoA and oleoyl-CoA. May be involved in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).3 Publications
Catalytic activityi
- EC:2.3.1.222 PublicationsThis reaction proceeds in the forwardCurated direction.
- EC:2.3.1.202 PublicationsThis reaction proceeds in the forwardCurated direction.
- (9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-octadecenoyl)glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- Vmax=9.3 nmol/min/mg enzyme with diacylglycerol as substrate1 Publication
- Vmax=22.8 nmol/min/mg enzyme with 2-monoacylglycerol as substrate1 Publication
: triacylglycerol biosynthesis Pathwayi
This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.
GO - Molecular functioni
- 2-acylglycerol O-acyltransferase activity Source: UniProtKB
- diacylglycerol O-acyltransferase activity Source: UniProtKB
- O-acyltransferase activity Source: GO_Central
GO - Biological processi
- glycerol metabolic process Source: UniProtKB-KW
- lipid metabolic process Source: GO_Central
- monoacylglycerol biosynthetic process Source: UniProtKB
- triglyceride biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.22, 2681 |
PathwayCommonsi | Q86VF5 |
Reactomei | R-HSA-75109, Triglyceride biosynthesis |
UniPathwayi | UPA00282 |
Chemistry databases
SwissLipidsi | SLP:000000307 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-acylglycerol O-acyltransferase 3Curated (EC:2.3.1.201 Publication, EC:2.3.1.221 Publication)Alternative name(s): Acyl-CoA:monoacylglycerol acyltransferase 31 Publication Short name: MGAT31 Publication Diacylglycerol O-acyltransferase candidate 7 Short name: hDC7 Diacylglycerol acyltransferase 2-like protein 7 Monoacylglycerol O-acyltransferase 3 |
Gene namesi | ORF Names:UNQ9383/PRO34208 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23249, MOGAT3 |
MIMi | 610184, gene |
neXtProti | NX_Q86VF5 |
VEuPathDBi | HostDB:ENSG00000106384.10 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Curated
Other locations
- perinuclear region 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: UniProtKB
- perinuclear endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 29 – 49 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 50 – 70 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 137 – 157 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 265 | C → A: Reduces 60% 2-acylglycerol O-acyltransferase activity. No effect on diacylglycerol O-acyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 265 | C → Y: Catalitically inactive. No 2-acylglycerol O-acyltransferase neither diacylglycerol O-acyltransferase activities. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 346606 |
OpenTargetsi | ENSG00000106384 |
PharmGKBi | PA134959238 |
Miscellaneous databases
Pharosi | Q86VF5, Tbio |
Genetic variation databases
BioMutai | MOGAT3 |
DMDMi | 74727570 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249067 | 1 – 341 | 2-acylglycerol O-acyltransferase 3Add BLAST | 341 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 126 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Ubiquitinated. Ubiquitination leads to proteasomal degradation.1 Publication
Keywords - PTMi
Glycoprotein, Ubl conjugationProteomic databases
jPOSTi | Q86VF5 |
MassIVEi | Q86VF5 |
PaxDbi | Q86VF5 |
PeptideAtlasi | Q86VF5 |
PRIDEi | Q86VF5 |
ProteomicsDBi | 70001 [Q86VF5-1] 70002 [Q86VF5-2] 70003 [Q86VF5-3] |
PTM databases
GlyGeni | Q86VF5, 1 site |
Expressioni
Tissue specificityi
Selectively expressed in the digestive system. Highly expressed in the ileum, and at lower level in jejunum, duodenum, colon, cecum and the rectum. Not expressed in the stomach and the esophagus and trachea. Expressed at very low level in liver.1 Publication
Gene expression databases
Bgeei | ENSG00000106384, Expressed in mucosa of transverse colon and 33 other tissues |
Genevisiblei | Q86VF5, HS |
Organism-specific databases
HPAi | ENSG00000106384, Group enriched (intestine, liver) |
Interactioni
Binary interactionsi
Hide detailsIsoform 3 [Q86VF5-3]
Protein-protein interaction databases
BioGRIDi | 131392, 1 interactor |
IntActi | Q86VF5, 5 interactors |
STRINGi | 9606.ENSP00000223114 |
Miscellaneous databases
RNActi | Q86VF5, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the diacylglycerol acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01010000222418 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q86VF5 |
OMAi | VSHYHAL |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q86VF5 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q86VF5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGVATTLQPP TTSKTLQKQH LEAVGAYQYV LTFLFMGPFF SLLVFVLLFT
