UniProtKB - Q86V81 (THOC4_HUMAN)
THO complex subunit 4
ALYREF
Functioni
Miscellaneous
GO - Molecular functioni
- C5-methylcytidine-containing RNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA 3'-end processing Source: Reactome
- mRNA export from nucleus Source: UniProtKB
- mRNA splicing, via spliceosome Source: Reactome
- osteoblast differentiation Source: UniProtKB
- positive regulation of DNA-templated transcription, elongation Source: UniProtKB
- regulation of DNA recombination Source: UniProtKB
- replication fork processing Source: UniProtKB
- RNA export from nucleus Source: UniProtKB
- viral mRNA export from host cell nucleus Source: UniProtKB
- viral process Source: UniProtKB-KW
Keywordsi
Molecular function | Chaperone, RNA-binding |
Biological process | Host-virus interaction, mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q86V81 |
Reactomei | R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SIGNORi | Q86V81 |
Names & Taxonomyi
Protein namesi | Recommended name: THO complex subunit 4Short name: Tho4 Alternative name(s): Ally of AML-1 and LEF-1 Aly/REF export factor Transcriptional coactivator Aly/REF bZIP-enhancing factor BEF |
Gene namesi | Name:ALYREF Synonyms:ALY, BEF, THOC4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19071, ALYREF |
MIMi | 604171, gene |
neXtProti | NX_Q86V81 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication4 Publications
- Nucleus speckle 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (PubMed:19324961). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).2 Publications
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- catalytic step 2 spliceosome Source: UniProtKB
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- transcription export complex Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 10189 |
PharmGKBi | PA134925107 |
Miscellaneous databases
Pharosi | Q86V81, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4296014 |
Genetic variation databases
BioMutai | ALYREF |
DMDMi | 48429165 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000081974 | 2 – 257 | THO complex subunit 4Add BLAST | 256 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources2 Publications | 1 | |
Modified residuei | 8 | PhosphoserineCombined sources | 1 | |
Modified residuei | 38 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 38 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 58 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 63 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 71 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 86 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 94 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | CitrullineBy similarity | 1 | |
Modified residuei | 197 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 197 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 204 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 204 | Dimethylated arginine; alternateCombined sources1 Publication | 1 | |
Modified residuei | 204 | Omega-N-methylarginine; alternate1 Publication | 1 | |
Modified residuei | 220 | Omega-N-methylarginine1 Publication | 1 | |
Modified residuei | 235 | N6-methyllysineCombined sources | 1 | |
Modified residuei | 239 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Citrullination, Methylation, PhosphoproteinProteomic databases
EPDi | Q86V81 |
jPOSTi | Q86V81 |
MassIVEi | Q86V81 |
MaxQBi | Q86V81 |
PaxDbi | Q86V81 |
PeptideAtlasi | Q86V81 |
PRIDEi | Q86V81 |
ProteomicsDBi | 69973 |
TopDownProteomicsi | Q86V81 |
PTM databases
iPTMneti | Q86V81 |
MetOSitei | Q86V81 |
PhosphoSitePlusi | Q86V81 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homomultimer. Is part of several complexes involved in mRNA processing and export.
Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; TREX seems to have a dynamic structure involving ATP-dependent remodeling; in the complex interacts (via C-terminus) directly with DDX39B and interacts directly with THOC1 and THOC2.
Found in mRNA splicing-dependent exon junction complexes (EJC). Identified in the spliceosome C complex.
Found in a mRNP complex with UPF3A and UPF3B.
Interacts with RBM8A, NCBP1, THOC5, LEF1, RUNX1, EIF4A3, RNPS1, SRRM1, IWS1 and EXOSC1.
Interacts with RBM15B (PubMed:19586903).
Interacts with NXF1; the interaction is direct.
21 Publications(Microbial infection) Interacts with human Kaposi's sarcoma-associated herpesvirus (HHV-8) ORF57 protein; this interaction allows efficient export of HHV-8 early and late intronless transcripts.
2 Publications(Microbial infection) Interacts with HHV-1 ICP27 protein; this interaction recruits ALYREF to viral replication compartments and probably directs viral mRNA to the TAP/NFX1 pathway.
