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Protein

Acetoacetyl-CoA synthetase

Gene

AACS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).By similarity

Catalytic activityi

ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-77111 Synthesis of Ketone Bodies

Names & Taxonomyi

Protein namesi
Recommended name:
Acetoacetyl-CoA synthetase (EC:6.2.1.16)
Alternative name(s):
Acyl-CoA synthetase family member 1
Protein sur-5 homolog
Gene namesi
Name:AACS
Synonyms:ACSF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000081760.16
HGNCiHGNC:21298 AACS
MIMi614364 gene
neXtProtiNX_Q86V21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000081760
PharmGKBiPA134940696

Polymorphism and mutation databases

BioMutaiAACS
DMDMi74750446

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003157841 – 672Acetoacetyl-CoA synthetaseAdd BLAST672

Proteomic databases

EPDiQ86V21
MaxQBiQ86V21
PaxDbiQ86V21
PeptideAtlasiQ86V21
PRIDEiQ86V21
ProteomicsDBi69950
69951 [Q86V21-2]
69952 [Q86V21-3]

PTM databases

iPTMnetiQ86V21
PhosphoSitePlusiQ86V21

Expressioni

Tissue specificityi

Highly expressed in kidney, heart and brain, but low in liver.1 Publication

Gene expression databases

BgeeiENSG00000081760 Expressed in 226 organ(s), highest expression level in parotid gland
CleanExiHS_AACS
ExpressionAtlasiQ86V21 baseline and differential
GenevisibleiQ86V21 HS

Organism-specific databases

HPAiHPA058815

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CMTM5Q96DZ93EBI-10259474,EBI-2548702

Protein-protein interaction databases

BioGridi122434, 7 interactors
IntActiQ86V21, 1 interactor
STRINGi9606.ENSP00000324842

Structurei

3D structure databases

ProteinModelPortaliQ86V21
SMRiQ86V21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00900000141060
HOVERGENiHBG059947
InParanoidiQ86V21
KOiK01907
OMAiYEYVYRC
OrthoDBiEOG091G05B0
PhylomeDBiQ86V21
TreeFamiTF354241

Family and domain databases

InterProiView protein in InterPro
IPR005914 Acac_CoA_synth
IPR032387 ACAS_N
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
TIGRFAMsiTIGR01217 ac_ac_CoA_syn, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q86V21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSKEERPGRE EILECQVMWE PDSKKNTQMD RFRAAVGAAC GLALESYDDL
60 70 80 90 100
YHWSVESYSD FWAEFWKFSG IVFSRVYDEV VDTSKGIADV PEWFKGSRLN
110 120 130 140 150
YAENLLRHKE NDRVALYIAR EGKEEIVKVT FEELRQEVAL FAAAMRKMGV
160 170 180 190 200
KKGDRVVGYL PNSEHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ
210 220 230 240 250
PKLIFSVEAV VYNGKEHNHM EKLQQVVKGL PDLKKVVVIP YVSSRENIDL
260 270 280 290 300
SKIPNSVFLD DFLATGTSEQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM
310 320 330 340 350
VHSAGGTLIQ HLKEHLLHGN MTSSDILLCY TTVGWMMWNW MVSLLATGAA
360 370 380 390 400
MVLYDGSPLV PTPNVLWDLV DRIGITVLVT GAKWLSVLEE KAMKPVETHS
410 420 430 440 450
LQMLHTILST GSPLKAQSYE YVYRCIKSSI LLGSISGGTD IISCFMGHNF
460 470 480 490 500
SLPVYKGEIQ ARNLGMAVEA WNEEGKAVWG ESGELVCTKP IPCQPTHFWN
510 520 530 540 550
DENGNKYRKA YFSKFPGIWA HGDYCRINPK TGGIVMLGRS DGTLNPNGVR
560 570 580 590 600
FGSSEIYNIV ESFEEVEDSL CVPQYNKYRE ERVILFLKMA SGHAFQPDLV
610 620 630 640 650
KRIRDAIRMG LSARHVPSLI LETKGIPYTL NGKKVEVAVK QIIAGKAVEQ
660 670
GGAFSNPETL DLYRDIPELQ GF
Length:672
Mass (Da):75,144
Last modified:June 1, 2003 - v1
Checksum:i36006739EE4857D9
GO
Isoform 2 (identifier: Q86V21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     561-672: ESFEEVEDSL...YRDIPELQGF → YAQRQESGSC...RDPGSVPGHP

Note: No experimental confirmation available.
Show »
Length:599
Mass (Da):67,035
Checksum:i1C454CA0635CB5C5
GO
Isoform 3 (identifier: Q86V21-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-402: Missing.

