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Protein

Serine/threonine-protein kinase pim-3

Gene

PIM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei170Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell cycle Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • protein autophosphorylation Source: GO_Central
  • protein phosphorylation Source: UniProtKB
  • regulation of mitotic cell cycle Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ86V86
SIGNORiQ86V86

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase pim-3 (EC:2.7.11.1)
Gene namesi
Name:PIM3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000198355.4
HGNCiHGNC:19310 PIM3
MIMi610580 gene
neXtProtiNX_Q86V86

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi415116
OpenTargetsiENSG00000198355
PharmGKBiPA134980758

Chemistry databases

ChEMBLiCHEMBL5407
GuidetoPHARMACOLOGYi2160

Polymorphism and mutation databases

BioMutaiPIM3
DMDMi215274221

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865331 – 326Serine/threonine-protein kinase pim-3Add BLAST326

Post-translational modificationi

Ubiquitinated, leading to proteasomal degradation.1 Publication
Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86V86
MaxQBiQ86V86
PaxDbiQ86V86
PeptideAtlasiQ86V86
PRIDEiQ86V86
ProteomicsDBi69976

PTM databases

iPTMnetiQ86V86
PhosphoSitePlusiQ86V86

Expressioni

Tissue specificityi

Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.3 Publications

Gene expression databases

BgeeiENSG00000198355
CleanExiHS_PIM3
GenevisibleiQ86V86 HS

Organism-specific databases

HPAiHPA068758

Interactioni

Subunit structurei

Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation. Interacts with SOCS6 (By similarity).By similarity

Protein-protein interaction databases

BioGridi136104, 1 interactor
IntActiQ86V86, 2 interactors
MINTiQ86V86
STRINGi9606.ENSP00000353824

Chemistry databases

BindingDBiQ86V86

Structurei

3D structure databases

ProteinModelPortaliQ86V86
SMRiQ86V86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119045
HOGENOMiHOG000231357
HOVERGENiHBG106681
InParanoidiQ86V86
KOiK08807
OMAiMDHLPVK
OrthoDBiEOG091G0IMW
PhylomeDBiQ86V86
TreeFamiTF320810

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR017348 PIM1/2/3
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037993 STPK_Pim-1, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q86V86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG
60 70 80 90 100
FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA
110 120 130 140 150
GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF
160 170 180 190 200
FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV
210 220 230 240 250
YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE
260 270 280 290 300
ILRGRLLFRR RVSPECQQLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGV
310 320
PESCDLRLCT LDPDDVASTT SSSESL
Length:326
Mass (Da):35,891
Last modified:November 25, 2008 - v3
Checksum:i41FDEFAC4367A162
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37E → K in AAI41856 (PubMed:15489334).Curated1
Sequence conflicti300V → A in BAD42438 (PubMed:15540201).Curated1
Sequence conflicti300V → A in BAF84694 (PubMed:16141072).Curated1
Sequence conflicti300V → A in AAI41856 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114795 mRNA Translation: BAD42438.1
AK292005 mRNA Translation: BAF84694.1
CR536608 Genomic DNA No translation available.
BX539320 Genomic DNA No translation available.
BC052239 mRNA No translation available.
BC141855 mRNA Translation: AAI41856.1
CCDSiCCDS33678.1
RefSeqiNP_001001852.2, NM_001001852.3
UniGeneiHs.530381

Genome annotation databases

EnsembliENST00000360612; ENSP00000353824; ENSG00000198355
GeneIDi415116
KEGGihsa:415116
UCSCiuc003bjb.4 human

Similar proteinsi

Entry informationi

Entry nameiPIM3_HUMAN
AccessioniPrimary (citable) accession number: Q86V86
Secondary accession number(s): A5D8X8
, A8K7J0, B1B0P0, Q68BM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 25, 2008
Last modified: June 20, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

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