2 PublicationsBinary interactionsi
Hide detailsQ86V81
Protein-protein interaction databases
BioGRIDi | 115486, 277 interactors |
CORUMi | Q86V81 |
DIPi | DIP-32711N |
IntActi | Q86V81, 131 interactors |
MINTi | Q86V81 |
STRINGi | 9606.ENSP00000421592 |
Miscellaneous databases
RNActi | Q86V81, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q86V81 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 106 – 183 | RRMPROSITE-ProRule annotationAdd BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 16 – 37 | Sufficient for RNA-binding, interaction with NXF1-NXT1 heterodimerAdd BLAST | 22 | |
Regioni | 85 – 186 | Interaction with HHV-8 ORF57 protein and with ICP27 from HHV-1By similarityAdd BLAST | 102 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 21 – 231 | Ala/Arg/Gly-richAdd BLAST | 211 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0533, Eukaryota |
InParanoidi | Q86V81 |
OrthoDBi | 1369069at2759 |
PhylomeDBi | Q86V81 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025715, FoP_C IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF13865, FoP_duplication, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM01218, FoP_duplication, 1 hit SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MADKMDMSLD DIIKLNRSQR GGRGGGRGRG RAGSQGGRGG GAQAAARVNR
60 70 80 90 100
GGGPIRNRPA IARGAAGGGG RNRPAPYSRP KQLPDKWQHD LFDSGFGGGA
110 120 130 140 150
GVETGGKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVHYD RSGRSLGTAD
160 170 180 190 200
VHFERKADAL KAMKQYNGVP LDGRPMNIQL VTSQIDAQRR PAQSVNRGGM
210 220 230 240 250
TRNRGAGGFG GGGGTRRGTR GGARGRGRGA GRNSKQQLSA EELDAQLDAY
NARMDTS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PB61 | E9PB61_HUMAN | THO complex subunit 4 | ALYREF | 264 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | G → R in AAD09608 (PubMed:9952027).Curated | 1 | |
Sequence conflicti | 35 – 36 | QG → RA in AAD09608 (PubMed:9952027).Curated | 2 | |
Sequence conflicti | 39 | G → R in AAD09608 (PubMed:9952027).Curated | 1 | |
Sequence conflicti | 150 | D → N in AAD09608 (PubMed:9952027).Curated | 1 | |
Sequence conflicti | 169 | V → F in AAD09608 (PubMed:9952027).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC145207 Genomic DNA No translation available. BC052302 mRNA Translation: AAH52302.1 AF047002 mRNA Translation: AAD09608.1 |
RefSeqi | NP_005773.3, NM_005782.3 |
Genome annotation databases
GeneIDi | 10189 |
KEGGi | hsa:10189 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC145207 Genomic DNA No translation available. BC052302 mRNA Translation: AAH52302.1 AF047002 mRNA Translation: AAD09608.1 |
RefSeqi | NP_005773.3, NM_005782.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ULH | X-ray | 2.54 | A | 78-183 | [»] | |
SMRi | Q86V81 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115486, 277 interactors |
CORUMi | Q86V81 |
DIPi | DIP-32711N |
IntActi | Q86V81, 131 interactors |
MINTi | Q86V81 |
STRINGi | 9606.ENSP00000421592 |
Chemistry databases
ChEMBLi | CHEMBL4296014 |
PTM databases
iPTMneti | Q86V81 |
MetOSitei | Q86V81 |
PhosphoSitePlusi | Q86V81 |
Genetic variation databases
BioMutai | ALYREF |
DMDMi | 48429165 |
Proteomic databases
EPDi | Q86V81 |
jPOSTi | Q86V81 |
MassIVEi | Q86V81 |
MaxQBi | Q86V81 |
PaxDbi | Q86V81 |
PeptideAtlasi | Q86V81 |
PRIDEi | Q86V81 |
ProteomicsDBi | 69973 |
TopDownProteomicsi | Q86V81 |
Protocols and materials databases
ABCDi | Q86V81, 1 sequenced antibody |
DNASUi | 10189 |
Genome annotation databases
GeneIDi | 10189 |
KEGGi | hsa:10189 |
Organism-specific databases
CTDi | 10189 |
DisGeNETi | 10189 |
GeneCardsi | ALYREF |
HGNCi | HGNC:19071, ALYREF |
MIMi | 604171, gene |
neXtProti | NX_Q86V81 |
PharmGKBi | PA134925107 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0533, Eukaryota |
InParanoidi | Q86V81 |
OrthoDBi | 1369069at2759 |
PhylomeDBi | Q86V81 |
Enzyme and pathway databases
PathwayCommonsi | Q86V81 |
Reactomei | R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SIGNORi | Q86V81 |
Miscellaneous databases
BioGRID-ORCSi | 10189, 675 hits in 1002 CRISPR screens |
ChiTaRSi | ALYREF, human |
GeneWikii | THOC4 |
GenomeRNAii | 10189 |
Pharosi | Q86V81, Tbio |
PROi | PR:Q86V81 |
RNActi | Q86V81, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025715, FoP_C IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF13865, FoP_duplication, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM01218, FoP_duplication, 1 hit SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THOC4_HUMAN | |
Accessioni | Q86V81Primary (citable) accession number: Q86V81 Secondary accession number(s): O43672 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families