60 70 80 90 100
SLWPFSVFYL VWLYVDWDTP NQGGRRSEWI RNRAIWRQLR DYYPVKLVKT
110 120 130 140 150
AELPPDRNYV LGAHPHGIMC TGFLCNFSTE SNGFSQLFPG LRPWLAVLAG
160 170 180 190 200
LFYLPVYRDY IMSFGLCPVS RQSLDFILSQ PQLGQAVVIM VGGAHEALYS
210 220 230 240 250
VPGEHCLTLQ KRKGFVRLAL RHGASLVPVY SFGENDIFRL KAFATGSWQH
260 270 280 290 300
WCQLTFKKLM GFSPCIFWGR GLFSATSWGL LPFAVPITTV VGRPIPVPQR
310 320 330 340
LHPTEEEVNH YHALYMTALE QLFEEHKESC GVPASTCLTF I
Sequence cautioni
The sequence AAD45832 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 265 | C → Y in AAI00954 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020361 | 224 – 281 | ASLVP…SWGLL → GPPHPRPPAPPPHRGGSQSL SRPLHDGPGAALRGAQGKLW GPRFHLPHLHLGLAAAFR in isoform 2. 1 PublicationAdd BLAST | 58 | |
Alternative sequenceiVSP_020362 | 282 – 341 | Missing in isoform 2. 1 PublicationAdd BLAST | 60 | |
Alternative sequenceiVSP_020363 | 291 – 341 | VGRPI…CLTFI → GECPPPGGRPPAAAWASGIP RPPVSLSLQWAAPSPSPSAS TPPRRKSITITPST in isoform 3. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY229854 mRNA Translation: AAO63579.1 AY358200 mRNA Translation: AAQ88567.1 AC004876 Genomic DNA Translation: AAD45832.1 Sequence problems. BC100953 mRNA Translation: AAI00954.1 BC100954 mRNA Translation: AAI00955.1 BC100955 mRNA Translation: AAI00956.1 |
CCDSi | CCDS5714.1 [Q86VF5-1] CCDS75643.1 [Q86VF5-2] |
RefSeqi | NP_001274076.1, NM_001287147.1 [Q86VF5-2] NP_835470.1, NM_178176.3 [Q86VF5-1] XP_005250366.1, XM_005250309.3 [Q86VF5-3] |
Genome annotation databases
Ensembli | ENST00000223114; ENSP00000223114; ENSG00000106384 [Q86VF5-1] ENST00000379423; ENSP00000368734; ENSG00000106384 [Q86VF5-2] ENST00000440203; ENSP00000403756; ENSG00000106384 [Q86VF5-3] |
GeneIDi | 346606 |
KEGGi | hsa:346606 |
UCSCi | uc003uyc.5, human [Q86VF5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY229854 mRNA Translation: AAO63579.1 AY358200 mRNA Translation: AAQ88567.1 AC004876 Genomic DNA Translation: AAD45832.1 Sequence problems. BC100953 mRNA Translation: AAI00954.1 BC100954 mRNA Translation: AAI00955.1 BC100955 mRNA Translation: AAI00956.1 |
CCDSi | CCDS5714.1 [Q86VF5-1] CCDS75643.1 [Q86VF5-2] |
RefSeqi | NP_001274076.1, NM_001287147.1 [Q86VF5-2] NP_835470.1, NM_178176.3 [Q86VF5-1] XP_005250366.1, XM_005250309.3 [Q86VF5-3] |
3D structure databases
SMRi | Q86VF5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 131392, 1 interactor |
IntActi | Q86VF5, 5 interactors |
STRINGi | 9606.ENSP00000223114 |
Chemistry databases
SwissLipidsi | SLP:000000307 |
PTM databases
GlyGeni | Q86VF5, 1 site |
Genetic variation databases
BioMutai | MOGAT3 |
DMDMi | 74727570 |
Proteomic databases
jPOSTi | Q86VF5 |
MassIVEi | Q86VF5 |
PaxDbi | Q86VF5 |
PeptideAtlasi | Q86VF5 |
PRIDEi | Q86VF5 |
ProteomicsDBi | 70001 [Q86VF5-1] 70002 [Q86VF5-2] 70003 [Q86VF5-3] |
Protocols and materials databases
Antibodypediai | 2307, 100 antibodies |
Genome annotation databases
Ensembli | ENST00000223114; ENSP00000223114; ENSG00000106384 [Q86VF5-1] ENST00000379423; ENSP00000368734; ENSG00000106384 [Q86VF5-2] ENST00000440203; ENSP00000403756; ENSG00000106384 [Q86VF5-3] |
GeneIDi | 346606 |
KEGGi | hsa:346606 |
UCSCi | uc003uyc.5, human [Q86VF5-1] |
Organism-specific databases
CTDi | 346606 |
DisGeNETi | 346606 |
GeneCardsi | MOGAT3 |
HGNCi | HGNC:23249, MOGAT3 |
HPAi | ENSG00000106384, Group enriched (intestine, liver) |
MIMi | 610184, gene |
neXtProti | NX_Q86VF5 |
OpenTargetsi | ENSG00000106384 |
PharmGKBi | PA134959238 |
VEuPathDBi | HostDB:ENSG00000106384.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01010000222418 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q86VF5 |
OMAi | VSHYHAL |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q86VF5 |
TreeFami | TF314707 |
Enzyme and pathway databases
UniPathwayi | UPA00282 |
BRENDAi | 2.3.1.22, 2681 |
PathwayCommonsi | Q86VF5 |
Reactomei | R-HSA-75109, Triglyceride biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 346606, 5 hits in 874 CRISPR screens |
GenomeRNAii | 346606 |
Pharosi | Q86VF5, Tbio |
PROi | PR:Q86VF5 |
RNActi | Q86VF5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000106384, Expressed in mucosa of transverse colon and 33 other tissues |
Genevisiblei | Q86VF5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOGT3_HUMAN | |
Accessioni | Q86VF5Primary (citable) accession number: Q86VF5 Secondary accession number(s): Q496A6 Q9UDW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families