Note: No experimental confirmation available.
Show »
Length:270
Mass (Da):30,061
Checksum:i930E1C6E82B5061D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EW25E7EW25_HUMAN
Acetoacetyl-CoA synthetase
AACS
218Annotation score:

Sequence cautioni

The sequence AAH41000 differs from that shown. Reason: Frameshift at position 201.Curated
The sequence BAB14793 differs from that shown. Reason: Frameshift at position 519.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70G → E in BAB14793 (PubMed:14702039).Curated1
Sequence conflicti119A → V in BAB14040 (PubMed:14702039).Curated1
Sequence conflicti128K → E in BAB14793 (PubMed:14702039).Curated1
Sequence conflicti137E → G in BAB14040 (PubMed:14702039).Curated1
Sequence conflicti150V → A in BAB14040 (PubMed:14702039).Curated1
Sequence conflicti391K → E in AAH41000 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038303118I → V1 PublicationCorresponds to variant dbSNP:rs11549081Ensembl.1
Natural variantiVAR_060997470A → V. Corresponds to variant dbSNP:rs59883951Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0307011 – 402Missing in isoform 3. 1 PublicationAdd BLAST402
Alternative sequenceiVSP_030702561 – 672ESFEE…ELQGF → YAQRQESGSCRQTDHRWKSR GARRCFLEPRDPGSVPGHP in isoform 2. 1 PublicationAdd BLAST112

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054121 mRNA Translation: BAD22560.1
AK022451 mRNA Translation: BAB14040.1
AK024036 mRNA Translation: BAB14793.1 Frameshift.
CH471054 Genomic DNA Translation: EAW98474.1
BC004997 mRNA Translation: AAH04997.1
BC040490 mRNA Translation: AAH40490.2
BC040574 mRNA Translation: AAH40574.1
BC041000 mRNA Translation: AAH41000.1 Frameshift.
BC051862 mRNA Translation: AAH51862.1
AY091468 mRNA Translation: AAM44124.1
AL833047 mRNA Translation: CAH56309.1
CCDSiCCDS9263.1 [Q86V21-1]
RefSeqiNP_001306768.1, NM_001319839.1 [Q86V21-2]
NP_076417.2, NM_023928.4 [Q86V21-1]
UniGeneiHs.656073

Genome annotation databases

EnsembliENST00000316519; ENSP00000324842; ENSG00000081760 [Q86V21-1]
GeneIDi65985
KEGGihsa:65985
UCSCiuc001uhc.4 human [Q86V21-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054121 mRNA Translation: BAD22560.1
AK022451 mRNA Translation: BAB14040.1
AK024036 mRNA Translation: BAB14793.1 Frameshift.
CH471054 Genomic DNA Translation: EAW98474.1
BC004997 mRNA Translation: AAH04997.1
BC040490 mRNA Translation: AAH40490.2
BC040574 mRNA Translation: AAH40574.1
BC041000 mRNA Translation: AAH41000.1 Frameshift.
BC051862 mRNA Translation: AAH51862.1
AY091468 mRNA Translation: AAM44124.1
AL833047 mRNA Translation: CAH56309.1
CCDSiCCDS9263.1 [Q86V21-1]
RefSeqiNP_001306768.1, NM_001319839.1 [Q86V21-2]
NP_076417.2, NM_023928.4 [Q86V21-1]
UniGeneiHs.656073

3D structure databases

ProteinModelPortaliQ86V21
SMRiQ86V21
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122434, 7 interactors
IntActiQ86V21, 1 interactor
STRINGi9606.ENSP00000324842

PTM databases

iPTMnetiQ86V21
PhosphoSitePlusiQ86V21

Polymorphism and mutation databases

BioMutaiAACS
DMDMi74750446

Proteomic databases

EPDiQ86V21
MaxQBiQ86V21
PaxDbiQ86V21
PeptideAtlasiQ86V21
PRIDEiQ86V21
ProteomicsDBi69950
69951 [Q86V21-2]
69952 [Q86V21-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316519; ENSP00000324842; ENSG00000081760 [Q86V21-1]
GeneIDi65985
KEGGihsa:65985
UCSCiuc001uhc.4 human [Q86V21-1]

Organism-specific databases

CTDi65985
EuPathDBiHostDB:ENSG00000081760.16
GeneCardsiAACS
H-InvDBiHIX0022598
HGNCiHGNC:21298 AACS
HPAiHPA058815
MIMi614364 gene
neXtProtiNX_Q86V21
OpenTargetsiENSG00000081760
PharmGKBiPA134940696
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00900000141060
HOVERGENiHBG059947
InParanoidiQ86V21
KOiK01907
OMAiYEYVYRC
OrthoDBiEOG091G05B0
PhylomeDBiQ86V21
TreeFamiTF354241

Enzyme and pathway databases

ReactomeiR-HSA-77111 Synthesis of Ketone Bodies

Miscellaneous databases

ChiTaRSiAACS human
GenomeRNAii65985
PROiPR:Q86V21
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000081760 Expressed in 226 organ(s), highest expression level in parotid gland
CleanExiHS_AACS
ExpressionAtlasiQ86V21 baseline and differential
GenevisibleiQ86V21 HS

Family and domain databases

InterProiView protein in InterPro
IPR005914 Acac_CoA_synth
IPR032387 ACAS_N
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
TIGRFAMsiTIGR01217 ac_ac_CoA_syn, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAACS_HUMAN
AccessioniPrimary (citable) accession number: Q86V21
Secondary accession number(s): Q49AB9
, Q49AC3, Q658Q8, Q8IWD2, Q8NEW5, Q9BSJ9, Q9H829, Q9HA19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: September 12, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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